90 results on '"Scarano F."'
Search Results
2. Physics-informed neural networks for dense reconstruction of vortex rings from particle tracking velocimetry.
- Author
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Steinfurth, B., Hassanein, A., Doan, N. A. K., and Scarano, F.
- Subjects
PARTICLE tracking velocimetry ,VELOCITY measurements ,VORTEX motion ,VELOCITY ,EQUATIONS - Abstract
Phase-resolved volumetric velocity measurements of a pulsed jet are conducted by means of three-dimensional particle tracking velocimetry (PTV). The resulting scattered and relatively sparse data are densely reconstructed by adopting physics-informed neural networks (PINNs), here regularized by the Navier–Stokes equations. It is shown that the assimilation remains robust even at low particle densities (ppp < 10 − 3 ) where the mean particle distance is larger than 10% of the outlet diameter. This is achieved by enforcing compliance with the governing equations, thereby leveraging the spatiotemporal evolution of the measured flow field. Thus, the PINN reconstructs unambiguously velocity, vorticity, and pressure fields, enabling a robust identification of vortex structures with a level of detail not attainable with conventional methods (binning) or more advanced data assimilation techniques (vortex-in-cell). The results of this article suggest that the PINN methodology is inherently suited to the assimilation of PTV data, in particular under conditions of severe data sparsity encountered in experiments with limited control of the seeding concentration and/or distribution. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
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3. The role of natural anti-oxidants in gut microbiota alteration subsequent to high fat diet (HFD)
- Author
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Mollace, V, primary, Mollace, R, additional, Macri, R, additional, and Scarano, F, additional
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- 2024
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4. The protective effect of bergamot polyphenolic fraction on reno-cardiac damage induced by DOCA-salt and unilateral renal artery ligation in rats
- Author
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Carresi, C, primary, Cardamone, A, additional, Coppoletta, A R, additional, Macri, R, additional, Lorenzo, F, additional, Scarano, F, additional, Mollace, R, additional, Ruga, S, additional, Nucera, S, additional, Musolino, V, additional, Gliozzi, M, additional, Palma, E, additional, Muscoli, C, additional, Volterrani, M, additional, and Mollace, V, additional
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- 2024
- Full Text
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5. On the scalability of helium-filled soap bubbles for volumetric PIV
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Grille Guerra, A. (author), Scarano, F. (author), Sciacchitano, A. (author), Grille Guerra, A. (author), Scarano, F. (author), and Sciacchitano, A. (author)
- Abstract
The scalability of experiments using PIV relies upon several parameters, namely illumination power, camera sensor and primarily the tracers light scattering capability. Given their larger cross section, helium-filled soap bubbles (HFSB) allow measurements in air flows over a significantly large domain compared to traditional oil or fog droplets. Controlling their diameter translates into scalability of the experiment. This work presents a technique to extend the control of HFSB diameter by geometrical variations of the generator. The latter expands the more limited range allowed by varying the relative helium-air mass flow rates. A theoretical model predicts the bubble size and production rate, which is verified experimentally by high-speed shadow visualization. The overall range of HFSB produced in a stable (bubbling) regime varies from 0.16 to 2.7 mm. Imaging by light scattering of such tracers is also investigated, in view of controversies in the literature on whether diffraction or geometrical imaging dominate the imaging regime. The light scattered by scaled HFSB tracers is imaged with a high-speed camera orthogonal to the illumination. Both the total energy collected on the sensor for a single tracer, as well as its peak intensity, are found to preserve scaling with the square of the diameter at object magnification of 10–1 or below, typical of PIV experiments. For large-scale volumetric applications, it is shown that varying the bubble diameter allows increasing both the measurement domain as well as the working distance of the imagers at 10 m and beyond. A scaling rule is proposed for the latter., Aerodynamics
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- 2024
6. Design of a Hydrogen-Powered Crashworthy eVTOL Using Multidisciplinary Analysis and Design Optimization
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Keijzer, D.M. (author), Simon Soria, C. (author), Arends, J.J. (author), Sarıgöl, B. (author), Scarano, F. (author), Castro, Saullo G.P. (author), Keijzer, D.M. (author), Simon Soria, C. (author), Arends, J.J. (author), Sarıgöl, B. (author), Scarano, F. (author), and Castro, Saullo G.P. (author)
- Abstract
As of present the Urban Air Mobility market has been dominated by fully electric aircraft. However, hydrogen vehicles have remained relatively undeveloped in this segment, also because hydrogen poses additional design complexities and uncertainties concerning crashworthiness, fuel cell cooling, and low volumetric density. Nevertheless, hydrogen might yield advantages in mission performance owing to its superior gravimetric energy density and greater sustainability when compared to batteries. In this paper, the design procedure of a four-passenger long-range hydrogen eVTOL using Multidisciplinary Analysis and Design Optimization (MADO) is presented. Using MADO, the mission energy of the eVTOL was minimized while abiding by the constraints rooting from the use of hydrogen. Based on this design, the conclusion can be made that the implementation of hydrogen eVTOLs in urban air mobility is feasible whilst taking into account constraints resulting from the use of hydrogen at the preliminary design stage. This led to an aircraft which excels at longer range due to the increased scalability of hydrogen fuel, but having a weight penalty due to auxiliary equipment which hampers its performance and results in a large fuselage and maximum takeoff weight., Aerodynamics, Group Giovani Pereira Castro
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- 2024
- Full Text
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7. Outlier detection for PIV statistics based on turbulence transport
- Author
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Saredi, E., Sciacchitano, A., and Scarano, F.
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- 2022
- Full Text
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8. Correction: Aerodynamic Interactions of Side-by-Side Rotors in Ground Proximity
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Dekker, H. N. J., primary, Ragni, D., additional, Baars, W. J., additional, Scarano, F., additional, and Tuinstra, M., additional
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- 2023
- Full Text
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9. Three-Dimensional Quantitative Flow Visualization Around a Thrust Reverser
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Hysa, I. (author), Tuinstra, Marthijn (author), Sciacchitano, A. (author), Scarano, F. (author), Schwartz, Nicholas (author), Harrison, Charles (author), Gebbink, Roy (author), Hysa, I. (author), Tuinstra, Marthijn (author), Sciacchitano, A. (author), Scarano, F. (author), Schwartz, Nicholas (author), Harrison, Charles (author), and Gebbink, Roy (author)
- Abstract
Volumetric particle tracking velocimetry measurements are performed in a low-speed wind tunnel to study the flow around a 1:12-scale aircraft model with jet engines operating with thrust reversers. The engine jet and freestream flow velocity are varied to yield a jet to freestream velocity ratio of Vjet/V∞ ranging from 1.5 to 6. Measurements at such scale (0.5 m3) require the use of strongly scattering helium-filled soap bubbles as flow tracers, which are introduced in both the jet and the freestream flow. The tracer’s three-dimensional motion is determined using an array of cameras and a Lagrangian particle tracking algorithm. The mean velocity field reveals the jet inner structure as well as its interaction with the freestream, the ground board, the nacelle, the fuselage, and the horizontal and vertical tails. The experiments allow detection of exhaust reingestion as well as the aerodynamic interference with control surfaces at the tail segments in a single measurement volume. The results are in good agreement with conventional temperature rake measurements while adding details of the flow topology and of the large-scale unsteady flow fluctuations. Finally, the jet reversal characteristics with varying freestreams and nozzle pressure ratios are assessed, demonstrating the feasibility and versatility of volumetric velocimetry measurements for industrial aerodynamics., Aerodynamics
- Published
- 2023
- Full Text
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10. Unsteady flow behaviour of multi-rotors in ground proximity
- Author
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Dekker, H.N.J. (author), Baars, W.J. (author), Scarano, F. (author), Tuinstra, Marthijn (author), Ragni, D. (author), Dekker, H.N.J. (author), Baars, W.J. (author), Scarano, F. (author), Tuinstra, Marthijn (author), and Ragni, D. (author)
- Abstract
The unsteady flow behaviour of two side-by-side rotors in ground proximity is experimentally investigated. The rotors induce a velocity distribution interacting with the ground causing the radial expansion of the rotor wakes. In between the rotors, an interaction of the two wakes takes place, resulting in an upward flow similar to a fountain. Two types of flow topologies are examined and correspond to two different stand-off heights between the rotors and the ground: the first one where the height of the fountain remains below the rotor disks, and a second one where it emerges above, being re-ingested. The fountain unsteadiness is shown to increase when re-ingestion takes place, determining a location switch from one rotor disk to the other, multiple times during acquisition. Consequently, variable inflow conditions are imposed on each of the two rotors. The fountain dynamics is observed at a frequency that is about two orders of magnitude lower than the blade passing frequency. The dominant characteristic time scale is linked to the flow recirculation path, relating this to system parameters of thrust and ground stand-off height. The flow field is analysed using proper orthogonal decomposition, in which coupled modes are identified. Results from the modal analysis are used to formulate a simple dynamic flow model of the re-ingestion switching cycle., Wind Energy, Aerodynamics
- Published
- 2023
- Full Text
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11. Aeropropulsive Performance Modelling of Over-The-Wing Propulsion at Incidence
- Author
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Dekker, H.N.J. (author), Tuinstra, M (author), Baars, W.J. (author), Scarano, F. (author), Ragni, D. (author), Dekker, H.N.J. (author), Tuinstra, M (author), Baars, W.J. (author), Scarano, F. (author), and Ragni, D. (author)
- Abstract
A semi-emperical model is developed, able to capture the aeropropulsive performance characteristics of Over-The-Wing propellers at incidence. The model is based on an hypothesis on the interactions of the propeller- and wing-induced flow fields. Effects of these interactions on the both the thrust and lift are written in a form in which the dominant design parameters appear explicitly. Both the flow hypothesis and model results are validated using experimental data of a single Over-The-Wing propeller. It is shown that for moderate angles of attack, the propulsive thrust is reduced by the wing’s circulation. For angles of attack greater than the stall angle of the isolated wing, thrust is increased by the ingestion of low momentum flow. The propeller is not able to delay stall but induces flow over the wing, which is returned as reduced pressure over the suction side. The model predictions closely match the experimental results for thrust, but integral loading measurements of the wing are required to validate the lift predictions., Aerodynamics, Wind Energy
- Published
- 2023
- Full Text
- View/download PDF
12. Correction: Aeropropulsive Performance Modelling of Over-The-Wing Propulsion at Incidence
- Author
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Dekker, H.N.J. (author), Tuinstra, Marthijn (author), Baars, W.J. (author), Scarano, F. (author), Ragni, D. (author), Dekker, H.N.J. (author), Tuinstra, Marthijn (author), Baars, W.J. (author), Scarano, F. (author), and Ragni, D. (author)
- Abstract
Wind Energy, Aerodynamics
- Published
- 2023
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13. Tire dependence for the aerodynamics of yawed bicycle wheels
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Jux, C. (author), Sciacchitano, A. (author), Scarano, F. (author), Jux, C. (author), Sciacchitano, A. (author), and Scarano, F. (author)
- Abstract
The aerodynamic characteristics of a modern road cycling wheel in cross wind are studied through force- and planar PIV measurements in the TU Delft Open Jet Facility. The performance of the 62 mm deep rim is evaluated for three tire profiles, and yaw angles up to 24°. All measurements are executed at 12.5 m/s (45 km/h) freestream- and wheel-rotational velocity. The wheel's rim-tire section in crosswind is found to behave similar to an airfoil at incidence, ultimately resulting in a reduction of the wheel's aerodynamic resistance with increasing yaw angle magnitude. This phenomenon, also referred to as the sail-effect, is limited by the stall angle of the tire-rim profile. The stall angle is found to depend critically on the tire's surface structure. Larger stall angles, resulting in lower resistance, are obtained if the tire profile triggers laminar-to-turbulent boundary layer transition., Flow Physics and Technology, Aerodynamics
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- 2023
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14. Italien und die Auflösung der Österreichisch-Ungarischen Monarchie
- Author
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Scarano F, SCARANO F., Institut für Zeitgeschichte Universität Hildesheim, and Scarano, F
- Subjects
Ersten Weltkrieg, Italien Österreich-Ungarn - Abstract
Quando scoppiò la prima guerra mondiale dei posti di responsabilità in Italia pensava ad una dissoluzione dell’Austria-Ungheria. Il ministro degli Esteri Sidney Sonnino voleva indebolirla ed ottenere la supremazia nel mare Adriatico e il confine sicuro al Brennero ma era contrario ad una sua dissoluzione. Ancora il 25 ottobre 1917 in Parlamento ribadì questa posizione. Tuttavia dopo la disfatta di Caporetto il Presidente del Consiglio Orlando decise di puntare anche a questa possibilità, come d’altra parte chiedevano importanti esponenti politici e della stampa, e di reclutare anche truppe tra i prigionieri di origine ceca, rumena e slava dell’esercito nemico. In realtà vi erano tre diverse politiche italiane riguardo all'Austria-Ungheria: quella di Sonnino con l'obbiettivo di una vittoria completa, ma contraria allo scioglimento dell'Austria-Ungheria, del Ministro del Tesoro Nitti favorevole ad una pace di compromesso con grandi rinunzie alle aspirazioni territoriali italiane e la politica di Orlando che nella sua propaganda di guerra s'impegnò per lo scioglimento dell'Austria-Ungheria e la creazione di nuovi Stati, ma in realtà si mantenne aperte tutte e tre le possibilità a seconda dell'andamento della guerra. Solo il 3 ottobre 1918 il governo italiano ultimi tra gli Alleati riconobbe il Comitato Nazionale Cecoslovacco come governo legittimo e combattente al suo fianco. Als der erste Weltkrieg ausbrach, dachte an verantwortlicher Stelle in Italien niemand an eine Zerschlagung Österreich-Ungarns Außenminister Sidney Sonnino wollte sie schwächen und die Vorherrschaft in der Adria und der sicheren Grenze am Brenner erlangen, war aber gegen ihre Auflösung. Nochmals am 25. Oktober 1917 im Parlament bekräftigte er diese Position. Nach der Niederlage von Karfreit beschloss Ministerpräsident Orlando jedoch, auch diese Möglichkeit anzustreben, wie es wichtige Politiker und die Presse forderten; er befürwortete die Rekrutierung von Truppen unter den Gefangenen tschechischer, rumänischer und slawischer Herkunft. Tatsächlich hatten die Italiener gegenüber Österreich-Ungarn drei mögliche Arten des Vorgehens zu erwägen gehabt: die Politik von Sonnino mit dem Ziel eines umfassenden Sieges, aber gegen eine Auflösung von Österreich-Ungarn; diejenige von Schatzminister Nitti für einen Kompromißfrieden mit großen Verzichtsleistungen Italiens, und die Politik Orlandos, der sich zwar in seiner Kriegspropaganda für die Auflösung Österreich-Ungarns und die Schaffung der neuen Staaten einsetzte, aber in Wirklichkeit je nach dem Ausgang des Krieges sich alle diese drei Möglichkeiten offenhielt. Erst am 3. Oktober 1918 erkannte die italienische Regierung als letzte der Alliierten das tschechoslowakische Nationlkomitee als legitime Regierung und als kriegführend an ihrer Seite an
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- 2022
15. L'Italia e il problema sudtirolese da De Gasperi a Moro
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Scarano F, SCARANO F., A. Di Michele, A. Gottsmann, L. Monzali, K. Ruzicic-Kessler, and Scarano, F
- Subjects
Italia e Sudtirolo 1945-1969, Alcide De Gasperi, Aldo Moro, Rapporti tra Austria e Italia, terrorismo sudtirolese - Abstract
Il problema sudtirolese nei suoi molteplici aspetti politici e diplomatici dal 1946 al 1969. Dal tentativo dei sudtirolesi di ottenere l’autodeterminazione nel 1945, agli accordi De Gasperi-Gruber, fino alla soluzione della questione nel 1969 grazie alla politica di Aldo Moro. Viene affrontato anche il drammatico periodo del terrorismo sudtirolese, l’azione del governo italiano per contrastarlo, le difficili trattative con l’Austria e il ruolo di altri importanti attori italiani, austrici e sudtirolesi come Antonio Segni, Amintore Fanfani, Mario Scelba, Bruno Kreisky, Silvius Magnago
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- 2022
16. Aeropropulsive Performance Modelling of Over-The-Wing Propulsion at Incidence
- Author
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Dekker, H.N.J., Tuinstra, M, Baars, W.J., Scarano, F., and Ragni, D.
- Abstract
A semi-emperical model is developed, able to capture the aeropropulsive performance characteristics of Over-The-Wing propellers at incidence. The model is based on an hypothesis on the interactions of the propeller- and wing-induced flow fields. Effects of these interactions on the both the thrust and lift are written in a form in which the dominant design parameters appear explicitly. Both the flow hypothesis and model results are validated using experimental data of a single Over-The-Wing propeller. It is shown that for moderate angles of attack, the propulsive thrust is reduced by the wing’s circulation. For angles of attack greater than the stall angle of the isolated wing, thrust is increased by the ingestion of low momentum flow. The propeller is not able to delay stall but induces flow over the wing, which is returned as reduced pressure over the suction side. The model predictions closely match the experimental results for thrust, but integral loading measurements of the wing are required to validate the lift predictions.
- Published
- 2023
17. PIV Analysis Of Skin Friction And Coherent Structures In Turbulent Drag Reduction Regimes
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Scarano, F., primary, Kempaiah, K.U., additional, and Kotsonis, M., additional
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- 2022
- Full Text
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18. On the wake dynamics of a cylinder with flexible splitter plate
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Saiz, G.G., primary, Sciacchitano, A., additional, and Scarano, F., additional
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- 2022
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19. A Novel Multi-Step Multi-Exposure PTV Algorithm With Adaptive Time Separation
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Saredi, E., primary, Sciacchitano, A., additional, and Scarano, F., additional
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- 2022
- Full Text
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20. Incommensurable time intervals for multi-exposure PTV
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Hysa, I., primary, Scarano, F., additional, Sciacchitano, A., additional, and Tuinstra, M., additional
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- 2022
- Full Text
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21. Thrust-Reverser Investigation by Large-Scale 3D PIV
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Hysa, I. (author), Tuinstra, Marthijn (author), Lammers, K (author), Scarano, F. (author), Sciacchitano, A. (author), Gebbink, Roy (author), Harrison, C (author), Hysa, I. (author), Tuinstra, Marthijn (author), Lammers, K (author), Scarano, F. (author), Sciacchitano, A. (author), Gebbink, Roy (author), and Harrison, C (author)
- Abstract
Volumetric PIV measurements are performed to study the flow development around a 1:12 scale model of a thrust reverser in a low-speed wind tunnel. The thrust-reverser operates in a freestream flow of 3-5 m/s and with a jet to freestream velocity ratio Vjet/Vinf ranging from 1.5 to 6. Making use of sub-millimeter helium-filled soap bubbles, large-scale PIV measurements are performed that encompass a 3D domain of approximately 0.4 m3. The flow exiting the thrust-reverser features two inclined jets that interact with the wind tunnel free stream, the nacelle, fuselage and ultimately the tunnel walls. Such interactions result in highly three-dimensional patterns and jets large scale fluctuations. The jet reversal characteristics with varying freestream velocity and nozzle pressure ratio are characterized quantitatively. The work demonstrates the feasibility of quantitative inspection of the flow behavior in a three dimensional domain for industrial applications., Green Open Access added to TU Delft Institutional Repository ‘You share, we take care!’ – Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public., Aerodynamics
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- 2022
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22. A physics-based description and modelling of the wall-pressure fluctuations on a serrated trailing edge
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Lima Pereira, L.T. (author), Avallone, F. (author), Ragni, D. (author), Scarano, F. (author), Lima Pereira, L.T. (author), Avallone, F. (author), Ragni, D. (author), and Scarano, F. (author)
- Abstract
A physical description of the flow mechanisms that govern the distribution of the wall-pressure fluctuations over the surface of a serrated trailing edge is proposed. Three main mechanisms that define the variation of turbulent pressure fluctuations across the serrated edge are discussed and semi-empirical models are formulated accordingly. It is shown that the intensity of the wall-pressure fluctuations increases at the tips under the effect of an increased convective velocity as a result of sidewise momentum diffusion. Furthermore, the change of impedance across the edge causes a local reduction of the pressure fluctuations in the vicinity of the trailing edge. Finally, aerodynamic loading over the serrations due to the non-symmetric flow created at different angles of attack establishes secondary flow patterns that induce higher wall-pressure fluctuations over the serration edges. The latter effect is present only for serrations under high aerodynamic loading, while the former ones are observed under any conditions. Semi-empirical models are formulated for predicting the variation of the wall-pressure fluctuations over the serration surface based on the three physical mechanisms described. These models are calibrated and compared against experiments conducted on a symmetric airfoil model at high Reynolds numbers., Wind Energy, Aerodynamics
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- 2022
- Full Text
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23. Outlier detection for PIV statistics based on turbulence transport
- Author
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Saredi, E. (author), Sciacchitano, A. (author), Scarano, F. (author), Saredi, E. (author), Sciacchitano, A. (author), and Scarano, F. (author)
- Abstract
The occurrence of data outliers in PIV measurements remains nowadays a problematic issue; their effective detection is relevant to the reliability of PIV experiments. This study proposes a novel approach to outliers detection from time-averaged three-dimensional PIV data. The principle is based on the agreement of the measured data to the turbulent kinetic energy (TKE) transport equation. The ratio between the local advection and production terms of the TKE along the streamline determines the admissibility of the inquired datapoint. Planar and 3D PIV experimental datasets are used to demonstrate that in the presence of outliers, the turbulent transport (TT) criterion yields a large separation between correct and erroneous vectors. The comparison between the TT criterion and the state-of-the-art universal outlier detection from Westerweel and Scarano (Exp Fluids 39:1096–1100, 2005) shows that the proposed criterion yields a larger percentage of detected outliers along with a lower fraction of false positives for a wider range of possible values chosen for the threshold. Graphical abstract: [Figure not available: see fulltext.], Aerodynamics
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- 2022
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24. Wake scaling of actuator discs in different aspect ratios
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Huang, M. (author), Ferreira, Carlos (author), Sciacchitano, A. (author), Scarano, F. (author), Huang, M. (author), Ferreira, Carlos (author), Sciacchitano, A. (author), and Scarano, F. (author)
- Abstract
The wake recovery from planar porous actuators that surrogate the effect of wind turbines is investigated, focusing on rectangular shapes for vertical axis wind turbines (VAWTs). We proposed an effective mixing diameter D∗ to scale the streamwise momentum recovery for actuators of arbitrary shape. The length-scale D∗ is given by the ratio between frontal area and disc perimeter characterising the wake-freestream interface, whereby the momentum loss and the turbulent exchange of momentum take place. Wind tunnel experiments of planar actuators from porous plates are presented. The three-dimensional development of the wake is surveyed up to six widths/diameters downstream of the actuators making use of robotic particle image velocimetry with helium-filled soap bubbles as flow tracers. The recovery rate analysis is performed using D∗ for wake normalisation. The scaled wake data agrees well among actuators in different shapes. And it is significantly improved for rectangular actuators, comparing with existing scaling lengths. The flow behaviour is confirmed with numerical simulations of VAWT wakes with different aspect ratios, indicating the validity of this scaling concept for wind turbine wake modelling., Wind Energy, Aerodynamics
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- 2022
- Full Text
- View/download PDF
25. Aerodynamic Interactions of Side-by-Side Rotors in Ground Proximity
- Author
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Dekker, H.N.J. (author), Ragni, D. (author), Baars, W.J. (author), Scarano, F. (author), Tuinstra, Marthijn (author), Dekker, H.N.J. (author), Ragni, D. (author), Baars, W.J. (author), Scarano, F. (author), and Tuinstra, Marthijn (author)
- Abstract
An experimental investigation is conducted to study the aerodynamic behavior of a two-rotor system in ground proximity. The counter-rotating rotors are placed side-by-side in the hovering condition. The time-averaged and unsteady flow behavior is studied when the rotor-to-rotor lateral distance and the distance between the rotors and the ground are varied. The experiments are performed using three-dimensional large-scale volumetric velocimetry with helium-filled soap bubbles as tracers, tracked by the particle motion analysis technique “Shake-The-Box.” The mean velocity field reveals the wake deflection due to the ground plane and the formation of toroidal-shape regions of separated flow below each rotor. The interaction of the wall jets formed by slipstream deflection results in a separation line with the flow emerging from the wall in a fountain-like pattern. Regimes of flow re-ingestion occur when the rotors are sufficiently far apart. The flowfield exhibits the tendency toward asymmetric states, during which the fountain flow column and the domain of re-ingestion shift closer to one of the rotors. A generic classification of flow regimes is proposed in relation to the behavior of two rotors in ground effect., Wind Energy, Aerodynamics
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- 2022
- Full Text
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26. Dense velocity reconstruction with VIC-based time-segment assimilation
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Scarano, F. (author), Schneiders, J.F.G. (author), Gonzalez Saiz, G. (author), Sciacchitano, A. (author), Scarano, F. (author), Schneiders, J.F.G. (author), Gonzalez Saiz, G. (author), and Sciacchitano, A. (author)
- Abstract
The vortex-in-cell time-segment assimilation (VIC-TSA) method is introduced. A particle track is obtained from a finite number of successive time samples of the tracer’s position and velocity can be used for reconstruction on a Cartesian grid. Similar to the VIC + technique, the method makes use of the vortex-in-cell paradigm to produce estimates of the flow state at locations and times other than the measured ones. The working principle requires time-resolved measurements of the particles’ velocity during a finite time interval. The work investigates the effects of the assimilated length on the spatial resolution of the velocity field reconstruction. The working hypotheses of the VIC-TSA method are presented here along with the numerical algorithm for its application to particle tracks datasets. The novel parameter governing the reconstruction is the length of the time-segment chosen for the data assimilation. Three regimes of operation are identified, based on the track length and the geometrical distance between neighbouring tracks. The regime of adjacent tracks arguably provides the optimal trade-off between spatial resolution and computational effort. The VIC-TSA spatial resolution is evaluated first by a numerical exercise; a 3D sine wave lattice is reconstructed at different values of the particles concentration. The modulation appears to reduce (cut-off delay) when the time-segment length is increased. Large-scale PIV experiments in the wake of a circular cylinder at Red = 27,000 are used to evaluate the method’s suitability to real data, including noise and data outliers. Both primary vortex structures in the Kármán wake as well as interconnecting ribs are present in this complex flow field, with a typical diameter close to the average inter-particle distance. When the time-segment is increased to adjacent tracks and beyond, a more regular time dependence of local and Lagrangian properties is observed, confirming the suitability of the time-se, Aerodynamics
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- 2022
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27. Experimental analysis of the effect of dynamic induction control on a wind turbine wake
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van der Hoek, D.C. (author), Frederik, J.A. (author), Huang, M. (author), Scarano, F. (author), Ferreira, Carlos (author), van Wingerden, J.W. (author), van der Hoek, D.C. (author), Frederik, J.A. (author), Huang, M. (author), Scarano, F. (author), Ferreira, Carlos (author), and van Wingerden, J.W. (author)
- Abstract
Dynamic induction control (DIC) has proven to be an effective method of increasing the power output for a wind farm in both simulation studies and wind tunnel experiments. By pitching the blades of a wind turbine periodically, the recovery of the low-velocity wake is accelerated, thereby increasing the energy available to downstream turbines. The wake itself of a turbine operating with DIC has not yet been studied experimentally. This paper presents a wind tunnel experiment where the wake of a wind turbine under periodic excitation is investigated. Using three-dimensional particle image velocimetry, the velocity field behind the turbine was reconstructed. Analysis of the velocity fields indicated that the available power in the wake increases when using DIC. This increase was partially due to a lower average thrust force experienced by the turbine with DIC. However, a large difference was seen between measurement results and actuator disk theory, indicating enhanced recovery of the wake is contributing to the increased energy. Instantaneous measurements visualizing the development of blade tip vortices also showed how the location of vortex breakdown, which is directly related to re-energizing the wake, shifts over time with DIC. We believe this shifting location is contributing to the enhanced wake recovery of DIC, providing more energy to downstream wind turbines., Team Jan-Willem van Wingerden, Wind Energy, Aerodynamics
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- 2022
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28. PIV analysis of skin friction and coherent structures in turbulent drag reduction regimes
- Author
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Scarano, F. (author), Ujjaini Kempaiah, K. (author), Kotsonis, M. (author), Scarano, F. (author), Ujjaini Kempaiah, K. (author), and Kotsonis, M. (author)
- Abstract
In the present work, planar and tomographic PIV are used to investigate how the organisation of wall turbulence is altered when actuators are operated with the objective of reducing the skin friction drag. When the wall is mechanically oscillated in the spanwise direction, high-resolution planar PIV enable the direct measurement of wall shear and a 15% reduction of skin friction is observed. The use of tomographic PIV enables access to the three dimensional organisation of low- and high-speed streaks, along with ejection events and associated hairpin vortices. These observations help forming a conceptual model of the salient drag reduction mechanism, whereby hairpin auto-generation is inhibited through a tilting action at the tail of low-speed streaks. The second part of the study documents an effort to surrogate the mechanical oscillation by means of a densely distributed array of AC-DBD plasma actuators. The latter are first characterised in quiescent flow, where the induced velocity distribution is obtained and compared to the solution of the classical second Stokes problem that models the mechanical oscillation. The induced peak of spanwise velocity is found to surpass the velocity of the oscillating wall. However, the wall jet height develops further away from the wall. More importantly, the spatially inhomogeneous distribution of the unsteady body force produces an unwanted lattice of starting streamwise vortices. The latter are deemed to be detrimental for the purpose of drag reduction, compared to the orderly and homogeneous sideways motion induced by mechanical wall motion. The application of the AC-DBD actuator currently leads to a pronounced momentum deficit in the logarithmic region and increases skin friction. Finally, further research directions are anticipated, that potentially circumvent the formation of streamwise vortices by means of AC-DBD actuators operating in steady regime., Aerodynamics
- Published
- 2022
29. A review of experiments on stationary bluff body wakes
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Forouzi Feshalami, Behzad (author), He, Shuisheng (author), Scarano, F. (author), Gan, Lian (author), Morton, Chris (author), Forouzi Feshalami, Behzad (author), He, Shuisheng (author), Scarano, F. (author), Gan, Lian (author), and Morton, Chris (author)
- Abstract
Experimental studies dealing with the wake of isolated stationary bluff bodies are reviewed. After briefly recalling the pioneering works in this domain, the paper focuses on recent research conducted with the latest experimental methods and techniques. The review encompasses a range of topics, including the effects of bluff body geometry (non-circular cross sections and non-uniformity in spanwise direction), steady and unsteady (periodic and non-periodic) inflow conditions, surface proximity (rigid wall, confinement, and water free surface) and non-Newtonian fluids. Focus is brought to the flow physics of the wakes, especially the complex three-dimensional and oscillatory behaviors induced by the periodic vortex shedding phenomenon. The paper aims to offer a critical and systematic review of new knowledge and findings on the subject area, as well as the most frequently adopted experimental techniques. The review also helps identifying knowledge gaps in the literature that need to be addressed in future investigations., Green Open Access added to TU Delft Institutional Repository 'You share, we take care!' - Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public., Aerodynamics
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- 2022
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30. Feature-based analysis of a turbulent boundary layer under spanwise wall oscillation
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Ujjaini Kempaiah, K. (author), Scarano, F. (author), Ujjaini Kempaiah, K. (author), and Scarano, F. (author)
- Abstract
Spanwise wall oscillations alter the organization of low-speed streaks and ejections in turbulent boundary layers, eventually leading to skin friction drag reduction. Such flow regimes are represented by pointwise statistics or spatial correlation. This work attempts to quantify the systematic distortions of the dominant turbulent structures by feature-analysis, intended to overcome the dispersion observed in pointwise statistics and correlation functions. Furthermore, data from tomographic particle image velocimetry are employed to clarify the mechanism that inhibits hairpin auto-generation, as described in Kempaiah et al. ["3-dimensional particle image velocimetry based evaluation of turbulent skin-friction reduction by spanwise wall oscillation,"Phys. Fluids 32(8), 085111 (2020)]. Based on the instantaneous distribution of Reynolds stresses, a specific spatial template is defined for low-speed streaks and flow ejections. Events corresponding to this template are collected and parametrized with their occurrence, geometrical properties (length and orientation), and dynamics (intensity). The approach is compared with most practiced statistical analysis to explain the significance of the features extracted by the detection algorithm in relation to the drag reduction mechanism. Data comparing stationary and oscillating wall in a drag-reducing regime (A+osc = 100, T+osc = 100) are investigated in the near-wall region (y+ < 100). Ejections and low-speed streaks systematically exhibit a positive pitch, supporting the hypothesis that only the rear region, close to the wall, is affected by the wall motion. A side-tilt of elongated ejection events is observed past the phase of maximum oscillation velocity, which is hypothesized to inhibit hairpin auto-generation. The latter indicates a phase dependence of the side-tilt in the oscillating regime. The results also indicate that low-speed streaks and ejection events are reduced by approximately 10% and 15%, respectively, Aerodynamics
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- 2022
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31. Aeroacoustics of sawtooth trailing-edge serrations under aerodynamic loading
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Lima Pereira, L.T. (author), Ragni, D. (author), Avallone, F. (author), Scarano, F. (author), Lima Pereira, L.T. (author), Ragni, D. (author), Avallone, F. (author), and Scarano, F. (author)
- Abstract
The impact of aerodynamic loading on a serrated trailing edge is studied experimentally. Aerodynamic and acoustic measurements are conducted on a sawtooth-shaped trailing edge, retrofitted to a flat plate featuring a trailing-edge flap, and placed at incidence to the free-stream flow. The turbulent flow across the trailing edge is inspected by time-resolved three-dimensional velocity field measurements obtained from 4D-PIV, while the wall-pressure fluctuations are measured with surface-embedded microphones. Results discuss the relation between the velocity fluctuations over the serrations, the surface pressure fluctuations, and the far-field noise spectra. The aerodynamic analysis discusses the effect of counter-rotating vortex pairs, generated by the pressure imbalance across the edges of the serrations under loading. It is shown that the interaction of these vortices with the incoming turbulence affects the intensity of the wall-pressure spectrum at the outer rim of the serration surface. On the suction side, the intensity of the pressure fluctuations from the incoming boundary layer dominates over that induced by the vortex pairs. On the pressure side, instead, the velocity gradient prescribed by the vortex pairs produces a significant increase of the pressure fluctuations around the edges. The resulting spatial distribution of the wall-pressure fluctuations directly affects the far-field noise. Scattering predictions carried out with the wall-pressure fluctuations in the centre and root (on the suction side) exhibit good agreement with the measured noise in the low-frequency range, whereas using the surface pressure data at the tip of the serration (on the pressure side) yields a better prediction in the high-frequency range., Wind Energy, Aerospace Engineering, Aerodynamics
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- 2022
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32. On the closure of Collar’s triangle by optical diagnostics
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Gonzalez Saiz, G. (author), Sciacchitano, A. (author), Scarano, F. (author), Gonzalez Saiz, G. (author), Sciacchitano, A. (author), and Scarano, F. (author)
- Abstract
An experimental methodology is proposed to study aeroelastic systems with optical diagnostics. The approach locally evaluates the three physical mechanisms that produce the forces involved in Collar’s triangle, namely aerodynamic, elastic, and inertial forces. Flow and object surface tracers are tracked by a volumetric particle image velocimetry (PIV) system based on four high-speed cameras and LED illumination. The images are analysed with Lagrangian particle tracking techniques, and the flow tracers and surface markers are separated based on the different properties of their images. The inertial and elastic forces are obtained solely analysing the motion and the deformation of the solid object, whereas the aerodynamic force distribution is obtained with pressure from PIV techniques. Experiments are conducted on a benchmark problem of fluid–structure interaction, featuring a flexible panel installed at the trailing edge of a cylinder. Data are collected in the resonant regime, where the panel exhibits a two-dimensional motion. The estimation of inertial and elastic forces is obtained enforcing a high-order polynomial fit to the surface motion and deformation. The aerodynamic loads on the panel are challenged by the need to devise adaptive boundary conditions complying with the panel motion. The closure of Collar’s triangle yields overall residuals of about one-half of the inertial force taken as reference. The simultaneous measurement of the three forces paves the way to assessing the equilibrium of forces closing the Collar’s triangle. The latter can be intended for uncertainty evaluation or, when only two forces are measured, for estimation of the remaining Collar element. Graphical Abstract: [Figure not available: see fulltext.]., Aerodynamics
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- 2022
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33. TU Delft COVID-app: A tool to democratize CFD simulations for SARS-CoV-2 infection risk analysis
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Engler Faleiros, D. (author), van den Bos, W. (author), Botto, L. (author), Scarano, F. (author), Engler Faleiros, D. (author), van den Bos, W. (author), Botto, L. (author), and Scarano, F. (author)
- Abstract
This work describes a modelling approach to SARS-CoV-2 dispersion based on experiments. The main goal is the development of an application integrated in Ansys Fluent to enable computational fluid dynamics (CFD) users to set up, in a relatively short time, complex simulations of virion-laden droplet dispersion for calculating the probability of SARS-CoV-2 infection in real life scenarios. The software application, referred to as TU Delft COVID-app, includes the modelling of human expiratory activities, unsteady and turbulent convection, droplet evaporation and thermal coupling. Data describing human expiratory activities have been obtained from selected studies involving measurements of the expelled droplets and the air flow during coughing, sneezing and breathing. Particle Image Velocimetry (PIV) measurements of the transient air flow expelled by a person while reciting a speech have been conducted with and without a surgical mask. The instantaneous velocity fields from PIV are used to determine the velocity flow rates used in the numerical simulations, while the average velocity fields are used for validation. Furthermore, the effect of surgical masks and N95 respirators on particle filtration and the probability of SARS-CoV-2 infection from a dose-response model have also been implemented in the application. Finally, the work includes a case-study of SARS-CoV-2 infection risk analysis during a conversation across a dining/meeting table that demonstrates the capability of the newly developed application., Transport Engineering and Logistics, Complex Fluid Processing, Aerodynamics
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- 2022
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34. Outlier detection for PIV statistics based on turbulence transport
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Saredi, E., primary, Sciacchitano, A., additional, and Scarano, F., additional
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- 2021
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35. Influence of a forward-facing step on crossflow instability and transition
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Rius Vidales, A.F., Kotsonis, M., Scarano, F., and Delft University of Technology
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Crossflow Instability ,Surface Irregularities ,Forward-Facing Step ,Swept wings ,Boundary-layer transition - Abstract
The market growth expected for commercial aviation in the coming decades and the increasing social awareness regarding the effects of global warming are driving significant technological developments necessary for emission reduction in future transport aircraft. From the aerodynamics perspective, a significant increase in aircraft efficiency can be obtained by applying Laminar Flow Control (LFC) techniques. The objective of LFC techniques is to reduce the skin-friction drag component by delaying the laminar-turbulent transition through the stabilisation of boundary-layer instabilities. Relevant to high-subsonic transport aircraft is the development of Crossflow (CF) instability, which manifests as a series of co-rotating vortices inside the boundarylayer flow on swept aerodynamic surfaces...
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- 2022
36. Development of robotic volumetric PIV: with applications in sports aerodynamics
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Jux, C., Scarano, F., Sciacchitano, A., and Delft University of Technology
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Quantitative flow visualization ,Coaxial volumetric velocimetry ,Pressure measurements ,Sport aerodynamics ,Particle Image Velocimetry (PIV) ,Low speed aerodynamics ,Robotic PIV - Abstract
Particle image velocimetry (PIV) is the state of the art for quantitative, full-field, 3D flow diagnostics. Despite the maturity of the technique, two bottlenecks are identified which are addressed in this thesis: the achievable measurement volume size, and the optical access to geometrically complex objects. Both aspects are well illustrated when considering the human body in sports action. Characterising the aerodynamic flow topology around an athlete demands measurement volumes on the cubic-meter scale, whereas the simultaneous illumination and imaging of the flow near the athlete’s body is challenged by the geometric complexity of the human body and the sports equipment. Focusing on sport performance, especially in timed disciplines, it is recognized that due to the shape of the human body, the aerodynamic resistance is often dominated by pressure drag. Therefore, a third element addressed in this thesis is the PIV-based pressure evaluation in the flow and on an object surface. To overcome the identified measurement constraints, a PIV system for the 3D diagnostics of large-scale and low-speed flows has been developed, synthesizing advancements in PIV imaging and illumination hardware, automation technology, tracer particle generation, and particle tracking algorithms. The so called robotic volumetric PIV concept is proposed in Part I of this thesis, along with dedicated data analysis methods to retrieve the shape of the test object, the total pressure in the fluid flow, and the aerodynamic pressure on the object surface. Part II features applications of the proposed tools in the context of sport aerodynamics, with specific examples in cycling and swimming.
- Published
- 2022
37. Ring of fire as a novel approach to study cycling aerodynamics
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Spoelstra, A.M.C.M.G., Scarano, F., Sciacchitano, A., and Delft University of Technology
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PIV ,Sports aerodynamics ,Helium filled soap bubbles ,On-site drag evaluation ,Cycling aerodynamics - Abstract
The research presented in this thesis introduces a new measurement concept for on-site aerodynamic measurements based on large-scale stereoscopic particle image velocimetry (stereo-PIV) measurements past an athlete, a vehicle or an object travelling through a quiescent environment. The analysis of the momentumdeficit past the transit poses the basis to estimate the aerodynamic drag. For such an approach, where the object crosses the illuminated measurement plane, the experimental method is referred with the name“Ring of Fire” (RoF). The first part of this work presents the development and assessment of the Ring of Fire concept through the study of cycling aerodynamics. A feasibility study is performed in which two RoF experimentswith a cyclist are conducted, indoor and outdoor,mimicking respectively track and road cycling. During these experiments attention is placed on the effects of the environmental conditions and the confinement of the measurement region. Furthermore, the experiments cover different postures of the cyclist (time trial and upright) with the aimto directly measure the effect of posture on aerodynamic drag and its detectability with the RoF. Despite differences between the two experiments in the cyclist geometry, bike geometry, and the cycling speed, the flow fields in the near wake of the riders compare well between both experiments and to literature. In terms of drag estimation, a clear distinction in upright vs. time trial drag area is found for both experiments, with the upright posture yielding higher drag area by about 20-35% with respect to the time trial posture. The comparison of these drag values with literature data, however, could not yield a conclusive assessment, given the large dispersion (approx. 50%) of the literature data due to many varying parameters, like rider posture, bikes geometries and testing conditions. Furthermore, the uncertainty of the measured drag and its dependency upon experimental conditions and the image processing parameters have not yet been addressed. Knowledge of the minimum detectable drag variation is relevant when measurements are intended to perform aerodynamic optimisation, therefore, a sensitivity analysis is conducted that assesses how the estimated drag is affected by the choice of PIV image processing parameters. The size of the cross-section considered in the control volume formulation is also investigated. It is found that the accuracy of the estimated drag depends on the procedure used to detect the edge of the momentum deficit region in the wake. Moreover imposing mass conservation yields the most accurate drag measurements. The drag estimation has little dependence upon the spatialresolution of themeasurement as long as the interrogation window size stays within 5% to 25% of the equivalent diameter of the object cross section. In addition, the drag values obtained with the RoF are compared against the drag estimates from simultaneously acquired power meter data. To assess the agreement between the two approaches in different regimes, drag variations are introduced by different cyclist postures, as well as varying garments. Regardless of the underlying input parameters in the power meter model, both small- and large scale deltas are well captured by both the Ring of Fire technique and the power meter approach. The uncertainty on the average drag measurementsfrom the RoF is within 5%.The second part of this work implements the findings and conclusions from part 1mand presents two applications in speed sports studied with the Ring of Fire. Firstly, themeffect of drafting in cycling is investigated. More precisely, the amount of drag reduction experienced by a trailing cyclists in a tandem formation is investigated at different lateral and longitudinal separations. The longitudinal displacement of the drafters varied between 0.32 m and 0.85 m and the lateral displacement varied between +/- 0.20 m among different runs. The results show that the amount of drag reduction for the trailing rider is mainly caused by the change in inflow conditions and that its aerodynamic advantage decreases with increasing lateral and longitudinal separation between riders, where the lateral distance is found to produce a more rapid effect. Based on these results a model is introduced that predicts the aerodynamic gain of the trailing rider based on his or her position with respect to the leading rider. Validation of the model with data from literature shows that in the near wake the model prediction is in line with literature, with an overestimation of the drag reduction when the longitudinal distance is between 0.1 m and 0.3 m. Secondly, the applicability of the RoF to speed skating is demonstrated. An aerodynamic assessment is presented of two elite skaters, each in two different skating configurations at the ice-rink Thialf in Heerenveen, the Netherlands. Both skaters transit 20 times through the RoF, 10 in each skating configurations. Athlete A skates with two hands on the back and with one arm on the back and one loose. Athlete B skates with both arms loose for all the runs, but was varying his knee and trunk angles. All tests were performed at a nominal speed of 11 m/s. Firstly, the wake velocity fields of skater A, with two hands on the back, are presented throughout five different phases of the skate stroke. Significant variations in the distribution of the velocity deficit downstream of the athlete are observed, which suggest corresponding variations in the skater’s aerodynamic drag. Secondly, average streamwise velocity and vorticity field for all 4 different postures are presented and compared. Finally, the results show that the difference in drag between two arms loose and one arm loose was found to be not statistically significant. Conversely, the optimization of the trunk and knee angles results in a reduction by 7.5% of the skater’s drag.
- Published
- 2022
38. Corticosteroids and Delayed Conversion of SARS-CoV-2 RNA Nasopharyngeal Swabs in Hospitalized Patients With COVID 19 Pneumonia
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Mario Cazzola, Elio Manzillo, Andrea Bianco, Fiorentino Fragranza, Vincenzo Esposito, Vito D'Agnano, Alberto Marra, Roberto Parrella, Rodolfo Punzi, Francesco Scarano, Parrella, R., Marra, A., Scarano, F., Manzillo, E., Esposito, V., Punzi, R., Fragranza, F., D'Agnano, V., Cazzola, M., and Bianco, A.
- Subjects
Pulmonary and Respiratory Medicine ,business.industry ,Medicine ,business ,Molecular biology ,Scientific Letter - Published
- 2021
39. Targeting leptin/CCL3-CCL4 axes in NAFLD/MAFLD: A novel role for BPF in counteracting thalamic inflammation and white matter degeneration.
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Cardamone A, Coppoletta AR, Macrì R, Nucera S, Ruga S, Scarano F, Mollace R, Mollace A, Maurotti S, Micotti E, Carresi C, Musolino V, Gliozzi M, and Mollace V
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- Animals, Male, Mice, Thalamus pathology, Thalamus metabolism, Thalamus drug effects, Chemokine CCL3 metabolism, Diet, High-Fat adverse effects, Plant Extracts pharmacology, Plant Extracts therapeutic use, Inflammation metabolism, Inflammation drug therapy, Leptin metabolism, Non-alcoholic Fatty Liver Disease drug therapy, Non-alcoholic Fatty Liver Disease metabolism, Non-alcoholic Fatty Liver Disease pathology, White Matter drug effects, White Matter pathology, White Matter metabolism
- Abstract
Non-alcoholic fatty liver disease (NAFLD), redefined as Metabolic Associated Fatty Liver Disease (MAFLD), is characterized by an extensive multi-organ involvement. MAFLD-induced systemic inflammatory status and peripheral metabolic alteration lead to an impairment of cerebral function. Herein, we investigated a panel of leptin-related inflammatory mediators as predictive biomarkers of neuroinflammation and evaluated the possible role of Bergamot Polyphenolic Fraction (BPF) in counteracting this MAFLD-induced inflammatory cascade. Male DIAMOND mice were randomly assigned to fed chow diet and tap water or high fat diet with sugar water. Starting from week 16, mice were further divided and treated with vehicle or BPF (50 mg/kg/day), via gavage, until week 30. Magnetic resonance imaging was performed at the baseline and at week 30. Correlation and regression analyses were performed to discriminate the altered lipid metabolism in the onset of cerebral alterations. Steatohepatitis led to an increase in leptin levels, resulting in a higher expression of proinflammatory mediators. The inflammatory biomarkers involved in leptin/CCL3-CCL4 axes were correlated with the altered thalamus energetic metabolism and the white matter degeneration. BPF administration restored leptin level, improved glucose and lipid metabolism, and reduced chronic low-grade inflammatory mediators, resulting in a prevention of white matter degeneration, alterations of thalamus metabolism and brain atrophy. The highlighted positive effect of BPF, mediated by the downregulation of the inflammatory biomarkers involved in leptin/CCL3-CCL4 axes, affording novel elements to candidate BPF for the development of a therapeutic strategy aimed at counteracting MAFLD-related brain inflammation., Competing Interests: Declaration of Competing Interest The authors declare that they have no competing financial interests that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Authors. Published by Elsevier Ltd.. All rights reserved.)
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- 2024
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40. A Systematic Review of the Effect of Polyphenols on Alterations of the Intestinal Microbiota and Shared Bacterial Profiles Between Metabolic Syndrome and Acne.
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Ilari S, Nucera S, Morabito L, Caminiti R, Mazza V, Ritorto G, Ussia S, Passacatini LC, Macrì R, Scarano F, Serra M, Scali E, Maiuolo J, Oppedisano F, Palma E, Muscoli S, Proietti S, Tomino C, Mollace V, and Muscoli C
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- Humans, Bacteria drug effects, Prebiotics administration & dosage, Acne Vulgaris diet therapy, Acne Vulgaris microbiology, Gastrointestinal Microbiome drug effects, Metabolic Syndrome diet therapy, Metabolic Syndrome microbiology, Polyphenols administration & dosage
- Abstract
Introduction: Microbiota, composed of micro-organisms like bacteria, viruses, and non-pathogenic fungi, plays a crucial role in digestion, vitamin production, and protection against dangerous microbes. Several factors, including age, diet, alcohol consumption, stress, environmental microorganisms, and therapies (particularly antibiotics), as well as birth and nursing, could modify the microbiota. Recent research has highlighted its alteration and involvement in a various disease, including metabolic syndrome and acne. This systematic review aimed to identify common biomarkers and microbiota alterations shared between metabolic syndrome and acne, and to explore how the potential prebiotic activities of polyphenols may promote intestinal eubiosis. Materials and methods: A comprehensive search in PubMed and EMBASE resulted in 4142 articles, from which nine studies were selected based on specific criteria after removing duplicates and reviewing abstracts and full texts. All studies correlated the microbiota alteration in both pathologies and the activity of polyphenols in metabolic syndrome. Results: This review suggests that acne may be influenced by some of the same microorganisms involved in metabolic syndrome. While the literature highlights the effectiveness of polyphenols in treating metabolic syndrome, no studies have yet demonstrated their specific impact on acne. Conclusions: The research points to the potential benefits of polyphenols in modulating the microbiota, which could be relevant for individuals with metabolic syndrome. However, due to the limited data available, it was not possible to establish a direct correlation between metabolic syndrome and acne.
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- 2024
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41. Evaluation of the Potential Beneficial Effects of Ferula communis L. Extract Supplementation in Postmenopausal Discomfort.
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Macrì R, Maiuolo J, Scarano F, Musolino V, Fregola A, Gliozzi M, Carresi C, Nucera S, Serra M, Caminiti R, Cardamone A, Coppoletta AR, Ussia S, Ritorto G, Mazza V, Bombardelli E, Palma E, Muscoli C, and Mollace V
- Subjects
- Female, Humans, Middle Aged, Double-Blind Method, Oxidative Stress drug effects, Quality of Life, Cycloheptanes pharmacology, Sexual Behavior drug effects, Body Mass Index, Hydrolyzable Tannins pharmacology, Phytoestrogens pharmacology, Hot Flashes drug therapy, Benzoates, Bridged Bicyclo Compounds, Sesquiterpenes, Ferula chemistry, Plant Extracts pharmacology, Dietary Supplements, Postmenopause
- Abstract
Peri-menopausal discomfort can have a detrimental effect on the physical health of women due to physiological and behavioral changes. Estrogen and progesterone-based hormone therapy can alleviate menopausal symptoms, but estrogen supplementation may have negative health effects. The effectiveness of hormone replacement therapy using natural compounds for peri-menopausal disorders is still uncertain. Evidence from in vivo experiments indicates that Ferula L. extract in ovariectomized rats leads to better sexual behavior. The effect seems to be linked to the phytoestrogenic properties of ferutinin, the primary bioactive compound in the extract. The purpose of this study was to assess the clinical impact of Ferula communis L. extract (titrated at 20% ferutinin, and given at doses of 100 mg/die for 90 days) on the quality of life of 64 menopausal women. The clinical trial was randomized, double-blind, and placebo controlled. Our data showed that Ferula communis L. extract reduced by 67 + 9% all symptoms associated to postmenopausal discomfort and enhanced significantly sexual behavior. In addition, the supplement led to a significant improvement of BMI and oxidative stress decrease in the women who received it, while also keeping platelet aggregation within normal levels. Overall, these results could point to the potential use of supplementation with Ferula communis L. extract to revert or mitigate menopause dysfunction.
- Published
- 2024
- Full Text
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42. Exploring the Role of Bergamot Polyphenols in Alleviating Morphine-Induced Hyperalgesia and Tolerance through Modulation of Mitochondrial SIRT3.
- Author
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Ilari S, Nucera S, Passacatini LC, Scarano F, Macrì R, Caminiti R, Ruga S, Serra M, Giancotti LA, Lauro F, Dagostino C, Mazza V, Ritorto G, Oppedisano F, Maiuolo J, Palma E, Malafoglia V, Tomino C, Mollace V, and Muscoli C
- Subjects
- Animals, Mice, Male, Superoxide Dismutase metabolism, Reactive Oxygen Species metabolism, Spinal Cord drug effects, Spinal Cord metabolism, Antioxidants pharmacology, Analgesics, Opioid pharmacology, Malondialdehyde metabolism, Glutamate-Ammonia Ligase metabolism, Metalloporphyrins pharmacology, Morphine pharmacology, Drug Tolerance, Mitochondria drug effects, Mitochondria metabolism, Hyperalgesia drug therapy, Hyperalgesia chemically induced, Polyphenols pharmacology, Sirtuin 3 metabolism, Oxidative Stress drug effects
- Abstract
Morphine is an important pain reliever employed in pain management, its extended utilize is hindered by the onset of analgesic tolerance and oxidative stress. Long-term morphine administration causes elevated production of reactive oxygen species (ROS), disrupting mitochondrial function and inducing oxidation. Sirtuin 3 (SIRT3), a mitochondrial protein, is essential in modulating ROS levels by regulating mitochondrial antioxidant enzymes as manganese superoxide dismutase (MnSOD). Our investigation focused on the impact of SIRT3 on hyperalgesia and morphine tolerance in mice, as evaluating the antioxidant effect of the polyphenolic fraction of bergamot (BPF). Mice were administered morphine twice daily for four consecutive days (20 mg/kg). On the fifth day, mice received an acute dose of morphine (3 mg/kg), either alone or in conjunction with BPF or Mn (III)tetrakis (4-benzoic acid) porphyrin (MnTBAP). We evaluated levels of malondialdehyde (MDA), nitration, and the activity of SIRT3, MnSOD, glutamine synthetase (GS), and glutamate 1 transporter (GLT1) in the spinal cord. Our findings demonstrate that administering repeated doses of morphine led to the development of antinociceptive tolerance in mice, accompanied by increased superoxide production, nitration, and inactivation of mitochondrial SIRT3, MnSOD, GS, and GLT1. The combined administration of morphine with either BPF or MnTBAP prevented these effects.
- Published
- 2024
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43. Studies on the Comparative Response of Fibers Obtained from the Pastazzo of Citrus bergamia and Cladodes of Opuntia ficus-indica on In Vitro Model of Neuroinflammation.
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Maiuolo J, Liuzzi F, Spagnoletta A, Oppedisano F, Macrì R, Scarano F, Caminiti R, Nucera S, Serra M, Palma E, Muscoli C, and Mollace V
- Abstract
Adhering to a healthy diet has a protective effect on human health, including a decrease in inflammatory diseases due to consuming fiber. The purpose of this manuscript was to obtain and compare two extracts based on fiber (BF and IF-C), derived from two plants particularly present in the Mediterranean region: bergamot ( Citrus bergamia ) and prickly pear ( Opuntia ficus-indica ). The parts used by these plants have been the "pastazzo" for the bergamot and the cladodes for the prickly pear. In addition to in vitro evaluations, the antioxidant activity was also measured on human neurons under inflammatory conditions. Furthermore, the extracts of interest were examined for their effects on the cell cycle and the regulation of pro-apoptotic proteins, caspase 9 and 3, induced by LPS. The results indicated that both extracts had a protective effect against LPS-induced damage, with BF consistently exhibiting superior functionality compared to IF-C. Moreover, the extracts can reduce inflammation, which is a common process of disease. By exploring this avenue, studying the consumption of dietary fiber could enhance our understanding of its positive effects, but additional experiments are needed to confirm this.
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- 2024
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44. Analysis of Endodontic Successes and Failures in the Removal of Fractured Endodontic Instruments during Retreatment: A Systematic Review, Meta-Analysis, and Trial Sequential Analysis.
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Dioguardi M, Dello Russo C, Scarano F, Esperouz F, Ballini A, Sovereto D, Alovisi M, Martella A, and Lo Muzio L
- Abstract
This study presents a systematic review with meta-analysis to evaluate the success rates of endodontic retreatments in teeth where separated instruments are located within the roots. The search and selection of studies were conducted across two databases, SCOPUS and PubMed, as well as the Cochrane Library registry, yielding a total of 1620 records. Following the selection process, 11 studies were included in the systematic review. Overall, out of 1133 retreated teeth, there were 172 failures in instrument removal and 55 perforations. The meta-analysis results indicate that failures are more frequent when instruments are located in the apical third, with a failure rate of 21%, compared to an 8.8% failure rate in the middle/coronal third. The anatomy of the root canals, particularly the location of the separated instruments, significantly influences the success rates.
- Published
- 2024
- Full Text
- View/download PDF
45. The Postbiotic Properties of Butyrate in the Modulation of the Gut Microbiota: The Potential of Its Combination with Polyphenols and Dietary Fibers.
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Maiuolo J, Bulotta RM, Ruga S, Nucera S, Macrì R, Scarano F, Oppedisano F, Carresi C, Gliozzi M, Musolino V, Mollace R, Muscoli C, and Mollace V
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- Humans, Animals, Fatty Acids, Volatile metabolism, Fermentation, Gastrointestinal Microbiome drug effects, Dietary Fiber metabolism, Dietary Fiber pharmacology, Polyphenols pharmacology, Butyrates metabolism
- Abstract
The gut microbiota is a diverse bacterial community consisting of approximately 2000 species, predominantly from five phyla: Firmicutes, Bacteroidetes, Actinobacteria, Proteobacteria, and Verrucomicrobia. The microbiota's bacterial species create distinct compounds that impact the host's health, including well-known short-chain fatty acids. These are produced through the breakdown of dietary fibers and fermentation of undigested carbohydrates by the intestinal microbiota. The main short-chain fatty acids consist of acetate, propionate, and butyrate. The concentration of butyrate in mammalian intestines varies depending on the diet. Its main functions are use as an energy source, cell differentiation, reduction in the inflammatory process in the intestine, and defense against oxidative stress. It also plays an epigenetic role in histone deacetylases, thus helping to reduce the risk of colon cancer. Finally, butyrate affects the gut-brain axis by crossing the brain-blood barrier, making it crucial to determine the right concentrations for both local and peripheral effects. In recent years, there has been a significant amount of attention given to the role of dietary polyphenols and fibers in promoting human health. Polyphenols and dietary fibers both play crucial roles in protecting human health and can produce butyrate through gut microbiota fermentation. This paper aims to summarize information on the key summits related to the negative correlation between intestinal microbiota diversity and chronic diseases to guide future research on determining the specific activity of butyrate from polyphenols and dietary fibers that can carry out these vital functions.
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- 2024
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46. Cladodes of Opuntia ficus-indica (L.) Mill. Possess Important Beneficial Properties Dependent on Their Different Stages of Maturity.
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Maiuolo J, Nucera S, Serra M, Caminiti R, Oppedisano F, Macrì R, Scarano F, Ragusa S, Muscoli C, Palma E, and Mollace V
- Abstract
Opuntia ficus-indica (L.) Mill. belongs to the Cactaceae family and the genus Opuntia; it is a succulent plant that adapts to extreme climatic conditions. The aerial part of the plant consists of the cladodes, morphological changes of branches that appear green, are covered with thorns, and are essential to reduce excessive perspiration of water. The composition of cladodes is very varied, and the main constituents are water, fibers, polysaccharides, proteins, fatty acids, vitamins, sterols, minerals, and polyphenols. Polyphenols are responsible for many beneficial activities for human health, such as antioxidant, anti-inflammatory, anticancer, and nutritional properties. The purpose of this manuscript was to compare the properties of cladodes belonging to the same plant but with different stages of maturity. Relative extracts were tested both in vitro and on a cell line and antioxidant and anti-apoptotic properties were found. The antioxidant activity was tested by the Oxygen Radical Absorbance Capacity (ORAC) test, the 1,1-diphenyl-2-picrylhydrazil (DPPH) test, and the measurement of cellular accumulation of reactive oxygen species (ROS). Anti-apoptotic activity was evaluated by the annexin/PI assay and measurement of caspases 9 and 3 expression. The results obtained showed that the extracts considered possess antioxidant and anti-apoptotic properties. However, the different stages of maturity of cladodes are essential for the performance of both functions. In addition, important variations were made in the dissolution of the extracts that brought greater safety in their use. In conclusion, this manuscript provides further information on cladodes of Opuntia ficus-indica , which can be used as adjuvants in many human pathologies.
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- 2024
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47. Impact of liver fibrosis on COVID-19 in-hospital mortality in Southern Italy.
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Galiero R, Loffredo G, Simeon V, Caturano A, Vetrano E, Medicamento G, Alfano M, Beccia D, Brin C, Colantuoni S, Di Salvo J, Epifani R, Nevola R, Marfella R, Sardu C, Coppola C, Scarano F, Maggi P, Calabrese C, De Lucia Sposito P, Rescigno C, Sbreglia C, Fraganza F, Parrella R, Romano A, Calabria G, Polverino B, Pagano A, Numis F, Bologna C, Nunziata M, Esposito V, Coppola N, Maturo N, Nasti R, Di Micco P, Perrella A, Adinolfi LE, Chiodini P, Di Domenico M, Rinaldi L, and Sasso FC
- Subjects
- Humans, Italy epidemiology, Female, Male, Middle Aged, Retrospective Studies, Aged, Severity of Illness Index, Aged, 80 and over, Hospitalization statistics & numerical data, Adult, COVID-19 mortality, COVID-19 epidemiology, COVID-19 pathology, Liver Cirrhosis mortality, Liver Cirrhosis pathology, Liver Cirrhosis virology, Hospital Mortality, SARS-CoV-2 isolation & purification
- Abstract
Background & Aims: SARS-Cov-2 infection manifests as a wide spectrum of clinical presentation and even now, despite the global spread of the vaccine, contagiousness is still elevated. The aim of the study was the evaluation of the impact of liver fibrosis assessed by FIB-4 and liver impairment, assessed by cytolysis indices, on intrahospital mortality in COVID-19 subjects., Methods: This is a retrospective observational cohort study, which involved 23 COVID Hospital Units in Campania Region, Italy. Exposure variables were collected during hospital admission and at discharge. According to FIB-4 values, we subdivided the overall population in three groups (FIB-4<1.45; 1.45
3.25), respectively group 1,2,3., Results: At the end of the study, 938 individuals had complete discharged/dead data. At admission, 428 patients were in group 1 (45.6%), 387 in group 2 (41.3%) and 123 in group 3 (13.1%). Among them, 758 (81%) subjects were discharged, while the remaining 180 (19%) individuals died. Multivariable Cox's regression model showed a significant association between mortality risk and severity of FIB-4 stages (group 3 vs group 1, HR 2.12, 95%CI 1.38-3.28, p<0.001). Moreover, Kaplan-Meier analysis described a progressive and statistically significant difference (p<0.001 Log-rank test) in mortality according to FIB-4 groups. Among discharged subjects, 507 showed a FIB-4<1.45 (66.9%, group 1), 182 a value 1.45 3.25 (9.0%, group 3). Among dead subjects, 42 showed a FIB-4<1.45 (23.3%, group 1), 62 a value 1.45 3.25 (42.3%, group 3)., Conclusions: FIB-4 value is significantly associated with intrahospital mortality of COVID-19 patients. During hospitalization, particularly in patients with worse outcomes, COVID-19 seems to increase the risk of acute progression of liver damage., Competing Interests: The authors have declared that no competing interests exist., (Copyright: © 2024 Galiero et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.) - Published
- 2024
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48. Trail pheromone identification in the ant Crematogaster scutellaris.
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Scarano F, Giannetti D, Trenti F, Giacomazzi F, Vigna J, Guella G, Grasso DA, and Haase A
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- Animals, Behavior, Animal, Feeding Behavior, Pheromones analysis, Ants physiology
- Abstract
In this work, we identified the trail pheromone of the ant Crematogaster scutellaris. We combined gas chromatography-mass spectrometry analysis of extracts from the hind tibia, the location of the respective glands, with automated trail following assays. The study found tridecan-2-ol to be the strongest discriminator between hind tibia and other body part extracts. Tridecan-2-ol elicited trail-following behaviour at concentrations of 1 ng/µL. A separation of the enantiomers showed responses to (R)-tridecan-2-ol already at 0.001 ng/µL and only at a 1000-fold higher concentration for (S)-tridecan-2-ol, suggesting that only the R enantiomer is used by C. scutellaris in its natural environment. We also found strong behavioural responses to 2-dodecanol, a substance that was not detectable in the hind tibia extract of C. scutellaris, but which has been reported to be the trail pheromone of the related species C. castanea. We discuss the contribution of these results to the 'dissection and reconstruction' of strategies and mechanisms underlying the social organization of ants., (© 2024. The Author(s).)
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- 2024
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49. Frailty and HIV-associated neurocognitive disorder are only modestly associated.
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González-Scarano F, Napravnik S, and Vance DE
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- Humans, Neurocognitive Disorders, Neuropsychological Tests, HIV Infections complications, HIV Infections psychology, Frailty complications, AIDS Dementia Complex
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- 2024
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50. The dangerous "West Coast Swing" by hyperglycaemia and chronic stress in the mouse hippocampus: Role of kynurenine catabolism.
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Gliozzi M, Coppoletta AR, Cardamone A, Musolino V, Carresi C, Nucera S, Ruga S, Scarano F, Bosco F, Guarnieri L, Macrì R, Mollace R, Belzung C, and Mollace V
- Subjects
- Animals, Mice, Humans, Kynurenine, Hippocampus, Hyperglycemia, Neurodegenerative Diseases, Depressive Disorder, Major
- Abstract
Growing epidemiological studies highlight a bi-directional relationship between depressive symptoms and diabetes mellitus. However, the detrimental impact of their co-existence on mental health suggests the need to treat this comorbidity as a separate entity rather than the two different pathologies. Herein, we characterized the peculiar mechanisms activated in mouse hippocampus from the concurrent development of hyperglycaemia, characterizing the different diabetes subtypes, and chronic stress, recognized as a possible factor predisposing to major depression. Our work demonstrates that kynurenine overproduction, leading to apoptosis in the hippocampus, is triggered in a different way depending on hyperglycaemia or chronic stress. Indeed, in the former, kynurenine appears produced by infiltered macrophages whereas, in the latter, peripheral kynurenine preferentially promotes resident microglia activation. In this scenario, QA, derived from kynurenine catabolism, appears a key mediator causing glutamatergic synapse dysfunction and apoptosis, thus contributing to brain atrophy. We demonstrated that the coexistence of hyperglycaemia and chronic stress worsened hippocampal damage through alternative mechanisms, such as GLUT-4 and BDNF down-expression, denoting mitochondrial dysfunction and apoptosis on one hand and evoking the compromission of neurogenesis on the other. Overall, in the degeneration of neurovascular unit, hyperglycaemia and chronic stress interacted each other as the partners of a "West Coast Swing" in which the leading role can be assumed alternatively by each partner of the dance. The comprehension of these mechanisms can open novel perspectives in the management of diabetic/depressed patients, but also in the understanding the pathogenesis of other neurodegenerative disease characterized by the compromission of hippocampal function., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Authors. Published by Elsevier Ltd.. All rights reserved.)
- Published
- 2024
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