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Your search keyword '"Lucas, Mark"' showing total 9 results
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9 results on '"Lucas, Mark"'

1. Massively scaled-up testing for SARS-CoV-2 RNA via next-generation sequencing of pooled and barcoded nasal and saliva samples.

Author

Bloom, Joshua, Sathe, Laila, Munugala, Chetan, Jones, Eric, Gasperini, Molly, Lubock, Nathan, Yarza, Fauna, Thompson, Erin, Kovary, Kyle, Park, Jimin, Marquette, Dawn, Kay, Stephania, Lucas, Mark, Love, TreQuan, Sina Booeshaghi, A, Brandenberg, Oliver, Guo, Longhua, Boocock, James, Hochman, Myles, Simpkins, Scott, Lin, Isabella, LaPierre, Nathan, Hong, Duke, Zhang, Yi, Oland, Gabriel, Choe, Bianca, Chandrasekaran, Sukantha, Hilt, Evann, Butte, Manish, Damoiseaux, Robert, Kravit, Clifford, Cooper, Aaron, Yin, Yi, Pachter, Lior, Garner, Omai, Flint, Jonathan, Eskin, Eleazar, Luo, Chongyuan, Kosuri, Sriram, Kruglyak, Leonid, and Arboleda, Valerie

Subjects
High-Throughput Nucleotide Sequencing, Humans, RNA, Viral, SARS-CoV-2, Saliva, Sensitivity and Specificity
Abstract

Frequent and widespread testing of members of the population who are asymptomatic for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is essential for the mitigation of the transmission of the virus. Despite the recent increases in testing capacity, tests based on quantitative polymerase chain reaction (qPCR) assays cannot be easily deployed at the scale required for population-wide screening. Here, we show that next-generation sequencing of pooled samples tagged with sample-specific molecular barcodes enables the testing of thousands of nasal or saliva samples for SARS-CoV-2 RNA in a single run without the need for RNA extraction. The assay, which we named SwabSeq, incorporates a synthetic RNA standard that facilitates end-point quantification and the calling of true negatives, and that reduces the requirements for automation, purification and sample-to-sample normalization. We used SwabSeq to perform 80,000 tests, with an analytical sensitivity and specificity comparable to or better than traditional qPCR tests, in less than two months with turnaround times of less than 24 h. SwabSeq could be rapidly adapted for the detection of other pathogens.

Published
2021

3. E-cadherin in developing murine T cells controls spindle alignment and differentiation during β-selection

Author

Patrick Humbert, Amr Allam, Sarah Russell, Mirren Charnley, and Lucas Mark Newton

Abstract

A critical stage of T cell development is β-selection; at this stage the TCRβ chain is generated and the developing T cell starts to acquire antigenic specificity. Progression through β-selection is assisted by a low affinity interaction between the nascent TCRβ chain and peptide presented on stromal MHC and external cues provided by the niche, including Notch and CXCR4. In this study, we reveal the importance of a new cue within the murine developing T cell niche which is critical for T cell development. E-cadherin mediates cell-cell interactions and influences cell fate in many developmental systems. In developing T cells E-cadherin contributed to the formation of an immunological synapse and the alignment of the mitotic spindle with the polarity axis during division, which facilitated subsequent T cell development. Collectively, these data highlight a new aspect of the developing T cell niche and provide insights into the role of E-cadherin in the β-selection stage of T cell development.

Published
2022

4. M-Link Data Linkage Ethics and Governance Guide

Author

Brown, Dianne, Andrew, Nadine E, Kilkenny, Monique F, Del Monaco, Anthony, Ilomaki, Jenni, Nielsen, Suzanne, Lucas, Mark, Gabbe, Belinda, Beare, Richard, and Fincher, Megan

Subjects
Public health not elsewhere classified
Abstract

A guide produced by M-Link (part of Monash University Helix) for researchers wanting to apply to use Australian government data sets (both State and Commonwealth). Includes suggestions on how to navigate the ethics and governance application process.

Published
2022
Full Text
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5. Helix Health Research Data Dictionary Template

Author

Brown, Dianne, Sparks, Simone, Earnest, Arul, Lucas, Mark, Thompson, Robin, and Macmanus, Chris

Subjects
education, 111799 Public Health and Health Services not elsewhere classified, FOS: Health sciences
Abstract

As part of Monash University (Helix) Health Research Data Governance strategy a working group was established in 2019 to develop a data dictionary template.This is an excel version of the template that can be output into a word document. It contains all the metadata (characteristics) that should be included in a health research data dictionary in a standardised format.

Published
2022
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7. Dominion of destruction.

Author

Lucas, Mark

Abstract

REGULARS & COLUMNSNature is struggling to survive for one reason: man ("Dear green place", November). Remove man from the scene and nature will re-establish itself within a surprisingly few short years. It is man's activities that destroy all. We are too many and our impact is overwhelming. We believe we have dominion over the fauna and flora: we fool ourselves.Write to us…Email letters@prospectmagazine.co.uk. Letters require a full name and address and may be edited…or join the debate onlineComments are open on the Prospect website. Visit prospectmagazine.co.uk to continue the conversation [Extracted from the article]

Published
2024

8. LETTERS.

Author

Johns, Chris, Reed, Peter, Park, Simon, Woods, David R., Ayyad, Mohammad, Grenfell, Scott, Manley, Nick, Lucas, Mark, Bussey, Paul, Austin, David, McMillan, Grant, Borgloh, Peter, Smith, Tim, and Thalmann, Dennis

Subjects
PROBLEM solving
Abstract

One page describes the company and its products converted from other brands, such as Austin, Honda, BMW, Mercedes and the Range Rover estates mentioned in your story. I keep thinking that I might try a BMW Mini now… David R Woods Via email Arabian knight The article about the missing Rapport Forté convertible having been found and being restored came as a relief (Lost & found, September), and made me recollect that an Arabic car magazine called Sport Auto wrote about the car when it was new, exactly four decades earlier in October 1981. I admit to being somewhat biased, having started my career as an Austin apprentice the year the Maxi was launched: you can imagine the impression such an innovative car made on a budding engineer. The aftermarket purchase of a spray shield (a small piece of bitumen-coated cardboard) solved this problem, years before it was fitted as standard; I was surprised to notice this essential item missing in the underbonnet photo of Bolster's car. [Extracted from the article]

Published
2021

9. LETTERS.

Author

Lucas, Mark, Phillips, Marc, Buckden, David, Short, Phil, Day, Tom, Renner, Dave, Roberts, Clive, Wells, Charles, Haag, Nick, David, Jen, Proud, Graham, Hayter, Nigel, Mordey, Alan, and Sait, Andrew

Subjects
INFORMATION-seeking behavior
Abstract

All of the cars raced on street-spec radial tyres, and Johnson figured that the Gremlin and Hornet, with their six-cylinder engines, could be contenders against the BMW 2002s, Ford Pintos, Volvo 122s and other production sedans sold in the US, as per the rules. Johnson was the creator of Team Highball, using factory-sponsored American MotorsGremlinsandHornets, and went on to race AMC Spirit AMXs in the 1979 Nürburgring 24 Hours before switching to Mazda. Letter of the month Crossing continents aboard the ultimate family transport The Fiat Multipla article (July) and owner John Hewett's dream of driving back to Italy with his son in their fabulous restoration evoked enormous nostalgia for this 66-yearold. These days, as Simon points out, youngsters grow up with sat-nav on their phones or in their cars, and geography is now taught with little appreciation of the knowledge maps can impart. [Extracted from the article]

Published
2021

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