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Failure is hard‐wired into the scientific method and yet teaching students to productively engage with failure is not foundational in most biology curricula. To train successful scientists, it is imperative that we teach undergraduate science students to be less fearful of failure and to instead positively accept it as a productive part of the scientific process. In this article, we focus on student perceptions of the stigma of failure and their associated concerns to explore how failure could be better supported within and beyond a university context. Through a survey of first‐year biology students, we found that societal and familial pressures to succeed were the greatest contributing factors to students' fear of failure. In student suggestions on how to reduce the stigma of failure within and beyond the university context, the most common theme identified across both contexts was for increased discussion and open communication about experiences of failure. Importantly, student comments in this study bring attention to the role of factors beyond the classroom in shaping student experiences of failure within their biology courses.

Non-excitable cells express sodium voltage-gated channel alpha subunit 1 gene and protein (known as SCN1A and NaV 1.1, respectively); however, the functions of NaV 1.1 are unclear. In this study, we investigated the role of SCN1A and NaV 1.1 in human mesenchymal stem cells (MSCs). We found that SCN1A was expressed in MSCs, and abundant expression of NaV 1.1 was observed in the endoplasmic reticulum; however, this expression was not found to be related to Na+ currents. SCN1A-silencing reduced MSC proliferation and delayed the cell cycle in the S phase. SCN1A silencing also suppressed the protein levels of CDK2 and AKT (herein referring to total AKT), despite similar mRNA expression, and inhibited AKT phosphorylation in MSCs. A cycloheximide-chase assay showed that SCN1A-silencing induced CDK2 but not AKT protein degradation in MSCs. A proteolysis inhibition assay using epoxomicin, bafilomycin A1 and NH4 Cl revealed that both the ubiquitin–proteasome system and the autophagy and endo- lysosome system were irrelevant to CDK2 and AKT protein reduction in SCN1A-silenced MSCs. The AKT inhibitor LY294002 did not affect the degradation and nuclear localization of CDK2 in MSCs. Likewise, the AKT activator SC79 did not attenuate the SCN1A-silencing effects on CDK2 in MSCs. These results suggest that NaV 1.1 contributes to the cell cycle of MSCs by regulating the post-translational control of AKT and CDK2. [ABSTRACT FROM AUTHOR]

Purpose: Kono-S anastomosis, an antimesenteric, functional, end-to-end handsewn anastomosis, was introduced in 2011. The aim of this meta-analysis is to evaluate the safety and effectivity of the Kono-S technique. Methods: A comprehensive search of MEDLINE (PubMed), Embase (Elsevier), Scopus (Elsevier), and Cochrane Central (Ovid) from inception to August 24th, 2023, was conducted. Studies reporting outcomes of adults with Crohn's disease undergoing ileocolic resection with subsequent Kono-S anastomosis were included. PRISMA and Cochrane guidelines were used to screen, extract and synthesize data. Primary outcomes assessed were endoscopic, surgical and clinical recurrence rates, as well as complication rates. Data were pooled using random-effects models, and heterogeneity was assessed with I² statistics. ROBINS-I and ROB2 tools were used for quality assessment. Results: 12 studies involving 820 patients met the eligibility criteria. A pooled mean follow-up time of 22.8 months (95% CI: 15.8, 29.9; I2 = 99.8%) was completed in 98.3% of patients. Pooled endoscopic recurrence was reported in 24.1% of patients (95% CI: 9.4, 49.3; I2 = 93.43%), pooled surgical recurrence in 3.9% of patients (95% CI: 2.2, 6.9; I2 = 25.97%), and pooled clinical recurrence in 26.8% of patients (95% CI: 14, 45.1; I2 = 84.87%). The pooled complication rate was 33.7%. The most common complications were infection (11.5%) and ileus (10.9%). Pooled anastomosis leakage rate was 2.9%. Conclusions: Despite limited and heterogenous data, patients undergoing Kono-S anastomosis had low rates of surgical recurrence and anastomotic leakage with moderate rates of endoscopic recurrence, clinical recurrence and complications rate. [ABSTRACT FROM AUTHOR]

Failure is hard‐wired into the scientific method and yet teaching students to productively engage with failure is not foundational in most biology curricula. To train successful scientists, it is imperative that we teach undergraduate science students to be less fearful of failure and to instead positively accept it as a productive part of the scientific process. In this article, we focus on student perceptions of the stigma of failure and their associated concerns to explore how failure could be better supported within and beyond a university context. Through a survey of first‐year biology students, we found that societal and familial pressures to succeed were the greatest contributing factors to students' fear of failure. In student suggestions on how to reduce the stigma of failure within and beyond the university context, the most common theme identified across both contexts was for increased discussion and open communication about experiences of failure. Importantly, student comments in this study bring attention to the role of factors beyond the classroom in shaping student experiences of failure within their biology courses. [ABSTRACT FROM AUTHOR]

The aim of this study is the elaboration of super-hydrophobicpolyester fabrics (PET) coated organophilic cellulose nanocrystals (CNC-ODA) using dip-coating approach. The CNC-ODA was successfully synthesized from microcrystalline cellulose via a chemical oxidation method, followed by carboxylation and chemical grafting of long chain fatty amine octadecylamine via an amidation reaction. PET fabrics loaded with different CNC-ODA contents, ranging from 1 to 5% wt. were successfully prepared and characterized by several techniques. FTIR confirmed the formation of interfacial interaction between the PET and CNC-ODA functional groups. The contact angle measurements revealed that treated PET fabrics displayed super-hydrophobic character with a contact angle of 153.1°. Tensile strength test revealed that the produced fabrics exhibited improved mechanical properties compared to pristine PET. Moreover, as a versatile application, the elaborated PET@CNC-ODA textile displayed excellent oil/water separation for different organic compounds/water mixtures. The obtained results are very motivating, in term of elaborating durable super-hydrophobic textiles.

Herein, we report the design of a cost-effective catalyst with excellent recyclability, simple recuperation and facile recovery, and the examination between the reaction cycles via the development of self-supporting g-C3N4 nanosheets/Ag NPs polyester fabric (PES) using a simple, facile and efficient approach. PES fabrics were coated via a sono-coating method with carbon nitride nanosheets (GCNN) along with an in situ setting of Ag nanoparticles on PES coated GCNN surface producing PES-GCNN/Ag0. The elaborated textile-based materials were fully characterized using FTIR, 13C NMR, XRD, TGA, SEM, EDX, etc. Catalytic performance of the designed “Dip-Catalyst” demonstrated that the as-prepared PES-GCCN/Ag0 has effectively catalyzed the hydrogenation of 4-nitrophenol (4-NP) to 4-aminophenol (4-AP) in the presence of NaBH4. The 3 × 3 cm2 PES-GCNN/Ag0 showed the best catalytic activity, displaying an apparent rate constant (Kapp) equal to 0.43 min−1 and more than 10 reusability cycles, suggesting that the prepared catalyst-based PES fabric can be a strong nominee for sustainable chemical catalysis. Moreover, the coated fabrics exhibited appreciable antibacterial capacity against Staphylococcus epidermidis (S. epidermidis) and Escherichia coli (E. coli). The present study opens up new opportunities for the future design of a low cost and large-scale process of functional fabrics.

Non-excitable cells express sodium voltage-gated channel alpha subunit 1 gene and protein (known as SCN1A and NaV1.1, respectively); however, the functions of NaV1.1 are unclear. In this study, we investigated the role of SCN1A and NaV1.1 in human mesenchymal stem cells (MSCs). We found that SCN1A was expressed in MSCs, and abundant expression of NaV1.1 was observed in the endoplasmic reticulum; however, this expression was not found to be related to Na+ currents. SCN1A-silencing reduced MSC proliferation and delayed the cell cycle in the S phase. SCN1A silencing also suppressed the protein levels of CDK2 and AKT (herein referring to total AKT), despite similar mRNA expression, and inhibited AKT phosphorylation in MSCs. A cycloheximide-chase assay showed that SCN1A-silencing induced CDK2 but not AKT protein degradation in MSCs. A proteolysis inhibition assay using epoxomicin, bafilomycin A1 and NH4Cl revealed that both the ubiquitin-proteasome system and the autophagy and endo-lysosome system were irrelevant to CDK2 and AKT protein reduction in SCN1A-silenced MSCs. The AKT inhibitor LY294002 did not affect the degradation and nuclear localization of CDK2 in MSCs. Likewise, the AKT activator SC79 did not attenuate the SCN1A-silencing effects on CDK2 in MSCs. These results suggest that NaV1.1 contributes to the cell cycle of MSCs by regulating the post-translational control of AKT and CDK2., Competing Interests: Competing interests The authors declare no competing or financial interests., (© 2024. Published by The Company of Biologists Ltd.)