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Your search keyword '"Liu, Wensen"' showing total 27 results
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27 results on '"Liu, Wensen"'

25. CircEpc1 Promotes Ricin Toxin-Induced Inflammation via Activation of NF-κB and MAPK Signaling Pathways by Sponging miR-5114

Author

Dong, Mingxin, primary, Zhang, Xiaohao, additional, Yu, Haotian, additional, Wang, Yan, additional, Chang, Ying, additional, Sun, Chengbiao, additional, Zhang, Jianxu, additional, Zhao, Na, additional, Yu, Kaikai, additional, Sun, Guangchao, additional, Zhao, Guiru, additional, Xu, Na, additional, and Liu, Wensen, additional

Published
2021
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26. Ultrasensitive detection of pathogenic bacteria by CRISPR/Cas12a coupling with a primer exchange reaction

Author

Xiu Liu, Hongguo Wei, Liu Wensen, Songling Yu, Jiayu Wan, Bu Shengjun, Ze Wang, Zhuo Hao, and Zhongyi Li

Subjects
biology, Metals and Alloys, Pathogenic bacteria, Condensed Matter Physics, biology.organism_classification, medicine.disease_cause, Primer extension, Surfaces, Coatings and Films, Electronic, Optical and Magnetic Materials, chemistry.chemical_compound, Biochemistry, chemistry, Materials Chemistry, medicine, Nucleic acid, CRISPR, Electrical and Electronic Engineering, Primer (molecular biology), Instrumentation, Escherichia coli, Bacteria, DNA
Abstract

The detection of pathogenic bacteria is extremely important to maintain food safety. In the present study, we constructed an electrochemical biosensor for point-of-care testing (POCT) of pathogenic bacteria through the cascade signal amplification of CRISPR/Cas12a and primer exchange reaction (PER) without nucleic acid extraction. Functional DNA aptamers locked the hairpin of PER, thereby preventing primer extension in the absence of target pathogenic bacteria. The presence of target pathogenic bacteria triggered the unlocking of the hairpin of PER and extended the primer into a long single-stranded DNA (ssDNA), which then activated the cleavage of ssDNA modified on an Au electrode by Cas12a, resulting in the decrease of the electrochemical signal detectable by an electrochemical workstation. The sensor could quantify Escherichia coli O157:H7 concentration from 10 to 106 CFU mL−1, with a detection limit of 19 CFU mL−1. Target bacteria in milk samples can be successfully detected by this method. On the basis of these characteristics, the proposed electrochemical biosensor based on CRISPR/Cas12a and PER could serve as a prospective tool for new point-of-care (POC) applications in food safety.

Published
2021

27. Expression of Toll-like receptors in the cerebellum during pathogenesis of prion disease.

Author

Liao X, Zhu W, Liao X, Liu W, Hou Y, and Wan J

Abstract

Prion diseases, such as scrapie, entail the accumulation of disease-specific prion protein ( PrP Sc ) within the brain. Toll-like receptors (TLRs) are crucial components of the pattern recognition system. They recognize pathogen-associated molecular patterns (PAMPs) and play a central role in orchestrating host innate immune responses. The expression levels of Toll-like receptors (TLRs) in the central nervous system (CNS) were not well-defined. To establish a model of prion diseases in BALB/C mice, the 22L strain was employed. The features of the 22L strain were analyzed, and the cerebellum exhibited severe pathological changes. TLR1-13 levels in the cerebellum were measured using quantitative polymerase chain reaction (qPCR) at time points of 60, 90, 120, and the final end point (145 days post-infection). During the pathogenesis, the expression levels of Toll-like receptors (TLRs) 1, 2, 7, 8, and 9 increased in a time-dependent manner. This trend mirrored the expression patterns of PrP Sc (the pathological isoform of the prion protein) and glial fibrillary acidic protein. Notably, at the end point, TLR1-13 levels were significantly elevated. Protein level of TLR7 and TLR9 showed increasing at the end point of the 22L-infected mice. A deeper understanding of the increased Toll-like receptors (TLRs) in prion diseases could shed light on their role in initiating immune responses at various stages during pathogenesis. This insight is particularly relevant when considering TLRs as potential therapeutic targets for prion diseases., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2024 Liao, Zhu, Liao, Liu, Hou and Wan.)

Published
2024
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