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Background: The sand fly Nyssomyia neivai is one of the most abundant species in Southern Brazil. It is frequently found in areas that are foci of visceral leishmaniasis in the state of Santa Catarina, caused by Leishmania infantum. In this region, the main vector of L. infantum, Lutzomyia longipalpis, has not been detected. In the absence of L. longipalpis, this study aimed to identify the sand fly fauna and diagnose any potential Leishmania spp. infection in sand flies and in dogs in a region of Southern Brazil that experienced a recent canine visceral leishmaniasis outbreak. Methods: This report includes a survey of the sand fly fauna at the Zoonosis Control Center of the Municipality of Tubarão (Santa Catarina, Brazil). Molecular tests were conducted to investigate Leishmania spp. natural infection in sand flies using polymerase chain reaction (PCR). In positive females, in addition to morphological identification, molecular analysis through DNA barcoding was performed to determine the sand fly species. Additionally, the dogs were tested for the presence of Leishmania spp. using a non-invasive technique for the collection of biological material, to be assessed by PCR. Results: A total of 3419 sand flies, belonging to five genera, were collected. Nyssomyia neivai was the most abundant species (85.8%), followed by Migonemyia migonei (13.3%), Pintomyia fischeri (0.8%), Evandromyia edwardsi (< 0.1%), and species of the genus Brumptomyia. (0.1%). Out of the 509 non-engorged females analyzed by PCR, two (0.4%) carried L. infantum DNA. The naturally infected females were identified as Ny. neivai, in both morphological and molecular analysis. In addition, two out of 47 conjunctival swabs from dogs tested positive for L. infantum, yielding an infection rate of 4.2%. Conclusions: These results confirm the presence of Ny. neivai naturally infected with L. infantum in an area where dogs were also infected by the parasite, suggesting its potential role as a vector in Southern Brazil. [ABSTRACT FROM AUTHOR]

Background: Understanding aspects related to the physiology and capacity of vectors is essential for effectively controlling vector-borne diseases. The sand fly Lutzomyia longipalpis has great importance in medical entomology for disseminating Leishmania parasites, the causative agent of Leishmaniasis, one of the main neglected diseases listed by the World Health Organization (WHO). In this respect, it is necessary to evaluate the transmission potential of this species and the success of vector control interventions. Near-infrared spectroscopy (NIRS) has been used to estimate the age of mosquitoes in different conditions (laboratory, semi-field, and conservation), taxonomic analysis, and infection detection. However, no studies are using NIRS for sand flies. Methods: In this study, we developed analytic models to estimate the age of L. longipalpis adults under laboratory conditions, identify their copulation state, and evaluate their gonotrophic cycle and diet. Results: Sand flies were classified with an accuracy of 58–82% in 3 age groups and 82–92% when separating them into young (<8 days) or old (>8 days) insects. The classification between mated and non-mated sandflies was 98–100% accurate, while the percentage of hits of females that had already passed the first gonotrophic cycle was only 59%. Conclusions: We consider the age and copula estimation results very promising, as they provide essential aspects of vector capacity assessment, which can be obtained quickly and at a lower cost with NIRS. Key message: Determining adult dipterans sand flies' age and mating status may be necessary for critical assessment of vector control interventions. Near-infrared spectroscopy (NIRS) is a fast, low-cost technique with many advantages compared to the traditional techniques that follow ovarian or cuticle development or pteridine content. In this work, we show for the first time to our knowledge that NIRS may be used for discrimination between young and old sand flies and mated or non-mated adults of both sexes in the Leishmania vector Lutzomyia longipalpis. [ABSTRACT FROM AUTHOR]

Background: The transmission dynamics of leishmaniasis are complex. There is also a lack of information about the ecological relationships between the vector/host/parasite at a more local and specific level. The Andean region concentrates more than 50% of Colombia's cutaneous leishmaniasis (CL) cases. The study of the ecological interactions of sand flies through the identification of blood sources has provided information on the female's opportunistic behavior, feeding on various hosts. Therefore, this study aimed to determine sand flies' ecological interactions with Leishmania parasites and their blood sources in an endemic area of CL. Results: A total of 4,621 sand flies were collected, comprising 20 species, in which the most abundant were Nyssomyia yuilli yuilli (55.4%), Psychodopygus ayrozai (14.5%) and Ps. panamensis (13.4%). Sequences of 12S gene fragment were analyzed using the BLASTn search tool. Blood-meal source identification was successfully performed for 47 sand flies, detecting seven vertebrate species, human and armadillo being the most frequent. Leishmania DNA was amplified in four female pools, constituted by Ny. yuilli yuilli and Ps. ayrozai, and the identification through RFLP detected Leishmania (Viannia) panamensis in the positive pools. Conclusions: The interactions between the sand fly species, local mammalian fauna and the Leishmania parasite in this active focus of CL, provide evidence of the potential role of two different species in the maintenance of the parasite transmission, important information for the understanding of the ecoepidemiology and transmission dynamics of the disease in Andean endemic areas. However its necessary further evaluations of the vector and host competence in the transmission and maintenance of Leishmania spp, in these complex and diverse areas. Author summary: The transmission dynamics of leishmaniasis are complex due to the variety of sand fly species, parasites and hosts involved. Although Colombia is considered an endemic country for the disease, the biology, ecology, and identification of blood-feeding sources of sand flies in some endemic areas remain unexplored. For this reason, this study aimed to analyze parameters related to the ecological interactions of the elements of the transmission cycle of cutaneous leishmaniasis. Interactions were detected through parasite and vertebrate DNA identification in sand flies collected intra- and peridomiciliary. Our findings show relevant information on distinct transmission patterns and the participation of different sand fly species in the maintenance of Leishmania panamensis in those environments, suggesting Nyssomyia yuilli yuilli as its primary vector and Psychodopygus ayrozai as a bridging species between wild and peridomiciliary cycle. [ABSTRACT FROM AUTHOR]

The incidence of vector-borne diseases is rising as deforestation, climate change, and globalization bring humans in contact with arthropods that can transmit pathogens. In particular, incidence of American Cutaneous Leishmaniasis (ACL), a disease caused by parasites transmitted by sandflies, is increasing as previously intact habitats are cleared for agriculture and urban areas, potentially bringing people into contact with vectors and reservoir hosts. Previous evidence has identified dozens of sandfly species that have been infected with and/or transmit Leishmania parasites. However, there is an incomplete understanding of which sandfly species transmit the parasite, complicating efforts to limit disease spread. Here, we apply machine learning models (boosted regression trees) to leverage biological and geographical traits of known sandfly vectors to predict potential vectors. Additionally, we generate trait profiles of confirmed vectors and identify important factors in transmission. Our model performed well with an average out of sample accuracy of 86%. The models predict that synanthropic sandflies living in areas with greater canopy height, less human modification, and within an optimal range of rainfall are more likely to be Leishmania vectors. We also observed that generalist sandflies that are able to inhabit many different ecoregions are more likely to transmit the parasites. Our results suggest that Psychodopygus amazonensis and Nyssomia antunesi are unidentified potential vectors, and should be the focus of sampling and research efforts. Overall, we found that our machine learning approach provides valuable information for Leishmania surveillance and management in an otherwise complex and data sparse system. Author summary: American Cutaneous Leishmaniasis (ACL) is a neglected disease caused by sandfly-transmitted parasites in the Americas. There is an incomplete understanding of which sandfly species transmit the parasite, complicating efforts to limit parasite transmission and consequently, disease burden. In this study, the authors created a database of sandfly traits, then used predictive models to determine important factors in parasite transmission and how different climate and environmental variables predict which vectors can transmit the parasites that cause ACL. The models suggest that transmission occurs at the interface between domestic habitats and well-preserved forests. The authors also generate predictions of which sandflies might be transmitting the parasites that are not known vectors at the time, specifically Psychodopygus amazonensis and Nyssomia antunesi. This new knowledge can lead to a better understanding of the system of transmission and can point to possible hotspots of risk. The analysis can also help direct researchers to areas of interest for sampling studies, as well as specific sandflies on which to focus effort. [ABSTRACT FROM AUTHOR]

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Mosquitoes (Diptera: Culicidae) from Porto Alegre region (Rio Grande do Sul state, Brazil) remain understudied. We therefore investigated mosquitoes occurring in 11 sites of this region, and evaluated which landscape features may explain the variation of species richness and composition of mosquito populations in different urban-natural areas of the study region. Using BG-Pro traps, we collected 4311 mosquitoes, from at least 50 species, 14 genera, and two subfamilies. Mansonia titillans (n = 598, 13.87%), Culex spp. (n = 513, 11.9%), and Mansonia wilsonii (n = 502, 11.65%) were the most abundant taxa. Aedes aegypti (n = 36, 0.84%) is potentially exploring new landscapes in the region, beyond human habitations. We identified two new descriptions for the Rio Grande do Sul state, Mansonia pessoai (n = 70, 1.62%) and Toxorhynchites theobaldi (n = 6, 0.14%). The model selection procedure identified that the percentage coverage of temporary crops and the Simpson's diversity index at landscape level as possible variables explaining the species richness, while percentage coverage of urban and temporary crops were the best predictors of species composition. BG-Pro traps were efficient for catching several adult mosquito species including Toxorhynchites mosquitoes. Therefore, this could be an interesting sampling methodology for mosquito surveillance. Implications for insect conservation: This study expanded the knowledge about Culicidae diversity in Brazil, highlighted the importance of green urban areas for biodiversity conservation, and provided data for entomological surveillance programs. This study also contributes to the conservation of mosquito species that pose no risk to human health (e.g., Toxorhynchites), highlighting that such species have relevant ecological roles, unrelated to disease transmission. [ABSTRACT FROM AUTHOR]

Mosquito blood feeding plays a key role in epidemiology. Despite its importance and large number of studies worldwide, less attention has been paid in South America. We summarized some general concepts and methodological issues related to the study of mosquito blood feeding habits, and compiled and analyzed all published information regarding the subject in the continent until 2020. Available literature comprised 152 scientific studies, that pursued different approaches: human landing catches (102 studies), baited trap (19), and blood meal analyses of collected specimens (38). Among the latter, 23 used serological and 15 molecular techniques. Species most frequently studied were those incriminated in malaria transmission, whereas relevant vectors such as Aedes aegypti, Ae. albopictus, and Haemagogus janthinomys were surprisingly neglected. Brazil was the leading country both in number of works and species studied. For over 70% of the species and three out of 13 South American countries there is no single information on mosquito blood feeding habits. Data from baited traps included 143 mosquito species, 83.9% of which were attracted to humans, either exclusively (10.5%) or in combination with other vertebrates (73.4%). Host blood identification of field collected specimens provided data on 102 mosquito species, and 60.8% of these fed on humans (55.9% combined with other vertebrates). Only 17 of the 73 species assessed by both methods yielded similar feeding patterns. Finally, supplementary tables are provided in a comprehensive summary of all information available and information gaps are highlighted for future research in the continent. [ABSTRACT FROM AUTHOR]

Introduction: Phlebotomine sand flies (Diptera: Psychodidae) are known as the vector of diseases such as leishmaniasis, bartonellosis and viral diseases. The aim of this study is to detect the host feeding pattern of sand flies in the endemic areas for leishmaniasis in Turkey (Antalya, Kayseri) and Northern Cyprus (TRNC) as well as the presence of Leishmania DNA in the specimens. Methods: One-hundred seventy-six blood-fed sand fly specimens were examined for blood meal analysis. A SYBR Green-PCR assay was performed with specific forward primers for each host and a universal reverse primer. Primers of human and goat were used together in multiplex PCR while goat and cow were studied separately. ITS-1 qPCR assay was also performed on both blood-fed and non-blood-fed females to detect Leishmania parasites. Results: Blood sources could be detected in 69 out of 176 blood-fed sand fly specimens. The results of blood meal analysis showed that specimens were fed mostly on cows (22.2%) followed by humans (5.7%), goats (2.8%) and dogs (0.6%). Multiple feeding patterns were also detected as human + cow (3.4%), cow + goat (2.8%) and human + goat (1.7%). Five of the blood-fed specimens were Leishmania spp. positive: P. major s.l. (n = 1), P. tobbi (n = 2) were L. tropica positive from Antalya, P. simici was positive for L. infantum from Kayseri and P. papatasi (n = 1) was positive for L. major from Cyprus. Leishmania infection rates were determined as 3.79%, 1.69% and 2.63% among the blood-fed sand fly specimens in Antalya, Kayseri and TRNC, respectively. Conclusion: The SYBR-Green-based multiplex PCR assay is a cost-effective and promising tool for blood meal identification of wild-caught sand flies as well as other blood-sucking arthropods. Feeding patterns of important vector species detected in the present study show the high risk in these endemic areas. As a next step, to identify the blood source in a shorter time and to make the test more sensitive, development of this assay to probe-based and multiplex PCR will be also planned. [ABSTRACT FROM AUTHOR]

Background: The 2017–2018 yellow fever virus (YFV) outbreak in southeastern Brazil marked a reemergence of YFV in urban states that had been YFV-free for nearly a century. Unlike earlier urban YFV transmission, this epidemic was driven by forest mosquitoes. The objective of this study was to evaluate environmental drivers of this outbreak. Methodology/Principal findings: Using surveillance data from the Brazilian Ministry of Health on human and non-human primate (NHP) cases of YFV, we traced the spatiotemporal progression of the outbreak. We then assessed the epidemic timing in relation to drought using a monthly Standardized Precipitation Evapotranspiration Index (SPEI) and evaluated demographic risk factors for rural or outdoor exposure amongst YFV cases. Finally, we developed a mechanistic framework to map the relationship between drought and YFV. Both human and NHP cases were first identified in a hot, dry, rural area in northern Minas Gerais before spreading southeast into the more cool, wet urban states. Outbreaks coincided with drought in all four southeastern states of Brazil and an extreme drought in Minas Gerais. Confirmed YFV cases had an increased odds of being male (OR 2.6; 95% CI 2.2–3.0), working age (OR: 1.8; 95% CI: 1.5–2.1), and reporting any recent travel (OR: 2.8; 95% CI: 2.3–3.3). Based on this data as well as mosquito and non-human primate biology, we created the "Mono-DrY" mechanistic framework showing how an unusual drought in this region could have amplified YFV transmission at the rural-urban interface and sparked the spread of this epidemic. Conclusions/Significance: The 2017–2018 YFV epidemic in Brazil originated in hot, dry rural areas of Minas Gerais before expanding south into urban centers. An unusually severe drought in this region may have created environmental pressures that sparked the reemergence of YFV in Brazil's southeastern cities. Author summary: In 2017–2018, cities in southeastern Brazil experienced an unusual outbreak of yellow fever virus. In the early 20th century, these cities had large outbreaks of yellow fever, spread by Aedes mosquitoes. But until this recent outbreak, they had been free of yellow fever for nearly a century. While this outbreak was spread by Haemagogus forest mosquitoes, the reemergence of yellow fever in densely populated urban areas raises serious concerns about it reestablishing ongoing transmission in cities, spread by urban Aedes mosquitoes. Our study sought to understand how and why yellow fever virus remerged in this area. We traced the outbreak, finding that it started in hot, dry, rural areas and spread south into cool, wet urban areas. The epidemic coincided with a severe drought, particularly in Minas Gerais where the epidemic started. Individuals with outdoor or rural risk factors were at highest risk, especially when the epidemic started. Therefore, this severe drought may have promoted the spread of yellow fever at rural-urban boundaries. To further explore this idea, we developed a unique framework based on forest mosquito and Howler monkey biology. Our framework, "Mono-DrY," shows how severe drought could have increased mosquito and monkey densities, promoting the spread of yellow fever. [ABSTRACT FROM AUTHOR]

Due to its public health importance, as vectors of Chagas disease, the subfamily Triatominae, has received the attention of taxonomists. Knowing how to correctly identify these insects is of paramount importance for the surveillance and control of these vectors. Over many years, more than 40 triatomine subspecies were proposed. Infraspecific taxa are accepted as real entities in nature and recognized as biologically significant. Infraspecific diversity and population subdivision are frequently confused with the underlying mechanisms that lead to the formation of new species and species-level diversity. The distinction between infraspecific and interspecific divergence processes has received far less attention than species delimitation. In this work, new literature data on subspecies included in the subfamily Triatominae are presented. Data were compiled about Triatoma brasiliensis, Triatoma breyeri, Triatoma circummaculata, Triatoma dimidiata, Triatoma incrassata, Triatoma infestans, Triatoma phyllosoma, Triatoma protracta, Triatoma recurva, Triatoma rubida, Triatoma sanguisuga, Triatoma vitticeps, Panstrongylus megistus, Paratriatoma hirsuta, and Paratriatoma lecticularia subspecies. Although several of these subspecies have been synonymized, herein, we emphasize that some subspecies may be valid. Therefore, further studies are needed on the taxonomy, evolution, phylogeny, biogeography, ecology, physiology, and behavior of species to reinforce, or to invalidate the infraspecific status in the subfamily. [ABSTRACT FROM AUTHOR]