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This study investigates how user demographics influence the linguistic characteristics and readability of the reviews and the performance of sentiment classification methods. Two restaurant review datasets representing consumers of distinct demographics, such as English language fluency levels, socio-culture, and geographic locations, are compared. It is observed that demographic dissimilarities affect various linguistic attributes, such as sentence structure and vocabulary usage of reviews. The Mann–Whitney U test indicates significant differences in these two sets of reviews on various linguistic aspects. Regarding the readability of the two corpora, no noticeable difference is perceived by Flesch Reading Ease (FRE) test. The discrepancy in the results of lexicon-based and machine learning-based sentiment classification methods in two corpora suggests that demography has an influential role in the performance of sentiment classifiers.

Within cells, cytoskeletal filaments are often arranged into loosely aligned bundles. These fibrous bundles are dense enough to exhibit a certain regularity and mean direction, however, their packing is not sufficient to impose a symmetry between—or specific shape on—individual filaments. This intermediate regularity is computationally difficult to handle because individual filaments have a certain directional freedom, however, the filament densities are not well segmented from each other (especially in the presence of noise, such as in cryo-electron tomography). In this paper, we develop a dynamic programming-based framework, Spaghetti Tracer, to characterizing the structural arrangement of filaments in the challenging 3D maps of subcellular components. Assuming that the tomogram can be rotated such that the filaments are oriented in a mean direction, the proposed framework first identifies local seed points for candidate filament segments, which are then grown from the seeds using a dynamic programming algorithm. We validate various algorithmic variations of our framework on simulated tomograms that closely mimic the noise and appearance of experimental maps. As we know the ground truth in the simulated tomograms, the statistical analysis consisting of precision, recall, and F1 scores allows us to optimize the performance of this new approach. We find that a bipyramidal accumulation scheme for path density is superior to straight-line accumulation. In addition, the multiplication of forward and backward path densities provides for an efficient filter that lifts the filament density above the noise level. Resulting from our tests is a robust method that can be expected to perform well (F1 scores 0.86–0.95) under experimental noise conditions.