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Melanoma is a malignant neoplasm induced by mutations in the DNA of melanocytes and represents more than 80% of deaths from skin cancer in Brazil. This study aimed to investigate the correlation between epidemiological and histopathological aspects of individuals diagnosed with melanoma in a Pathological Anatomy laboratory in the city of Cascavel-PR, during the year 2020. The analysis of histopathological reports from Cascavel or neighboring municipalities in the western region of Paraná and individuals from the public and private health network. A total of 147 cases of melanoma were analyzed, with a predominance of females, aged over 50 years, from the city of Cascavel-PR, with lesions located in the trunk and whose medical care occurred through health plans or agreements. Regarding the histopathological aspects of the lesions, there was a higher incidence of invasive melanoma of the superficial extensive histological type, with Breslow index > 1 mm, Clark level III, mitotic index ≥ 6 mitoses/5 CGA (1 mm2), signs of ulceration and absent regression and Stage IA. Only Clark's Level analysis showed statistical significance (p < 0.05). The data obtained from this study aim to provide subsidies for the realization of new studies on the subject in the region and for the implementation of strategies for the prevention and early diagnosis of cutaneous melanoma. El melanoma es una neoplasia maligna inducida por mutaciones en el ADN de los melanocitos y representa más del 80% de las muertes por cáncer de piel en Brasil. Este estudio tuvo como objetivo investigar la correlación entre los aspectos epidemiológicos e histopatológicos de individuos diagnosticados con melanoma en un laboratorio de Anatomía Patológica en la ciudad de Cascavel-PR, durante el año 2020. El análisis de los informes histopatológicos de Cascavel o municipios vecinos en la región occidental de Paraná y personas de la red de salud pública y privada. Se analizaron un total de 147 casos de melanoma, con predominio del sexo femenino, mayores de 50 años, del municipio de Cascavel-PR, con lesiones localizadas en el tronco y cuya atención médica ocurrió a través de planes o convenios de salud. En cuanto a los aspectos histopatológicos de las lesiones, hubo mayor incidencia de melanoma invasivo del tipo histológico extenso superficial, con índice de Breslow > 1 mm, nivel de Clark III, índice mitótico ≥ 6 mitosis/5 CGA (1 mm2), signos de ulceración y ausencia de regresión y Etapa IA. Solo el análisis de nivel de Clark mostró significación estadística (p < 0,05). Los datos obtenidos de este estudio tienen como objetivo brindar subsidios para la realización de nuevos estudios sobre el tema en la región y para la implementación de estrategias de prevención y diagnóstico precoz del melanoma cutáneo. O melanoma é uma neoplasia maligna induzida por mutações no DNA dos melanócitos e representa mais de 80% das mortes por câncer de pele no Brasil. Esse estudo teve como objetivo investigar a correlação entre os aspectos epidemiológicos e histopatológicos de indivíduos com o diagnóstico de melanoma em um laboratório de Anatomia Patológica na cidade de Cascavel-PR, durante o ano de 2020. Realizou-se a análise dos laudos histopatológicos de provenientes de Cascavel ou de munícipios vizinhos na região oeste do Paraná e de indivíduos oriundos da rede pública e privada de saúde. Foram analisados 147 casos de melanoma, com predomínio do sexo feminino, idade superior aos 50 anos, provenientes do município de Cascavel-PR, com lesões localizadas em tronco e cujo atendimento médico ocorreu por intermédio de planos ou convênios de saúde. No que concerne aos aspectos histopatológicos das lesões, houve maior incidência de melanoma invasivo de tipo histológico extensivo superficial, com índice de Breslow > 1 mm, Nível de Clark III, índice mitótico ≥ 6 mitoses/5 CGA (1 mm2), sinais de ulceração e regressão ausentes e Estadio IA. Apenas analise do Nível de Clark apresentou significância estatística (p < 0,05). Os dados obtidos a partir desse estudo visam fornecer subsídios para a realização de novo estudos sobre o tema na região e para a implementação de estratégias de prevenção e diagnóstico precoce de melanoma cutâneo.

Additional file 5: Fig. S5. Comparison of the growth of transgenic (StAPI5-OE) and non-transgenic potato plants (Wild-type) at the same developmental stages (8-week-old plants).

Additional file 6: Table S1. Values of gas exchange, chlorophyll fluorescence parameters and morphological traits in the resistant cv. Degima, transgenic, and Wilde-type cv. Desiree inoculated with PVA (R/PVA, StAPI5-OE/PVA, and WT/PVA, respectively), PVY (R/PVY, StAPI5-OE/PVY, and WT/PVY), buffer (R/Mock, StAPI5-OE/Mock, and WT/Mock), and without inoculation (R, StAPI5-OE, and WT). The number of replicates in each plant for each treatment was shown as Rep. No. The variables were measured in 21-day-old plants in the growth chamber. Values represent the mean (± SE) of the replicates per treatment. Values within the same row followed by the same letter (s) are not significantly different (Duncan’s multiple range test, P

Additional file 1: Figure S1. The DNA sequencing result of StAPI5 gene in the expression vector pRI201-AN using vector-specific primer M13 forward by Macrogen company using the Sanger technique.

Additional file 2: Fig. S2. PCR analysis of DNA isolated from transgenic plants with CaMV 35 S forward primer (35S-F) and StAPI5 gene reverse specific primer (StAPI5-R) to verify the insertion of the target gene into the potato genome. The expected band length is 754-bp. C: Negative control with non-transgenic potato plant (wild-type); P: positive control PCR reaction with plasmid as template; Lanes no. 1–10: DNA from independent transgenic potato plants and M: 1 kb DNA Ladder.

Additional file 7: Table S2. Sequences of the gene-specific, coat protein of PVY and PVA, and internal control primers.

Additional file 3: Fig. S3. Southern blot analysis of the independent transgenic potato plants overexpressing StAPI5 gene (right) in this image and one other studied gene (left) to confirm the transgene integration into the potato genome and to estimate the transgene copy number. P: the pRI-AN201 binary vector containing the StAPI5 gene (positive control); lanes 1 and 2: independent transgenic potato plants; C: non-transformed potato (negative control)