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Your search keyword '"Melissa M. Kemp"' showing total 10 results
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10 results on '"Melissa M. Kemp"'

1. Supplemental Figure 3 from CUE-101, a Novel E7-pHLA-IL2-Fc Fusion Protein, Enhances Tumor Antigen-Specific T-Cell Activation for the Treatment of HPV16-Driven Malignancies

Author

Mary Ellen Simcox, Kenneth J. Pienta, Saso Cemerski, Anish Suri, Ronald Seidel, Rodolfo Chaparro, Steven Almo, Peter A. Kiener, John F. Ross, Emily Spaulding, Mark Haydock, Jonathan Soriano, Jessica Ryabin, Luke Witt, Dominic R. Beal, Lauren D. Kraemer, Alyssa Nelson, Sandrine Hulot, Paige Ruthardt, Miguel Moreta, Fan Zhao, Alex Histed, Melissa M. Kemp, Zohra Merazga, Dharma R. Thapa, Natasha Girgis, and Steven N. Quayle

Abstract

Supplementary Figure 3. Representative gating strategy for the analysis of blood and spleen samples from HLA-A2 transgenic mice.

Published
2023

2. Supplemental Figure 5 from CUE-101, a Novel E7-pHLA-IL2-Fc Fusion Protein, Enhances Tumor Antigen-Specific T-Cell Activation for the Treatment of HPV16-Driven Malignancies

Author

Mary Ellen Simcox, Kenneth J. Pienta, Saso Cemerski, Anish Suri, Ronald Seidel, Rodolfo Chaparro, Steven Almo, Peter A. Kiener, John F. Ross, Emily Spaulding, Mark Haydock, Jonathan Soriano, Jessica Ryabin, Luke Witt, Dominic R. Beal, Lauren D. Kraemer, Alyssa Nelson, Sandrine Hulot, Paige Ruthardt, Miguel Moreta, Fan Zhao, Alex Histed, Melissa M. Kemp, Zohra Merazga, Dharma R. Thapa, Natasha Girgis, and Steven N. Quayle

Abstract

Supplementary Figure 5. Antitumor activity of mCUE-101 in TC-1 tumors.

Published
2023

3. Data from CUE-101, a Novel E7-pHLA-IL2-Fc Fusion Protein, Enhances Tumor Antigen-Specific T-Cell Activation for the Treatment of HPV16-Driven Malignancies

Author

Mary Ellen Simcox, Kenneth J. Pienta, Saso Cemerski, Anish Suri, Ronald Seidel, Rodolfo Chaparro, Steven Almo, Peter A. Kiener, John F. Ross, Emily Spaulding, Mark Haydock, Jonathan Soriano, Jessica Ryabin, Luke Witt, Dominic R. Beal, Lauren D. Kraemer, Alyssa Nelson, Sandrine Hulot, Paige Ruthardt, Miguel Moreta, Fan Zhao, Alex Histed, Melissa M. Kemp, Zohra Merazga, Dharma R. Thapa, Natasha Girgis, and Steven N. Quayle

Abstract

Purpose:To assess the potential for CUE-101, a novel therapeutic fusion protein, to selectively activate and expand HPV16 E711-20-specific CD8+ T cells as an off-the shelf therapy for the treatment of HPV16-driven tumors, including head and neck squamous cell carcinoma (HNSCC), cervical, and anal cancers.Experimental Design:CUE-101 is an Fc fusion protein composed of a human leukocyte antigen (HLA) complex, an HPV16 E7 peptide epitope, reduced affinity human IL2 molecules, and an effector attenuated human IgG1 Fc domain. Human E7-specific T cells and human peripheral blood mononuclear cells (PBMC) were tested to demonstrate cellular activity and specificity of CUE-101, whereas in vivo activity of CUE-101 was assessed in HLA-A2 transgenic mice. Antitumor efficacy with a murine surrogate (mCUE-101) was tested in the TC-1 syngeneic tumor model.Results:CUE-101 demonstrates selective binding, activation, and expansion of HPV16 E711-20-specific CD8+ T cells from PBMCs relative to nontarget cells. Intravenous administration of CUE-101 induced selective expansion of HPV16 E711-20-specific CD8+ T cells in HLA-A2 (AAD) transgenic mice, and anticancer efficacy and immunologic memory was demonstrated in TC-1 tumor-bearing mice treated with mCUE-101. Combination therapy with anti-PD-1 checkpoint blockade further enhanced the observed efficacy.Conclusions:Consistent with its design, CUE-101 demonstrates selective expansion of an HPV16 E711-20-specific population of cytotoxic CD8+ T cells, a favorable safety profile, and in vitro and in vivo evidence supporting its potential for clinical efficacy in an ongoing phase I trial (NCT03978689).

Published
2023

4. Supplementary Materials and Methods from CUE-101, a Novel E7-pHLA-IL2-Fc Fusion Protein, Enhances Tumor Antigen-Specific T-Cell Activation for the Treatment of HPV16-Driven Malignancies

Author

Mary Ellen Simcox, Kenneth J. Pienta, Saso Cemerski, Anish Suri, Ronald Seidel, Rodolfo Chaparro, Steven Almo, Peter A. Kiener, John F. Ross, Emily Spaulding, Mark Haydock, Jonathan Soriano, Jessica Ryabin, Luke Witt, Dominic R. Beal, Lauren D. Kraemer, Alyssa Nelson, Sandrine Hulot, Paige Ruthardt, Miguel Moreta, Fan Zhao, Alex Histed, Melissa M. Kemp, Zohra Merazga, Dharma R. Thapa, Natasha Girgis, and Steven N. Quayle

Abstract

Supplementary Materials and Methods

Published
2023

5. Supplemental Figure 4 from CUE-101, a Novel E7-pHLA-IL2-Fc Fusion Protein, Enhances Tumor Antigen-Specific T-Cell Activation for the Treatment of HPV16-Driven Malignancies

Author

Mary Ellen Simcox, Kenneth J. Pienta, Saso Cemerski, Anish Suri, Ronald Seidel, Rodolfo Chaparro, Steven Almo, Peter A. Kiener, John F. Ross, Emily Spaulding, Mark Haydock, Jonathan Soriano, Jessica Ryabin, Luke Witt, Dominic R. Beal, Lauren D. Kraemer, Alyssa Nelson, Sandrine Hulot, Paige Ruthardt, Miguel Moreta, Fan Zhao, Alex Histed, Melissa M. Kemp, Zohra Merazga, Dharma R. Thapa, Natasha Girgis, and Steven N. Quayle

Abstract

Supplementary Figure 4. Antitumor activity of mCUE-101 in established TC-1 tumors.

Published
2023

6. Supplemental Figure 6 from CUE-101, a Novel E7-pHLA-IL2-Fc Fusion Protein, Enhances Tumor Antigen-Specific T-Cell Activation for the Treatment of HPV16-Driven Malignancies

Author

Mary Ellen Simcox, Kenneth J. Pienta, Saso Cemerski, Anish Suri, Ronald Seidel, Rodolfo Chaparro, Steven Almo, Peter A. Kiener, John F. Ross, Emily Spaulding, Mark Haydock, Jonathan Soriano, Jessica Ryabin, Luke Witt, Dominic R. Beal, Lauren D. Kraemer, Alyssa Nelson, Sandrine Hulot, Paige Ruthardt, Miguel Moreta, Fan Zhao, Alex Histed, Melissa M. Kemp, Zohra Merazga, Dharma R. Thapa, Natasha Girgis, and Steven N. Quayle

Abstract

Supplementary Figure 6. Characterization of E7-specific CD8+ T cells in mice bearing TC-1 tumors.

Published
2023

7. Supplementary Table from CUE-101, a Novel E7-pHLA-IL2-Fc Fusion Protein, Enhances Tumor Antigen-Specific T-Cell Activation for the Treatment of HPV16-Driven Malignancies

Author

Mary Ellen Simcox, Kenneth J. Pienta, Saso Cemerski, Anish Suri, Ronald Seidel, Rodolfo Chaparro, Steven Almo, Peter A. Kiener, John F. Ross, Emily Spaulding, Mark Haydock, Jonathan Soriano, Jessica Ryabin, Luke Witt, Dominic R. Beal, Lauren D. Kraemer, Alyssa Nelson, Sandrine Hulot, Paige Ruthardt, Miguel Moreta, Fan Zhao, Alex Histed, Melissa M. Kemp, Zohra Merazga, Dharma R. Thapa, Natasha Girgis, and Steven N. Quayle

Abstract

Supplementary Table 1

Published
2023

8. Supplemental Figure 1 from CUE-101, a Novel E7-pHLA-IL2-Fc Fusion Protein, Enhances Tumor Antigen-Specific T-Cell Activation for the Treatment of HPV16-Driven Malignancies

Author

Mary Ellen Simcox, Kenneth J. Pienta, Saso Cemerski, Anish Suri, Ronald Seidel, Rodolfo Chaparro, Steven Almo, Peter A. Kiener, John F. Ross, Emily Spaulding, Mark Haydock, Jonathan Soriano, Jessica Ryabin, Luke Witt, Dominic R. Beal, Lauren D. Kraemer, Alyssa Nelson, Sandrine Hulot, Paige Ruthardt, Miguel Moreta, Fan Zhao, Alex Histed, Melissa M. Kemp, Zohra Merazga, Dharma R. Thapa, Natasha Girgis, and Steven N. Quayle

Abstract

Supplementary Figure 1. Characterization of CUE-101 binding properties.

Published
2023

9. Colonial Legacies Influence Biodiversity Lessons: How Past Trade Routes and Power Dynamics Shape Present-Day Scientific Research and Professional Opportunities for Caribbean Scientists.

Author

Mohammed RS, Turner G, Fowler K, Pateman M, Nieves-Colón MA, Fanovich L, Cooke SB, Dávalos LM, Fitzpatrick SM, Giovas CM, Stokowski M, Wrean AA, Kemp M, LeFebvre MJ, and Mychajliw AM

Subjects
Biological Evolution, Caribbean Region, Humans, Biodiversity, Ecosystem
Abstract

AbstractScientists recognize the Caribbean archipelago as a biodiversity hotspot and employ it for their research as a natural laboratory. Yet they do not always appreciate that these ecosystems are in fact palimpsests shaped by multiple human cultures over millennia. Although post-European anthropogenic impacts are well documented, human influx into the region began about 5,000 years prior. Thus, inferences of ecological and evolutionary processes within the Caribbean may in fact represent artifacts of an unrecognized human legacy linked to issues influenced by centuries of colonial rule. The threats posed by stochastic natural and anthropogenically influenced disasters demand that we have an understanding of the natural history of endemic species if we are to halt extinctions and maintain access to traditional livelihoods. However, systematic issues have significantly biased our biological knowledge of the Caribbean. We discuss two case studies of the Caribbean's fragmented natural history collections and the effects of differing governance by the region's multiple nation states. We identify knowledge gaps and highlight a dire need for integrated and accessible inventorying of the Caribbean's collections. Research emphasizing local and international collaboration can lead to positive steps forward and will ultimately help us more accurately study Caribbean biodiversity and the ecological and evolutionary processes that generated it.

Published
2022
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10. Annotation of putative circadian rhythm-associated genes in Diaphorina citri (Hemiptera: Liviidae).

Author

Reynolds M, de Oliveira L, Vosburg C, Paris T, Massimino C, Norus J, Ortiz Y, Espino M, Davis N, Masse R, Neiman A, Holcomb R, Gervais K, Kemp M, Hoang M, Shippy TD, Hosmani PS, Flores-Gonzalez M, Pelz-Stelinski K, Qureshi JA, Mueller LA, Hunter WB, Benoit JB, Brown SJ, D'Elia T, and Saha S

Abstract

The circadian rhythm involves multiple genes that generate an internal molecular clock, allowing organisms to anticipate environmental conditions produced by the Earth's rotation on its axis. Here, we present the results of the manual curation of 27 genes that are associated with circadian rhythm in the genome of Diaphorina citri, the Asian citrus psyllid. This insect is the vector for the bacterial pathogen Candidatus Liberibacter asiaticus ( C Las), the causal agent of citrus greening disease (Huanglongbing). This disease severely affects citrus industries and has drastically decreased crop yields worldwide. Based on cry1 and cry2 identified in the psyllid genome, D. citri likely possesses a circadian model similar to the lepidopteran butterfly, Danaus plexippus . Manual annotation will improve the quality of circadian rhythm gene models, allowing the future development of molecular therapeutics, such as RNA interference or antisense technologies, to target these genes to disrupt the psyllid biology., Competing Interests: The authors declare that they have no competing interests., (© The Author(s) 2022.)

Published
2022
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