13 results on '"Meléndez, Daniela M."'
Search Results
2. Auction market placement and a rest stop during transportation affect the respiratory bacterial microbiota of beef cattle
- Author
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Uddin, Muhammed Salah, primary, Schwartzkopf-Genswein, Karen S., additional, Waldner, Matthew, additional, Meléndez, Daniela M., additional, Niu, Yan D., additional, and Alexander, Trevor W., additional
- Published
- 2023
- Full Text
- View/download PDF
3. Auction market placement and a rest stop during transportation affect the respiratory bacterial microbiota of beef cattle.
- Author
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Salah Uddin, Muhammed, Schwartzkopf-Genswein, Karen S., Waldner, Matthew, Meléndez, Daniela M., Niu, Yan D., and Alexander, Trevor W.
- Subjects
HEALTH of cattle ,FEEDLOTS ,BEEF cattle ,ANIMAL welfare ,BEEF industry ,AUCTIONS ,CATTLE marketing - Abstract
Background: Bovine respiratory disease (BRD) is a significant health problem in beef cattle production, resulting in considerable economic losses due to mortalities, cost of treatment, and reduced feed efficiency. The onset of BRD is multifactorial, with numerous stressors being implicated, including transportation from farms to feedlots. In relation to animal welfare, regulations or practices may require mandatory rest times during transportation. Despite this, there is limited information on how transportation and rest stops affect the respiratory microbiota. Results: This study evaluated the effect of cattle source (ranch-direct or auction market-derived) and rest stop duration (0 or 8 h of rest) on the upper respiratory tract microbiota and its relationship to stress response indicators (blood cortisol and haptoglobin) of recently weaned cattle transported for 36 h. The community structure of bacteria was altered by feedlot placement. When cattle were off- loaded for a rest, several key bacterial genera associated with BRD (Mannheimia, Histophilus, Pasteurella) were increased for most sampling times after feedlot placement for the ranch-direct cattle group, compared to animals given no rest stop. Similarly, more sampling time points had elevated levels of BRD-associated genera when auction market cattle were compared to ranch-direct. When evaluated across time and treatments several genera including Mannheimia, Moraxella, Streptococcus and Corynebacterium were positively correlated with blood cortisol concentrations. Conclusion: This is the first study to assess the effect of rest during transportation and cattle source on the respiratory microbiota in weaned beef calves. The results suggest that rest stops and auction market placement may be risk factors for BRD, based solely on increased abundance of BRD-associated genera in the upper respiratory tract. However, it was not possible to link these microbiota to disease outcome, due to low incidence of BRD in the study populations. Larger scale studies are needed to further define how transportation variables impact cattle health. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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- View/download PDF
4. Effect of rest, post-rest transport duration, and conditioning on performance, behavioural, and physiological welfare indicators of beef calves
- Author
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Meléndez, Daniela M., primary, Marti, Sonia, additional, Haley, Derek B., additional, Schwinghamer, Timothy D., additional, Yang, Xiaohui, additional, and Schwartzkopf-Genswein, Karen S., additional
- Published
- 2022
- Full Text
- View/download PDF
5. 152 Effect of Continuous or Intermittent Feeding of Ergot Contaminated Grain in a Mash or Pelleted Form on the Performance of Backgrounding Beef Steers
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Reynolds, Matthew R, primary, Stanford, Kim, additional, Meléndez, Daniela M, additional, Schwartzkopf-Genswein, Karen S, additional, McAllister, Timothy A, additional, Blakley, Barry R, additional, McKinnon, John J, additional, and Ribeiro, Gabriel O, additional
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- 2022
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- View/download PDF
6. PSXIII-2 Effect of Rest, Post-Rest Transport Duration, and Conditioning on Performance and Physiological Welfare Indicators of Beef Calves
- Author
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Meléndez, Daniela M, primary, Marti, Sonia, additional, Schwinghamer, Timothy D, additional, Haley, Derek B, additional, Yang, Xiaohui, additional, Reynolds, Matthew R, additional, and Schwartzkopf-Genswein, Karen S, additional
- Published
- 2022
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7. Effects of Heating, Pelleting, and Feed Matrix on Apparent Concentrations of Cereal Ergot Alkaloids in Relation to Growth Performance and Welfare Parameters of Backgrounding Beef Steers
- Author
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Stanford, Kim, primary, Schwartzkopf-Genswein, Karen S., additional, Meléndez, Daniela M., additional, Ngo, Skyler, additional, Harding, Michael, additional, McAllister, Tim A., additional, Schatzmayr, Dian, additional, Swift, Mary Lou, additional, Blakley, Barry, additional, and Ribeiro, Gabriel O., additional
- Published
- 2022
- Full Text
- View/download PDF
8. Use of accelerometers to assess and describe trailer motion and its impact on carcass bruising in market cows transported under North American conditions
- Author
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Kehler, Carollyne E J, primary, Meléndez, Daniela M, additional, Ominski, Kim, additional, Crow, Gary, additional, Crowe, Trever G, additional, and Schwartzkopf-Genswein, Karen S, additional
- Published
- 2021
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9. Use of accelerometers to assess and describe trailer motion and its impact on carcass bruising in market cows transported under North American conditions.
- Author
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Kehler, Carollyne E J, Meléndez, Daniela M, Ominski, Kim, Crow, Gary, Crowe, Trever G, and Schwartzkopf-Genswein, Karen S
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TRAILERS ,DAIRY cattle ,ROOT-mean-squares ,COWS ,ANIMAL welfare ,ACCELEROMETERS ,TRANSPORT vehicles - Abstract
Increased trailer motion, coupled with large accelerations and decelerations, has been associated with decreased carcass quality and increased stress indicators in cattle, sheep, and hogs. However, motion of livestock trailers has not been measured in North-American cattle semi-trailers over long distances (> 1000 km). The objectives of this study were to develop a practical method of measuring transport trailer accelerations, to describe the range of accelerations cattle are exposed to under North American conditions, and to conduct a preliminary analysis of trailer accelerations for each compartment and its effect on carcass bruising. The root mean square (RMS) of acceleration was measured at a sampling rate of 200 Hz in 3 orthogonal axes; x (vertical), y (front-to-rear), and z (lateral; side-to-side) by clamping an accelerometer to the cross beam below each of the five compartments of 8 trailers transporting a total of 330 animals (674 ± 33.3 kg BW) from an assembly yard to a processing facility. Journeys took place on separate days and ranged in duration from 13 to 15.7 h. The number and severity of bruises per carcass were determined prior to trimming for n = 290 carcasses and the number of bruises per carcass ranged between 0.38 and 12.75, whereas the bruising score per carcass ranged between 0.38 and 14.88. Mean number of bruises and severity of bruises (bruising scores were assigned according to size using a three-point scale: 1) ≤ 6.5 cm, 2) 6.5 to 12 cm, and 3) ≥ 12 cm and bruising severity was determined by applying the weighted score to each bruise according to bruise area) per carcass was 4.52 ± 2.43 (n) and 5.31 ± 2.84, respectively. Accelerations in commercial transport vehicles were found to range between 0.33 and 1.90 m/s
2 , whereas the mean RMS of acceleration for all trailers (n = 31 accelerometers) was 1.01 ± 0.32 m/s2 , 0.72 ± 0.31 m/s2 , and 0.97 ± 0.30 m/s2 for the x, y, and z axes, respectively. Horizontal acceleration was greatest in the nose, back, and doghouse compartments (P = 0.05), whereas lateral acceleration was greatest in the nose and back compartments (P = 0.08). Although the nose, back, and doghouse compartments had the highest RMS values for the lateral and horizontal axes, there were no significant relationships between bruising and acceleration. Replication of this research is required to further understand the relationships between trailer motion, carcass bruising, and overall animal welfare in cattle transported long distances. [ABSTRACT FROM AUTHOR]- Published
- 2022
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- View/download PDF
10. Effect of Continuous Or Intermittent Feeding of Ergot Contaminated Grain in a Mash Or Pelleted Form on the Performance and Health Parameters of Finishing Beef Steers.
- Author
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Reynolds, Matthew R., Stanford, Kim, Meléndez, Daniela M., Schwartzkopf-Genswein, Karen S., McAllister, Tim A., Blakley, Barry R., McKinnon, John J., and Ribeiro, Gabriel O.
- Subjects
ERGOT alkaloids ,BLOOD cell count ,PELLETED feed ,BLOOD urea nitrogen ,ALANINE aminotransferase ,FUSARIUM toxins ,HAPTOGLOBINS - Abstract
This study evaluated the effect of feeding pelleted or mash ergot contaminated grain fed continuously or intermittently on the growth performance, blood serum variables, and carcass quality of finishing beef steers. Sixty black angus steers (442 ± 36.7 kg) were used in a complete randomized finishing (90% concentrate:10% barley silage DM basis) 126-d study. Steers were randomly assigned to 4 different treatments and individually housed. Treatments included: 1) control ration [CON; no added ergot alkaloids (EA)], 2) continuous ergot mash (CEM; fed continuously at 2 ppm total EA), 3) intermittent ergot mash (IEM; fed at 2 ppm EA during the first week of each 21-d period and control diet for the remaining 2 weeks), and 4) intermittent ergot pellet (IEP; fed at 2 ppm EA incorporated into a pelleted supplement during the first week of each 21-d period and control diet for the other 2 weeks). Steers were weighed every 21 d and blood samples were collected on day 0, 42, 84, and 126. Steer DMI (P < 0.01, 9.95 vs. 11.05 kg/day) and ADG (P = 0.04) decreased for all EA treatments compared with the CON. Total shrunk BW gain (P = 0.03, 202.5 vs. 225.2 kg), final BW (P = 0.03, 617.9 vs. 662.2 kg), and carcass weight (P = 0.06) decreased for all EA steers compared with the CON steers. No treatment effects (P > 0.05) were observed for carcass dressing percentage, ribeye area, marbling score, or liver abscess. The percent of carcasses graded AAA decreased for all EA steers compared CON steers (P < 0.01, 46.7 vs. 93.3%). Steers fed EA had increased rectal temperature compared with CON steers (P < 0.01, 39.8 vs. 39.4 °C). Hair cortisol concentrations were less in steers fed IEP (P = 0.05, 1.23 vs. 1.76 pg/mg) than CON. No treatment effects (P > 0.05) were observed for complete blood count, and serum prolactin, haptoglobin, albumin, alkaline phosphatase, alanine transaminase, amylase, calcium, creatine, glucose, lipase, total bilirubin, total protein, or globulin. Blood urea nitrogen was less for EA steers compared with CON (P = 0.01, 3.48 vs. 4.10 mmol/L). The IEM steers had decreased concentrations of serum gamma-glutamyl transferase compared with IEP steers (P = 0.04, 23.4 vs. 28.5 IU). Pelleting ergot contaminated grain did not reduce the impact of ergot alkaloids on any of the measured parameters. Results suggest that continuously or intermittently feeding an ergot contaminated diet (2 ppm EA) can significantly reduce intake, growth performance, and carcass weight, with minimal impact on blood serum variables in finishing steers. Pelleting was not an effective method of reducing ergot toxicity. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
11. Effect of continuous or intermittent feeding of ergot contaminated grain in a mash or pelleted form on the performance and health of feedlot beef steers
- Author
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Reynolds, Matthew R, Stanford, Kim, Meléndez, Daniela M, Schwartzkopf-Genswein, Karen S, McAllister, Tim A, Blakley, Barry R, McKinnon, John J, and Ribeiro, Gabriel O
- Abstract
This study evaluated the effect of feeding ergot contaminated grain continuously or intermittently through backgrounding (BG) and finishing (FN) in a mash or pelleted supplement on the growth performance, health and welfare parameters, and carcass characteristics of feedlot beef steers. Sixty black Angus steers (300 ± 29.4 kg BW) were used in a complete randomized 238-d study. Steers were stratified by weight and randomly assigned to four different diets (15 steers/treatment) and individually housed. Treatments included: (1) control [CON; no added ergot alkaloids (EA)], (2) continuous ergot mash (CEM; fed continuously at 2 mg total EA/kg of DM), (3) intermittent ergot mash (IEM; fed at 2 mg total EA/kg of DM, during the first week of each 21-d period and CON for the remaining 2 wk, this feeding pattern was repeated in each period), and (4) intermittent ergot pellet (IEP; fed at 2 mg of total EA/kg of DM as a pellet during the first week of each 21-d period and CON for the remaining 2 wk as described for IEM). Steers were fed barley based BG diets containing 40% concentrate:60% silage (DM basis) for 84 d (four 21-d periods), transitioned over 28 d (no ergot fed) to an FN diet (90% concentrate:10% silage DM basis) and fed for 126 d (six 21-d periods) before slaughter. In the BG phase, steer DMI (P< 0.01, 7.45 vs. 8.05 kg/d) and ADG (P< 0.01) were reduced for all EA diets compared to CON. The CEM fed steers had lower ADG (P< 0.01, 0.735 vs. 0.980 kg) and shrunk final BW (P< 0.01, 350 vs. 366 kg) than CON. CEM had lower gain:feed (P< 0.07, 0.130 vs. 0.142) than CON. In the FN phase, steer DMI (P< 0.01, 9.95 vs. 11.05 kg/d) and ADG (P= 0.04) were also decreased for all EA fed steers compared to CON. Total shrunk BW gain (P = 0.03, 202.5 vs. 225.2 kg), final BW (P= 0.03, 617.9 vs. 662.2 kg), and carcass weight (P= 0.06) decreased for all EA fed steers compared to CON. The percentage of AAA carcasses decreased for all EA fed steers (P< 0.01, 46.7 vs. 93.3%) compared to CON. EA fed steers had increased rectal temperatures (P< 0.01, 39.8 vs. 39.4 °C) compared to CON. Pelleting ergot contaminated grain did not reduce the impact of ergot alkaloids on any of the measured parameters during BG or FN. Continuously or intermittently feeding ergot contaminated diets (2 mg total EA/kg of DM) significantly reduced intake, growth performance, and carcass weight, with minimal impact on blood parameters in feedlot steers. Pelleting was not an effective method of reducing ergot toxicity.This study evaluated the effect of continuous or intermittent feeding EA in a mash or pelleted form on growth performance, health and welfare parameters, and carcass characteristics of feedlot beef steers. Continuously or intermittently feeding an ergot contaminated diet (2 ppm EA) both reduced intake, growth performance, and carcass weight, but pelleting did not reduce the toxicities of EA.Produced by the fungus Claviceps purpurea, ergot alkaloids (EA) are toxic to beef cattle when consumed and can lead to reduction in feed intake and growth performance, vasoconstriction of the blood vessels, hyperthermia, damage to extremities (ears, tails, and hooves) and in severe cases, death. Grain is often cleaned to meet quality standards, and the resulting screenings are often utilized for feeding livestock and can have high concentrations of EA. The application of heat during pelleting of EA contaminated grain has been suggested to reduce its toxicity. Backgrounding and finishing beef cattle feeding experiments were conducted to assess the effect of continuously or intermittently feeding EA contaminated grain (2 mg/kg of diet DM) either as a pellet or as mash on growth performance, health, and animal welfare. Feeding EA grain continuously or intermittently either as a mash or pellet drastically reduced growth performance of steers, with no difference between treatments.
- Published
- 2024
- Full Text
- View/download PDF
12. Effect of Rest, Post-Rest Transport Duration, and Conditioning on Performance and Physiological Welfare Indicators of Beef Calves.
- Author
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Meléndez, Daniela M., Marti, Sonia, Schwinghamer, Timothy D., Haley, Derek B., Xiaohui Yang, Reynolds, Matthew R., and Schwartzkopf-Genswein, Karen S.
- Subjects
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CALVES , *CREATINE kinase , *FACTORIAL experiment designs , *REST periods , *FATTY acids , *HAPTOGLOBINS - Abstract
The aim of this study was to assess the effects of conditioning, rest, and post-rest transport duration on welfare indicators of 6-7 mo old beef calves. A total of 328 weaned calves (237 ± 29.7 kg BW) were randomly assigned to a 2 × 2 × 2 factorial design: conditioning, conditioned (C) or non-conditioned (N); rest, 0 (R0) or 8 (R8) h, and post-rest transport, 4 (T4) or 15 (T15) h. Calves were sampled prior to and after 20h of transport, prior to and after the additional 4 or 15-h transport, and at 1, 2, 3, 5, 14, and 28 d after transport ended. Data were analyzed using the GLIMMIX procedure of SAS. Fixed effects were conditioning, transport and time nested within rest period, while random effects were animal and pen. For R0-T4 calves, the mean L-lactate concentrations were greater than R8-T4 calves on d 1 and 2 (p-values = 0.02) while, for R0 calves, mean ADG was greater than R8 calves 14 to 28 d after transport (p-values < 0.01). For R8-T4 calves 1 week after transport, mean ADG was greater and mean WBC counts were less than R8-T15 calves on d 5 (p-values < 0.01). For N calves, overall mean haptoglobin, creatine kinase, serum amyloid-A, and non-esterified fatty acids were greater than C calves (p-values = 0.05). Overall, few and inconsistent differences were observed for rest, where rest improved L-lactate but affected ADG. Few differences were observed for transport, where shorter transport durations after the rest improved ADG and WBC counts. In general, N calves had greater physiological indicators of reduced welfare than C calves. Based on these findings, the best way to improve calf welfare during and after transport is to condition them prior to transport. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
13. Effect of Continuous or Intermittent Feeding of Ergot Contaminated Grain in a Mash or Pelleted Form on the Performance of Backgrounding Beef Steers.
- Author
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Reynolds, Matthew R., Stanford, Kim, Meléndez, Daniela M., Schwartzkopf-Genswein, Karen S., McAllister, Timothy A., Blakley, Barry R., McKinnon, John J., and Ribeiro, Gabriel O.
- Subjects
CALVES ,GRAIN ,BLOOD cell count ,ERGOT alkaloids ,FEEDLOTS ,PELLETED feed - Abstract
The objective of this study was to evaluate the effect of feeding pelleted or mash ergot contaminated grain continuously or intermittently on performance and blood parameters of backgrounding steers. Sixty black angus steers (300 ± 29.4 kg) were used in a complete randomized backgrounding (60% barley silage:40% concentrate DM basis) 84 d study. Steers were randomly assigned to 4 different treatments and housed individually. Treatments included: 1) control ration (CON; no added ergot), 2) continuous ergot mash (CEM; fed continuously at 2 ppm), 3) intermittent ergot mash (IEM; fed at 2 ppm on the first wk of each 21 d period and control diet for the remaining 2 wk), and 4) intermittent ergot pellet (IEP; fed at 2 ppm during the first wk of each 21 d period and control diet for the other 2 wk). Calves were weighed every 21 days and blood samples were collected on d 0, 42, and 84. No treatment effects (P>0.05) were observed for complete blood count. Steer DMI was reduced for all ergot diets compared to the CON diet (P< 0.01, 7.45 vs. 8.05 kg/d). Steer ADG was reduced for all ergot diets compared to the CON diet (P< 0.01). CEM fed steers had lower ADG (P< 0.01, 0.980 vs. 0.735 kg) and shrunk final weight (P< 0.01, 366 vs. 350 kg) than CON calves, but CEM and IEM were no different. CEM fed steers had lower Gain:Feed (P< 0.07, 0.130 vs. 0.142) than CON calves. Pelleting ergot contaminated grain did not reduce the impact of ergot alkaloids on any of the measured parameters. Results suggest that continuously or intermittently feeding an ergot contaminated TMR (2 ppm) can significantly reduce the growth performance of backgrounded feedlot steers but no effects were observed for blood parameters. Pelleting was not an effective method of reducing ergot toxicity. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
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