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Based on the integrated model of Super-SBM model, spatial Durbin model (SDM) and Grey neural network model, this paper analyzes the panel data of various provinces in China from multiple angles and dimensions. It was found that there were significant differences in eco-efficiency between organic rice production and conventional rice production. The response of organic rice to climate change, the spatial distribution of ecological and economic benefits and the impact on carbon emission were analyzed. The results showed that organic rice planting not only had higher economic benefits, but also showed a rising trend of ecological benefits and a positive feedback effect. This finding highlights the importance of organic rice farming in reducing carbon emissions. Organic rice farming effectively reduces greenhouse gas emissions, especially carbon dioxide and methane, by improving soil management and reducing the use of fertilizers and pesticides. This has important implications for mitigating climate change and promoting soil health and biodiversity. With the acceleration of urbanization, the increase of organic rice planting area shows the trend of organic rice gradually replacing traditional rice cultivation, further highlighting the potential of organic agriculture in emission reduction, environmental protection and sustainable agricultural production. To this end, it is recommended that the Government implement a diversified support strategy to encourage technological innovation, provide guidance and training, and raise public awareness and demand for organic products. At the same time, private sector participation is stimulated to support the development of organic rice cultivation through a public-private partnership model. Through these measures, further promote organic rice cultivation, achieve the dual goals of economic benefits and environmental benefits, and effectively promote the realization of double carbon emission reduction targets. [ABSTRACT FROM AUTHOR]

Knowledge of protein expression in mammalian brains at regional and cellular levels can facilitate understanding of protein functions and associated diseases. As the mouse brain is a typical mammalian brain considering cell type and structure, several studies have been conducted to analyze protein expression in mouse brains. However, labeling protein expression using biotechnology is costly and time-consuming. Therefore, automated models that can accurately recognize protein expression are needed. Here, we constructed machine learning models to automatically annotate the protein expression intensity and cellular location in different mouse brain regions from immunofluorescence images. The brain regions and sub-regions were segmented through learning image features using an autoencoder and then performing K-means clustering and registration to align with the anatomical references. The protein expression intensities for those segmented structures were computed on the basis of the statistics of the image pixels, and patch-based weakly supervised methods and multi-instance learning were used to classify the cellular locations. Results demonstrated that the models achieved high accuracy in the expression intensity estimation, and the F1 score of the cellular location prediction was 74.5%. This work established an automated pipeline for analyzing mouse brain images and provided a foundation for further study of protein expression and functions. [ABSTRACT FROM AUTHOR]

With rapid economic expansion, China is faced with environmental challenges like air pollution and greenhouse gas emissions. Shifting from conventional fossil fuels to renewable energy (REN) sources is critical to facilitate sustainable development in China. Compared to coal and oil, REN such as solar and wind energy emit less carbon emissions. Fostering innovation of REN technologies is thus essential for China's green transition. This study aims to analyze the impact of REN technology innovation on China's economic growth using panel data models. The results demonstrate that advancing REN technologies significantly promotes GDP increase in China. Targeted policy incentives must be implemented to accelerate REN technology progression and adoption across the country. Transitioning towards REN systems will be instrumental for China to achieve environmental sustainability while maintaining economic growth. [ABSTRACT FROM AUTHOR]

Objectives: To explore the underlying mechanism of total flavonoids of Litchi seed (TFLS) in treating prostate cancer (PCa). Methods: Cell Counting Kit-8 (CCK-8), EdU incorporation assay, trypan blue dye assay and colony formation assay were employed to evaluate the effect of TFLS on PCa in vitro. The xenograft mouse model was established to explore the anti-tumour effect of TFLS in vivo. Alterations in the metabolic profiles of the PC3 cells and mouse serum were obtained by untargeted metabolomics. Combination with metabolomics analysis and network pharmacology strategies, the potential targets were predicted and further validated by RT-qPCR. Key findings: TFLS attenuated PCa progression both in vitro and in vivo. Metabolomics results yielded from cells and serum indicated that the anti-cancer effect of TFLS was correlated with synergistic modulation of five common metabolic pathways including glycerophospholipid metabolism, arginine and proline metabolism, glycine, serine and threonine metabolism, tryptophan metabolism and steroid biosynthesis. Using in silico prediction and RT-qPCR analysis, we further revealed that TFLS exerted anti-PCa activities via regulating the expressions of nine genes, including MAOA, ACHE, ALDH2, AMD1, ARG1, PLA2G10, PLA2G1B, FDFT1 and SQLE. Conclusions: TFLS suppressed tumour proliferation in PCa, which may be associated with regulating lipid and amino acid metabolisms. [ABSTRACT FROM AUTHOR]

Although personal thermal management (PTM) materials for daily routine environments are widely investigated, the exploration of multifunctional PTM materials with excellent feasibility in complex and outdoor scenarios is still in its infancy. Herein, inspired by the temperature regulation effect of the atmosphere, a novel design for fabricating solar‐thermal gradient reduced graphene oxide (RGO) aerogel‐based bilayer phase change composite (GRGC) featuring "energy regulation" and "energy inverse compensation" is proposed for self‐adaptive PTM applications. By integrating the solar‐thermal energy conversion ability of RGO, the heat regulation of the aerogel/octadecane bilayer structure with the unique gradient RGO framework inside, and the latent‐heat compensation of octadecane, the bilayer GRGC can serve as an efficient PTM device to mitigate drastic temperature changes of human skin in harsh environments. This multifunctional PTM device can not only keep a warm skin surface microclimate in a frigid environment of −5 °C via the synergistic effect of the solar‐thermal conversion, the aerogel thermal insulation, and the phase‐change latent heat release, but also provide efficient thermal buffering to prevent hyperthermia in hot environments through its phase‐change behavior and its thermal insulation. This gradient and bilayer design opens a new avenue for fabricating self‐adaptive PTM devices for applications in harsh environments. [ABSTRACT FROM AUTHOR]

Under the current rice cropping system, excessive nitrogen application has become a major issue that needs to be changed, and nitrogen reduction has become a hot research topic in recent years. The use of optimum planting density is becoming a common agronomic management system in addition to nitrogen reduction, especially under double cropping rice systems. In this paper, changes in rice yield, nitrogen-use efficiency (NUE) and net N mineralization under dense planting with a reduced nitrogen rate (DPRN) were studied. By comparing DPRN with high-nitrogen sparse planting (SPHN), we found that the population tiller number (tiller number per unit area) increased by 9–27% under DPRN cultivation. Nitrogen accumulation under DPRN treatment of double-cropped rice was basically stable. NUE under DPRN was significantly higher by 1.3–22.7% compared to SPHN. The partial factor productivity of applied N (PFPN) was significantly higher than that of SPHN, with an increase of 4.3–22.8%. The net N mineralized of double-cropped rice under DPRN increased at different stages, and the increase in late-season rice (LSR) was greater than that of early-season rice (ESR). The highest net N mineralized in double cropping rice at different stages was found in the dense planting treatment (DP) and N2 (120 kg N h−1). In conclusion, DPRN cultivation of double-cropped rice could be accepted as a proper management strategy for reducing nitrogen input, improving NUE and promoting soil nitrogen mineralization under given conditions. [ABSTRACT FROM AUTHOR]

Nitrogen fertilization and planting density are two key factors that influence the yield of rice. Reducing nitrogen fertilizer input and increasing planting density will help to improve nitrogen use efficiency and stabilize yield. Field and 15N tracer method in plot experiments were conducted to study the trends of yield, nitrogen use efficiency (NUE) and nitrogen transfer of hybrid rice and conventional rice under dense planting with a reduced nitrogen rate (DPRN) and sparse planting with a high nitrogen rate (SPHN). Among the nitrogen in rice plants, the proportion of nitrogen from fertilizer under the DPRN was reduced by 1.8–13%. The late-season rice (LSR) had a higher rate of decrease compared with the early-season rice (ESR). The uptake efficiency of nitrogen fertilizer was significantly higher under the DPRN than that under the SPHN, with an increase of 7.7–21.9%. The accumulated nitrogen and translocated ratio under the DPRN before the heading stage were 6.1–10.8% and 2.0–9.6% higher than those under the SPHN, respectively. The yield did not change under different treatments. Those findings suggest that the DPRN could guarantee a stabilized yield while increasing the NUE and the amount of translocated nitrogen in the double-cropped rice system. [ABSTRACT FROM AUTHOR]

Heavy crude oil rich in colloid and asphaltene is widely used in human life, but the collection and clean-up of its spills are difficult because of its high viscosity and low flowability. Herein, the zeolitic imidazolate framework-8/reduced graphene oxide (ZIF-8/RGO) hybrid aerogels are prepared by in situ anchoring ZIF-8 particles onto anisotropic RGO aerogel for efficient clean-up of spilled heavy crude oil. The solar-thermal ZIF-8/RGO hybrid aerogel with numerous vertical microchannels is efficient in decreasing viscosity and improving flowability of heavy crude oil because of the high solar light absorption and solar-thermal energy conversion capabilities of the RGO and the increased solar light diffuse reflection path and hydrophobicity of the aerogel by ZIF-8. Under 1-sun irradiation, the surface temperature of the aerogel reaches rapidly to 70 °C within 1 min. At the same time, the viscosity of the heavy crude oil falls quickly from 105 to 103 mPa s, and the maximum adsorption capacity of heavy crude oil reaches 112 g g−1 within 9 min. Moreover, the hybrid aerogel exhibits satisfactory adsorption and cycling ability to various organic solvents and oily media. The excellent solar-thermal energy conversion and high adsorption capacities make the anisotropic aerogel promising for efficient clean-up of spilled heavy crude oil. [Display omitted] [ABSTRACT FROM AUTHOR]

Corydalis saxicola Bunting (CS), a traditional Chinese folk medicine, has been effectively used for treating liver disease in Zhuang nationality in South China. However, the main anti-liver fibrosis ingredients in CS are incompletely understood. To elucidate the main anti-liver fibrosis ingredients in CS and its underlying mechanism. Firstly, spectrum-effect relationship (SER) strategy was applied to identify the major ingredients against liver fibrosis in CS. Subsequently, 1H NMR metabonomics and metagenomics sequencing techniques were used to clarify the intervention of palmatine (PAL) on liver fibrosis. Furthermore, the expression of tight junction proteins and the levels of liver inflammation factors were examined, the effect of PAL on microbiota was verified by FMT. The SER model revealed that PAL was the most important active ingredient in CS. 1H NMR fecal metabonomics showed that PAL could reserve the abnormal levels of gut microbial-mediated metabolites of liver fibrosis, such as isoleucine, taurine, butyrate, propionate, lactate, glucose, which mainly involved in amino acid metabolism, intestinal flora metabolism and energy metabolism. Metagenomics sequencing found that PAL could callback the abundance of s__Lactobacillus_murinus, s__Lactobacillus_reuteri, s__Lactobacillus_johnsonii, s__Lactobacillus_acidophilus and s__Faecalibaculum_rodentium to varying degree. Furthermore, the intestinal barrier function and the levels of hepatic inflammation factors were significantly ameliorated by PAL. FMT demonstrated that the therapeutic efficiency of PAL was closely associated with gut microbiota. The effects of CS on liver fibrosis were attributed in part to PAL by alleviating metabolic disorders and rebalancing gut microbiota. The SER strategy may be a useful method for the discovery of active constituents in natural plants. • SER strategy was used to identify the main active ingredients in CS. • PAL was revealed as the main component of CS against liver fibrosis. • PAL could improve CCl 4 -induced liver fibrosis by ameliorating metabolic disorder and rebalancing gut microbiota. [ABSTRACT FROM AUTHOR]

Breast cancer has been the most commonly-diagnosed cancer worldwide, and the treatment and prognosis of which are often limited by breast cancer stem cells (BCSCs). Litchi seeds have shown good anti-cancer activity in various cancers including prostate cancer, lung cancer and breast cancer. However, the activity and underlying mechanism of Litchi seeds against BCSCs remain unknown. To investigate the activity and mechanism of total flavonoids of litchi seed (TFLS) against BCSCs in vitro and in vivo. Two orthotopic xenograft mouse models were established using HCC1806 cells pretreated or untreated with TFLS to determine whether TFLS could target BCSCs in vivo. Mammosphere formation and flow cytometry assays were employed to evaluate the effect of TFLS on BCSCs in vitro. The underlying mechanism was investigated using RT-qPCR, Western blot, immunohistochemistry and immunofluorescence experiments. TFLS could significantly inhibit the viability of HCC1806, MCF-7 and HCC1937 cells in vitro and suppress the growth of HCC1806 cells in vivo. TFLS attenuated stem cell-like properties of breast cancer through reducing the percentage of CD44+CD24-/low cells, inhibiting the mammospheres formation and down-regulating the mRNA and protein levels of cancer stem cells related markers (Oct4, Nanog, Sox2) in MCF-7 and HCC1806 cells. Meanwhile, TFLS suppressed the tumor-initiating ability of BCSCs via reducing the percentage of CD44+CD24-/low cells in tumor and lowering tumor incidence rate in orthotopic xenograft mice. In addition, TFLS treatments restricted the expression and nuclear translocation of Notch3, subsequently down-regulated Hes1 and Runx2 expressions. TFLS could suppress the growth of breast cancer and eliminate breast cancer stem cells by inhibiting the Notch3 signaling pathway. [Display omitted] • TFLS inhibit breast cancer in vitro and in vivo. • TFLS attenuate breast cancer stem cells in vitro and restrict their tumorigenic ability in vivo. • TFLS decrease the expression and nuclear translocation of Notch3, subsequently down-regulate Hes1 and Runx2 expressions. [ABSTRACT FROM AUTHOR]

Diagnosis of rare skeletal diseases is based primarily on clinical phenotype and radiographic analysis. Genetic etiology of these heterogeneous diseases remains largely unknown. Here, we report the identification of two genomic mutations using exome sequencing from patients with multiple epiphyseal dysplasia (MED) of an unusual family in autosomal dominant and X-linked inheritance. A dominant mutation (c.2224G > A; p.Gly687Ser) in the known causal COL2A1 gene was identified in three patients with MED, deformed femoral heads and vertebral dysplasia. Furthermore, a hemizygous mutation (c.2830G > A; p.Ala944Thr) in the USP9X gene was identified in the fourth patient with short stature, MED, deformed femoral head, thoracic and lumbar platyspondyly, right ankle condyle dysplasia, and subchondral sclerosis. This is the first identification of an X-linked candidate causative gene in a patient with MED, suggesting a new clinical entity. Our findings shed a new light on the role of USP9X in MED-associated disorders. [ABSTRACT FROM AUTHOR]

Marsdenia tenacissima injection (MTE), a traditional Chinese medical injection extracted from the rattan of Marsdenia tenacissima (Roxb.) Moon, has been approved for clinical use in China as an adjuvant therapeutic agent in multiple cancers, including esophageal cancer, gastric cancer, lung cancer, and liver cancer. However, the activity and mechanism of MTE on prostate cancer (PCa) remain to be defined. To investigate the activity and the underlying mechanism of MTE in the treatment of PCa. The component characterization of MTE was analyzed by HPLC-CAD-QTOF-MS/MS technology. Cell Counting Kit-8 (CCK-8) assay was used to assess PCa cell proliferation. Colony formation assay was applied to detect the clonogenic ability of the cells. MetaboAnalyst5.0 database was employed to analyze the altered metabolites of PC3 cells treated with MTE obtained by UPLC-QTOF-MS/MS. Combined with metabolomics analysis and network pharmacology, we predicted the potential targets, which further were verified by Western Blot, RT-qPCR, and Immunohistochemistry assays. Finally, SeeSAR software was applied to predict the potential active components of MTE against PCa. A total of 21 components in MTE were confirmed by HPLC-CAD-QTOF-MS/MS analysis. MTE inhibited the proliferation and colony formation of PCa cells. A total of 20 metabolites closely related to glycerophospholipid metabolism, glycolysis/gluconeogenesis, and tricarboxylic acid (TCA) cycle were significantly changed in PC3 cells treated with MTE. The network pharmacology analysis revealed that MTE suppressed the growth of PC3 cells might by regulating the ErbB2-GSK3β-HIF1α signaling axis. Furthermore, we also confirmed that stimulation of MTE significantly inhibited the phosphorylation of ErbB2 at Tyr877 and the activities of its downstream signal transducers (GSK3β and HIF1α) in PCa, as well as the mRNA levels of critical factors (IDH2, LDHA, and HIF1A) in the tricarboxylic acid (TCA) cycle. Molecular docking further suggested that Tenacissimoside E, cryptochlorogenic acid, and scopoletin might be the active ingredients of MTE for PCa treatment. This study proposed that MTE exerts a potential anti-tumor effect in PCa through inhibiting ErbB2-GSK3β-HIF1α signaling axis, which may be related to the TCA cycle. [Display omitted] • MTE inhibits the proliferation of PCa cells. • ErbB2-GSK3β-HIF1α signaling axis is involved in the activity of MTE against PCa. • MTE alters the metabolism of PC3 cells by reducing the mRNA levels of IDH2 and LDHA. [ABSTRACT FROM AUTHOR]

To compare the clinical efficacy of the minimally invasive technique and the open method in the treatment of irreducible unilateral subaxial cervical facet joint dislocation (SCFD). From March 2015 to September 2018, 62 patients with unilateral SCFD were studied. The cases were divided into 2 groups based on different surgery strategies. Thirty-one patients were enrolled in the minimally invasive surgery (MIS) group, and 31 patients were enrolled in the open surgery group. The duration of prone position operation, blood loss, and total hospitalization costs were recorded. The clinical effects were evaluated using visual analogue scale scores, the Oswestry Disability Index, and Japanese Orthopedic Association scores at each follow-up. In addition, the segmental Cobb angle and intervertebral height were recorded and compared. The amount of intraoperative blood loss, prone position operation duration, and total hospital costs in the MIS group were significantly lower than in the open surgery group. The visual analogue scale, Oswestry Disability Index, and Japanese Orthopedic Association scores of the 2 groups significantly improved after the operation. A satisfactory fusion rate was obtained in both groups, and the segmental Cobb angle and intervertebral height scores in both groups improved significantly. Minimally invasive reduction had equal clinical efficacy to posterior open surgery. However, MIS was less invasive and had lower costs. Therefore, it is a potential option in the treatment of SCFD. [ABSTRACT FROM AUTHOR]

Background: As the increasing mortality and incidence of lung cancer (LC), there is an urgent need to discover novel treatment agent. In this study, we aimed to investigate the anti-LC effects of nitidine chloride (NC), a small molecular compound extracted from Chinese herbal medicine, while detailing its underlying mechanisms. Methods: Cell viability was detected by MTT assays and five cell death inhibitors, including ferrostatin-1 (Fer-1), Z-VAD-FMK, necrostatin-1 (Nec-1), disulfiram (DSF) and IM-54 were used to explore the type of cell death induced by NC. The microscopic features of NC-induced pyroptosis were assessed by transmission electron microscopy (TEM) and the pyroptotic-related proteins such as caspase and gasdermin family, were examined by western blot. Network pharmacology was employed to predict the potential mechanisms of NC in lung cancer treatment. CETSA and DARTs were used to determine the activity of NC binding to targeted protein. Xenograft mice model was established to further investigate the inhibitory effect and mechanism of NC against LC. Results: The pyroptosis inhibitor (DSF) and apoptosis inhibitor (Z-VAD-FMK) but not IM-54, necrostatin-1, or Ferrostatin-1 rescued NC-induced cell death. Morphologically, H1688 and A549 cells treated with NC showed notably pyroptotic features, such as cell swelling and large bubbles emerging from the plasma membrane. Gasdermin E (GSDME) rather than GSDMC or GSDMD was cleaved in NC-treated H1688 and A549 cells with an increased cleavage of caspase 3. Combined with network pharmacology and molecule docking, PI3K/Akt signaling axis was predicted and was further verified by CETSA and DARTs assay. In addition, the activation of PI3K is able to rescue the pyroptosis induced by NC in vitro. In xenograft model of LC, NC significantly hindered the transduction of PI3K-AKT pathway, inducing pyroptosis of tumor. Conclusion: Our data indicated that NC is a potential therapeutic agent for the treatment of LC via triggering GSDME-dependent pyroptosis. [ABSTRACT FROM AUTHOR]

This retrospective cross-sectional study was to investigate factors affecting clinical pregnancy in patients who received gonadotropin-releasing hormone agonist luteal phase long protocol (GnRH-a long protocol) and underwent fresh in-vitro fertilisation (IVF)/intracytoplasmic sperm injection (ICSI) embryo transfer cycle. One thousand five hundred and twenty-five patients who received GnRH-a long protocol and underwent fresh IVF/ICSI embryo transfer cycle were enrolled. The clinical pregnancy rate (63.1 vs. 22.4%, p <.05) and live birth rate (53.8 vs. 14.5%, p <.05) were significantly higher while the miscarriage rate (12.5 vs. 35.3%, p <.05) was significantly lower in the two embryo group than those in the one embryo group. The clinical pregnancy rate (48.5 vs. 64.1%, p <.05) and live birth rate (38.4 vs. 55.0%, p <.05) were significantly lower in patients older than 33.5 years than those in younger patients. The clinical pregnancy rate (52 and 60.6 vs. 79.7%, p <.05) and live birth rate (36 and 51.4 vs. 69.6%, p <.05) of the thin and mediate groups were significantly lower than those in the thick group, whereas the ectopic pregnancy rate (11.5 and 1.9 vs. 0%, p <.05) was significantly higher in the thin group than in the mediate and thick group. Multivariate logistic regression analysis showed that age (OR = 0.956, 95% CI [0.931, 0.982], p <.05), number of embryos transferred (OR = 2.491, 95% CI [1.670, 3.715], p <.05) and endometrial thickness on the transplantation day (OR = 1.124, 95% CI [1.067, 1.185], p <.05) were independent factors significantly associated with clinical pregnancy. In conclusion, endometrial thickness (>14.69 mm) on the day of transfer, two cleavage embryos transferred, and female age (≤33.5 years) are independent factors affecting clinical pregnancy outcomes in controlled ovarian hyperstimulation with GnRH-a long protocol for assisted conception. What is already known on this subject? Fresh embryo transfer cycle with GnRH-a long protocol will result in a higher pregnancy rate in controlled ovarian hyperstimulation cycles. What do the results of this study add? Endometrial thickness on the day of transfer, number of embryos transferred, and female age were independent factors affecting clinical pregnancy outcomes. What are the implications of these findings for clinical practice and/or further research? When performing a fresh IVF/ICSI embryo transfer cycle with GnRH-a long protocol for ovulation induction, the independent affecting factors should be taken into consideration. [ABSTRACT FROM AUTHOR]

Fufang Luohanguo Qingfei granules (LQG) is a Chinese patent medicine, clinically used to treat flu-like symptoms including cough with yellow phlegm, impeded phlegm, dry throat and tongue. However, the protective activity of LQG against influenza infection is indeterminate. This study is to investigate the therapeutic effect of LQG on influenza infection and elucidate its underlying mechanism. In vivo : A viral susceptible mouse model induced by restraint stress was established to investigate LQG's beneficial effects on influenza susceptibility. MAVS knockout (Mavs −/−) mice were used to verify the potential mechanism of LQG. In vitro : Corticosteroid (CORT)-treated A549 cells were employed to identify the active ingredients in LQG. Mice morbidity and mortality were monitored daily for 21 days. Histopathologic changes and inflammatory cytokines in lung tissues were examined by H&E staining and ELISA. RNA-seq was used to explore the signaling pathway influenced by LQG and further confirmed by qPCR. Immunoblotting and immunohistochemistry (IHC) were used to determine the protein levels. CO-IP and DARTS were applied to detect protein-protein interaction and compound-protein interaction, respectively. LQG effectively attenuated the susceptibility of restrained mice to H1N1 infection. LQG significantly boosted the production of IFN-β transduced by mitochondrial antiviral-signaling protein (MAVS), while MAVS deficiency abrogated its protective effects on restrained mice infected with H1N1. Moreover, in vitro studies further revealed that mogroside Ⅱ B, amygdalin, and luteolin are potentially active components of LQG. These results suggested that LQG inhibited the mitofusin 2 (Mfn2)-mediated ubiquitination of MAVS by impeding the E3 ligase synoviolin 1 (SYVN1) recruitment, thereby enhancing IFN-β antiviral response. Overall, our work elaborates a potential regimen for influenza treatment through reduction of stress-induced susceptibility. [Display omitted] • LQG reduces influenza virus susceptibility by improving MAVS/IFN-β pathway. • LQG curbs MAVS ubiquitination degradation by disrupting Mfn2/SYVN1 recruitment. • LQG's efficacy is abolished in Mavs−/− mice. • Mogroside Ⅱ B, amygdalin, and luteolin are potentially active components of LQG. [ABSTRACT FROM AUTHOR]

3A on p. 8312 showing the results from ALP staining and Alizarin Red S staining experiments, two pairs of apparently overlapping data panels were identified in the Dex 10 SP 6 sp M / TMP 50 M, 100 M and 200 M data panels. 2A on p. 8311, portraying the results of immunostaining experiments for osterix, the "GIOP" and "GIOP+TMP (20)" data panels contained overlapping data, such that these images were derived from apparently the same original source, where they were intended to show the results from differently performed experiments. Mol Med Rep 16: 8307-8314, 2017; DOI: 10.3892/mmr.2017.7610 Subsequently to the publication of the above paper, an interested reader drew to the authors' attention that, in Fig. [Extracted from the article]

Small-cell lung cancer (SCLC) is a highly metastatic and recalcitrant malignancy. Metastasis is the major cause of death in patients with SCLC but its mechanism remains poorly understood. An imbalance of hyaluronan catabolism in the extracellular matrix accelerates malignant progression in solid cancers due to the accumulation of low-molecular-weight HA. We previously found that CEMIP, a novel hyaluronidase, may act as a metastatic trigger in SCLC. In the present study, we found that both CEMIP and HA levels were higher in SCLC tissues than in paracancerous tissues from patient specimens and in vivo orthotopic models. Additionally, high expression of CEMIP was associated with lymphatic metastasis in patients with SCLC, and in vitro results showed that CEMIP expression was elevated in SCLC cells relative to human bronchial epithelial cells. Mechanistically, CEMIP facilitates the breakdown of HA and accumulation of LMW-HA. LMW-HA activates its receptor TLR2, and subsequently recruits c-Src to activate ERK1/2 signalling, thereby promoting F-actin rearrangement as well as migration and invasion of SCLC cells. In addition, the in vivo results verified that depletion of CEMIP attenuated HA levels and the expressions of TLR2, c-Src, and phosphorylation of ERK1/2, as well as liver and brain metastasis in SCLC xenografts. Furthermore, the application of the actin filament inhibitor latrunculin A significantly inhibited the liver and brain metastasis of SCLC in vivo. Collectively, our findings reveal the critical role of CEMIP-mediated HA degradation in SCLC metastasis and suggest its translational potential as an attractive target and a novel strategy for SCLC therapy. • Firstly revealing that CEMIP generates LMW-HA, leading to the accumulation of LMW-HA in SCLC ECM. • Indicating LMW-HA is an extracellular signal for promoting metastasis in SCLC. • Binding of LMW-HA and TLR2 induces F-actin rearrangement by activating c-Src/ERK1/2 axis. [ABSTRACT FROM AUTHOR]

[Display omitted] Small cell lung cancer (SCLC) is the most malignant lung cancer with rapid growth and early metastasis, but still lacks effective targeted therapies to improve the prognosis. Here, we demonstrated that a novel oncogenic protein, cell migration inducing hyaluronic binding protein (CEMIP), was robustly overexpressed in SCLC tissues than that in noncancerous tissues and high expression of CEMIP predicted poor outcomes in clinical specimens and in large sample size cohorts from public databases (GEPIA 2 and CPTAC). Liquid chromatography mass spectrometry (LC-MS) and in vitro/in vivo functional assays indicated that CEMIP contributed to the proliferation by increasing glutamine consumption and their metabolites (glutamate and glutathione) levels in SCLC cells. Moreover, the addition of a GLS1 inhibitor CB-839 dramatically reduced CEMIP-induced SCLC cell proliferation. Mechanistically, beyond as a scaffold protein, CEMIP facilitates glutamine-dependent cell proliferation through inhibiting c-Myc ubiquitination and increasing c-Myc stabilization and nuclear accumulation via hindering the interaction between FBXW7 (a E3 ubiquitin ligase) and its target substrate c-Myc. Taken together, our findings reveal a novel oncogenic role of CEMIP in sustaining SCLC growth via FBXW7/c-Myc-dependent axis, and provide new evidence that inhibition of CEMIP might be a potential therapeutic strategy for the treatment of SCLC. [ABSTRACT FROM AUTHOR]

Although silica aerogels with ultralow thermal conductivity are competitive for thermal insulation, their deficiencies in mechanical properties and single function affect their wide applications. Herein, we demonstrate a "soft fiber-stiff sheet" synergistic strategy for fabricating superelastic and responsive anisotropic silica nanofiber/polyvinylpyrrolidone/MXene hybrid aerogels by electrospinning, calcination, and directional-freezing. The anisotropic hybrid aerogel exhibits satisfactory thermal insulation performances at both high and low temperatures, delivering a low thermal conductivity of 21 mW m−1 K−1. The top surface temperature of the hybrid aerogel maintains at ∼74 °C after its standing on a hot plate of 300 °C. By putting the hybrid aerogel on a cold plate of −30 °C, its surface temperature is only 7.2 °C lower than the ambient temperature. Interestingly, the oxidation of MXene at high temperatures could transform the hybrid aerogel from electrically conductive to insulating, leading to sharp changes in electrical signals to trigger overheating alarm. Furthermore, the hybrid aerogel exhibits high compressibility and fatigue resistance with a high stress retention rate of 97.3% after 100 cycles under the compressive strain of 50%. Such compressive and thermally insulating aerogels are promising thermal management materials for smart alarms, and heat and cold protection of electronic devices. [Display omitted] [ABSTRACT FROM AUTHOR]