71 results on '"Rout, M."'
Search Results
2. Foot-and-mouth disease in mithun, yak, cattle-yak hybrids and cattle in the north-eastern states of India during 2021-2022
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Rout, M., Garam, G.B., Lama, Rinchin, Deka, P., Tripathy, J.P., Giri, P., Acharya, R., Subramaniam, S., Mohapatra, J.K., and Singh, R.P.
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- 2024
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3. Serological evidence of foot-and-mouth disease virus nonstructural protein antibodies in Indian elephants (Elephas maximus indicus)
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Rout, M., Deka, P., Nair, N.S., Karikalan, M., Manjunatha, V.5, Sahoo, N., Sharma, A.K., Mohapatra, J.K., and Singh, R.P.
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- 2023
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4. Report of Camallanus trispinosus infestation in a captive indian star tortoise (Geochelone elegans)
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Manjunatha, V., Rout, M., Shankar, B.P., Sreevatsava, V., Shankar, K.S. Uma, Sripad, K., and Venkatesha, M.D.
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- 2023
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5. Pneumonia Caused by Klebsiella pneumoniae in a Pig-tailed Macaque (Macaca leonina)
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Manjunatha, V., Rout, M., Shankar, B.P., Vijay, N., Umashankar, K.S., and Hegde, R.
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- 2023
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6. Detection of multiple hepatic peribiliary cysts in captive lions (Panthera leo)
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Manjunatha, V., Rout, M., Jaisingh, N., Shankar, B.P., and Umashankar, K.S.
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- 2023
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7. A copro-parasitological surveillance on diverse captive wild avian species
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Manjunatha, V., Rout, M., Sreevatsava, V., Kshamaa, L.M., Umashankar, Shankar, B.P., and Byregowda, S.M.
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- 2023
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8. Prediction of compressive strength of glass fiber-reinforced self-compacting concrete interpretable by machine learning algorithms
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Gogineni, Abhilash, Rout, M. K. Diptikanta, and Shubham, Kumar
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- 2024
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9. Evaluating machine learning algorithms for predicting compressive strength of concrete with mineral admixture using long short-term memory (LSTM) Technique
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Gogineni, Abhilash, Rout, M. K. Diptikanta, and Shubham, Kumar
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- 2024
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10. An integrated evaluation of waste materials containing recycled asphalt fine aggregates using central composite design
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Rout, M. K. Diptikanta, Shubham, Kumar, Biswas, Sabyasachi, and Sinha, Abdhesh Kumar
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- 2024
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11. Evaluation of Mechanical Properties of Rigid Pavement with High RAP Content
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Diptikanta Rout, M. K., Biswas, Sabyasachi, Sinha, Abdhesh Kumar, di Prisco, Marco, Series Editor, Chen, Sheng-Hong, Series Editor, Vayas, Ioannis, Series Editor, Kumar Shukla, Sanjay, Series Editor, Sharma, Anuj, Series Editor, Kumar, Nagesh, Series Editor, Wang, Chien Ming, Series Editor, Anjaneyulu, M. V. L. R., editor, Harikrishna, M., editor, Arkatkar, Shriniwas S., editor, and Veeraragavan, A., editor
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- 2023
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12. Medical Image Classifications: Deep Learning Prospective
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Biswal, P., Behera, S., Jaiswal, R., Sarma, M., Rout, M., Barik, Rabindra Kumar, Kacprzyk, Janusz, Series Editor, Gomide, Fernando, Advisory Editor, Kaynak, Okyay, Advisory Editor, Liu, Derong, Advisory Editor, Pedrycz, Witold, Advisory Editor, Polycarpou, Marios M., Advisory Editor, Rudas, Imre J., Advisory Editor, Wang, Jun, Advisory Editor, Khanna, Ashish, editor, Gupta, Deepak, editor, Kansal, Vineet, editor, Fortino, Giancarlo, editor, and Hassanien, Aboul Ella, editor
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- 2023
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13. Evaluation of Mechanical Properties of Rigid Pavement with High RAP Content
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Diptikanta Rout, M. K., primary, Biswas, Sabyasachi, additional, and Sinha, Abdhesh Kumar, additional
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- 2022
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14. Medical Image Classifications: Deep Learning Prospective
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Biswal, P., primary, Behera, S., additional, Jaiswal, R., additional, Sarma, M., additional, Rout, M., additional, and Barik, Rabindra Kumar, additional
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- 2022
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15. Fatal Canine Adenovirus Type 1 Infection in Dhole (Cuon alpinus) Pups
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Manjunatha, V., primary, Rout, M., additional, Srinivas, U.K., additional, Kshamaa, L.M., additional, Sujay, S., additional, Sripad, K., additional, and Byregowda, S.M., additional
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- 2024
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16. Prediction of compressive strength of glass fiber-reinforced self-compacting concrete interpretable by machine learning algorithms
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Gogineni, Abhilash, primary, Rout, M. K. Diptikanta, additional, and Shubham, Kumar, additional
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- 2023
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17. Evaluating machine learning algorithms for predicting compressive strength of concrete with mineral admixture using long short-term memory (LSTM) Technique
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Gogineni, Abhilash, primary, Rout, M. K. Diptikanta, additional, and Shubham, Kumar, additional
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- 2023
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18. Feasibility Study of Reclaimed Asphalt Pavements (RAP) as Recycled Aggregates Used in Rigid Pavement Construction
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Rout, M. K. Diptikanta, primary, Sahdeo, Surya Kant, additional, Biswas, Sabyasachi, additional, Roy, Krishanu, additional, and Sinha, Abdhesh Kumar, additional
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- 2023
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19. Detection of Multiple Hepatic Peribiliary Cysts in Captive Lions (Panthera leo)
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Manjunatha, V., primary, Rout, M., additional, Jaisingh, N., additional, Shankar, B.P., additional, and Umashankar, K.S., additional
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- 2022
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20. Planet Hunters TESS IV: a massive, compact hierarchical triple star system TIC 470710327
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Eisner, N L, primary, Johnston, C, additional, Toonen, S, additional, Frost, A J, additional, Janssens, S, additional, Lintott, C J, additional, Aigrain, S, additional, Sana, H, additional, Abdul-Masih, M, additional, Arellano-Córdova, K Z, additional, Beck, P G, additional, Bordier, E, additional, Cannon, E, additional, Escorza, A, additional, Fabry, M, additional, Hermansson, L, additional, Howell, S B, additional, Miller, G, additional, Sheyte, S, additional, Alhassan, S, additional, Baeten, E M L, additional, Barnet, F, additional, Bean, S J, additional, Bernau, M, additional, Bundy, D M, additional, Di Fraia, M Z, additional, Emralino, F M, additional, Goodwin, B L, additional, Hermes, P, additional, Hoffman, T, additional, Huten, M, additional, Janíček, R, additional, Lee, S, additional, Mazzucato, M T, additional, Rogers, D J, additional, Rout, M P, additional, Sejpka, J, additional, Tanner, C, additional, Terentev, I A, additional, and Urvoy, D, additional
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- 2022
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21. A species-independent indirect-ELISA for detection of antibodies to the non-structural protein 2B of foot-and-mouth disease virus.
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Biswal JK, Das S, Mohapatra JK, Rout M, Ranjan R, and Singh RP
- Abstract
Diagnostic assays that are able to detect foot-and-mouth disease (FMD) virus infection in the vaccinated population are essential tools in the progressive control pathway for the FMD. However, testing of serum samples using a single diagnostic assay may not completely substantiate freedom from the virus infection. Therefore, viral non-structural proteins (NSPs)-based various serological assays have been developed for the detection of FMD infection. Nevertheless, the NSPs-based ELISAs have been developed in the indirect-ELISA format, thereby necessitating the use of species-specific conjugated secondary-antibodies for the detection of anti-NSP antibodies in various FMD-susceptible species. Therefore, this study presents a novel recombinant 2B-NSP-based indirect ELISA, employing HRP-conjugated protein-A/G detection system which can detect anti-NSPs antibodies from multiple FMD-susceptible species in a single ELISA platform. Recombinant 2B (r2B) protein was expressed as His-SUMO tagged protein in the E. Coli cells and purified using NI-NTA affinity column chromatography. Using the r2B protein and HRP-conjugated protein A/G, an indirect ELISA was developed and validated for the detection of anti-2B antibodies in serum samples collected from multiple FMD-susceptible animal species with known FMD status. Further, a resampling based statistical technique has been reported for determination of optimal cut-off value for the diagnostic assay. Through this technique, the optimal cut-off of 44 percentage of positivity value was determined for the assay. At this optimal cut-off value, the developed diagnostic assay provided diagnostic sensitivity, specificity, and accuracy, positive and negative predictive values (PPV and NPV) of 92.35 %, 98.41 %, 95.21 %, 98.58 %, and 91.67 %, respectively. The assay was validated further by analyzing random serum samples collected across multi-locations in India. The assay can be used as a single platform for testing serum samples from different species of FMDV-susceptible animals and will be useful for NSP-based serosurveillance of FMDV., Competing Interests: Declaration of competing interest The authors declare no competing financial and non-financial interest., (Copyright © 2024 International Alliance for Biological Standardization. Published by Elsevier Ltd. All rights reserved.)
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- 2024
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22. Automatic Chemical Profiling of Wine by Proton Nuclear Magnetic Resonance Spectroscopy.
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Lee BL, Rout M, Dong Y, Lipfert M, Berjanskii M, Shahin F, Bhattacharyya D, Selim A, Mandal R, and Wishart DS
- Abstract
We report the development of MagMet-W (magnetic resonance for metabolomics of wine), a software program that can automatically determine the chemical composition of wine via
1 H nuclear magnetic resonance (NMR) spectroscopy. MagMet-W is an extension of MagMet developed for the automated metabolomic analysis of human serum by1 H NMR. We identified 70 compounds suitable for inclusion into MagMet-W. We then obtained 1D1 H NMR reference spectra of the pure compounds at 700 MHz and incorporated these spectra into the MagMet-W compound library. The processing of the wine NMR spectra and profiling of the 70 wine compounds were then optimized based on manual1 H NMR analysis. MagMet-W can automatically identify 70 wine compounds in most wine samples and can quantify them to 10-15% of the manually determined concentrations, and it can analyze multiple spectra simultaneously, at 10 min per spectrum. The MagMet-W Web server is available at https://www.magmet.ca., Competing Interests: The authors declare no competing financial interest., (© 2024 The Authors. Published by American Chemical Society.)- Published
- 2024
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23. Recombinant 3AB3 nonstructural protein-based indirect ELISA for detection of foot-and-mouth disease virus infection-elicited antibodies in goat.
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Rout M, Pandey LK, Prusty BR, Samanta R, Mohapatra JK, and Singh RP
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Foot-and-mouth disease (FMD) is one of the most important animal diseases of economic significance globally. It is a highly infectious and contagious disease of cloven-hoofed animals including sheep and goat. For sero-diagnosis of FMD, recombinant antigen-based assays are considered as alternatives to conventional approaches such as the liquid phase blocking ELISA (LPBE). The early interventions towards control measures cannot be implemented unless the disease gets promptly diagnosed. It is relatively difficult to clinically diagnose FMD in goat due to the usual milder form or unapparent nature of symptoms. Under such situations where clinical samples are not available, demonstration of infection-specific FMD virus (FMDV) antibodies in serum sample may help identifying the animals exposed to the virus in retrospect. Antibody to 3AB nonstructural protein (NSP) has been considered to be the most reliable indicator for FMD diagnosis. The current study extended the earlier designed recombinant 3AB3 protein-based indirect ELISA originally validated on bovine serum samples to testing serum samples of goat. The performance of the indirect ELISA was validated using internationally accepted PrioCHECK
® FMDV NS kit. The overall diagnostic sensitivity (DSn) of the indirect ELISA was estimated to be 95.52% (619/648), while the diagnostic specificity (DSp) on naïve and vaccinated animals varied at 98.06% (557/568) and 94.15% (435/462), respectively. In India, where FMD is prevalent and the goat population is so high, this 'in-house' optimized assay can be considered to be an adjunct in sero-epidemiological investigation of FMD in goat., (© 2024. The Author(s), under exclusive licence to Springer Nature B.V.)- Published
- 2024
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24. A 'te ao Māori' disaster risk reduction framework.
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Rout M, Awatere S, Reid J, Campbell E, Huang A, and Warmenhoven T
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- Humans, Decision Making, Disasters, Maori People, New Zealand, Disaster Planning organization & administration, Risk Reduction Behavior
- Abstract
An ongoing change in legislation means decision-makers in Aotearoa New Zealand need to incorporate 'mātauranga' (Māori knowledge/knowledge system) in central and local government legislation and strategy. This paper develops a 'te ao Māori' (Māori worldview) disaster risk reduction (DRR) framework for non-Māori decision-makers to guide them through this process. This 'interface framework' will function as a Rosetta Stone between the 'two worlds'. It intends to help central and local officials trained in Western knowledge-based disciplines by translating standard DRR concepts into a te ao Māori DRR framework. It draws on previous work examining Māori DRR thinking to create a novel framework that can help these stakeholders when they are converting higher-level theoretical insights from mātauranga Māori into more practical 'on the ground' applications. This type of interface is essential: while Indigenous knowledge's utility is increasingly recognised nationally and internationally, a gap remains between this acknowledgement and its practical and applied integration into emergency management legislation and strategy., (© 2024 The Authors. Disasters published by John Wiley & Sons Ltd on behalf of ODI.)
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- 2024
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25. Classical molecular dynamics simulation identifies catechingallate as a promising antiviral polyphenol against MPOX palmitoylated surface protein.
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Mishra S, Rout M, Singh MK, Dehury B, and Pati S
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- Catechin chemistry, Catechin analogs & derivatives, Catechin pharmacology, Molecular Docking Simulation, Antiviral Agents chemistry, Antiviral Agents pharmacology, Molecular Dynamics Simulation, Polyphenols chemistry, Polyphenols pharmacology
- Abstract
Cumulative global prevalence of the emergent monkeypox (MPX) infection in the non-endemic countries has been professed as a global public health predicament. Lack of effective MPX-specific treatments sets the baseline for designing the current study. This research work uncovers the effective use of known antiviral polyphenols against MPX viral infection, and recognises their mode of interaction with the target F13 protein, that plays crucial role in formation of enveloped virions. Herein, we have employed state-of-the-art machine learning based AlphaFold2 to predict the three-dimensional structure of F13 followed by molecular docking and all-atoms molecular dynamics (MD) simulations to investigate the differential mode of F13-polyphenol interactions. Our extensive computational approach identifies six potent polyphenols Rutin, Epicatechingallate, Catechingallate, Quercitrin, Isoquecitrin and Hyperoside exhibiting higher binding affinity towards F13, buried inside a positively charged binding groove. Intermolecular contact analysis of the docked and MD simulated complexes divulges three important residues Asp
134 , Ser137 and Ser321 that are observed to be involved in ligand binding through hydrogen bonds. Our findings suggest that ligand binding induces minor conformational changes in F13 to affect the conformation of the binding site. Concomitantly, essential dynamics of the six-MD simulated complexes reveals Catechin gallate, a known antiviral agent as a promising polyphenol targeting F13 protein, dominated with a dense network of hydrophobic contacts. However, assessment of biological activities of these polyphenols need to be confirmed through in vitro and in vivo assays, which may pave the way for development of new novel antiviral drugs., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)- Published
- 2024
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26. Foot-and-mouth disease-associated myocarditis is age dependent in suckling calves.
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Deka P, Das S, Hazarika R, Kayaga R, Dutta B, Deka A, Barman U, Ahmed R, Islam N, Sarma M, Deka I, Rout M, Sharma K, and Sharma RK
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- Animals, Cattle, Cattle Diseases virology, Cattle Diseases blood, Cattle Diseases pathology, Foot-and-Mouth Disease Virus pathogenicity, Foot-and-Mouth Disease Virus isolation & purification, Animals, Suckling, Age Factors, Aspartate Aminotransferases blood, Male, L-Lactate Dehydrogenase blood, Myocarditis veterinary, Myocarditis virology, Myocarditis pathology, Foot-and-Mouth Disease virology, Foot-and-Mouth Disease pathology
- Abstract
Myocarditis is considered a fatal form of foot-and-mouth disease (FMD) in suckling calves. In the present study, a total of 17 calves under 4 months of age and suspected clinically for FMD were examined for clinical lesions, respiratory rate, heart rate, and heart rhythm. Lesion samples, saliva, nasal swabs, and whole blood were collected from suspected calves and subjected to Sandwich ELISA and reverse transcription multiplex polymerase chain reaction (RT-mPCR) for detection and serotyping of FMD virus (FMDV). The samples were found to be positive for FMDV serotype "O". Myocarditis was suspected in 6 calves based on tachypnoea, tachycardia, and gallop rhythm. Serum aspartate aminotransferase (AST), creatinine kinase myocardial band (CK-MB) and lactate dehydrogenase (LDH), and cardiac troponins (cTnI) were measured. Mean serum AST, cTn-I and LDH were significantly higher (P < 0.001) in < 2 months old FMD-infected calves showing clinical signs suggestive of myocarditis (264.833 ± 4.16; 11.650 ± 0.34 and 1213.33 ± 29.06) than those without myocarditis (< 2 months old: 110.00 ± 0.00, 0.06 ± 0.00, 1050.00 ± 0.00; > 2 months < 4 months: 83.00 ± 3.00, 0.05 ± 0.02, 1159.00 ± 27.63) and healthy control groups (< 2 months old: 67.50 ± 3.10, 0.047 ± 0.01, 1120.00 ± 31.62; > 2 months < 4 months: 72.83 ± 2.09, 0.47 ± 0.00, 1160.00 ± 18.44). However, mean serum CK-MB did not differ significantly amongst the groups. Four calves under 2 months old died and a necropsy revealed the presence of a pathognomic gross lesion of the myocardial form of FMD known as "tigroid heart". Histopathology confirmed myocarditis. This study also reports the relevance of clinical and histopathological findings and biochemical markers in diagnosing FMD-related myocarditis in suckling calves., (© 2024. The Author(s).)
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- 2024
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27. Machine learning and classical MD simulation to identify inhibitors against the P37 envelope protein of monkeypox virus.
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Rout M, Dey S, Mishra S, Panda S, Singh MK, Sinha R, Dehury B, and Pati S
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- Protein Binding, Binding Sites, Hydrogen Bonding, Hydrophobic and Hydrophilic Interactions, Molecular Dynamics Simulation, Antiviral Agents chemistry, Antiviral Agents pharmacology, Molecular Docking Simulation, Viral Envelope Proteins chemistry, Viral Envelope Proteins metabolism, Viral Envelope Proteins antagonists & inhibitors, Machine Learning, Monkeypox virus chemistry, Monkeypox virus drug effects
- Abstract
Monkeypox virus (MPXV) outbreak is a serious public health concern that requires international attention. P37 of MPXV plays a pivotal role in DNA replication and acts as one of the promising targets for antiviral drug design. In this study, we intent to screen potential analogs of existing FDA approved drugs of MPXV against P37 using state-of-the-art machine learning and computational biophysical techniques. AlphaFold2 guided all-atoms molecular dynamics simulations optimized P37 structure is used for molecular docking and binding free energy calculations. Similar to members of Phospholipase-D family , the predicted P37 structure also adopts a β-α-β-α-β sandwich fold, harbouring strongly conserved HxKxxxxD motif. The binding pocket comprises of Tyr48, Lys86, His115, Lys117, Ser130, Asn132, Trp280, Asn240, His325, Lys327 and Tyr346 forming strong hydrogen bonds and dense hydrophobic contacts with the screened analogs and is surrounded by positively charged patches. Loops connecting the two domains and C-terminal region exhibit high degree of flexibility. In some structural ensembles, the partial disorderness in the C-terminal region is presumed to be due to its low confidence score, acquired during structure prediction. Transition from loop to β-strands (244-254 aa) in P37-Cidofovir and its analog complexes advocates the need for further investigations. MD simulations support the accuracy of the molecular docking results, indicating the potential of analogs as potent binders of P37. Taken together, our results provide preferable understanding of molecular recognition and dynamics of ligand-bound states of P37, offering opportunities for development of new antivirals against MPXV. However, the need of in vitro and in vivo assays for confirmation of these results still persists.Communicated by Ramaswamy H. Sarma.
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- 2024
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28. Polygenic Risk Score Assessment for Coronary Artery Disease in Asian Indians.
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Rout M, Tung GK, Singh JR, Mehra NK, Wander GS, Ralhan S, and Sanghera DK
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We evaluated the performance of various polygenic risk score (PRS) models derived from European (EU), South Asian (SA), and Punjabi Asian Indians (AI) studies on 13,974 subjects from AI ancestry. While all models successfully predicted Coronary artery disease (CAD) risk, the AI, SA, and EU + AI were superior predictors and more transportable than the EU model; the predictive performance in training and test sets was 18% and 22% higher in AI and EU + AI models, respectively than in EU. Comparing individuals with extreme PRS quartiles, the AI and EU + AI captured individuals with high CAD risk showed 2.6 to 4.6 times higher efficiency than the EU. Interestingly, including the clinical risk score did not significantly change the performance of any genetic model. The enrichment of diversity variants in EU PRS improves risk prediction and transportability. Establishing population-specific normative and risk factors and inclusion into genetic models would refine the risk stratification and improve the clinical utility of CAD PRS., (© 2024. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)
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- 2024
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29. All-atoms molecular dynamics study to screen potent efflux pump inhibitors against KpnE protein of Klebsiella pneumoniae .
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Dey S, Rout M, Pati S, Singh MK, Dehury B, and Subudhi E
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- Klebsiella pneumoniae, Molecular Docking Simulation, Escherichia coli metabolism, Lipid Bilayers chemistry, Antiporters metabolism, Molecular Dynamics Simulation, Escherichia coli Proteins metabolism
- Abstract
The Small Multidrug Resistance efflux pump protein KpnE, plays a pivotal role in multi-drug resistance in Klebsiella pneumoniae . Despite well-documented study of its close homolog, EmrE, from Escherichia coli , the mechanism of drug binding to KpnE remains obscure due to the absence of a high-resolution experimental structure. Herein, we exclusively elucidate its structure-function mechanism and report some of the potent inhibitors through drug repurposing. We used molecular dynamics simulation to develop a dimeric structure of KpnE and explore its dynamics in lipid-mimetic bilayers. Our study identified both semi-open and open conformations of KpnE, highlighting its importance in transport process. Electrostatic surface potential map suggests a considerable degree of similarity between KpnE and EmrE at the binding cleft, mostly occupied by negatively charged residues. We identify key amino acids Glu14, Trp63 and Tyr44, indispensable for ligand recognition. Molecular docking and binding free energy calculations recognizes potential inhibitors like acarbose, rutin and labetalol. Further validations are needed to confirm the therapeutic role of these compounds. Altogether, our membrane dynamics study uncovers the crucial charged patches, lipid-binding sites and flexible loop that could potentiate substrate recognition, transport mechanism and pave the way for development of novel inhibitors against K. pneumoniae .Communicated by Ramaswamy H. Sarma.
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- 2024
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30. Lipid and cholesterols modulate the dynamics of SARS-CoV-2 viral ion channel ORF3a and its pathogenic variants.
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Rout M, Mishra S, Panda S, Dehury B, and Pati S
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- Humans, Ion Channels, Cholesterol, SARS-CoV-2 genetics, COVID-19 genetics
- Abstract
SARS-CoV-2 accessory protein, ORF3a is a putative ion channel which immensely contributes to viral pathogenicity by modulating host immune responses and virus-host interactions. Relatively high expression of ORF3a in diseased individuals and implication with inflammasome activation, apoptosis and autophagy inhibition, ratifies as an effective target for developing vaccines and therapeutics. Herein, we present the elusive dynamics of ORF3a-dimeric state using all-atoms molecular dynamics (MD) simulations at μ-seconds scale in a heterogeneous lipid-mimetic system in multiple replicates. Additionally, we also explore the effect of non-synonymous pathogenic mutations on ORF3a ion channel activity and viral pathogenicity in different SARS-CoV-2 variants using various structure-based protein stability (ΔΔG) tools and computational saturation mutagenesis. Our study ascertains the role of phosphatidylcholines and cholesterol in modulating the structure of ORF3a, which perturbs the size and flexibility of the polar cavity that allows permeation of large cations. Discrete trend in ion channel pore radius and area per lipid arises the premise that presence of lipids might also affect the overall conformation of ORF3a. MD structural-ensembles, in some replicates rationalize the crucial role of TM2 in maintaining the native structure of ORF3a. We also infer that loss of structural stability primarily grounds for pathogenicity in more than half of the pathogenic variants of ORF3a. Overall, the effect of mutation on alteration of ion permeability of ORF3a, proposed in this study brings mechanistic insights into variant consequences on viral membrane proteins of SARS-CoV-2, which can be utilized for the development of novel therapeutics to treat COVID-19 and other coronavirus diseases., Competing Interests: Declaration of competing interest The authors declare that there are no conflicts of interest., (Copyright © 2023 Elsevier B.V. All rights reserved.)
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- 2024
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31. Molecular docking and MD simulations reveal protease inhibitors block the catalytic residues in Prp8 intein of Aspergillus fumigatus: a potential target for antimycotics.
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Panda S, Rout M, Mishra S, Turuk J, Pati S, and Dehury B
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Resistance to azoles and amphotericin B especially in Aspergillus fumigatus is a growing concern towards the treatment of invasive fungal infection. At this critical juncture, intein splicing would be a productive, and innovative target to establish therapies against resistant strains. Intein splicing is the central event for the activation of host protein, essential for the growth and survival of various microorganisms including A. fumigatus . The splicing process is a four-step protease-like nucleophilic cascade. Thus, we hypothesise that protease inhibitors would successfully halt intein splicing and potentially restrict the growth of the aforementioned pathogen. Using Rosetta Fold and molecular dynamics simulations, we modelled Prp8 intein structure; resembling classic intein fold with horse shoe shaped splicing domain. To fully comprehend the active site of Afu Prp8 intein, C1, T62, H65, H818, N819 from intein sequences and S820, the first C-extein residue are selected. Molecular docking shows that two FDA-approved drugs, i.e. Lufotrelvir and Remdesivir triphosphate efficiently interact with Prp8 intein from the assortment of 212 protease inhibitors. MD simulation portrayed that Prp8 undergoes conformational change upon ligand binding, and inferred the molecular recognition and stability of the docked complexes. Per-residue decomposition analysis confirms the importance of F: block R802, V803, and Q807 binding pocket in intein splicing domain towards recognition of inhibitors, along with active site residues through strong hydrogen bonds and hydrophobic contacts. However, in vitro and in vivo assays are required to confirm the inhibitory action on Prp8 intein splicing; which may pave the way for the development of new antifungals for A. fumigatus .Communicated by Ramaswamy H. Sarma.
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- 2023
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32. Assessing the prediction of type 2 diabetes risk using polygenic and clinical risk scores in South Asian study populations.
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Rout M, Wander GS, Ralhan S, Singh JR, Aston CE, Blackett PR, Chernausek S, and Sanghera DK
- Abstract
Background: Genome-wide polygenic risk scores (PRS) have shown high specificity and sensitivity in predicting type 2 diabetes (T2D) risk in Europeans. However, the PRS-driven information and its clinical significance in non-Europeans are underrepresented. We examined the predictive efficacy and transferability of PRS models using variant information derived from genome-wide studies of Asian Indians (AIs) (PRS
AI ) and Europeans (PRSEU ) using 13,974 AI individuals., Methods: Weighted PRS models were constructed and analyzed on 4602 individuals from the Asian Indian Diabetes Heart Study/Sikh Diabetes Study (AIDHS/SDS) as discovery/training and test/validation datasets. The results were further replicated in 9372 South Asian individuals from UK Biobank (UKBB). We also assessed the performance of each PRS model by combining data of the clinical risk score (CRS)., Results: Both genetic models (PRSAI and PRSEU ) successfully predicted the T2D risk. However, the PRSAI revealed 13.2% odds ratio (OR) 1.80 [95% confidence interval (CI) 1.63-1.97; p = 1.6 × 10-152 ] and 12.2% OR 1.38 (95% CI 1.30-1.46; p = 7.1 × 10-237 ) superior performance in AIDHS/SDS and UKBB validation sets, respectively. Comparing individuals of extreme PRS (ninth decile) with the average PRS (fifth decile), PRSAI showed about two-fold OR 20.73 (95% CI 10.27-41.83; p = 2.7 × 10-17 ) and 1.4-fold OR 3.19 (95% CI 2.51-4.06; p = 4.8 × 10-21 ) higher predictability to identify subgroups with higher genetic risk than the PRSEU . Combining PRS and CRS improved the area under the curve from 0.74 to 0.79 in PRSAI and 0.72 to 0.75 in PRSEU ., Conclusion: Our data suggest the need for extending genetic and clinical studies in varied ethnic groups to exploit the full clinical potential of PRS as a risk prediction tool in diverse study populations., Competing Interests: The authors declare that there is no conflict of interest., (© The Author(s), 2023.)- Published
- 2023
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33. Estimation of foot-and-mouth disease virus sero-prevalence rates using novel computational approach for the susceptible bovine population in India during the period 2008-2021.
- Author
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Das S, Pal S, Rautaray SS, Mohapatra JK, Subramaniam S, Rout M, Rai SN, and Singh RP
- Subjects
- Cattle, Animals, Prevalence, Antibodies, Viral, Disease Outbreaks veterinary, India epidemiology, Foot-and-Mouth Disease Virus, Cattle Diseases epidemiology, Cattle Diseases prevention & control, Foot-and-Mouth Disease epidemiology, Foot-and-Mouth Disease prevention & control
- Abstract
Foot-and-mouth disease (FMD) is a severe contagious viral disease of cloven-hoofed animals. In India, a vaccination-based official FMD control programme was started, which got expanded progressively to cover entire country in 2019. The serological tests are used to determine non-structural protein based sero-prevalence rates for properly implementing and assessing the control programme. Since 2008, reporting of the FMD sero-surveillance was limited to the serum sample-based serological test results without going for population-level estimation due to lack of proper statistical methodology. Thus, we present a computational approach for estimating the sero-prevalence rates at the state and national levels. Based on the reported approach, a web-application ( https://nifmd-bbf.icar.gov.in/FMDSeroSurv ) and an R software package ( https://github.com/sam-dfmd/FMDSeroSurv ) have been developed. The presented computational techniques are applied to the FMD sero-surveillance data during 2008-2021 to get the status of virus circulation in India under a strict vaccination policy. Furthermore, through various structural equation models, we attempt to establish a link between India's estimated sero-prevalence rate and field FMD outbreaks. Our results indicate that the current sero-prevalence rates are significantly associated with previous field outbreaks up to 2 years. Besides, we observe downward trends in sero-prevalence and outbreaks over the years, specifically after 2013, which indicate the effectiveness of various measures implemented under the FMD control programme. The findings of the study may help researchers and policymakers to track virus infection and identification of potential disease-free zones through vaccination., (© 2023. The Author(s).)
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- 2023
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34. MagMet: A fully automated web server for targeted nuclear magnetic resonance metabolomics of plasma and serum.
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Rout M, Lipfert M, Lee BL, Berjanskii M, Assempour N, Fresno RV, Cayuela AS, Dong Y, Johnson M, Shahin H, Gautam V, Sajed T, Oler E, Peters H, Mandal R, and Wishart DS
- Subjects
- Humans, Magnetic Resonance Spectroscopy methods, Serum, Algorithms, Magnetic Resonance Imaging, Metabolomics methods
- Abstract
Nuclear magnetic resonance (NMR) spectral analysis of biofluids can be a time-consuming process, requiring the expertise of a trained operator. With NMR becoming increasingly popular in the field of metabolomics, there is a growing need to change this paradigm and to automate the process. Here we introduce MagMet, an online web server, that automates the processing and quantification of 1D
1 H NMR spectra from biofluids-specifically, human serum/plasma metabolites, including those associated with inborn errors of metabolism (IEM). MagMet uses a highly efficient data processing procedure that performs automatic Fourier Transformation, phase correction, baseline optimization, chemical shift referencing, water signal removal, and peak picking/peak alignment. MagMet then uses the peak positions, linewidth information, and J-couplings from its own specially prepared standard metabolite reference spectral NMR library of 85 serum/plasma compounds to identify and quantify compounds from experimentally acquired NMR spectra of serum/plasma. MagMet employs linewidth adjustment for more consistent quantification of metabolites from higher field instruments and incorporates a highly efficient data processing procedure for more rapid and accurate detection and quantification of metabolites. This optimized algorithm allows the MagMet webserver to quickly detect and quantify 58 serum/plasma metabolites in 2.6 min per spectrum (when processing a dataset of 50-100 spectra). MagMet's performance was also assessed using spectra collected from defined mixtures (simulating other biofluids), with >100 previously measured plasma spectra, and from spiked serum/plasma samples simulating known IEMs. In all cases, MagMet performed with precision and accuracy matching the performance of human spectral profiling experts. MagMet is available at http://magmet.ca., (© 2023 The Authors. Magnetic Resonance in Chemistry published by John Wiley & Sons Ltd.)- Published
- 2023
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35. A reverse transcription-multiplex PCR strategy devised for concomitant detection and differentiation of foot and mouth disease virus serotypes O, A and Asia 1 in India.
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Mohapatra JK, Rout M, Subramaniam S, Giri P, Dahiya SS, Rautaray SS, Biswal JK, Sahoo NR, and Singh RP
- Subjects
- Animals, Serogroup, Reverse Transcription, Multiplex Polymerase Chain Reaction, Serotyping, Sensitivity and Specificity, India, Cell Differentiation, Foot-and-Mouth Disease Virus, Foot-and-Mouth Disease diagnosis
- Abstract
Serotype identification occupies the central part of foot and mouth disease (FMD) diagnosis workflow and vaccination decision tree. In this study, a reverse transcription-multiplex PCR (RT-mPCR) strategy wherein three assays with unique combinations of serotype specific primers targeting the VP1 region was developed to differentiate FMD virus serotypes O, A and Asia 1 based on differential size of the PCR amplicons on agarose gel. Their diagnostic performance relative to the mPCR assay in use in India was evaluated on 169 clinical samples and 210 cell culture grown virus isolates. The relative diagnostic sensitivity was found to be 99.69%, 98.78% and 99.08% for primer combinations 1, 2 and 3, respectively. These assays proved their worth by detecting serotype in three FMD suspected specimens that went undiagnosed in the existing mPCR and also by identifying multiple serotypes in the same sample. Their detection limits varied from log
10 2 to log10 4 viral RNA dilution and from 100 to 0.1 TCID50 virus depending on the serotype. The validated novel mPCR assays show promise to be included in the routine diagnostic tool-box to augment the efficiency of diagnosis of FMD virus serotypes that display extreme genetic diversity and a tendency of transboundary dispersal., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier B.V. All rights reserved.)- Published
- 2023
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36. Production and characterization of monoclonal antibodies against foot-and-mouth disease virus serotype O and development of a sandwich ELISA for virus antigen detection.
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Mallick S, Singh RP, Biswal JK, Mohapatra JK, Rout M, Samanta R, Khulape SA, and Ranjan R
- Subjects
- Mice, Animals, Rabbits, Antibodies, Monoclonal, Serogroup, O Antigens, Enzyme-Linked Immunosorbent Assay veterinary, Antibodies, Viral, Foot-and-Mouth Disease Virus, Foot-and-Mouth Disease diagnosis, Vaccines
- Abstract
Foot-and-mouth disease (FMD) is endemic in India with a majority of outbreaks caused by FMD virus (FMDV) serotype O. In the present study a panel of eight (2F9, 2G10, 3B9, 3H5, 4C8, 4D6, 4G10 and 5B6) mouse monoclonal antibodies (MAbs) were developed against FMDV serotype O Indian vaccine strain, O/IND/R2/75 via hybridoma systems. The MAbs generated were FMDV/O specific without cross-reactivity against FMDV type A and Asia 1. All the MAbs were identified as IgG1/kappa type. Out of eight, three MAbs (3B9, 3H5 and 4G10) demonstrated virus neutralizing activity. The reactivity of all MAbs increased with heat treated (@56
0 C) serotype O antigen compared to untreated antigen in sandwich ELISA indicating that their binding epitopes are linear. Six MAbs (except 2F9 and 4D6) reacted with recombinant P1 protein of homologous virus in an indirect ELISA among which only MAb 3B9 bound to VP1. MAb profiling of 37 serotype O field viruses isolated between the years 1962 and 2021 demonstrated antigenic similarity between field isolates and reference vaccine strain. MAbs 5B6 and 4C8 consistently reacted with all 37 isolates. In indirect immunofluorescence assay MAb 5B6 bound well with FMDV/O antigen. Finally, a sandwich ELISA was successfully developed using rabbit polyclonal anti-FMDV/O serum and MAb 5B6 for detection of FMDV/O antigen in clinical samples (n = 649). The new assay exhibited 100% and 98.89% diagnostic sensitivity and specificity respectively compared to traditional polyclonal antibody-based sandwich ELISA suggesting that the MAb-based ELISA developed here could be an effective method for detection of FMDV serotype O., (© 2023. The Author(s), under exclusive licence to Springer Nature B.V.)- Published
- 2023
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37. Automated identification and quantification of metabolites in human fecal extracts by nuclear magnetic resonance spectroscopy.
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Lee BL, Rout M, Mandal R, and Wishart DS
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- Humans, Magnetic Resonance Spectroscopy methods, Magnetic Resonance Imaging, Metabolomics methods, Software
- Abstract
We report the development of a software program, called MagMet-F, that automates the processing and quantification of 1D
1 H NMR of human fecal extracts. To optimize the program, we identified 82 potential fecal metabolites using 1D1 H NMR of six human fecal extracts using manual profiling and a literature review of known fecal metabolites. We acquired pure versions of those metabolites and then acquired their 1D1 H NMR spectra at 700 MHz to generate a fecal metabolite spectral library for MagMet-F. The fitting of these metabolites by MagMet-F was iteratively optimized to replicate manual profiling. We validated MagMet-F's automated profiling using a test set of six fecal extracts. It correctly identified 80% of the compounds and quantified those within <20% of the values determined by manual profiling using Chenomx. We also compared MagMet-F's profiling performance to two other open-access NMR profiling tools, Bayesil and Batman. MagMet-F outperformed both. Bayesil repeatedly overestimated metabolite concentrations by 10% to 40% while Batman was unable to properly quantify any compounds and took 10-20× longer. We have implemented MagMet-F as a freely accessible web server to enable automated, fast and convenient 1D1 H NMR spectral profiling of fecal samples. MagMet-F is available at https://www.magmet.ca., (© 2023 The Authors. Magnetic Resonance in Chemistry published by John Wiley & Sons Ltd.)- Published
- 2023
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38. Therapeutic Management of Giant Uterine Fibroid: A Case Report.
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Rout M, Dave A, and Bakshi SS
- Abstract
The most common benign tumor that affects female reproductive system is a uterine fibroid or leiomyomas, especially during their reproductive years. The prevalence is around 20-40% women in the age group of 14-45 years. The following case report proffers the diagnosis and management of a female diagnosed with uterine fibroids. In this case a 45-year-old woman presented to the tertiary rural hospital with an unusually large distended abdomen which on examination and imaging revealed a big fibroid growing inside the uterus. The case highlights the significance of a collaborative approach involving gynecologists, interventional radiologists, and surgeons. Their combined expertise provides patients with various treatment options: medical management, minimally invasive procedures, and surgical interventions. During the decision-making process, factors such as the patient's age, desire for fertility preservation, and impact of fibroids on her quality of life are taken into account. This particular case showcases successful pre-hysterectomy uterine artery embolization of large uterine leiomyoma while emphasizing the importance of personalized care and shared decision making for optimal patient outcomes., Competing Interests: The authors have declared that no competing interests exist., (Copyright © 2023, Rout et al.)
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- 2023
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39. Illuminating the structural basis of human neurokinin 1 receptor (NK1R) antagonism through classical all-atoms molecular dynamics simulations.
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Mishra S, Rout M, Singh MK, Dehury B, and Pati S
- Subjects
- Humans, Molecular Dynamics Simulation, Lipids, Neurokinin-1 Receptor Antagonists pharmacology, Receptors, Neurokinin-1 metabolism
- Abstract
Advances in structural biology have bestowed insights into the pleiotropic effects of neurokinin 1 receptors (NK1R) in diverse patho-physiological processes, thereby highlighting the potential therapeutic value of antagonists directed against NK1R. Herein, we investigate the mode of antagonist recognition to discern the obscure atomic facets germane for the function and molecular determinants of NK1R. To commence discernment of potent antagonists and the conformational changes in NK1R, induced upon antagonist binding, state-of-the-art classical all-atoms molecular dynamics (MD) simulations in lipid mimetic bilayers have been utilized. MD simulations of structural ensembles reveals the involvement of TM5 and TM6 in tight anchoring of antagonists through a network of interhelical hydrogen-bonds, while, the extracellular loop 2 (ECL2) governs the overall size and nature of the pocket, thereby modulating NK1R. Consistent comparison between experiments and MD simulation results discerns the predominant role of TM3, TM4, and TM6 in lipid-NK1R interaction. Correlation between hydrophobic index and helicity of TM domains elucidates their importance in maintaining the structural stability in addition to regulating NK1R antagonism. Taken together, we anticipate that our computational study marks a comprehensive structural basis of NK1R antagonism in lipid bilayers, which may facilitate designing of new therapeutics against associated diseases targeting human neurokinin receptors., (© 2023 Wiley Periodicals LLC.)
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- 2023
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40. Discovery and validation of circulating stroke metabolites by NMR-based analyses using patients from the MISS and UK Biobank.
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Rout M, Vaughan A, Blair A, Stavrakis S, Sidorov EV, and Sanghera DK
- Subjects
- Humans, Biological Specimen Banks, Metabolome, Magnetic Resonance Spectroscopy methods, Metabolomics methods, Biomarkers, United Kingdom epidemiology, Stroke, Ischemic Stroke
- Abstract
Background: Stroke is a significant health issue in the United States, and identifying biomarkers for the prevention and functional recovery after an acute stroke remains the highest priority. This study aims to identify circulating metabolite signatures that may be associated with stroke pathophysiology by performing discovery and validation studies., Methods: We performed targeted metabolomics profiling of 420 participants of the discovery dataset of Metabolome in an Ischemic Stroke Study (MISS) using high-throughput nuclear magnetic resonance (NMR) spectroscopy. A validation study of significantly altered metabolites was conducted using an independent cohort of 117,988 participants from the UK Biobank, whose metabolomics profiles were generated using the same NMR technology., Results and Conclusion: Our study identified 16 metabolites to be significantly perturbed during acute stroke. Amino acid phenylalanine was significantly increased, while glutamine and histidine were significantly lowered in stroke. Serum levels of apolipoprotein A-1, HDL particles, small HDL particles, essential fatty acids, and phosphatidylcholine were reduced, while ketone bodies like 3-hydroxybutyrate and acetoacetate were markedly increased in stroke. Based on the robust validation in a large independent UK Biobank dataset, some of these analytes may become clinically meaningful biomarkers to predict or prevent stroke in humans., Competing Interests: Declaration of competing interest We declare that there is no conflict of interest that could be perceived as prejudicing the impartiality of the research reported., (Copyright © 2023. Published by Elsevier Ltd.)
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- 2023
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41. Difference in acute and chronic stage ischemic stroke metabolic markers with controls.
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Sidorov EV, Rout M, Xu C, Jordan L, Fields E, Apple B, Smith K, Gordon D, Chainakul J, and Sanghera DK
- Subjects
- Humans, Longitudinal Studies, Pilot Projects, Alanine, Biomarkers, Ischemic Stroke diagnosis, Stroke diagnostic imaging
- Abstract
Background: Acute Ischemic Stroke (AIS), a major cause of disability, was previously associated with multiple metabolomic changes, but many findings were contradictory. Case-control and longitudinal study designs could have played a role in that. To clarify metabolomic changes, we performed a simultaneous comparison of ischemic stroke metabolome in acute, chronic stages of stroke and controls., Methods: Through the nuclear magnetic resonance (NMR) platform, we evaluated 271 serum metabolites from a cohort of 297 AIS patients in acute and chronic stages and 159 controls. We used Sparse Partial Least Squares-Discriminant analysis (sPLS-DA) to evaluate group disparity; multivariate regression to compare metabolome in acute, chronic stages of stroke and controls; and mixed regression to compare metabolome acute and chronic stages of stroke. We applied false discovery rate (FDR) to our calculations., Results: The sPLS-DA revealed separation of the metabolome in acute, chronic stages of stroke and controls. Regression analysis identified 38 altered metabolites. Ketones, branched-chain amino acids (BCAAs), energy, and inflammatory compounds were mostly elevated, while alanine and glutamine were decreased in the acute stage. These metabolites declined/increased in the chronic stage, often to the same levels as in controls. Levels of fatty acids, phosphatidylcholines, phosphoglycerides, and sphingomyelins did not change between acute and chronic stages, but were different comparing to controls., Conclusion: Our pilot study identified metabolites associated with acute stage of ischemic stroke and those that are altered in stroke patients comparing to controls regardless of stroke acuity. Future investigation in a larger independent cohort is needed to validate these findings., Competing Interests: Declaration of Competing Interest Authors declare the following grant support (NIH (R01 DK118427), Presbyterian Health Foundation Team Science Award, College of Medicine Alumni Association Award (COMAA), and the Leinbach Seed Grant), but no other interests., (Copyright © 2023. Published by Elsevier Inc.)
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- 2023
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42. Exploiting the potential of natural polyphenols as antivirals against monkeypox envelope protein F13 using machine learning and all-atoms MD simulations.
- Author
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Rout M, Mishra S, Dey S, Singh MK, Dehury B, and Pati S
- Subjects
- Humans, Antiviral Agents pharmacology, Antiviral Agents therapeutic use, Antiviral Agents chemistry, Molecular Dynamics Simulation, Polyphenols, Pandemics, Molecular Docking Simulation, Mpox (monkeypox), COVID-19
- Abstract
The re-emergence of monkeypox (MPX), in the era of COVID-19 pandemic is a new global menace. Regardless of its leniency, there are chances of MPX expediting severe health deterioration. The role of envelope protein, F13 as a critical component for production of extracellular viral particles makes it a crucial drug target. Polyphenols, exhibiting antiviral properties have been acclaimed as an effective alternative to the traditional treatment methods for management of viral diseases. To facilitate the development of potent MPX specific therapeutics, herein, we have employed state-of-the-art machine learning techniques to predict a highly accurate 3-dimensional structure of F13 as well as identify binding hotspots on the protein surface. Additionally, we have effectuated high-throughput virtual screening methodology on 57 potent natural polyphenols having antiviral activities followed by all-atoms molecular dynamics (MD) simulations, to substantiate the mode of interaction of F13 protein and polyphenol complexes. The structure-based virtual screening based on Glide SP, XP and MM/GBSA scores enables the selection of six potent polyphenols having higher binding affinity towards F13. Non-bonded contact analysis, of pre- and post- MD complexes propound the critical role of Glu143, Asp134, Asn345, Ser321 and Tyr320 residues in polyphenol recognition, which is well supported by per-residue decomposition analysis. Close-observation of the structural ensembles from MD suggests that the binding groove of F13 is mostly hydrophobic in nature. Taken together, this structure-based analysis from our study provides a lead on Myricetin, and Demethoxycurcumin, which may act as potent inhibitors of F13. In conclusion, our study provides new insights into the molecular recognition and dynamics of F13-polyphenol bound states, offering new promises for development of antivirals to combat monkeypox. However, further in vitro and in vivo experiments are necessary to validate these results., Competing Interests: Declaration of competing interest The authors declare that there are no conflicts of interest., (Copyright © 2023 Elsevier Ltd. All rights reserved.)
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- 2023
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43. Emergence of a novel genetic lineage 'A/ASIA/G-18/2019' of foot and mouth disease virus serotype A in India: A challenge to reckon with.
- Author
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Mohapatra JK, Dahiya SS, Subramaniam S, Rout M, Biswal JK, Giri P, Nayak V, and Singh RP
- Subjects
- Animals, Serogroup, Antigens, Viral, India epidemiology, Phylogeny, Foot-and-Mouth Disease Virus genetics, Foot-and-Mouth Disease
- Abstract
Foot and mouth disease (FMD) has engendered large scale socioeconomic crises on numerous occasions owing to its extreme contagiousness, transboundary nature, complicated epidemiology, negative impact on productivity, trade embargo, and need for intensive surveillance and expensive control measures. Emerging FMD virus variants have been predicted to have originated and spread from endemic Pool 2, native to South Asia, to other parts of the globe. In this study, 26 Indian serotype A isolates sampled between the year 2015 and 2022 were sequenced for the VP1 region. BLAST and maximum likelihood phylogeny suggest emergence of a novel genetic group within genotype 18, named here as 'A/ASIA/G-18/2019' lineage, that is restricted so far only to India and its eastern neighbour, Bangladesh. The lineage subsequent to its first appearance in 2019 seems to have displaced all other prevalent strains, in support of the phenomenon of 'genotype/lineage turnover'. It has diversified into two distinct sub-clusters, reflecting a phase of active evolution. The rate of evolution of the VP1 region for the Indian serotype A dataset was estimated to be 6.747 × 10
-3 substitutions/site/year. India is implementing a vaccination centric FMD control programme. The novel lineage showed good antigenic match with the proposed vaccine candidate A IND 27/2011 when tested in virus neutralization test, while the existing vaccine strain A IND 40/2000 showed homology with only 31% of the isolates. Therefore, in order to combat this challenge of antigenic divergence, A IND 27/2011 could be the preferred strain in the Indian vaccine formulations., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financialinterestsor personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023. Published by Elsevier B.V.)- Published
- 2023
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44. Foot-and-Mouth Disease Virus Serotype O Exhibits Phenomenal Genetic Lineage Diversity in India during 2018-2022.
- Author
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Dahiya SS, Subramaniam S, Mohapatra JK, Rout M, Biswal JK, Giri P, Nayak V, and Singh RP
- Subjects
- Animals, Serogroup, Phylogeny, Disease Outbreaks prevention & control, India epidemiology, Foot-and-Mouth Disease Virus, Foot-and-Mouth Disease
- Abstract
In India, widespread foot-and-mouth disease (FMD) outbreaks occurred in 2021. The objective of this study was to identify genetic lineages and evaluate the antigenic relationships of FMD virus (FMDV) isolates gathered from outbreaks reported between 2019 and 2022. Our study shows that the lineages O/ME-SA/Ind2001e and the O/ME-SA/Cluster-2018 were both responsible for the FMD outbreaks on an epidemic scale during 2021. This observation is in contrast to earlier findings that suggested epidemic-scale FMD outbreaks in India are often connected to a single genetic lineage. Additionally, we report here the identification of the O/ME-SA/PanAsia-2/ANT10 sub-lineage in India for the first time, which was connected to two intermittent outbreaks in Jammu and Kashmir. The current study demonstrates that the O/ME-SA/ind2001e lineage has a strong presence outside of the Indian subcontinent. Furthermore, the O/ME-SA/Cluster-2018 was observed to have a wider geographic distribution than previously, and like the O/ME-SA/Ind2001d and O/ME-SA/Ind2001e lineages in the past, it may eventually spread outside of its geographic niche. For O/ME-SA/Ind2001e and O/ME-SA/Cluster-2018, the predicted substitution rate for the VP1 region was 6.737 × 10
-3 and 8.257 × 10-3 nt substitutions per site per year, respectively. The time of the most recent common ancestor of the O/ME-SA/Ind2001e and O/ME-SA/Cluster-2018 strains suggests that the viruses possibly emerged during 2003-2011 and 2009-2017, respectively. Recent sightings of the O/ME-SA/PanAsia2/ANT10 virus in India and the O/ME-SA/Ind2001e virus in Pakistan point to possible cross-border transit of the viruses. The results of a two-dimensional viral neutralization test revealed that all of the field isolates were antigenically matched to the currently used Indian vaccine strain O INDR2/1975. These results suggest that the serotype O vaccine strain can protect against outbreaks brought on by all three circulating lineages.- Published
- 2023
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45. Development of TaqMan Probe-Based One-Step RT-qPCR Assay Targeting 2B-NSP Coding Region for Diagnosis of Foot-and-Mouth Disease in India.
- Author
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Biswal JK, Ranjan R, Mohapatra JK, Rout M, Joshi HR, and Singh RP
- Subjects
- Animals, Sensitivity and Specificity, Serogroup, Multiplex Polymerase Chain Reaction, Foot-and-Mouth Disease diagnosis, Foot-and-Mouth Disease Virus genetics
- Abstract
A one-step TaqMan probe-based RT-qPCR assay in the duplex format simultaneously targeting FMD Virus (FMDV) 2B NSP-coding region and 18S rRNA housekeeping gene was developed and evaluated. The duplex RT-qPCR assay specifically detected FMDV genome in both infected cell culture suspensions and a variety of clinical samples such as FMD-affected tongue/feet epithelium, oral/nasal swabs, milk and oro-pharyngeal fluids. The RT-qPCR assay was found to be highly sensitive, since the assay was 10
5 -fold more sensitive than the traditional FMDV detecting antigen-ELISA (Ag-ELISA) and 102 -fold better sensitive than both virus isolation and agarose gel-based RT-multiplex PCR. In addition, the assay could detect up to 100 copies of FMDV genome per reaction. In the epithelial samples (n = 582) collected from the FMD-affected animals, the diagnostic sensitivity was 100% (95% CI 99-100%). Similarly, all the FMDV-negative samples (n = 65) tested were confirmed negative by the new RT-qPCR assay, corresponding to 100% diagnostic specificity (95% CI = 94-100%). Further, the duplex RT-qPCR assay proved to be robust, showing an inter-assay co-efficient of variations ranging from 1.4 to 3.56% for FMDV-2B gene target, and from 2 to 4.12% for 18S rRNA gene target. While analyzing FMDV-infected cell culture suspension, a fairly strong positive correlation (correlation coefficient = 0.85) was observed between 2B-based RT-qPCR and WOAH-approved 5'UTR RT-qPCR assays. Therefore, the one-step RT-qPCR assay developed here with an internal control could be used for rapid, effective, and reliable detection of FMDV in pan-serotypic manner, and has the potential for routine diagnosis of FMDV in high throughput manner., (© 2023. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)- Published
- 2023
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46. Drug repurposing for Mpox: Discovery of small molecules as potential inhibitors against DNA-dependent RNA polymerase using molecular modeling approach.
- Author
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Dutt M, Kumar A, Rout M, Dehury B, Martinez G, Ndishimye P, Kelvin AA, and Kelvin DJ
- Subjects
- Humans, DNA-Directed RNA Polymerases, Molecular Dynamics Simulation, Antiviral Agents pharmacology, Antiviral Agents chemistry, Molecular Docking Simulation, Drug Repositioning, Mpox (monkeypox)
- Abstract
Mpox (formerly Monkeypox), a zoonotic illness caused by the Mpox virus, belongs to the Orthopoxvirus genus in the family Poxviridae. To design and develop effective antiviral therapeutics against DNA viruses, the DNA-dependent RNA polymerase (DdRp) of poxviruses has emerged as a promising drug target. In the present study, we modeled the three-dimensional (3D) structure of DdRp using a template-based homology approach. After modeling, virtual screening was performed to probe the molecular interactions between 1755 Food and Drug Administration-approved small molecule drugs (≤500 molecular weight) and the DdRp of Mpox. Based on the binding affinity and molecular interaction patterns, five drugs, lumacaftor (-11.7 kcal/mol), conivaptan (-11.7 kcal/mol), betulinic acid (-11.6 kcal/mol), fluspirilene (-11.3 kcal/mol), and imatinib (-11.2 kcal/mol), have been ranked as the top drug compounds interacting with Mpox DdRp. Complexes of these shortlisted drugs with DdRp were further evaluated using state-of-the-art all-atoms molecular dynamics (MD) simulations on 200 nanoseconds followed by principal component analysis (PCA). MD simulations and PCA results revealed highly stable interactions of these small drugs with DdRp. After due validation in wet-lab using available in vitro and in vivo experiments, these repurposed drugs can be further utilized for the treatment of contagious Mpox virus. The outcome of this study may establish a solid foundation to screen repurposed and natural compounds as potential antiviral therapeutics against different highly pathogenic viruses., (© 2023 The Authors. Journal of Cellular Biochemistry published by Wiley Periodicals LLC.)
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- 2023
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47. Improving the hole picture: towards a consensus on the mechanism of nuclear transport.
- Author
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Cowburn D and Rout M
- Subjects
- Active Transport, Cell Nucleus, Consensus, Cell Nucleus metabolism, Nuclear Pore Complex Proteins metabolism, Nuclear Pore metabolism
- Abstract
Nuclear pore complexes (NPCs) mediate the exchange of materials between the nucleoplasm and cytoplasm, playing a key role in the separation of nucleic acids and proteins into their required compartments. The static structure of the NPC is relatively well defined by recent cryo-EM and other studies. The functional roles of dynamic components in the pore of the NPC, phenylalanyl-glycyl (FG) repeat rich nucleoporins, is less clear because of our limited understanding of highly dynamic protein systems. These proteins form a 'restrained concentrate' which interacts with and concentrates nuclear transport factors (NTRs) to provide facilitated nucleocytoplasmic transport of cargoes. Very rapid on- and off-rates among FG repeats and NTRs supports extremely fast facilitated transport, close to the rate of macromolecular diffusion in cytoplasm, while complexes without specific interactions are entropically excluded, though details on several aspects of the transport mechanism and FG repeat behaviors remain to be resolved. However, as discussed here, new technical approaches combined with more advanced modeling methods will likely provide an improved dynamic description of NPC transport, potentially at the atomic level in the near future. Such advances are likely to be of major benefit in comprehending the roles the malfunctioning NPC plays in cancer, ageing, viral diseases, and neurodegeneration., (© 2023 The Author(s).)
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- 2023
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48. Depicting protein structures as schematics.
- Author
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Markert J, Luger K, Lee H, Hu H, Taylor NMI, Fernandez-Martinez J, Rout M, and Alder N
- Subjects
- Proteins chemistry, Protein Conformation
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- 2023
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49. Comparison of Acute and Chronic Stage Ischemic Stroke Metabolome with Controls.
- Author
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Sidorov EV, Rout M, Xu C, Larsen J, Fields E, Apple B, Smith K, Gordon D, Chainakul J, and Sanghera D
- Abstract
Background: Acute Ischemic Stroke (AIS), a major cause of disability, was previously associated with multiple metabolomic changes, but many findings were contradictory. Case-control and longitudinal study designs could have played a role in that. To clarify metabolomic changes, we performed a simultaneous comparison of ischemic stroke metabolome in acute, chronic stages of stroke and controls., Methods: Through the nuclear magnetic resonance (NMR) platform, we evaluated 271 serum metabolites from a cohort of 297 AIS patients in acute and chronic stages and 159 controls. We used Sparse Partial Least Squares-Discriminant analysis (sPLS-DA) to evaluate group disparity; multivariate regression to compare metabolome in acute, chronic stages of stroke and controls; and mixed regression to compare metabolome acute and chronic stages of stroke. We applied false discovery rate (FDR) to our calculations., Results: The sPLS-DA revealed separation of the metabolome in acute, chronic stages of stroke and controls. Regression analysis identified 38 altered metabolites. Ketone bodies, branched-chain amino acids (BCAAs), energy, and inflammatory compounds were elevated in the acute stage, but declined in the chronic stage, often to the same levels as in controls. Levels of other amino acids, phosphatidylcholines, phosphoglycerides, and sphingomyelins mainly did not change between acute and chronic stages, but was different comparing to controls., Conclusion: Our pilot study identified metabolites associated with acute stage of ischemic stroke and those that are altered in stroke patients comparing to controls regardless of stroke acuity. Future investigation in a larger independent cohort is needed to validate these findings., Competing Interests: Disclosures: The authors have nothing to disclose
- Published
- 2023
- Full Text
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50. An immunoinformatic approach towards development of a potent and effective multi-epitope vaccine against monkeypox virus (MPXV).
- Author
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Mishra S, Rout M, Panda S, Singh SK, Sinha R, Dehury B, and Pati S
- Subjects
- Animals, Humans, Toll-Like Receptor 4, Pandemics, Epitopes, B-Lymphocyte, Computational Biology, Molecular Docking Simulation, Epitopes, T-Lymphocyte, Vaccines, Subunit, Monkeypox virus, COVID-19
- Abstract
Monkeypox is a viral zoonotic disease, often transmitted to humans from animals. While the whole world is haggling with the COVID-19 pandemic, the emergence of the monkeypox virus (MPXV) arose as a new challenge to mankind. Till date, numerous cases related to the MPXV have been reported in several countries across the globe, but, its momentary distribution in the current time has left everyone in fright with increasing mortality and limited clinically approved treatments. Therefore, it is of immense importance to develop a potent and highly effective vaccine capable of inducing desired immunogenic responses against the highly contagious MPXV. Herein, using various immunoinformatic and computational biology tools, we made an attempt to develop a multi-epitope vaccine construct against the MPXV which is antigenic, non-allergen and non-toxic in nature and capable of exhibiting immunogenic behavior. The sequence of vaccine construct was designed using the proposed 4 MHC-I, 3 MHC-II and 4 B-cell epitopes linked with suitable adjuvant and linkers. The modeled structure of the vaccine construct was used to assess its interaction with the Toll-like Receptor 4 (TLR4) using ClusPro and HADDOCK. All-atoms molecular dynamics simulation of the MPXV vaccine construct-TLR4 complex followed by a high level of gene expression of the construct within the bacterial system affirmed its stability along with induction of immunogenic response within the host cell. Altogether, our immunoinformatic approach aid in the development of a stable chimeric vaccine construct against MPXV and needs further experimental validation for its immunological relevance and usefulness as a vaccine candidate.Communicated by Ramaswamy H. Sarma.
- Published
- 2023
- Full Text
- View/download PDF
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