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Plants exude a wide variety of compounds into the rhizosphere, modulating soil functioning and diversity. The number of studies investigating exudation has exponentially increased over the past decades. Yet, the high inter-study variability of the results is slowing down our understanding of root-soil interactions. This variability is partly due to the absence of harmonized methodologies to collect and characterize exudation. Here, we discuss how various experimental aspects influence exudation profiles by performing a literature review, and we suggest best practices for different experimental setups. We discuss state-of-the-art of spatially resolved exudate collection, collection in controlled versus field conditions and plant growth setups ranging from hydroponics to soil. We highlight the importance of preparing experimental blanks, in situ versus ex situ exudate collection, various collection media and timing of collection, exudate storage and processing and analytical considerations. We summarize best practices for experimental setup and reporting of parameters in an easily accessible table format to facilitate discussion of best practices in the field. An increased standardization in the field together with the systematic studies suggested will improve our knowledge of how plant exudation shapes interactions with organisms in soil., (© 2024 John Wiley & Sons Ltd.)

Acute kidney injury (AKI) is a frequent, severe complication of hematopoietic stem cell transplantation (HSCT) and is associated with an increased risk of morbidity and mortality. Recent advances in artificial intelligence (AI) and machine learning (ML) have showcased their proficiency in predicting AKI, projecting disease progression, and accurately identifying underlying etiologies. This review examines the central aspects of AKI post-HSCT, veno-occlusive disease (VOD) in HSCT recipients, discusses present-day applications of artificial intelligence in AKI, and introduces a proposed ML framework for the early detection of AKI risk.

Plants release a wealth of metabolites into the rhizosphere that can shape the composition and activity of microbial communities in response to environmental stress. The connection between rhizodeposition and rhizosphere microbiome succession has been suggested, particularly under environmental stress conditions, yet definitive evidence is scarce. In this study, we investigated the relationship between rhizosphere chemistry, microbiome dynamics, and abiotic stress in the bioenergy crop switchgrass grown in a marginal soil under nutrient-limited, moisture-limited, and nitrogen (N)-replete, phosphorus (P)-replete, and NP-replete conditions. We combined 16S rRNA amplicon sequencing and LC-MS/MS-based metabolomics to link rhizosphere microbial communities and metabolites. We identified significant changes in rhizosphere metabolite profiles in response to abiotic stress and linked them to changes in microbial communities using network analysis. N-limitation amplified the abundance of aromatic acids, pentoses, and their derivatives in the rhizosphere, and their enhanced availability was linked to the abundance of bacterial lineages from Acidobacteria, Verrucomicrobia, Planctomycetes, and Alphaproteobacteria. Conversely, N-amended conditions increased the availability of N-rich rhizosphere compounds, which coincided with proliferation of Actinobacteria. Treatments with contrasting N availability differed greatly in the abundance of potential keystone metabolites; serotonin and ectoine were particularly abundant in N-replete soils, while chlorogenic, cinnamic, and glucuronic acids were enriched in N-limited soils. Serotonin, the keystone metabolite we identified with the largest number of links to microbial taxa, significantly affected root architecture and growth of rhizosphere microorganisms, highlighting its potential to shape microbial community and mediate rhizosphere plant-microbe interactions., Competing Interests: Competing interests statement:The authors declare no competing interest.

Introduction: Moral courage manifests in acts intended to intervene to stop or redress witnessed moral norm violations, despite the risk of negative consequences for the intervener. We investigate moral courage in everyday life and ask what personality processes are involved. Based on an extended process model of moral courage, we derived hypotheses on cognitive and emotional processes that should facilitate or hinder intervention. Further, we identified candidate personality dispositions that should shape these processes and thereby predict who tends to intervene against others' norm violations and who does not., Methods: Using a quota-based sample of the German population (N = 1108), we conducted a personality assessment, followed by a 7-day experience sampling during which participants reported norm violations witnessed in their daily life as well as their cognitive, emotional, and behavioral reactions., Results: In total, 678 participants reported 1965 norm violations and intervened against 32% of them. Dispositional self-efficacy facilitated intervention by increasing a sense of efficacy when confronted with others' norm violations. Conversely, dispositional moral disengagement hindered intervention by reducing perceived own responsibility., Discussion: Our findings provide novel insights into the situations affording moral courage in everyday life, and the personality processes that uniquely guide this behavior., (© 2023 The Authors. Journal of Personality published by Wiley Periodicals LLC.)

Carbon sequestration to soils counteracts increasing CO2 levels in the atmosphere, and increases soil fertility. Efforts to increase soil carbon storage produced mixed results, due to the multifactorial nature of this process, and the lack of knowledge on molecular details on the interplay of plants, microbes, and soil physiochemical properties. This review outlines the carbon flow from the atmosphere into soils, and factors resulting in elevated or decreased carbon sequestration are outlined. Carbon partitioning within plants defines how much fixed carbon is allocated belowground, and plant and microbial respiration accounts for the significant amount of carbon lost. Carbon enters the soil in form of soluble and polymeric rhizodeposits, and as shoot and root litter. These different forms of carbon are immobilized in soils with varying efficiency as mineral-bound or particulate organic matter. Plant-derived carbon is further turned over by microbes in different soil layers. Microbial activity and substrate use is influenced by the type of carbon produced by plants (molecular weight, chemical class). Further, soil carbon formation is altered by root depth, growth strategy (perennial versus annual), and C/N ratio of rhizodeposits influence soil carbon formation. Current gaps of knowledge and future directions are highlighted., (Copyright 2023 J. Sasse. License: This work is licensed under a Creative Commons Attribution 4.0 International License.)

Root exudates shape the plant-soil interface, are involved in nutrient cycling and modulate interactions with soil organisms. Root exudates are dynamic and shaped by biological, environmental, and experimental conditions. Due to their wide diversity and low concentrations, accurate exudate profiles are challenging to determine, even more so in natural environments where other organisms are present, turning over plant-derived compounds and producing additional compounds themselves. The semihydroponic glass jar experimental system introduced here allows control over biological, environmental, and experimental factors. It allows the growth of various phylogenetically distinct plant species for up to several months with or without microbes, in a variety of different growth media. The glass-based design offers a low metabolite background for high sensitivity and low environmental impact as it can be reused. Exudates can be sampled nondestructively, and conditions can be altered over the course of an experiment if desired. The setup is compatible with mass spectrometry analytics and other downstream analytical procedures. In summary, we present a versatile growth system suited for sensitive root exudate analysis in a variety of conditions.

Introduction: Imprecise nutritional recommendations due to a lack of diagnostic test accuracy are a frequent problem for individuals with adverse reactions to foods but no precise diagnosis. Consequently, patients follow very broad and strict elimination diets to avoid uncontrolled symptoms such as diarrhoea and abdominal pain. Dietary limitations and the uncertainty of developing gastrointestinal symptoms after the inadvertent ingestion of food have been demonstrated to reduce the quality of life (QoL) of affected individuals and subsequently might increase the risk of malnutrition and intestinal dysbiosis. This trial aims to investigate the effects of a tailored diet based on the confocal laser endoscopy (CLE) examination result to limit the side effects of unspecific and broad elimination diets and to increase the patient's QoL., Methods and Analysis: The study is designed as a prospective, double-blind, monocentric, randomised and controlled trial conducted at the University Hospital of Schleswig-Holstein, Campus Lübeck, Germany. One hundred seventy-two patients with non-IgE-related food allergies and positive CLE results will be randomised to either a tailored diet or a standard fivefold elimination diet. The primary endpoints are the difference between the end and the start of the intervention in health-related QoL and the sum score of the severity of symptoms after 12 weeks. Key secondary endpoints are changes in the severity of symptoms, further QoL measurements, self-assessed state of health and number of days with a pathologically altered stool. Microbiome diversity and metabolome of stool, urine and blood will also be investigated. Safety endpoints are body composition, body mass index and adverse events., Ethics and Dissemination: The study protocol was accepted by the ethical committee of the University of Lübeck (AZ: 22-111) on 4 May2022. Results of the study will be published in peer-reviewed journals and presented at scientific meetings., Trial Registration Number: German Clinical Trials Register (DRKS00029323)., Competing Interests: Competing interests: None declared., (© Author(s) (or their employer(s)) 2023. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.)

Semantic interoperability, i.e., the ability to automatically interpret the shared information in a meaningful way, is one of the most important requirements for data analysis of different sources. In the area of clinical and epidemiological studies, the target of the National Research Data Infrastructure for Personal Health Data (NFDI4Health), interoperability of data collection instruments such as case report forms (CRFs), data dictionaries and questionnaires is critical. Retrospective integration of semantic codes into study metadata at item-level is important, as ongoing or completed studies contain valuable information, which should be preserved. We present a first version of a Metadata Annotation Workbench to support annotators in dealing with a variety of complex terminologies and ontologies. User-driven development with users from the fields of nutritional epidemiology and chronic diseases ensured that the service fulfills the basic requirements for a semantic metadata annotation software for these NFDI4Health use cases. The web application can be accessed using a web browser and the source code of the software is available with an open-source MIT license.

Root exudates are plant-derived, exported metabolites likely shaping root-associated microbiomes by acting as nutrients and signals. However, root exudation dynamics are unclear and thus also, if changes in exudation are reflected in changes in microbiome structure. Here, we assess commonalities and differences between exudates of different plant species, diurnal exudation dynamics, as well as the accompanying methodological aspects of exudate sampling. We find that exudates should be collected for hours rather than days as many metabolite abundances saturate over time. Plant growth in sterile, nonsterile, or sugar-supplemented environments significantly alters exudate profiles. A comparison of Arabidopsis thaliana, Brachypodium distachyon, and Medicago truncatula shoot, root, and root exudate metabolite profiles reveals clear differences between these species, but also a core metabolome for tissues and exudates. Exudate profiles also exhibit a diurnal signature. These findings add to the methodological and conceptual groundwork for future exudate studies to improve understanding of plant-microbe interactions., (© 2023. The Author(s).)

In six studies, we consistently observed costly third-party punishment (3PP) to decrease under ambiguity of the norm violation . Our research suggests that, under ambiguity, some people experience concerns about punishing unfairly. Those with higher (vs. lower) other-oriented justice sensitivity (Observer JS) reduced 3PP more pronouncedly (in Studies 1-3 and 4b, but not replicated in Studies 4-5). Moreover, those who decided to resolve the ambiguity (hence, removing the risk of punishing unfairly) exceeded the 3PP observed under no ambiguity (Study 4). However, we did not consistently observe these concerns about punishing unfairly to affect 3PP (Studies 4-5). We further considered whether people could use ambiguity as justification for remaining passive-thus, avoiding the costs of 3PP. We did not find conclusive evidence supporting this notion. Taken together, ambiguity entails a situational boundary of 3PP that sheds light on the prevalence of this behavior and, potentially, on its preceding decision-making.

In the extrusion of plastics, the thermal and material homogeneity of the plastic melt at the die entry are of high importance for the extrudate quality. While static mixers are widely used to improve the melt homogeneity, previous attempts at optimisation for reduced pressure loss and improved mixing had to be performed by hand and human experience, limiting the degrees of freedom and efficiency. A new automatic optimisation method based on the open source software OpenFOAM was developed. Using immersed boundary methods, new target functions in the pre-existing routine adjointShapeOptimizationFoam and an additional algorithm checking the suitability for additive manufacturing and fixing the geometry during run-time is presented. The new algorithm is used to optimise an existing static mixer based on an X-type geometry with integrated oil channels, maximising the heat exchange between oil and melt. Based on the results of these simulative optimisations, the best candidates were manufactured using selective laser melting and experimental trials were run. Experimental validation shows that with our optimisation algorithm, a pressure loss reduction of 10% could be achieved. The core melt temperature was reduced by 6 ∘C, improving the thermal homogenisation as well. While the main advantage of this method is the rapid optimisation taking the operating point into account, the trials also showed positive results in off-design operating points. This allows the low-cost design and manufacture of individualised static mixers.

Moral courage is manifested when bystanders intervene to stop or prevent others' norm transgressions, despite potential costs to themselves. Although theoretical models propose a key role of emotions, in particular anger, in the psychological processes underlying moral courage, to date this role is underexplored. In a behavioral study, we proposed a conceptual differentiation between experienced and expressed anger in response to a witnessed moral transgression. By staging the embezzlement of money from project funds in the lab, we tested whether anger arises in response to a witnessed moral norm transgression and scrutinized its unique contribution to predicting who intervenes and who remains inactive in the context of other theoretically relevant emotions (guilt, fear, and empathy). In addition, we investigated the role of bystanders' anger expression in response to the transgression. Lastly, we tested whether experienced and expressed anger reactions were predicted across time from dispositions. Our paradigm allowed us to obtain observational data of behavioral responses and anger expression and experienced emotion reports in response to a realistic moral norm transgression. Results showed that experienced anger increased after the transgression and uniquely predicted intervention. Experienced anger reaction was predictable across time from dispositional sensitivity to observed injustice. Anger expression was only loosely associated with anger experience and intervention, suggesting it may constitute a display of disapproval in itself. The present findings from a realistic moral transgression situation evidence the important role of anger in the psychological process underlying moral courage. (PsycInfo Database Record (c) 2022 APA, all rights reserved).

Polylactide acid (PLA) is one of the most used plastics in extrusion-based additive manufacturing (AM). Although it is bio-based and in theory biodegradable, its recyclability for fused filament fabrication (FFF) is limited due to material degradation. To better understand the material's recyclability, blends with different contents of recycled PLA (rPLA) are investigated alongside a coextruded filament comprised of a core layer with high rPLA content and a skin layer from virgin PLA. The goal was to determine whether this coextrusion approach is more efficient than blending rPLA with virgin PLA. Different filaments were extruded and subsequently used to manufacture samples using FFF. While the strength of the individual strands did not decrease significantly, layer adhesion decreased by up to 67%. The coextruded filament was found to be more brittle than its monoextruded counterparts. Additionally, no continuous weld line could be formed between the layers of coextruded material, leading to a decreased tensile strength. However, the coextruded filament proved to be able to save on master batch and colorants, as the outer layer of the filament has the most impact on the part's coloring. Therefore, switching to a coextruded filament could provide economical savings on master batch material.

Moral courage, that is, defending moral beliefs despite personal risks, is often seen as a hallmark of prosocial behavior. We argue that prosociality in moral courage is, however, complex. While its prosociality is often evident at a higher societal level, it can be contested in some aspects of morally courageous acts. We review the literature on two such aspects and highlight that differences and conflicts in moral beliefs, as well as the confrontational nature of many morally courageous acts, call into question prosociality. We recommend that future research takes the complexity of prosociality in moral courage into account to obtain a comprehensive and nuanced understanding of the psychological underpinnings of moral courage and its contributions to the functioning of societies., Competing Interests: Conflict of interest statement Nothing declared., (Copyright © 2021 Elsevier Ltd. All rights reserved.)