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Saccharomyces cerevisiae (SC), a yeast with an extensive history in food and beverage fermentations, is increasingly acknowledged for its multifaceted application in promoting and benefiting all aspects of a ‘One Health’ approach, including the prevention and control of zoonoses. For instance, SC contributes to environmentally sustainable agricultural practices through the reduced use of toxic agents, thus minimizing air and soil pollution while enhancing crop quality. Additionally, this versatile yeast can improve the health of domestic and farm animals, leading to more efficient and sustainable food production, while fostering synergistic impacts across environmental, animal, and human health spheres. Moreover, SC directly applies benefits to human health by promoting improved nutrition, improving gut health through probiotics, as an alternative to antibiotics, and treating gastric disorders. By aligning with several Sustainable Development Goals (SDGs), SC is vital in advancing global health and well-being, environmental sustainability, and responsible consumption and production. This entry illustrates the numerous benefits of SC and highlights its significant impact on a global ‘One Health’ scale, promoting the achievement of SDGs through its unique characteristics and deeper understanding of its contribution to the One Health concept.

The methanogenic strain Mx-05T was isolated from the human fecal microbiome. A phylogenetic analysis based on the 16S rRNA gene and protein marker genes indicated that the strain is affiliated with the order Methanomassiliicoccales. It shares 86.9% 16S rRNA gene sequence identity with Methanomassiliicoccus luminyensis, the only member of this order previously isolated. The cells of Mx-05T were non-motile cocci, with a diameter range of 0.4–0.7 μm. They grew anaerobically and reduced methanol, monomethylamine, dimethylamine, and trimethylamine into methane, using H2 as an electron donor. H2/CO2, formate, ethanol, and acetate were not used as energy sources. The growth of Mx-05T required an unknown medium factor(s) provided by Eggerthella lenta and present in rumen fluid. Mx-05T grew between 30 °C and 40 °C (optimum 37 °C), over a pH range of 6.9–8.3 (optimum pH 7.5), and between 0.02 and 0.34 mol.L−1 NaCl (optimum 0.12 mol.L−1 NaCl). The genome is 1.67 Mbp with a G+C content of 55.5 mol%. Genome sequence annotation confirmed the absence of the methyl branch of the H4MPT Wood–Ljungdahl pathway, as described for other Methanomassiliicoccales members. Based on an average nucleotide identity analysis, we propose strain Mx-05T as being a novel representative of the order Methanomassiliicoccales, within the novel family Methanomethylophilaceae, for which the name Methanomethylophilus alvi gen. nov, sp. nov. is proposed. The type strain is Mx-05T (JCM 31474T).