Search

The assertive online strategies of Portuguese luxury fashion brands/designers (LFBDs) are essential for managers to succeed in the digital medium and establish long-lasting relationships with clients. This paper explores the challenges in digitizing all the distinctive elements of a luxury fashion brand: the product characteristics, the luxury atmosphere, the personalization of the service, and the services provided. Fourteen Portuguese brands/designers were selected for content analysis. According to Hansen’s framework, 43% of Portuguese LFBDs fail to share information about their companies on their websites, and only 43% include promotions. Only 36% have a community outside the websites, compared to 93% of the international luxury fashion brands. Moreover, Portuguese websites lack 360° technology and augmented reality. Considering the Digital Visual Merchandising – MVD Grid, Portuguese LFBDs do not allow multiple zooms and 2D F/T cursor change, while international brands feature these tools. Regarding the Social Media Performance Analysis Grid, the global average engagement rate of Portuguese LFBDs on Facebook is 0.19% and on Instagram – 0.89%. According to Social Media Content Analysis Grid, Portuguese brands bet a lot on sharing content in story format, mainly on Instagram; however, Facebook has a higher network value. Therefore, the findings show that Portuguese LFBDs should be present and market their products online. This online presence must be supported by a multi-channel strategy while maintaining luxury product characteristics and elements of differentiation. AcknowledgmentThis study is partially financed by the Portuguese national funds through FCT – Fundação para a Ciência e Tecnologia, under the project UIDB/05422/2020.

Background Jet fires and boiling liquid expanding vapour explosions (BLEVEs) are potential events when a vessel containing liquefied petroleum gas (LPG) is exposed to fire. Events involving domestic LPG tanks have occurred at wildland–urban interface areas in Portugal, the USA, Spain and Greece. Aims Evaluation of the pressure relief device (PRD), the type of cylinder and the effects of jet fires and BLEVEs. Methods LPG cylinders manufactured with steel and composite materials were exposed to fire. Hydrostatic tests were carried out to compare the burst pressure without fire influence. Fourteen accidents that occurred during wildfires are described. Key results The presence of a PRD and the right choice of type of cylinder as well as cylinder location may avert major accidents. The jet fires and fireball radiation are also described, as well as the maximum distance reached by cylinder fragments. Conclusions The tests showed that the burst pressure in the test of a cylinder without PRD under fire was significantly lower when compared with the hydrostatic tests. Implications Lastly, recommendations to avoid accidents are made.

Background In Pedrógão Grande on 17 June 2017, two fire fronts merged and the propagation of the fire was influenced by the interaction of these non-symmetric fire fronts. Aims This wildfire motivated us to study a junction fire with two non-symmetrical fire fronts. The analysis of the movement of the intersection point and the angle (γ) between the bisector of the fire lines and the maximum rate of spread (ROS) direction is of particular relevance. Methods The study was carried out at Forest Fire Laboratory of the University of Coimbra in Lousã (Portugal) with laboratory experiments. Key results We found that, for small rotation angles (δ), the non-dimensional ROS of the intersection point depends on the slope angle (α) and the initial angle between fire fronts. Conclusions For high α, the non-dimensional ROS was highly influenced by the convection process and γ where the maximum ROS occurred, increased when δ increased. However, the radiation process was more relevant for lower α and influenced the non-dimensional ROS. For these cases, the maximum spread direction was close to that of the fire line bisector. Implications The present work aimed to explain fire behaviour during the Pedrógão Grande wildfire.

We control phase separation/gelation inside of emulsion droplets to reproducibly achieve SiO2–TiO2 based microspheres with tailored interconnected coral-like macroporosity. These microspheres (MICROSCAFS®) are key enablers of emergent applications.

Under strain control tests and cyclic loading, extruded magnesium alloys exhibit a special mechanism of plastic deformation (“twinning” and “de-twining”). As a result, magnesium alloys exhibit an asymmetric material behavior that cannot be fully characterized with the typical numerical tools used for steels or aluminum alloys. In this sense, a new phenomenological model, called hypo-strain, has been developed to correctly predict the cyclic stress–strain evolution of magnesium alloys. On this basis, this work aims to accurately describe the local cyclic elastic–plastic behavior of AZ31B-F magnesium alloy under multiaxial cyclic loading with Abaqus incremental plasticity. The phenomenological hypo-strain model was implemented in the UMAT subroutine of Abaqus/Standard to be used as a design tool for mechanical design. To evaluate this phenomenological approach, the results were correlated with the uniaxial and multiaxial proportional and non-proportional experimental tests. In addition, the estimates were also correlated with the Armstrong–Frederick nonlinear kinematic hardening model. The results show a good correlation between the experiments and the phenomenological hypo strain approach. The model and its implementation were validated in the strain range studied.

Background Chagas disease (CD), caused by the parasite Trypanosoma cruzi, is a serious public health concern in Latin America. Nifurtimox and benznidazole (BZ), the only two drugs currently approved for the treatment of CD, have very low efficacies in the chronic phase of the disease and several toxic side effects. Trypanosoma cruzi strains that are naturally resistant to both drugs have been reported. We performed a comparative transcriptomic analysis of wild-type and BZ-resistant T. cruzi populations using high-throughput RNA sequencing to elucidate the metabolic pathways related to clinical drug resistance and identify promising molecular targets for the development of new drugs for treating CD. Methods All complementary DNA (cDNA) libraries were constructed from the epimastigote forms of each line, sequenced and analysed using the Prinseq and Trimmomatic tools for the quality analysis, STAR as the aligner for mapping the reads against the reference genome (T. cruzi Dm28c—2018), the Bioconductor package EdgeR for statistical analysis of differential expression and the Python-based library GOATools for the functional enrichment analysis. Results The analytical pipeline with an adjusted P-value of < 0.05 and fold-change > 1.5 identified 1819 transcripts that were differentially expressed (DE) between wild-type and BZ-resistant T. cruzi populations. Of these, 1522 (83.7%) presented functional annotations and 297 (16.2%) were assigned as hypothetical proteins. In total, 1067 transcripts were upregulated and 752 were downregulated in the BZ-resistant T. cruzi population. Functional enrichment analysis of the DE transcripts identified 10 and 111 functional categories enriched for the up- and downregulated transcripts, respectively. Through functional analysis we identified several biological processes potentially associated with the BZ-resistant phenotype: cellular amino acid metabolic processes, translation, proteolysis, protein phosphorylation, RNA modification, DNA repair, generation of precursor metabolites and energy, oxidation–reduction processes, protein folding, purine nucleotide metabolic processes and lipid biosynthetic processes. Conclusions The transcriptomic profile of T. cruzi revealed a robust set of genes from different metabolic pathways associated with the BZ-resistant phenotype, proving that T. cruzi resistance mechanisms are multifactorial and complex. Biological processes associated with parasite drug resistance include antioxidant defenses and RNA processing. The identified transcripts, such as ascorbate peroxidase (APX) and iron superoxide dismutase (Fe-SOD), provide important information on the resistant phenotype. These DE transcripts can be further evaluated as molecular targets for new drugs against CD. Graphical Abstract

This work aims to produce a cartographic model that identifies areas with a greater probability of finding karstic caves - the endokarst potential - in the northern sector of Santo António Plateau (Estremadura Limestone Massif, Central Portugal). Geological, topographic, hydrogeological, and vegetation cover data were collected, processed, and integrated into a spatial database using a geographic information system (GIS). The location of known cave entrances in the study area was also identified from the records of local public institutions and speleological teams. Four conditioning factors were extracted from the collected data, including lithostratigraphic units, fracture density, relief energy, and land cover. In a multi-criteria decision-making analysis framework, each previously chosen conditioning factor and respective classes/categories were weighted using the analytic hierarchy process (AHP). With an agreement of 81.9%, the results of the cartographic model constructed in GIS seem to be promising, considering that the entrances of the known caves are mainly located in areas classified as having high to very high endokarst potential. This prototype of the endokarst potential map for the study area can be used in strategic and operational environmental planning (at least on a local scale), as it can assist decision-makers, competent authorities, and local speleological teams in a more accurate and thoughtful definition of the areas that should be investigated, providing a substantial reduction in times and costs field prospecting.

Avian metapneumovirus (aMPV) causes a highly contagious upper respiratory and reproductive disease in chickens, turkeys, and ducks. Here, complete genome sequences of aMPV-B vaccine strains BR/1890/E1/19 (PL21, Nemovac; Boehringer Ingelheim Animal Health, Brazil) and BR/1891/E2/19 (1062; Hipraviar, France) were sequenced and compared with the pathogenic field strain VCO3/60616.

Cellular structures are formed by struts and edges, which makes them difficult to fabricate by conventional technologies. The emergence of 3D printing has enabled the production of such complex structures. In the present work, the Fused Filament Fabrication method was used to obtain cellular structures formed by the repetition of triply periodic minimal surfaces unit cells. Due to the complexity of the cells, several defects occurred until the procedure was fine-tuned. Failure analysis of samples was performed after bending and compression tests. Failure occurs at the same locations of the samples independently of their relative density, for the three densities tested. These fracture initiation locations correspond to the zones where the von Mises stress is highest.

Leishmania are kinetoplastid pathogens that cause leishmaniasis, a debilitating and potentially life-threatening infection if untreated. Unusually, Leishmania regulate their gene expression largely post-transcriptionally due to the arrangement of their coding genes into polycistronic transcription units that may contain 100s of functionally unrelated genes. Yet, Leishmania are capable of rapid and responsive changes in gene expression to challenging environments, often instead correlating with dynamic changes in their genome composition, ranging from chromosome and gene copy number variations to the generation of extrachromosomal DNA and the accumulation of point mutations. Typically, such events indicate genome instability in other eukaryotes, coinciding with genetic abnormalities, but for Leishmania, exploiting these products of genome instability can provide selectable substrates to catalyse necessary gene expression changes by modifying gene copy number. Unorthodox DNA replication, DNA repair, replication stress factors and DNA repeats are recognised in Leishmania as contributors to this intrinsic instability, but how Leishmania regulate genome plasticity to enhance fitness whilst limiting toxic under- or over-expression of co-amplified and co-transcribed genes is unclear. Herein, we focus on fresh, and detailed insights that improve our understanding of genome plasticity in Leishmania. Furthermore, we discuss emerging models and factors that potentially circumvent regulatory issues arising from polycistronic transcription. Lastly, we highlight key gaps in our understanding of Leishmania genome plasticity and discuss future studies to define, in higher resolution, these complex regulatory interactions.

Additive manufacturing (AM) of polymer large parts is a technological research area with great growth potential if the main barriers to its implementation are successfully addressed. In this research, a review of large-format AM (LFAM) processes for polymers is presented, followed by market research concerning the identification of large-format polymer commercial printers. An overview was performed covering the current LFAM systems configurations and their control aspects. The design and modelling approaches related with the fabrication of polymer large parts by AM, and the materials currently being applied and under development, were described. Finally, a summary of LFAM applications with a focus in the Transportation, Academic, Construction and Energy sectors, was presented. The current main advances in the LFAM of polymers are linked with the possibility of producing large parts in a faster, cheaper, and reliable way. The market research analysis concerning results for all AM families involving polymer materials reveals that, currently, the material extrusion AM process family is potentially the most suitable to produce large parts, with a significant number of applications attesting its capability to produce such large-format components.

Repetitive elements cause assembly fragmentation in complex eukaryotic genomes, limiting the study of their variability. The genome of Trypanosoma cruzi, the parasite that causes Chagas disease, has a high repetitive content, including multigene families. Although many T. cruzi multigene families encode surface proteins that play pivotal roles in host-parasite interactions, their variability is currently underestimated, as their high repetitive content results in collapsed gene variants. To estimate sequence variability and copy number variation of multigene families, we developed a read-based approach that is independent of gene-specific read mapping and

/SummaryBackgroundVisceral leishmaniasis (VL) is a potentially fatal disease mainly caused by Leishmania infantum in South America and L. donovani in Asia and Africa. Disease outcomes have been associated with patient genotype, nutrition, age, sex, comorbidities, and co-infections. In this study, we examine the effects of parasite genetic variation on VL disease severity in Brazil.MethodsWe collected and sequenced the genomes of 109 L. infantum isolates from patients in northeast Brazil and retrieved matching patient clinical data from medical records, including mortality, sex, HIV co-infection and laboratory data (creatinine, haemoglobin, leukocyte and platelet counts). We identified genetic differences between parasite isolates, including single nucleotide polymorphisms (SNPs), small insertions/deletions (indels), and variations in genic, intergenic, and chromosome copy numbers (copy number variants, CNVs). To describe associations between the parasite genotypes and clinical outcomes, we applied quantitative genetics methods of heritability and genome-wide association studies (GWAS), treating clinical outcomes as traits that may be influenced by parasite genotype.FindingsMultiple aspects of the genetic analysis indicate that parasite genotype affects clinical outcomes. We estimate that parasite genotype explains 83% chance of mortality (narrow sense heritability, h2 = 0·83±0·17), and has a significant relationship with patient sex (h2 = 0·60±0·27). Impacts of parasite genotype on other clinical traits are lower (h2 ≤0·34). GWAS analysis identified multiple parasite genetic loci that were significantly associated with clinical outcomes; 17 CNVs that were significantly associated with mortality, two with creatinine and one with bacterial co-infection, jaundice and HIV co-infection; and two SNPs/indels and six CNVs that associate with age, jaundice, HIV and bacterial co-infections, creatinine, and/or bleeding sites.InterpretationParasite genotype is an important factor in VL disease severity in Brazil. Our analysis indicates that specific genetic differences between parasites act as virulence factors, enhancing risks of severe disease and mortality. More detailed understanding of these virulence factors could be exploited for novel therapies.Author SummaryMultiple factors contribute to the risk of mortality from visceral leishmaniasis (VL), including, patient genotype, comorbidities, and nutrition. Many of these factors will be influenced by socio-economic biases 1. Our work suggests that the virulence of the infecting parasite is an important risk factor for mortality. We pinpoint some specific genomic markers that are associated with mortality, which can lead to a greater understanding of the molecular mechanisms that cause severe VL disease, to genetic markers for virulent parasites and to the development of drug and vaccine therapies.