12 results on '"Lucchini F."'
Search Results
2. Clinical presentation and prognosis of COVID-19 in older adults with hypothyroidism: data from the GeroCovid observational study
- Author
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Bagala, V, Sala, A, Trevisan, C, Okoye, C, Incalzi, R, Monzani, F, Volpato, S, Antognoli, R, Antonietti, M, Bandini, G, Bellelli, G, Benvenuti, E, Bergamin, M, Bertolotti, M, Biagini, C, Bianchetti, A, Bianchi, A, Bianchi, M, Bignamini, S, Blandini, D, Boffelli, S, Bugada, M, Calsolaro, V, Calvani, D, Carpagnano, E, Carrieri, B, Castaldo, V, Cavarape, A, Cazzulani, I, Celesti, C, Ceolin, C, Ceresini, M, Cherubini, A, Chizzoli, A, Ciarrocchi, E, Cicciomessere, P, Coin, A, Corsi, A, Custodero, C, D'Agostino, F, D'Errico, M, De Iorio, A, De Marchi, A, Desideri, G, Di Matteo, E, Espinosa, E, Esposito, L, Fazio, C, Filippini, C, Fiore, L, Fontana, C, Forte, L, Montorzi, R, Fumagalli, C, Fumagalli, S, Gareri, P, Giordano, A, Giuliani, E, Greco, A, Herbst, A, Ielo, G, La Marca, A, Porta, U, Lazzari, I, Lelli, D, Longobucco, Y, Lucchini, F, Lucente, D, Maestri, L, Maggio, M, Mainqua, P, Marengoni, A, Martin, B, Massa, V, Mazza, L, Mazzoccoli, C, Mossello, E, Morellini, F, Mussi, C, Orio, G, Paglia, A, Pelagalli, G, Pelizzoni, L, Picci, A, Ranhoff, A, Remelli, F, Resta, O, Riccardi, A, Rinaldi, D, Rozzini, R, Sabba, C, Sacco, L, Santoliquido, M, Savino, M, Scarso, F, Sergi, G, Serviddio, G, Sidoli, C, Solfrizzi, V, Soli, B, Tafaro, L, Tedde, A, Testa, G, Tinti, M, Tonarelli, F, Tonon, E, Vitali, A, Zoccarato, F, Zotti, S, Bagala V., Sala A., Trevisan C., Okoye C., Incalzi R. A., Monzani F., Volpato S., Antognoli R., Antonietti M. P., Bandini G., Bellelli G., Benvenuti E., Bergamin M., Bertolotti M., Biagini C. A., Bianchetti A., Bianchi A., Bianchi M., Bignamini S., Blandini D., Boffelli S., Bugada M., Calsolaro V., Calvani D., Carpagnano E., Carrieri B., Castaldo V., Cavarape A., Cazzulani I., Celesti C., Ceolin C., Ceresini M. G., Cherubini A., Chizzoli A., Ciarrocchi E., Cicciomessere P., Coin A., Corsi A., Custodero C., D'Agostino F., D'Errico M. M., De Iorio A., De Marchi A., Desideri G., Di Matteo E., Espinosa E., Esposito L., Fazio C., Filippini C., Fiore L., Fontana C., Forte L., Montorzi R. F., Fumagalli C., Fumagalli S., Gareri P., Giordano A., Giuliani E., Greco A., Herbst A., Ielo G., La Marca A., Porta U., Lazzari I., Lelli D., Longobucco Y., Lucchini F., Lucente D., Maestri L., Maggio M., Mainqua P., Marengoni A., Martin B., Massa V., Mazza L., Mazzoccoli C., Mossello E., Morellini F., Mussi C., Orio G., Paglia A., Pelagalli G., Pelizzoni L., Picci A., Ranhoff A. H., Remelli F., Resta O., Riccardi A., Rinaldi D., Rozzini R., Sabba C., Sacco L., Santoliquido M., Savino M., Scarso F., Sergi G., Serviddio G., Sidoli C., Solfrizzi V., Soli B., Tafaro L., Tedde A., Testa G. D., Tinti M. G., Tonarelli F., Tonon E., Vitali A., Zoccarato F., Zotti S., Bagala, V, Sala, A, Trevisan, C, Okoye, C, Incalzi, R, Monzani, F, Volpato, S, Antognoli, R, Antonietti, M, Bandini, G, Bellelli, G, Benvenuti, E, Bergamin, M, Bertolotti, M, Biagini, C, Bianchetti, A, Bianchi, A, Bianchi, M, Bignamini, S, Blandini, D, Boffelli, S, Bugada, M, Calsolaro, V, Calvani, D, Carpagnano, E, Carrieri, B, Castaldo, V, Cavarape, A, Cazzulani, I, Celesti, C, Ceolin, C, Ceresini, M, Cherubini, A, Chizzoli, A, Ciarrocchi, E, Cicciomessere, P, Coin, A, Corsi, A, Custodero, C, D'Agostino, F, D'Errico, M, De Iorio, A, De Marchi, A, Desideri, G, Di Matteo, E, Espinosa, E, Esposito, L, Fazio, C, Filippini, C, Fiore, L, Fontana, C, Forte, L, Montorzi, R, Fumagalli, C, Fumagalli, S, Gareri, P, Giordano, A, Giuliani, E, Greco, A, Herbst, A, Ielo, G, La Marca, A, Porta, U, Lazzari, I, Lelli, D, Longobucco, Y, Lucchini, F, Lucente, D, Maestri, L, Maggio, M, Mainqua, P, Marengoni, A, Martin, B, Massa, V, Mazza, L, Mazzoccoli, C, Mossello, E, Morellini, F, Mussi, C, Orio, G, Paglia, A, Pelagalli, G, Pelizzoni, L, Picci, A, Ranhoff, A, Remelli, F, Resta, O, Riccardi, A, Rinaldi, D, Rozzini, R, Sabba, C, Sacco, L, Santoliquido, M, Savino, M, Scarso, F, Sergi, G, Serviddio, G, Sidoli, C, Solfrizzi, V, Soli, B, Tafaro, L, Tedde, A, Testa, G, Tinti, M, Tonarelli, F, Tonon, E, Vitali, A, Zoccarato, F, Zotti, S, Bagala V., Sala A., Trevisan C., Okoye C., Incalzi R. A., Monzani F., Volpato S., Antognoli R., Antonietti M. P., Bandini G., Bellelli G., Benvenuti E., Bergamin M., Bertolotti M., Biagini C. A., Bianchetti A., Bianchi A., Bianchi M., Bignamini S., Blandini D., Boffelli S., Bugada M., Calsolaro V., Calvani D., Carpagnano E., Carrieri B., Castaldo V., Cavarape A., Cazzulani I., Celesti C., Ceolin C., Ceresini M. G., Cherubini A., Chizzoli A., Ciarrocchi E., Cicciomessere P., Coin A., Corsi A., Custodero C., D'Agostino F., D'Errico M. M., De Iorio A., De Marchi A., Desideri G., Di Matteo E., Espinosa E., Esposito L., Fazio C., Filippini C., Fiore L., Fontana C., Forte L., Montorzi R. F., Fumagalli C., Fumagalli S., Gareri P., Giordano A., Giuliani E., Greco A., Herbst A., Ielo G., La Marca A., Porta U., Lazzari I., Lelli D., Longobucco Y., Lucchini F., Lucente D., Maestri L., Maggio M., Mainqua P., Marengoni A., Martin B., Massa V., Mazza L., Mazzoccoli C., Mossello E., Morellini F., Mussi C., Orio G., Paglia A., Pelagalli G., Pelizzoni L., Picci A., Ranhoff A. H., Remelli F., Resta O., Riccardi A., Rinaldi D., Rozzini R., Sabba C., Sacco L., Santoliquido M., Savino M., Scarso F., Sergi G., Serviddio G., Sidoli C., Solfrizzi V., Soli B., Tafaro L., Tedde A., Testa G. D., Tinti M. G., Tonarelli F., Tonon E., Vitali A., Zoccarato F., and Zotti S.
- Abstract
Background: The prevalence of hypothyroidism among older patients hospitalized for COVID-19 and its association with mortality is unclear. This study aims to investigate the prevalence of hypothyroidism in older COVID-19 inpatients and verify if this comorbidity is associated with a specific pattern of onset symptoms and a worse prognosis. Methods: COVID-19 inpatients aged ≥ 60 years, participating in the GeroCovid acute wards cohort, were included. The history of hypothyroidism was derived from medical records and the use of thyroid hormones. Sociodemographic data, comorbidities, symptoms/signs at the disease onset and inflammatory markers at ward admission were compared between people with vs without history of hypothyroidism. The association between hypothyroidism and in-hospital mortality was tested through Cox regression. Results: Of the 1245 patients included, 8.5% had a history of hypothyroidism. These patients were more likely to present arterial hypertension and obesity compared with those without an history of hypothyroidism. Concerning COVID-19 clinical presentation, patients with hypothyroidism had less frequently low oxygen saturation and anorexia but reported muscle pain and loss of smell more commonly than those without hypothyroidism. Among the inflammatory markers, patients with hypothyroidism had higher lymphocytes values. At Cox regression, hypothyroidism was associated with reduced in-hospital mortality only in the univariable model (HR = 0.66, 95% CI 0.45–0.96, p = 0.03); conversely, no significant result were observed after adjusting for potential confounders (HR = 0.69, 95% CI 0.47–1.03, p = 0.07). Conclusions: Hypothyroidism does not seem to substantially influence the prognosis of COVID-19 in older people, although it may be associated with peculiar clinical and biochemical features at the disease onset.
- Published
- 2023
3. Social Costs of Gambling Harm in Italy
- Author
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Lucchini, F, Comi, S, Lucchini F., Comi S. L., Lucchini, F, Comi, S, Lucchini F., and Comi S. L.
- Abstract
The aim of this study is to provide an estimate of the social costs of gambling in Italy. In line with other research on social costs, the present study estimates the consequences of gambling harm on public finances, focusing on the estimated costs to treat high-risk gamblers, costs associated with productivity losses, costs of unemployment, personal and family costs, crime and legal costs. We used two different approaches to calculate these costs. The first approach, used for health care costs, consists of using the lump sum spent to prevent the harm caused to high-risk gamblers. The second approach involves estimating the number of high-risk gamblers causing the cost, which is then multiplied with the average unit cost per person. Our estimates of the annual social costs of gambling in Italy – more than EUR 2.3 billion – demonstrate a substantial economic burden to society. However, the costs are a substantial underestimate, as they are limited to those of a public nature and do not take into consideration those costs borne by moderate and low-risk gamblers, as well as affected others.
- Published
- 2022
4. siRNAs pools generated in Escherichia coli exhibit strong RNA-interference activity against influenza virus genomic sequences
- Author
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Villa, R., Renzi, S., Dotti, S., Lucchini, Franco, Lucchini F. (ORCID:0000-0003-0280-7062), Villa, R., Renzi, S., Dotti, S., Lucchini, Franco, and Lucchini F. (ORCID:0000-0003-0280-7062)
- Abstract
Due to the recurrent pandemic outbreaks that occurred during the last century, Influenza A viruses are considered a serious potential danger to human health. Among the innate immune pathways in eukaryotes, RNA interference plays a significant role in the interaction between viruses and host cells. RNA interference is addressed by small dsRNA molecules produced by the host itself (miRNAs, i.e. "micro-RNAs") but can be triggered also by the administration of exogenous short RNAs (siRNAs, "short interfering RNAs"). In this work, artificial siRNA pools targeting NP and PB genomic regions of the Influenza virus were produced in engineered Escherichia coli, adapting a published protocol. In a MDCK cell in vitro model, these preparations were challenged against reporter vectors bearing viral genomic sequences. A strong and specific RNA interference activity was observed, and the details of this action were indagated.
- Published
- 2023
5. La città come entità politica
- Author
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Tumminelli Giuseppina, Sgrò, G, Vinci, F, Maestro, A, Morrone, G, Massimilla, E, Di Biasem, R, Mirabelli, M, Veltri, F, Scafoglio, L, Macaluso, M, Lucchini, F, Rech, G, Tumminelli, G, and Tumminelli Giuseppina
- Subjects
Settore SPS/11 - Sociologia Dei Fenomeni Politici ,Città, città globali, potere - Abstract
Il saggio di Max Weber "La città" (1921) fu inserito dal curatore dell’opera di Weber, Johannes Winckelmann, nella seconda parte di Economia e società relativa alle forme del potere e del dominio (Herrschaft) con il titolo "La città: tipologia del potere non-legittimo". Nel testo, partendo dalla domanda conoscitiva che cosa sia la città, si cerca di comprendere se, nella contemporaneità, possa essere ancora valido il modello di città proposto dal sociologo.
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- 2022
6. Chromosome Transplantation: Opportunities and Limitations.
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La Grua A, Rao I, Susani L, Lucchini F, Raimondi E, Vezzoni P, and Paulis M
- Subjects
- Animals, Humans, Genetic Therapy methods, Male, Female, Synthetic Biology methods, Chromosomes, Genomic Medicine
- Abstract
There are thousands of rare genetic diseases that could be treated with classical gene therapy strategies such as the addition of the defective gene via viral or non-viral delivery or by direct gene editing. However, several genetic defects are too complex for these approaches. These "genomic mutations" include aneuploidies, intra and inter chromosomal rearrangements, large deletions, or inversion and copy number variations. Chromosome transplantation (CT) refers to the precise substitution of an endogenous chromosome with an exogenous one. By the addition of an exogenous chromosome and the concomitant elimination of the endogenous one, every genetic defect, irrespective of its nature, could be resolved. In the current review, we analyze the state of the art of this technique and discuss its possible application to human pathology. CT might not be limited to the treatment of human diseases. By working on sex chromosomes, we showed that female cells can be obtained from male cells, since chromosome-transplanted cells can lose either sex chromosome, giving rise to 46,XY or 46,XX diploid cells, a modification that could be exploited to obtain female gametes from male cells. Moreover, CT could be used in veterinary biology, since entire chromosomes containing an advantageous locus could be transferred to animals of zootechnical interest without altering their specific genetic background and the need for long and complex interbreeding. CT could also be useful to rescue extinct species if only male cells were available. Finally, the generation of "synthetic" cells could be achieved by repeated CT into a recipient cell. CT is an additional tool for genetic modification of mammalian cells.
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- 2024
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7. A new, unusually large, Clavicornaltica Scherer, 1974 flea beetle from Borneo, described and sequenced in the field by citizen scientists (Coleoptera, Chrysomelidae, Galerucinae).
- Author
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Otani S, Bertoli L, Lucchini F, van den Beuken TPG, Boin D, Ellis L, Friedrich H, Jacquot B, Kountouras S, Lim SYR, Nigro E, Su'eif S, Tan WH, Grafe U, Cicuzza D, Delledonne M, Njunjić I, and Schilthuizen M
- Abstract
Background: The genus Clavicornaltica Scherer 1974 consists of very small, soil-dwelling flea beetles in South, Southeast and East Asia. Due to their diminutive size and morphological similarities, very little is known about their ecology and taxonomical diversity. It is likely that further studies will reveal this genus to be much more speciose than the 30 species currently recognised., New Information: A new species of Clavicornaltica from Brunei Darussalam is described, C.mataikanensis Otani et al., sp. nov. This is the second species of this genus recorded from Ulu Temburong National Park., (Sean Otani, Luca Bertoli, Filippo Lucchini, Tom P. G. van den Beuken, Desanne Boin, Lehman Ellis, Holm Friedrich, Brittany Jacquot, Sotiris Kountouras, Sarah Yu Rou Lim, Eleonora Nigro, Syafi’ie Su’eif, Wei Harn Tan, Ulmar Grafe, Daniele Cicuzza, Massimo Delledonne, Iva Njunjić, Menno Schilthuizen.)
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- 2024
- Full Text
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8. Prevalence of Problem Gambling: A Meta-analysis of Recent Empirical Research (2016-2022).
- Author
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Gabellini E, Lucchini F, and Gattoni ME
- Subjects
- Adult, Humans, Prevalence, Cross-Sectional Studies, Gambling psychology, Behavior, Addictive epidemiology
- Abstract
Gambling is widely considered a socially acceptable form of recreation. However, for a small minority of individuals, it can become both addictive and problematic with severe adverse consequences. The aim of this systematic review and meta-analysis is to provide an overview of prevalence studies published between 2016 and the first quarter of 2022 and an updated estimate of problem gambling in the general adult population. A systematic review and a meta-analysis were carried out using academic databases, Internet, and governmental websites. Following this search and utilizing exclusion criteria, 23 studies on adult gambling prevalence were identified, distinguishing between moderate risk/at risk gambling and problem/pathological gambling. This study found a prevalence of moderate risk/at risk gambling to be 2.43% and of problem/pathological gambling to be 1.29% in the adult population. As difficult as it may be to compare studies due to different methodological procedures, cutoffs, and time frames, the present meta-analysis highlights the variations of prevalence across different countries, giving due consideration to the differences between levels of risk and severity. This work intends to provide a starting point for policymakers and academics to fill the gaps on gambling research-more specifically in some countries where the lack of research in this field is evident-and to study the effectiveness of policies implemented to mitigate gambling harm., (© 2022. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)
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- 2023
- Full Text
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9. Phenolic acids, lignans, and low-molecular-weight phenolics exhibit the highest in vitro cellular bioavailability in different digested and faecal-fermented phenolics-rich plant extracts.
- Author
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García-Pérez P, Rocchetti G, Giuberti G, Lucchini F, and Lucini L
- Subjects
- Humans, Biological Availability, Caco-2 Cells, Digestion, Phenols chemistry, Polyphenols metabolism, Plant Extracts chemistry, Lignans
- Abstract
Polyphenols are multifaceted bioactive compounds, but little is known about their real impact on human health after consumption. In this work, the phenolic profiling of quebracho, yellow maize, and violet rice extracts was comprehensively investigated, together with the impact of in vitro digestion and colonic fermentation on the bioaccessibility and bioavailability of these phytochemicals. The different matrices showed distinct profiles, potentially influencing in vitro starch digestion under cooking conditions. Furthermore, after the extracts underwent in vitro gastrointestinal digestion and faecal fermentation, phenolics exhibited a differential bioaccessibility trend at every digestion level, with matrix-dependent behaviour. The bioavailability results suggest that polyphenols are metabolised during colonic fermentation, mainly into tyrosols, phenolic acids, and lignans, which are partially absorbed by Caco-2 cells. By combining metabolomics with in vitro cellular methods, this research provides new insights into the fate of these phytochemicals in the gut, yielding comprehensive data on their consumption in food matrices., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier Ltd. All rights reserved.)
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- 2023
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10. CRISPR-Cas9-based repeat depletion for high-throughput genotyping of complex plant genomes.
- Author
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Rossato M, Marcolungo L, De Antoni L, Lopatriello G, Bellucci E, Cortinovis G, Frascarelli G, Nanni L, Bitocchi E, Di Vittori V, Vincenzi L, Lucchini F, Bett KE, Ramsay L, Konkin DJ, Delledonne M, and Papa R
- Subjects
- Genotype, Genome, Plant, Genotyping Techniques, High-Throughput Nucleotide Sequencing methods, Sequence Analysis, DNA methods, Genome-Wide Association Study, CRISPR-Cas Systems
- Abstract
High-throughput genotyping enables the large-scale analysis of genetic diversity in population genomics and genome-wide association studies that combine the genotypic and phenotypic characterization of large collections of accessions. Sequencing-based approaches for genotyping are progressively replacing traditional genotyping methods because of the lower ascertainment bias. However, genome-wide genotyping based on sequencing becomes expensive in species with large genomes and a high proportion of repetitive DNA. Here we describe the use of CRISPR-Cas9 technology to deplete repetitive elements in the 3.76-Gb genome of lentil ( Lens culinaris ), 84% consisting of repeats, thus concentrating the sequencing data on coding and regulatory regions (single-copy regions). We designed a custom set of 566,766 gRNAs targeting 2.9 Gbp of repeats and excluding repetitive regions overlapping annotated genes and putative regulatory elements based on ATAC-seq data. The novel depletion method removed ∼40% of reads mapping to repeats, increasing those mapping to single-copy regions by ∼2.6-fold. When analyzing 25 million fragments, this repeat-to-single-copy shift in the sequencing data increased the number of genotyped bases of ∼10-fold compared to nondepleted libraries. In the same condition, we were also able to identify ∼12-fold more genetic variants in the single-copy regions and increased the genotyping accuracy by rescuing thousands of heterozygous variants that otherwise would be missed because of low coverage. The method performed similarly regardless of the multiplexing level, type of library or genotypes, including different cultivars and a closely related species ( L. orientalis ). Our results showed that CRISPR-Cas9-driven repeat depletion focuses sequencing data on single-copy regions, thus improving high-density and genome-wide genotyping in large and repetitive genomes., (© 2023 Rossato et al.; Published by Cold Spring Harbor Laboratory Press.)
- Published
- 2023
- Full Text
- View/download PDF
11. siRNAs pools generated in Escherichia coli exhibit strong RNA-interference activity against influenza virus genomic sequences.
- Author
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Villa R, Renzi S, Dotti S, and Lucchini F
- Subjects
- Escherichia coli genetics, Escherichia coli metabolism, Genomics, RNA Interference, RNA, Small Interfering genetics, RNA, Small Interfering metabolism, Madin Darby Canine Kidney Cells, Animals, Dogs, Influenza A virus genetics, Influenza A virus metabolism, MicroRNAs
- Abstract
Due to the recurrent pandemic outbreaks that occurred during the last century, Influenza A viruses are considered a serious potential danger to human health. Among the innate immune pathways in eukaryotes, RNA interference plays a significant role in the interaction between viruses and host cells. RNA interference is addressed by small dsRNA molecules produced by the host itself (miRNAs, i.e. "micro-RNAs") but can be triggered also by the administration of exogenous short RNAs (siRNAs, "short interfering RNAs"). In this work, artificial siRNA pools targeting NP and PB genomic regions of the Influenza virus were produced in engineered Escherichia coli, adapting a published protocol. In a MDCK cell in vitro model, these preparations were challenged against reporter vectors bearing viral genomic sequences. A strong and specific RNA interference activity was observed, and the details of this action were indagated., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier Inc. All rights reserved.)
- Published
- 2023
- Full Text
- View/download PDF
12. New insights into the lipidomic response of CaCo-2 cells to differently cooked and in vitro digested extra-virgin olive oils.
- Author
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Lozano-Castellón J, Rocchetti G, Vallverdú-Queralt A, Lucchini F, Giuberti G, Torrado-Prat X, Illán M, Mª Lamuela-Raventós R, and Lucini L
- Subjects
- Caco-2 Cells, Humans, Olive Oil, Oxidative Stress, Cooking methods, Lipidomics
- Abstract
The health benefits of extra virgin olive oil (EVOO) are far proven. However, considering that this oil is consumed also cooked, this work aimed to evaluate the impact of different cooking techniques on human colorectal adenocarcinoma cells (Caco-2) exposed to in vitro digested EVOO. In particular, the effect of different cooking methods, namely sauteing, deep-frying, and Roner®, was assayed and compared to a raw EVOO sample. The Caco-2 cell lysates were analyzed through an untargeted lipidomics approach, and multivariate statistics were used to identify the marker compounds of the differences in cells' lipidomic signatures. Despite representing the cooking at the lowest temperature (but longer time), cells exposed to Roner® cooked EVOO presented the most distinguished lipidomic profile. The markers of differences in Caco-2 could be related to oxidative stress-related compounds such as oxidized glutathione, diketogulonic acid, ceramides, and diglycerides. Taken together, our findings indicate that the differences in EVOO composition determined by cooking could impose significant lipidomic perturbation on the human intestinal cells., (Copyright © 2022. Published by Elsevier Ltd.)
- Published
- 2022
- Full Text
- View/download PDF
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