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Plants belonging to family Paeoniaceae are not only economically important ornamental plants but also medicinal plants used as an important source of traditional Chinese medicine. Owing to the complex network evolution and polyploidy evolution of this family, its systematics and taxonomy are controversial and require a detailed investigation. In this study, three complete chloroplast genomes of sect. Paeonia, one of the sections of Paeonia, were sequenced and then analysed together with 16 other published chloroplast genomes of Paeoniaceae species. The total lengths of the chloroplast genomes of these species were 152,153–154,405 bp. A total of 82–87 protein-coding genes, 31–40 tRNA genes and 8 rRNA genes were annotated. Bioinformatics analysis revealed 61–74 simple sequence repeats (SSRs) in the chloroplast genomes, most of which have A/T base preference. Codon usage analysis showed that A/U-ending codons were more positive than C/G-ending codons, and a slight bias in codon usage was observed in these species. A comparative analysis of these 19 species of Paeoniaceae was then conducted. Fourteen highly variable regions were selected for species relationship study. Phylogenetic analysis revealed that the species of sect. Paeonia gathered in one branch and then divided into different small branches. P. lactiflora, P. anomala, P. anomala subsp. veitchii and P. mairei clustered together. P. intermedia was related to P. obovata and P. obovata subsp. willmottiae. P. emodi was the sister to all other species in the sect. Paeonia. [ABSTRACT FROM AUTHOR]

After solutionizing and aging at 980°C for various durations, the compressive deformation behaviour and mechanisms of the directionally solidified nickel-based superalloy CM247LC are investigated at room temperature. Experimental results show that the yield strength decreases gradually from 991 ± 1.2–672 ± 7.5 MPa with increasing the γ′ precipitate size from 145 ± 2–604 ± 19 nm. Transmission electron microscope observations on the slightly deformed specimens reveal that, in contrast to previous researches, plastic deformation of all the specimens is controlled by strongly coupled dislocations cutting through γ′ precipitates. Based on these experimental results, the relationship between the yield strength and precipitate size is discussed. [ABSTRACT FROM AUTHOR]

• (1)Naive CD8+ T cells were negatively correlated with clinical and pathological parameters of RA. • (2)CD8+ CD28- T cells and CD8+ CD27- T cells were both positively correlated with clinical inflammatory parameters of RA. • (3)TCRγδ + CD8+ T cells and Activated CD8+ T cells may involve in lipid metabolism and lung fibrosis of RA. • (4)These CD8+ T cell subsets may be new biomakers and targets for RA disease evaluation, therapeutic target-selecting, curative effects and prognoses assessment. CD8+ T cells are plentiful in rheumatoid arthritis (RA) and have a important role in it's pathogenesis. Many subsets have been identified in CD8+ T cells, however, the relationship between CD8+ T subpopulations and disease activity of RA is poorly defined. Here we detected different CD8+ T cell subsets in peripheral blood and examined their relationships with clinical features and serological parameters in RA. CD8+ T cell phenotypes and percentages in peripheral blood were determined by flow cytometry in 39 patients with RA. The clinical characteristics and serological parameters of RA patients were collected and DAS28-ESR was measured as indicator of disease activity. Linear regression was performed to assess the correlation of CD8+ T cell subsets with RA clinical variables. Naive CD8+ T cells were significantly and negatively correlated with RA disease activity indicator DAS28-ESR(r2 = 0.1027, p = 0.0468), erythrocyte sedimentation rate (ESR)(r2 = 0.1891, p = 0.0057), clinical disease activity index(CDAI)(r2 = 0.1474, p = 0.0158), simplified disease activity index(SDAI)(r2 = 0.1465, p = 0.0255), and duration(r2 = 0.1247, p = 0.0274). And the percent of naive CD8+ T cells were obviously decreased in RA with high disease activity when compared with RA in low disease activity(p < 0.01). In addition, Our results indicated significant positive correlations between CD8+ CD28- T cells and DAS28-ESR(r2 = 0.1881, p = 0.0058), ESR(r2 = 0.2279, p = 0.0021), c reaction protein (CRP)(r2 = 0.2203, p = 0.0051), CDAI (r2 = 0.1778, p = 0.0075), SDAI (r2 = 0.2618, p = 0.0020), rheumatoid factor(RF)(r2 = 0.1823, p = 0.0067), age(r2 = 0.1968, p = 0.0047), as well as similar positive correlations between CD8+ CD27- T cells and DAS28-ESR(r2 = 0.1661, p = 0.01), ESR(r2 = 0.1586, p = 0.012), CRP(r2 = 0.1778, p = 0.013), CDAI (r2 = 0.1622, p = 0.0110), SDAI(r2 = 0.2316, p = 0.0040), RF(r2 = 0.2097, p = 0.0034), age(r2 = 0.1932, p = 0.0051). Furthermore, interesting results showed observable positive correlations between activated CD8+ T cells and total cholesterol(TC)(r2 = 0.2757, p = 0.0007), triglyceride(TG)(r2 = 0.2886, p = 0.0005), low density lipoprotein(LDL-C)(r2 = 0.09643, p = 0.0264) and Krebs yon denlungen-6(KL-6)(r2 = 0.4171, p = 0.0002). And TCRγδ + CD8+ T cells were also found positively related with total cholesterol(TC)(r2 = 0.5015, p < 0.0001), triglyceride(TG)(r2 = 0.2031, p = 0.0045), and KL-6(r2 = 0.2122, p = 0.0136). Our results suggest that naive CD8+ T cells, CD8+ CD28- T cells, and CD8+ CD27- T cells are obviously correlated with inflammation and disease activity of RA. While activated CD8+ T cells and TCRγδ + CD8+ T cells may involve in lipid metabolism and lung fibrosis of RA. These CD8+ T cell subsets may be new biomarkers and targets for RA disease evaluation, therapeutic target-selecting, curative effects and prognoses assessment. [ABSTRACT FROM AUTHOR]