Search

Your search keyword '"Rikhav P. Gala"' showing total 3 results
Start Over Author "Rikhav P. Gala" Publication Year Range Last 3 years
3 results on '"Rikhav P. Gala"'

1. Assessment of In Vitro Immunostimulatory Activity of an Adjuvanted Whole-Cell Inactivated Neisseria gonorrhoeae Microparticle Vaccine Formulation

Author

Priyal Bagwe, Lotika Bajaj, Rikhav P. Gala, Martin J. D‘Souza, and Susu M. Zughaier

Subjects
Neisseria gonorrhoeae, microparticles, vaccines, antigen presentation, autophagy, gonorrhea, Medicine
Abstract

The emergence of drug-resistant gonorrhea infections worldwide combined with the lack of a vaccine is alarming. We prepared a novel microparticulate (MP) vaccine formulation using whole-cell inactivated Neisseria gonorrhoeae as the vaccine antigen, with Alum and AddaVax™ as vaccine adjuvants. The adjuvanted vaccine MP formulation was assessed for in vitro immunostimulatory activity, autophagy, and antigen presentation ability. The data shows that the adjuvanted gonococci vaccine MP enhanced autophagy induction in antigen presenting cells (APCs) compared to gonococci vaccine MP without adjuvants, which is important for enhancing antigen presentation. In addition, the adjuvanted vaccine formulation increased the surface expression of antigen presenting molecules MHCI and MHCII as well as co-stimulatory molecules CD40 and CD86 on the surface of dendritic cells. In addition, the gonococci vaccine microparticles at lower doses did not significantly increase the expression of the death receptor CD95 in APCs, which when elevated leads to suboptimal antigen presentation and reduced immune responses. The adjuvanted whole-cell inactivated gonococci microparticle vaccine formulation enhanced antigen uptake, processing, and antigen presentation.

Published
2022
Full Text
View/download PDF

2. Needleless administration of advanced therapies into the skin via the appendages using a hypobaric patch

Author

Faiza Benaouda, Ricardo Inacio, Chui Hua Lim, Haeeun Park, Thomas Pitcher, Mohamed A. Alhnan, Mazen M. S. Aly, Khuloud T Al-Jamal, Ka-lung Chan, Rikhav P. Gala, Daniel Sebastia-Saez, Liang Cui, Tao Chen, Julie Keeble, and Stuart A. Jones

Subjects
Mice, Vaccines, Multidisciplinary, Needles, Swine, Skin Absorption, Animals, Administration, Cutaneous, Rats, Skin
Abstract

Advanced therapies are commonly administered via injection even when they act within the skin tissue, and this increases the chances of off-target effects. Here we report the use of a skin patch containing a hypobaric chamber that induces skin dome formation to enable needleless delivery of advanced therapies directly into porcine, rat, and mouse skin. Finite element method modeling showed that the hypobaric chamber in the patch opened the skin appendages by 32%, thinned the skin, and compressed the appendage wall epithelia. These changes allowed direct delivery of an H1N1 vaccine antigen and a diclofenac nanotherapeutic into the skin. Fluorescence imaging and infrared mapping of the skin showed needleless delivery via the appendages. The in vivo utility of the patch was demonstrated by a superior immunoglobulin G response to the vaccine antigen in mice compared to intramuscular injection and a 70% reduction in rat paw swelling in vivo over 5 h with diclofenac without skin histology changes.

Published
2022

3. Preclinical evaluation of a microparticle-based transdermal vaccine patch against metastatic breast cancer

Author

Rokon Uz, Zaman, Rikhav P, Gala, Amit, Bansal, Priyal, Bagwe, and Martin J, D'Souza

Subjects
Mice, Antigens, Neoplasm, Cell Line, Tumor, Neoplasms, Animals, Pharmaceutical Science, Female, CD8-Positive T-Lymphocytes, Cancer Vaccines, Cyclophosphamide
Abstract

Breast cancer is the number one cause of cancer-related deaths among females. Current chemotherapy targets both tumor and normal cells, leading to pronounced side effects. Therefore, therapeutic vaccines acting against specific cancer cells would be the choice of treatment. We prepared microparticles entrapping the antigens obtained from a murine metastatic breast cancer cell line, 4 T1 using the spray drying technology. These microparticles were incorporated into microneedle patches to deliver to the animals for the efficacy study. An antineoplastic drug, cyclophosphamide, in a very low dose has been found to inhibit the immunosuppressive regulatory T cells (T

Published
2022

Catalog

Books, media, physical & digital resources
See catalog results