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Cancer is the uncontrolled growth of new cells beyond the normal limits. The process by which cancers invade and spread to the other organs is called metastasis, this condition became the leading cause of death. One of detection methods that are currently used to detect tumors and metastatic tissues is conducted with radiopharmaceutical of [18F] fluoro-2-deoxy-2-D-glucose ([18F] FDG). This method is quite accurate, but is relatively expensive because it needs PET / CT camera that is still limited in Indonesia. Therefore, this study was conducted to develop other imaging methods using SPECT-CT camera that is cheaper and easy to reach especially for developing countries. Labelling 2-deoxy-D-glucose with technetium-99m (99mTc) indirectly using diethylenetriaminepentaacetic acid (DTPA) as a co-ligand / bifunctional agent has been carried out. The determination of the optimum conditions for the labelling of 99mTc-DTPA-DG were done by varying the amount of reducing agent (SnCl2.2H2O), ligand (2-deoxy-D-glucose), pH, and incubation time. Radiochemical purity of 99mTc-DTPA-DG was determined by ascending paper chromatography and electrophoresis paper. The results showed that the labelling optimum conditions of 99mTc-DTPA-DG was achieved by the number of ligand 2-deoxy-D-glucose as much as 2 mg, co-ligand DTPA 750 µg, 50 µg SnCl2.2H2O, the reaction takes place at pH 6 with an incubation time of 30 min at room temperature. 99mTc-DTPA-DG has a radiochemical purity of 93.16±1.31% and the electrophoresis results showed that there is a difference between the peaks of 99mTc-DTPA-DG with other impurities including 99mTc-DTPA. [ABSTRACT FROM AUTHOR]