1. Comparison of Serum, Plasma, and Liver Zinc Measurements by AAS, ICP-OES, and ICP-MS in Diverse Laboratory Settings.
- Author
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Hall, Andrew G, King, Janet C, and McDonald, Christine M
- Subjects
Liver ,Zinc ,Spectrum Analysis ,Laboratories ,Mass Spectrometry ,Accuracy ,Harmonization ,Plasma zinc ,Precision ,Zinc deficiency ,Digestive Diseases ,Biochemistry and Cell Biology ,Medical Biochemistry and Metabolomics ,Toxicology - Abstract
Progress improving zinc nutrition globally is slowed by limited understanding of population zinc status. This challenge is compounded when small differences in measurement can bias the determination of zinc deficiency rates. Our objective was to evaluate zinc analytical accuracy and precision among different instrument types and sample matrices using a standardized method. Participating laboratories analyzed zinc content of plasma, serum, liver samples, and controls, using a standardized method based on current practice. Instrument calibration and drift were evaluated using a zinc standard. Accuracy was evaluated by percent error vs. reference, and precision by coefficient of variation (CV). Seven laboratories in 4 countries running 9 instruments completed the exercise: 4 atomic absorbance spectrometers (AAS), 1 inductively coupled plasma optical emission spectrometer (ICP-OES), and 4 ICP mass spectrometers (ICP-MS). Calibration differed between individual instruments up to 18.9% (p
- Published
- 2022