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Your search keyword '"Raffa, Vittoria"' showing total 29 results
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29 results on '"Raffa, Vittoria"'

1. Magnetic Nanoparticles for Neural Engineering

Author

Goya, Gerardo F. and Raffa, Vittoria

Subjects
Quantitative Biology - Neurons and Cognition, Physics - Biological Physics
Abstract

Magnetic nanoparticles (MNPs) are the foundation of several new strategies for neural repair and neurological therapies. The fact that a remote force can act on MNPs at the cytoplasmic space constitutes the essence of many new neurotherapeutic concepts. MNPs with a predesigned physicochemical characteristic can interact with external magnetic fields to apply mechanical forces in definite areas of the cell to modulate cellular behaviour. Magnetic actuation to direct the outgrowth on neurons after nerve injury has already demonstrated the therapeutic potential for neural repair. When these magnetic cores are functionalized with molecules such as nerve growth factors or neuroprotective molecules, multifunctional devices can be developed. This chapter will review some of these new nanotechnology-based solutions for neurological diseases, specifically those based on the use of engineered MNPs used for neuroprotection and neuroregeneration. These include the use of MNPs as magnetic actuators to guide neural cells, modulate intracellular transport and stimulate axonal growth after nerve injury., Comment: 35 pages; 6 figures; Chapter 22 of Magnetic Nanoparticles: From Fabrication to Clinical Applications

Published
2024
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7. Integrated opto-fluidic device for high-power living cell exposure and detection

Author

Everhardt, Arnoud S., primary, Konstantinidis, Vasileios, additional, Kapteijn, Paul, additional, Hoekman, Marcel, additional, Konstantinidou, Soultana, additional, D'Amora, Marta, additional, De Carli, Alessandro, additional, Fuso, Francesco, additional, Tantussi, Francesco, additional, Lai, Michele, additional, Raffa, Vittoria, additional, Al-Obaidi, Sadoon M. S., additional, Schilder, Noor A., additional, Heideman, René G., additional, Geuzebroek, Douwe H., additional, and Schreuder, Frederik, additional

Published
2024
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11. Integrated opto-fluidic device for high-power living cell exposure and detection

Author

Gray, Bonnie L., Rapp, Bastian E., Dalton, Colin, Everhardt, Arnoud S., Konstantinidis, Vasileios, Kapteijn, Paul, Hoekman, Marcel, Konstantinidou, Soultana, D'Amora, Marta, De Carli, Alessandro, Fuso, Francesco, Tantussi, Francesco, Lai, Michele, Raffa, Vittoria, Al-Obaidi, Sadoon, Schilder, Noor, Heideman, René, Geuzebroek, Douwe, and Schreuder, Frederik

Published
2024
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12. Microtubules as a signal hub for axon growth in response to mechanical force.

Author

Falconieri, Alessandro, Coppini, Allegra, and Raffa, Vittoria

Subjects
MICROTUBULES, AXONAL transport, AXONS, ORGANELLES, ION channels
Abstract

Microtubules are highly polar structures and are characterized by high anisotropy and stiffness. In neurons, they play a key role in the directional transport of vesicles and organelles. In the neuronal projections called axons, they form parallel bundles, mostly oriented with the plus-end towards the axonal termination. Their physico-chemical properties have recently attracted attention as a potential candidate in sensing, processing and transducing physical signals generated by mechanical forces. Here, we discuss the main evidence supporting the role of microtubules as a signal hub for axon growth in response to a traction force. Applying a tension to the axon appears to stabilize the microtubules, which, in turn, coordinate a modulation of axonal transport, local translation and their cross-talk. We speculate on the possible mechanisms modulating microtubule dynamics under tension, based on evidence collected in neuronal and non-neuronal cell types. However, the fundamental question of the causal relationship between these mechanisms is still elusive because the mechano-sensitive element in this chain has not yet been identified. [ABSTRACT FROM AUTHOR]

Published
2024
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17. Folate Functionalized Boron Nitride Nanotubes and their Selective Uptake by Glioblastoma Multiforme Cells: Implications for their Use as Boron Carriers in Clinical Boron Neutron Capture Therapy

Author

Ciofani, Gianni, Raffa, Vittoria, Menciassi, Arianna, and Cuschieri, Alfred

Abstract

Abstract: Boron neutron capture therapy (BNCT) is increasingly being used in the treatment of several aggressive cancers, including cerebral glioblastoma multiforme. The main requirement for this therapy is selective targeting of tumor cells by sufficient quantities of 10B atoms required for their capture/irradiation with low-energy thermal neutrons. The low content of boron targeting species in glioblastoma multiforme accounts for the difficulty in selective targeting of this very malignant cerebral tumor by this radiation modality. In the present study, we have used for the first time boron nitride nanotubes as carriers of boron atoms to overcome this problem and enhance the selective targeting and ablative efficacy of BNCT for these tumors. Following their dispersion in aqueous solution by noncovalent coating with biocompatible poly-l-lysine solutions, boron nitride nanotubes were functionalized with a fluorescent probe (quantum dots) to enable their tracking and with folic acid as selective tumor targeting ligand. Initial in vitro studies have confirmed substantive and selective uptake of these nanovectors by glioblastoma multiforme cells, an observation which confirms their potential clinical application for BNCT therapy for these malignant cerebral tumors.

Published
2024
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19. Low forces push the maturation of neural precursors into neurons

Author

De Vincentiis, Sara, primary, Baggiani, Matteo, additional, Merighi, Francesca, additional, Cappello, Valentina, additional, Lopane, Jakub, additional, Di Caprio, Mariachiara, additional, Costa, Mario, additional, Mainardi, Marco, additional, Onorati, Marco, additional, and Raffa, Vittoria, additional

Published
2022
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20. Editorial: Perspectives in neuroscience: mechanical forces for the modulation of axonal mechanics and nerve regeneration.

Author

Raffa, Vittoria, Shefi, Orit, and Falconieri, Alessandro

Subjects
NERVOUS system regeneration, AXONS, NEUROSCIENCES, BIOENGINEERING
Abstract

This document is an editorial published in the journal Frontiers in Molecular Neuroscience. It discusses the role of mechanical forces in axonal mechanics and nerve regeneration. The editorial highlights the importance of understanding the biophysical environment and axonal mechanics in promoting axon regeneration post-injury. It presents several studies that explore the effects of mechanical forces on axonal growth and repair, including the role of microtubules and the actin cytoskeleton. The editorial suggests that the future of nerve repair lies in the integration of chemical signaling and biophysical stimuli. [Extracted from the article]

Published
2024
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21. Zebrafish Patient-Derived Xenograft Model to Predict Treatment Outcomes of Colorectal Cancer Patients

Author

Di Franco, Gregorio, primary, Usai, Alice, additional, Piccardi, Margherita, additional, Cateni, Perla, additional, Palmeri, Matteo, additional, Pollina, Luca Emanuele, additional, Gaeta, Raffaele, additional, Marmorino, Federica, additional, Cremolini, Chiara, additional, Dente, Luciana, additional, Massolo, Alessandro, additional, Raffa, Vittoria, additional, and Morelli, Luca, additional

Published
2022
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22. A nanogenetic approach to genome editing

Author

Raffa, Vittoria, primary, Konstantinidou, Soultana, additional, Landi, Elena, additional, Nocilla, Francesco, additional, De Carli, Alessandro, additional, Witt, Dariusz, additional, Lindstaedt, Agnieszka, additional, D'Amora, Marta, additional, Lai, Michele, additional, Schmidt, Tiziana, additional, Pistello, Mauro, additional, Dente, Luciana, additional, Everhardt, Arnoud, additional, Tantussi, Francesco, additional, Fuso, Francesco, additional, Gabellini, Chiara, additional, Barski, Piotr, additional, and Freer, Giulia, additional

Published
2022
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23. Axonal plasticity in response to active forces generated through magnetic nanopulling

Author

Falconieri, Alessandro, primary, De Vincentiis, Sara, additional, Cappello, Valentina, additional, Convertino, Domenica, additional, Ghignoli, Samuele, additional, Figoli, Sofia, additional, Luin, Stefano, additional, Català-Castro, Frederic, additional, Marchetti, Laura, additional, Borello, Ugo, additional, Krieg, Michael, additional, and Raffa, Vittoria, additional

Published
2022
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24. Effects of Metal Oxide Nanoparticles in Zebrafish

Author

d’Amora, Marta, Schmidt, Tiziana Julia Nadjeschda, Konstantinidou, Soultana, Raffa, Vittoria, De Angelis, Francesco, and Tantussi, Francesco

Subjects
Article Subject
Abstract

Metal oxide nanoparticles (MO NPs) are increasingly employed in many fields with a wide range of applications from industries to drug delivery. Due to their semiconducting properties, metal oxide nanoparticles are commonly used in the manufacturing of several commercial products available in the market, including cosmetics, food additives, textile, paint, and antibacterial ointments. The use of metallic oxide nanoparticles for medical and cosmetic purposes leads to unavoidable human exposure, requiring a proper knowledge of their potentially harmful effects. This review offers a comprehensive overview of the possible toxicity of metallic oxide nanoparticles in zebrafish during both adulthood and growth stages, with an emphasis on the role of oxidative stress.

Published
2022
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26. Zebrafish Patient-Derived Xenografts Identify Chemo-Response in Pancreatic Ductal Adenocarcinoma Patients

Author

Usai, Alice, primary, Di Franco, Gregorio, additional, Piccardi, Margherita, additional, Cateni, Perla, additional, Pollina, Luca Emanuele, additional, Vivaldi, Caterina, additional, Vasile, Enrico, additional, Funel, Niccola, additional, Palmeri, Matteo, additional, Dente, Luciana, additional, Falcone, Alfredo, additional, Giunchi, Dimitri, additional, Massolo, Alessandro, additional, Raffa, Vittoria, additional, and Morelli, Luca, additional

Published
2021
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28. Axonal plasticity in response to active forces generated through magnetic nano-pulling

Author

Falconieri, Alessandro, De Vincentiis, Sara, Cappello, Valentina, Convertino, Domenica, Das, Ravi, Ghignoli, Samuele, Figoli, Sofia, Luin, Stefano, Català-Castro, Frederic, Marchetti, Laura, Borello, Ugo, Krieg, Michael, and Raffa, Vittoria

Abstract

Mechanical force is crucial in guiding axon outgrowth before and after synapse formation. This process is referred to as “stretch growth.” However, how neurons transduce mechanical input into signaling pathways remains poorly understood. Another open question is how stretch growth is coupled in time with the intercalated addition of new mass along the entire axon. Here, we demonstrate that active mechanical force generated by magnetic nano-pulling induces remodeling of the axonal cytoskeleton. Specifically, the increase in the axonal density of microtubules induced by nano-pulling leads to an accumulation of organelles and signaling vesicles, which, in turn, promotes local translation by increasing the probability of assembly of the “translation factories.” Modulation of axonal transport and local translation sustains enhanced axon outgrowth and synapse maturation.

Published
2022
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29. Axonal plasticity in response to active forces generated through magnetic nano-pulling

Author

Alessandro Falconieri, Sara De Vincentiis, Valentina Cappello, Domenica Convertino, Ravi Das, Samuele Ghignoli, Sofia Figoli, Stefano Luin, Frederic Català-Castro, Laura Marchetti, Ugo Borello, Michael Krieg, Vittoria Raffa, Falconieri, Alessandro, De Vincentiis, Sara, Cappello, Valentina, Convertino, Domenica, Das, Ravi, Ghignoli, Samuele, Figoli, Sofia, Luin, Stefano, Català-Castro, Frederic, Marchetti, Laura, Borello, Ugo, Krieg, Michael, and Raffa, Vittoria

Subjects
axon, magnetic nanoparticles, Neuroscience [CP], CP: Cell biology, CP: Neuroscience, force, local translation, microtubules, outgrowth, remodeling, synapse, magnetic nanoparticle, Cell biology [CP], Settore BIO/11 - Biologia Molecolare, Settore FIS/07 - Fisica Applicata(Beni Culturali, Ambientali, Biol.e Medicin), General Biochemistry, Genetics and Molecular Biology, Settore FIS/03 - Fisica della Materia, microtubule
Abstract

Mechanical force is crucial in guiding axon outgrowth before and after synapse formation. This process is referred to as "stretch growth."However, how neurons transduce mechanical input into signaling pathways remains poorly understood. Another open question is how stretch growth is coupled in time with the interca-lated addition of new mass along the entire axon. Here, we demonstrate that active mechanical force gener-ated by magnetic nano-pulling induces remodeling of the axonal cytoskeleton. Specifically, the increase in the axonal density of microtubules induced by nano-pulling leads to an accumulation of organelles and signaling vesicles, which, in turn, promotes local translation by increasing the probability of assembly of the "translation factories."Modulation of axonal transport and local translation sustains enhanced axon outgrowth and synapse maturation.

Published
2023

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