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A portfolio of six modified 2′‐deoxyribonucleoside triphosphate (dNTP) derivatives derived from 5‐substituted pyrimidine or 7‐substituted 7‐deazapurine bearing different carbohydrate units (d‐glucose, d‐galactose, d‐mannose, l‐fucose, sialic acid and N−Ac‐d‐galactosamine) tethered through propargyl‐glycoside linker was designed and synthesized via the Sonogashira reactions of halogenated dNTPs with the corresponding propargyl‐glycosides. The nucleotides were found to be good substrates for DNA polymerases in enzymatic primer extension and PCR synthesis of modified and hypermodified DNA displaying up to four different sugars. Proof of concept binding study of sugar‐modified oligonucleotides with concanavalin A showed positive effect of avidity and sugar units count. [ABSTRACT FROM AUTHOR]

This addendum to the article "Expedient production of site specifically nucleobase-labelled or hypermodified RNA with engineered thermophilic DNA polymerases" published in Nature Communications provides information about a related work that was published during the peer review process. The related work, titled "Extending the toolbox for RNA biology with SegModTeX: a polymerase-driven method for site-specific and segmental labeling of RNA," was authored by Haslecker, Pham, Glänzer, et al. and published in Nature Communications in 2023. The addendum acknowledges the existence of this related work and provides the necessary citation information. [Extracted from the article]

The RNA-dependent RNA polymerase (RdRp) represents a prominent target in the discovery and development of new antivirotics against RNA viruses, inhibiting the replication process. One of the most targeted RNA viruses of the last years is, without doubt, SARS-CoV-2, the cause of the recent COVID-19 pandemic. HeE1-2Tyr, a known inhibitor of flaviviral RdRp, has been discovered to also have antiviral potency against this coronavirus. In this study, we report three distinct modifications of HeE1-2Tyr: conversion of the core from a benzothiazole to a benzoxazole moiety and two different scaffold simplifications, respectively. We provide a novel synthetic approach and, in addition, evaluate the final molecules in an in vitro polymerase assay for biological activity., (Copyright © 2024, Tóth et al.)