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Your search keyword '"Ong-Abdullah, Janna"' showing total 14 results
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14 results on '"Ong-Abdullah, Janna"'

8. Bamboo for biomass energy production

Author

Ku Aizuddin, Ku Nur Azwa, primary, Lai, Kok-Song, additional, Baharum, Nadiya Akmal, additional, Thau Lym Yong, Wilson, additional, Ngi Hoon, Lau, additional, Abdul Hamid, Mohd Zahir, additional, Cheng, Wan Hee, additional, and Ong Abdullah, Janna, additional

Published
2022
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13. Vandachostylis‘Sri-Siam’: an intergeneric hybrid with superior qualities as evidenced by its biochemical fragrance and transcriptome profiles

Author

Mohd-Hairul, Ab Rahim, Ong-Abdullah, Janna, Song, Adelene Ai-Lian, Foo, Hooi Ling, Namasivayam, Parameswari, and Rahim, Raha Abdul

Abstract

Vandachostylis‘Sri-Siam’ is a fragrant orchid, suitable as a potted plant for the floriculture and perfumery industries due to its sweet citrus-like fragrance. Fragrance from the flowers was captured using SPME and subjected to GC–MS analysis. A transcriptome library was constructed from the tepals for sequencing using the Illumina MiSeq Desktop Sequencer and for de novo assembly using the CLC Genomic Workbench version 6.0. All de novo assembly sequences were subjected to bioinformatics analyses including BLASTx analysis, functional annotations and mapping of the annotated genes/gene products to the KEGG database using the Blast2go Pro software. GC–MS analysis reveals the fragrance are dominated by 68.73% monoterpenes, 11.26% esters, 4.20% sesquiterpenes and traces of alcohols (1.20%), dissimilar to both its parents. Analysis of the de novo assembly sequences generated 45,585 contigs which identified 14 fragrance-related transcripts. Information on the fragrance constituents and fragrance-related transcripts is deemed important as a reference for current and future work of using this hybrid in the breeding process of commercially valuable fragrant orchids.

Published
2022
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14. Volatile constituents and floral transcriptome analyses provide new insights into fragrance biosynthesis of Vanda 'Tan Chay Yan'.

Author

Mohd-Hairul, Ab. Rahim, Ong Abdullah, Janna, Song, Adelene Ai Lian, Foo, Hooi Ling, Namasivayam, Parameswari, and Rahim, Raha Abdul

Subjects
*TRANSCRIPTOMES, *FLORICULTURE industry, *FOOD aroma, *BIOSYNTHESIS, *GENE mapping, *SESQUITERPENES
Abstract

Vanda 'Tan Chay Yan', a beautiful golden-orange orchid with mild fragrance, is valuable in the floral industry. In this study, the volatile compounds from the flowers were profiled and a transcriptome library was created to search for fragrance-related transcripts. Volatiles from the tepals were captured using a SPME device with PDMS-coated fibre for GC-MS analysis. A transcriptome library was constructed and sequenced using the Illumina MiSeq Desktop Sequencer, de novo assembled using CLC Genomic Workbench version 6.0. BLASTx analysis, functional annotations and gene mapping of the annotated genes to the KEGG database were carried out using the Blast2go Pro software. Twelve compounds were identified in the volatiles including monoterpenes (93.19%), sesquiterpenes (4.32%) and traces of other compounds (2.49%) whereby cis-β-ocimene topped the list as the major constituent (88.41%). De novo assembly of the transcriptome generated a total of 34,027 sequences from which 20 fragrance-related transcripts in the terpenoid and benzenoid/phenylpropanoid biosynthetic pathways were identified. Combined information garnered from the volatiles and fragrance-related transcripts highlights the basic information on the fragrance constituents and biosynthetic pathways of this orchid which are useful for orchid breeders in selection of parents for new hybrids with fragrance as their value-added characteristic for the floriculture industry. [ABSTRACT FROM AUTHOR]

Published
2022
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