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514 results on '"Sauvanet, A."'

1. Toward a new paradigm of care: a surgical leaders’ Delphi consensus on the organizational factors of the new pancreas units (E-AHPBA PUECOF study)

Author

Cobianchi, Lorenzo, Dal Mas, Francesca, Abu Hilal, Mohammad, Adham, Mustapha, Alfieri, Sergio, Balzano, Gianpaolo, Barauskas, Giedrius, Bassi, Claudio, Besselink, Marc G., Bockhorn, Maximilian, Boggi, Ugo, Conlon, Kevin C., Coppola, Roberto, Dervenis, Christos, Dokmak, Safi, Falconi, Massimo, Fusai, Giuseppe Kito, Gumbs, Andrew A., Ivanecz, Arpad, Memeo, Riccardo, Radenković, Dejan, Ramia, Jose M., Rangelova, Elena, Salvia, Roberto, Sauvanet, Alain, Serrablo, Alejandro, Siriwardena, Ajith K., Stättner, Stefan, Strobel, Oliver, Zerbi, Alessandro, Malleo, Giuseppe, Butturini, Giovanni, and Frigerio, Isabella

Published
2024
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4. The Value of Textbook Outcome in Benchmarking Pancreatoduodenectomy for Nonfunctioning Pancreatic Neuroendocrine Tumors

Author

Partelli, Stefano, Fermi, Francesca, Fusai, Giuseppe K., Tamburrino, Domenico, Lykoudis, Panagis, Beghdadi, Nassiba, Dokmak, Safi, Wiese, Dominik, Landoni, Luca, Reich, Federico, Busch, O. R. C., Napoli, Niccolò, Jang, Jin-Young, Kwon, Wooil, Armstrong, Thomas, Allen, Peter J., He, Jin, Javed, Ammar, Sauvanet, Alain, Bartsch, Detlef K., Salvia, Roberto, van Dijkum, E. J. M. Nieveen, Besselink, Mark G., Boggi, Ugo, Kim, Sun-Whe, Wolfgang, Christofer L., and Falconi, Massimo

Published
2024
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6. The colibactin-producing Escherichia coli alters the tumor microenvironment to immunosuppressive lipid overload facilitating colorectal cancer progression and chemoresistance

Author

Nilmara de Oliveira Alves, Guillaume Dalmasso, Darja Nikitina, Amaury Vaysse, Richard Ruez, Lea Ledoux, Thierry Pedron, Emma Bergsten, Olivier Boulard, Lora Autier, Sofian Allam, Laurence Motreff, Pierre Sauvanet, Diane Letourneur, Pragya Kashyap, Johan Gagnière, Denis Pezet, Catherine Godfraind, Michel Salzet, Emmanuel Lemichez, Mathilde Bonnet, Imène Najjar, Christophe Malabat, Marc Monot, Denis Mestivier, Nicolas Barnich, Pankaj Yadav, Isabelle Fournier, Sean Kennedy, Amel Mettouchi, Richard Bonnet, Iradj Sobhani, and Mathias Chamaillard

Subjects
Colibactin-producing Escherichia coli, land’s cycle, lipid droplet, right-sided colorectal cancer, Diseases of the digestive system. Gastroenterology, RC799-869
Abstract

ABSTRACTIntratumoral bacteria flexibly contribute to cellular and molecular tumor heterogeneity for supporting cancer recurrence through poorly understood mechanisms. Using spatial metabolomic profiling technologies and 16SrRNA sequencing, we herein report that right-sided colorectal tumors are predominantly populated with Colibactin-producing Escherichia coli (CoPEC) that are locally establishing a high-glycerophospholipid microenvironment with lowered immunogenicity. It coincided with a reduced infiltration of CD8+ T lymphocytes that produce the cytotoxic cytokines IFN-γ where invading bacteria have been geolocated. Mechanistically, the accumulation of lipid droplets in infected cancer cells relied on the production of colibactin as a measure to limit genotoxic stress to some extent. Such heightened phosphatidylcholine remodeling by the enzyme of the Land’s cycle supplied CoPEC-infected cancer cells with sufficient energy for sustaining cell survival in response to chemotherapies. This accords with the lowered overall survival of colorectal patients at stage III-IV who were colonized by CoPEC when compared to patients at stage I-II. Accordingly, the sensitivity of CoPEC-infected cancer cells to chemotherapies was restored upon treatment with an acyl-CoA synthetase inhibitor. By contrast, such metabolic dysregulation leading to chemoresistance was not observed in human colon cancer cells that were infected with the mutant strain that did not produce colibactin (11G5∆ClbQ). This work revealed that CoPEC locally supports an energy trade-off lipid overload within tumors for lowering tumor immunogenicity. This may pave the way for improving chemoresistance and subsequently outcome of CRC patients who are colonized by CoPEC.

Published
2024
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7. Colibactin-producing Escherichia coli enhance resistance to chemotherapeutic drugs by promoting epithelial to mesenchymal transition and cancer stem cell emergence

Author

Guillaume Dalmasso, Antony Cougnoux, Tiphanie Faïs, Virginie Bonnin, Benoit Mottet-Auselo, Hang TT Nguyen, Pierre Sauvanet, Nicolas Barnich, Marine Jary, Denis Pezet, Julien Delmas, and Richard Bonnet

Subjects
Colorectal cancer, colibactin, pks, chemotherapy resistance, cancer stem cell, microbiota, Diseases of the digestive system. Gastroenterology, RC799-869
Abstract

ABSTRACTHuman colorectal cancers (CRCs) are readily colonized by colibactin-producing E. coli (CoPEC). CoPEC induces DNA double-strand breaks, DNA mutations, genomic instability, and cellular senescence. Infected cells produce a senescence-associated secretory phenotype (SASP), which is involved in the increase in tumorigenesis observed in CRC mouse models infected with CoPEC. This study investigated whether CoPEC, and the SASP derived from CoPEC-infected cells, impacted chemotherapeutic resistance. Human intestinal epithelial cells were infected with the CoPEC clinical 11G5 strain or with its isogenic mutant, which is unable to produce colibactin. Chemotherapeutic resistance was assessed in vitro and in a xenograft mouse model. Expressions of cancer stem cell (CSC) markers in infected cells were investigated. Data were validated using a CRC mouse model and human clinical samples. Both 11G5-infected cells, and uninfected cells incubated with the SASP produced by 11G5-infected cells exhibited an increased resistance to chemotherapeutic drugs in vitro and in vivo. This finding correlated with the induction of the epithelial to mesenchymal transition (EMT), which led to the emergence of cells exhibiting CSC features. They grew on ultra-low attachment plates, formed colonies in soft agar, and overexpressed several CSC markers (e.g. CD133, OCT-3/4, and NANOG). In agreement with these results, murine and human CRC biopsies colonized with CoPEC exhibited higher expression levels of OCT-3/4 and NANOG than biopsies devoid of CoPEC. Conclusion: CoPEC might aggravate CRCs by inducing the emergence of cancer stem cells that are highly resistant to chemotherapy.

Published
2024
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8. Optimizing Transmembrane Protein Assemblies in Nanodiscs for Structural Studies: A Comprehensive Manual

Author

Fernando Vilela, Cécile SAUVANET, Armel Bezault, Niels Volkmann, and Dorit Hanein

Subjects
Biology (General), QH301-705.5
Abstract

Membrane protein structures offer a more accurate basis for understanding their functional correlates when derived from full-length proteins in their native lipid environment. Producing such samples has been a primary challenge in the field. Here, we present robust, step-by-step biochemical and biophysical protocols for generating monodisperse assemblies of full-length transmembrane proteins within lipidic environments. These protocols are particularly tailored for cases where the size and molecular weight of the proteins align closely with those of the lipid islands (nanodiscs). While designed for single-span bitopic membrane proteins, these protocols can be easily extended to proteins with multiple transmembrane domains. The insights presented have broad implications across diverse fields, including biophysics, structural biology, and cryogenic electron microscopy (cryo-EM) studies.

Published
2024
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11. Tailoring the Use of Central Pancreatectomy Through Prediction Models for Major Morbidity and Postoperative Diabetes: International Retrospective Multicenter Study

Author

van Bodegraven, Eduard Antonie, Lof, Sanne, Jones, Leia, Aussilhou, Béatrice, Yong, Gao, Jishu, Wei, Klotz, Rosa, Rocha-Castellanos, Dario Missael, Matsumato, Ippei, de Ponthaud, Charles, Tanaka, Kimitaka, Biesel, Esther, Kauffmann, Emmanuele, Dumitrascu, Traian, Nagakawa, Yuichi, Martí-Cruchaga, Pablo, Roeyen, Geert, Zerbi, Alessandro, Goetz, Mara, de Meijer, Vincent E., Pessaux, Patrick, Ignatavicius, Povilas, Demir, Ihsan Ekin, Giuffrida, Mario, Tingstedt, Bobby, Marino, Marco Vito, Mastoridis, Sotiris, Brunner, Maximilian, Mora-Oliver, Isabel, Bortolato, Cecilia, Gulla, Aisté, Apers, Thomas, Hermand, Hélène, Mitsuka, Yusuke, Popescu, Irinel, Boggi, Ugo, Wittel, Uwe, Hirano, Satoshi, Gaujoux, Sébastien, Kamei, Keiko, Fernández-Del Castillo, Carlos, Hackert, Thilo, Kuirong, Jiang, Yi, Miao, Sauvanet, Alain, Besselink, Marc, Abu Hilal, Mohammad, and Dokmak, Safi

Published
2024
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12. ASO Visual Abstract: The Value of Textbook Outcome in Benchmarking Pancreatoduodenectomy for Nonfunctioning Pancreatic Neuroendocrine Tumors

Author

Partelli, Stefano, Fermi, Francesca, Fusai, Giuseppe K., Tamburrino, Domenico, Lykoudis, Panagis, Beghdadi, Nassiba, Dokmak, Safi, Wiese, Dominik, Landoni, Luca, Reich, Federico, Busch, O. R. C., Napoli, Niccolò, Jang, Jin-Young, Kwon, Wooil, Armstrong, Thomas, Allen, Peter J., He, Jin, Javed, Ammar, Sauvanet, Alain, Bartsch, Detlef K., Salvia, Roberto, van Dijkum, E. J. M. Nieveen, Besselink, M. G., Boggi, Ugo, Kim, Sun-Whe, Wolfgang, Christofer L., and Falconi, Massimo

Published
2024
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13. Somatostatin vs. Octreotide for Prevention of Postoperative Pancreatic Fistula The PREFIPS Randomized Clinical Trial A FRENCH 007 – ACHBT Study

Author

Gaujoux, Sébastien, Regimbeau, Jean-Marc, Piessen, Guillaume, Truant, Stéphanie, Foissac, Frantz, Barbier, Louise, Buc, Emmanuel, Adham, Mustapha, Fuks, David, Deguelte, Sophie, Muscari, Fabrice, Sulpice, Laurent, Vaillant, Jean-Christophe, Schwarz, Lilian, Sa Cunha, Antonio, Muzzolini, Milena, Dousset, Bertrand, Sauvanet, Alain, Abdoul, Hendy, Antomarchi, Olivier, Boudon, Marc, Carrere, Nicolas, Cauchy, François, Chebaro, Alexandre, Cherqui, Daniel, Crombe, Thibault, Cuellar, Emmanuel, D’avout, Ariane, de Ponthaud, Charles, Dubois, Clément, Durand-Zaleski, Isabelle, Dokmak, Safi, El Amrani, Mehdi, Gagniere, Johan, Gandon, Anne, Gayet, Brice, Hain, Elisabeth, Jeune, Florence, Kepenekian, Vahan, Leconte, Mahaut, Leroy, Bertrand, M’ba, Lena, Mare, Alexandru, Marques, Frédéric, Massault, Pierre-Philippe, Mensier, Alexandre, Millet, Guillaume, Navez, Julie, Perinel, Julie, Pelascani, Elise, Pezet, Denis, Piardi, Tullio, Pittau, Gabriella, Prodeau, Mathieu, Renard, Yohann, Rousseau, Géraldine, Schneck, Anne-Sophie, Slim, Karem, Suc, Bertrand, Tabchouri, Nicolas, Trechot, Boris, Tribillon, Ecoline, Tubbax, Candice, Tuech, Jean-Jacques, Tzedakis, Stylianos, Veziant, Julie, and Zerbib, Philippe

Published
2024
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14. Minimally invasive versus open distal pancreatectomy for resectable pancreatic neuroendocrine tumors: A propensity score matched multicentric comparative French study

Author

Addeo, Pietro, de Mathelin, Pierre, Doussot, Alexandre, Durin, Thibault, Canali, Giulia, Marchese, Ugo, Sauvanet, Alain, Dokmak, Safi, Cherkaoui, Zineb, Fuks, David, Laurent, Christophe, André, Marie, Ayav, Ahmet, Magallon, Cloe, Turrini, Olivier, Sulpice, Laurent, Robin, Fabien, Bachellier, Philippe, Souche, François-Régis, Bardol, Thomas, Perinel, Julie, Adham, Mustapha, Tzedakis, Stylianos, Birnbaum, David J., Facy, Olivier, Gagniere, Johan, Gaujoux, Sébastien, Tribillon, Ecoline, Roussel, Edouard, Schwarz, Lilian, Barbier, Louise, Regenet, Nicolas, Iannelli, Antonio, Regimbeau, Jean-Marc, Piessen, Guillaume, Truant, Stéphanie, and El Amrani, Mehdi

Published
2024
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15. The impact of cirrhosis on short and long postoperative outcomes after distal pancreatectomy

Author

Coinsin, Benjamin, Durin, Thibault, Marchese, Ugo, Sauvanet, Alain, Dokmak, Safi, Cherkaoui, Zineb, Fuks, David, Laurent, Christophe, Magallon, Cloe, Turrini, Olivier, Sulpice, Laurent, Robin, Fabien, Bachellier, Philippe, Addeo, Piettro, Birnbaum, David Jérémie, Roussel, Edouard, Schwarz, Lilian, Regimbeau, Jean-Marc, Piessen, Guillaume, Liddo, Guido, Girard, Edouard, Cailliau, Émeline, Truant, Stéphanie, and El Amrani, Mehdi

Published
2024
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18. Endoscopic papillectomy for ampullary lesions of minor papilla

Author

Vu Trung, Kien, Heise, Christian, Abou-Ali, Einas, Auriemma, Francesco, Karam, Elias, van der Wiel, Sophia E., Bruno, Marco J., Caillol, Fabrice, Giovannini, Marc, Masaryk, Viliam, Will, Uwe, Anderloni, Andrea, Pérez-Cuadrado-Robles, Enrique, Dugic, Ana, Meier, Benjamin, Paik, Woo H., Petrone, Maria C., Wichmann, Dörte, Dinis-Ribeiro, Mario, Gonçalves, Tiago C., Wedi, Edris, Schmidt, Arthur, Gulla, Aiste, Hoffmeister, Albrecht, Rosendahl, Jonas, Ratone, Jean Philippe, Saadeh, Rita, Repici, Alessandro, Deprez, Pierre, Sauvanet, Alain, Souche, Francois R., Fabre, Jean M., Muehldorfer, Steffen, Caca, Karel, Löhr, Matthias, Michl, Patrick, Krug, Sebastian, Regner, Sara, Gaujoux, Sebastien, and Hollenbach, Marcus

Published
2024
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21. Morphological control enables nanometer-scale dissection of cell-cell signaling complexes

Author

Dow, Liam P, Gaietta, Guido, Kaufman, Yair, Swift, Mark F, Lemos, Moara, Lane, Kerry, Hopcroft, Matthew, Bezault, Armel, Sauvanet, Cécile, Volkmann, Niels, Pruitt, Beth L, and Hanein, Dorit

Subjects
Biochemistry and Cell Biology, Biological Sciences, Nanotechnology, Bioengineering, Underpinning research, 1.1 Normal biological development and functioning, Generic health relevance, Image Processing, Computer-Assisted, Cryoelectron Microscopy, Proteins, Carbon, Signal Transduction
Abstract

Protein micropatterning enables robust control of cell positioning on electron-microscopy substrates for cryogenic electron tomography (cryo-ET). However, the combination of regulated cell boundaries and the underlying electron-microscopy substrate (EM-grids) provides a poorly understood microenvironment for cell biology. Because substrate stiffness and morphology affect cellular behavior, we devised protocols to characterize the nanometer-scale details of the protein micropatterns on EM-grids by combining cryo-ET, atomic force microscopy, and scanning electron microscopy. Measuring force displacement characteristics of holey carbon EM-grids, we found that their effective spring constant is similar to physiological values expected from skin tissues. Despite their apparent smoothness at light-microscopy resolution, spatial boundaries of the protein micropatterns are irregular at nanometer scale. Our protein micropatterning workflow provides the means to steer both positioning and morphology of cell doublets to determine nanometer details of punctate adherens junctions. Our workflow serves as the foundation for studying the fundamental structural changes governing cell-cell signaling.

Published
2022

23. Management and outcomes of hemorrhage after distal pancreatectomy: a multicenter study at high volume centers

Author

Turco, Célia, Dokmak, Safi, Cherkaoui, Zineb, Fuks, David, André, Marie, Ayav, Ahmet, Magallon, Cloé, Robin, Fabien, Bachellier, Philippe, Bardol, Thomas, Adham, Mustapha, Tzedakis, Stylianos, Lakkis, Zaher, Tribillon, Ecoline, Roussel, Edouard, Barbier, Louise, Piessen, Guillaume, Truant, Stéphanie, Duclos, Côme, Durin, Thibault, Marchese, Ugo, Sauvanet, Alain, Laurent, Christophe, Turrini, Olivier, Sulpice, Laurent, Addeo, Pietro, Souche, François R., Perinel, Julie, Birnbaum, David J., Facy, Olivier, Gagnière, Johan, Gaujoux, Sébastien, Schwarz, Lilian, Regenet, Nicolas, Iannelli, Antonio, Regimbeau, Jean M., Lenne, Xavier, El Amrani, Mehdi, Heyd, Bruno, and Doussot, Alexandre

Published
2024
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26. Pancreatoduodenectomy Following Preoperative Biliary Drainage Using Endoscopic Ultrasound-Guided Choledochoduodenostomy Versus a Transpapillary Stent: A Multicenter Comparative Cohort Study of the ACHBT–FRENCH–SFED Intergroup

Author

Janet, Julien, Albouys, Jeremie, Napoleon, Bertrand, Jacques, Jeremie, Mathonnet, Muriel, Magne, Julien, Fontaine, Marie, de Ponthaud, Charles, Durand Fontanier, Sylvaine, Bardet, Sylvia S. M., Bourdariat, Raphael, Sulpice, Laurent, Lesurtel, Mickael, Legros, Romain, Truant, Stephanie, Robin, Fabien, Prat, Frédéric, Palazzo, Maxime, Schwarz, Lilian, Buc, Emmanuel, Sauvanet, Alain, Gaujoux, Sebastien, and Taibi, Abdelkader

Published
2023
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27. The Brescia Internationally Validated European Guidelines on Minimally Invasive Pancreatic Surgery (EGUMIPS)

Author

Abu Hilal, Mohammad, van Ramshorst, Tess M.E., Boggi, Ugo, Dokmak, Safi, Edwin, Bjørn, Keck, Tobias, Khatkov, Igor, Ahmad, Jawad, Al Saati, Hani, Alseidi, Adnan, Azagra, Juan S., Björnsson, Bergthor, Can, Fatih M., D’Hondt, Mathieu, Efanov, Mikhail, Espin Alvarez, Francisco, Esposito, Alessandro, Ferrari, Giovanni, Groot Koerkamp, Bas, Gumbs, Andrew A., Hogg, Melissa E., Huscher, Cristiano G.S., Ielpo, Benedetto, Ivanecz, Arpad, Jang, Jin-Young, Liu, Rong, Luyer, Misha D.P., Menon, Krishna, Nakamura, Masafumi, Piardi, Tullio, Saint-Marc, Olivier, White, Steve, Yoon, Yoo-Seok, Zerbi, Alessandro, Bassi, Claudio, Berrevoet, Frederik, Chan, Carlos, Coimbra, Felipe J., Conlon, Kevin C.P., Cook, Andrew, Dervenis, Christos, Falconi, Massimo, Ferrari, Clarissa, Frigerio, Isabella, Fusai, Giuseppe K., De Oliveira, Michelle L., Pinna, Antonio D., Primrose, John N., Sauvanet, Alain, Serrablo, Alejandro, Smadi, Sameer, Badran, Ali, Baychorov, Magomet, Bannone, Elisa, van Bodegraven, Eduard A., Emmen, Anouk M.L.H., Giani, Alessandro, de Graaf, Nine, van Hilst, Jony, Jones, Leia R., Levi Sandri, Giovanni B., Pulvirenti, Alessandra, Ramera, Marco, Rashidian, Niki, Sahakyan, Mushegh A., Uijterwijk, Bas A., Zampedri, Pietro, Zwart, Maurice J.W., Alfieri, Sergio, Berti, Stefano, Butturini, Giovanni, Di Benedetto, Fabrizio, Ettorre, Giuseppe M., Giuliante, Felice, Jovine, Elio, Memeo, Riccardo, Portolani, Nazario, Ruzzenente, Andrea, Salvia, Roberto, Siriwardena, Ajith K., Besselink, Marc G., Asbun, Horacio J., Over, Paul, Saleh, Omar, and Rivizzigno, Piero

Published
2024
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37. Outcomes of rescue procedures in the management of locally recurrent ampullary tumors: A Pancreas 2000/EPC study

Author

Karam, Elias, Hollenbach, Marcus, Ali, Einas Abou, Auriemma, Francesco, Gulla, Aiste, Heise, Christian, Regner, Sara, Gaujoux, Sébastien, Regimbeau, Jean M., Kähler, Georg, Seyfried, Steffen, Vaillant, Jean C., De Ponthaud, Charles, Sauvanet, Alain, Birnbaum, David, Regenet, Nicolas, Truant, Stéphanie, Pérez-Cuadrado-Robles, Enrique, Bruzzi, Matthieu, Lupinacci, Renato M., Brunel, Martin, Belfiori, Giulio, Barbier, Louise, Salamé, Ephrem, Souche, Francois R., Schwarz, Lilian, Maggino, Laura, Salvia, Roberto, Gagniére, Johan, Del Chiaro, Marco, Leung, Galen, Hackert, Thilo, Kleemann, Tobias, Paik, Woo H., Caca, Karel, Dugic, Ana, Muehldorfer, Steffen, Schumacher, Brigitte, and Albers, David

Published
2023
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