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Shuang Qu,1,* Xiaoli Huang,2,* Xiaoling Guo,3 Zhihai Zheng,1 Tiannan Wei,1 Biyun Chen1 1Department of Hematology, Shengli Clinical Medical College of Fujian Medical University, Fujian Provincial Hospital, Fuzhou, People’s Republic of China; 2Department of Clinical Laboratory Medicine, Shengli Clinical Medical College of Fujian Medical University, Fujian Provincial Hospital, Fuzhou, People’s Republic of China; 3Translational Medicine Centre, the First Affiliated Hospital, Sun Yat-sen University, Guangzhou, People’s Republic of China*These authors contributed equally to this workCorrespondence: Shuang Qu, Email shuangerqu@163.comBackground: Acute myeloid leukemia (AML) is a highly heterogenous disease with varying clinical outcomes among patients. Epithelial-mesenchymal transition (EMT) is an important mechanism underlying cancer metastasis and chemotherapy resistance. However, few EMT-based signatures have been established to predict AML prognosis and treatment efficacy.Methods: By conducting comparative RNA-seq analysis, we discovered the differential expression of EMT genes between AML patients with relapse and those without relapse. Based on the prognostic analysis of the differentially expressed EMT genes, a metastasis-related EMT signature (MEMTs) was constructed. An analysis was conducted on both TARGET and TCGA cohorts to explore the possible association between MEMTs and prognosis in AML. Three separate chemotherapy treatment cohorts were utilized to assess the predictive efficacy of MEMTs for chemotherapy response. In addition, the potential correlation between MEMTs and the tumor microenvironment was also investigated. Finally, random forest analysis and functional experiments were conducted to verify the key MEMTs gene associated with AML metastasis.Results: Based on expression and prognostic analysis, we constructed MEMTs that include three EMT genes (CDH2, LOX, and COL3A1). Our findings suggested that the MEMTs could act as a prognostic factor for AML patients, and furthermore, it proved to be a predictor of their response to chemotherapy. Specifically, high MEMTs was associated with worse prognosis and poor response to chemotherapy, while low MEMTs was linked to better prognosis and higher response rates. Random forest and functional experiments demonstrate that CDH2 is a key gene promoting leukemia cell metastasis among the three MEMTs genes.Conclusion: The identification of MEMTs could potentially act as a predictor for the prognosis and the response to chemotherapy in AML patients. Individual tumor evaluation based on MEMTs could provide personalized treatment options for AML patients in the future.Keywords: acute myeloid leukemia, prognosis, metastasis, epithelial-mesenchymal transition, chemotherapy

This paper mainly focused on the mechanical properties of steel reinforced concrete (SRC) T-shaped columns subjected to axial compression in high temperature. A test program consisting of 8 SRC T-shaped columns was carried out at different influence of specimen parameters. The investigated parameters included axial load ratio, loading eccentricity, loading angle, stirrup spacing and steel ratio. The test results showed that: (1) The failure of axial compression specimens was mainly compression failure. However, the failure of eccentric compression specimens became flexural failure due to the loading eccentricity and loading angle. (2) The fire resistance decreased 50.8% with increasing axial load ratio from 0.2 to 0.6. Decreasing steel ratio from 6.9% to 6.0% led to a decline of 39.4% in the fire resistance. The fire resistance of specimens varied only 1.2% for stirrup spacing from 200 mm to 300 mm. (3) The fire resistance of eccentric compression specimens decreased 8.8%～46.2% compared with the axial compression specimens. The fire resistance of specimens decreased 12.9% with the increase of loading eccentricity from 0 to 40 mm. The fire resistance of specimens decreased 46.2% with the increase of loading angle from 0° to 90°. (4) The influence of axial load ratio and loading angle on the fire resistance was much greater than loading eccentricity. Finally, the fire resistance model of specimens in fire was proposed, and the spatial distribution of fire resistance under the action of two factors was presented, which provided a reference for the post-fire assessment of SRC columns.