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This study aimed to investigate effects of heat treatment temperatures (60℃, 70℃, 80℃, 90℃, 100℃) and times (10 min, 30 min, 60 min) on the aggregation behavior of Moringa Oleiferaseed salt-soluble protein (MOSP). With the increase of heating temperature and time, the MOSP solutions were changed from a more transparent state to a cloudy and opaque state, the absorbance value of the MOSP solution increased significantly, and the particle size gradually increased to a maximum of 1220.33 nm (100℃, 30 min). The polymer dispersion index (PDI) also increased and the maximum PDI of MOSP (100℃, 10 min) was 0.53, but the absolute value of the zeta-potential was gradually decreased. With the increase of heating temperature and heating time, the β-sheet content of MOSP was increased to 30.8%, while the α-helix content of MOSP decreased. There was no obvious change in the subunit distribution of the heat-induced MOSP. The spectrum analysis displayed that heat treatment led to the exposure of the hydrophobic residues in MOSP, but as the heating temperature was further increased to 90℃, re-embedding of the hydrophobic groups was observed due to the formation of aggregates. The total sulfhydryl group (-SHT) increased with the increase of temperature, while the free sulfhydryl group (-SHF)first decreased and then increased during short-term heating (10 and 30 min). The maximum content of -SHFreached 4.40% and the content of disulfide bonds was 11.97% (90℃, 30 min). These changes could have a significant impact on protein products and might spur innovation in new protein applications.

BackgroundComputed tomography perfusion (CTP) parameters have been shown to have predictive value for functional outcomes of patients with basilar artery occlusion (BAO). We report the predictive value of CTP-based software (CTP-Rapid Processing of Perfusion and Diffusion (RAPID); iSchemia View) for functional outcomes of patients with BAO after endovascular therapy (EVT).MethodsPatients with BAO who underwent EVT were retrospectively analyzed in our center from December 2019 to July 2021. Baseline characteristics and imaging parameters from non-contrast CT, CT angiography (CTA), and CTP-RAPID were collected for analysis.ResultsAmong the 55 patients enrolled in this study, 22 (40.0%) achieved a good functional outcome (modified Rankin Scale score ≤3 at 90 days). In the univariate analysis, posterior circulation Alberta Stroke Program Early CT Score, Basilar Artery on CT Angiography score, posterior circulation CTA score, posterior communicating artery deficiency, perfusion deficit volume in time to maximum (Tmax) >4 s, Tmax >6 s, and mismatch volume were associated with functional outcomes (all p<0.05). In the multivariate analysis, perfusion deficit volume in Tmax >6 s (OR 1.011 (95% CI 1.001 to 1.020)) and posterior circulation CTA score (OR 0.435 (95% CI 0.225 to 0.840)) remained independent outcome predictors (all p<0.05).ConclusionsPerfusion deficit volume in Tmax >6 s on CTP-RAPID imaging maps and basilar artery on CTA score have potential as functional outcome predictors in patients with BAO after EVT.