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Biting midges are insects which annoy horses as they cause discomfort and by the sucking of blood, they cause painful lesions that, in certain cases, can trigger acute allergic reactions. They also act as potential vectors of many pathogens of bacterial, viral and parasitic origin. The aim of this study was to investigate seasonal dynamics, abundance and species composition of biting midges, and implementing protection options at the Equestrian centre of the University of Veterinary Medicine and Pharmacy (UVMP) in Košice in the years 2021 and 2022. During this time period (of 2021 and 2022), we captured 4797 biting midges. Our results showed that in 2021 the most frequently captured species were C. obsoletus/C. scoticus (prevalence 86.6 %), followed by C. punctatus and C. pulicaris (prevalence 5.37 % and 3.86 %, resp.). In addition, C. lupicaris, C. newsteadi, C. furcillatus, C. festivipennis, C. slovacus, C. tauricus, C. clastrieri, C. dewulfi, C. fagineus and C. circumscriptus were identified as the minority species. In this year, the highest abundance of these insects (1758 biting midges) was observed at the end of June. In 2022, we detected a clear dominance of C. obsoletus/C. scoticus (prevalence 97.0 %) and the highest number of biting midges (655) was captured in mid-May). In this year, the minority species included, C. pulicaris, C. punctatus and C. lupicaris. The identification of the host blood showed that man (Homo sapiens) was the dominant host, namely of 80 % of the examined biting midges of species C. obsoletus/C. scoticus, C. dewulfi, C. punctatus and C. festivipennis. In one female of species C. fagineus we identified the host blood from horses (Equus caballusi). In 2022, we detected the blood of a domestic rabbit (Oryctolagus cuniculus) in the species of C. obsoletus/C. scoticus.

This study was conducted with the aim of the molecular identification of the protozoan parasite Cryptosporidium spp. in calves in the early stage of their development on a dairy farm in Eastern Slovakia. Twenty-five Holstein and Holstein cross calves were included in the study and monitored from their birth to the fifth week of life (1–5 weeks). Fresh fecal samples were collected from the same group of calves each week, except during the fourth week, and with the exception of Sample 8. All samples were analyzed using the Ziehl–Neelsen staining method and coproantigen was tested using the ELISA test as the screening method. Using the ELISA method, the highest incidence of cryptosporidiosis was observed in the second week of life of the calves, while the antigen was detected in 21 (91.6%) calves. Using the Ziehl–Neelsen staining method, the highest incidence was also observed in the second week, with an incidence rate of 62.5%. Positive isolates confirmed by the ELISA test were molecularly characterized. The species and subtypes of Cryptosporidium in the positive isolates were identified using PCR and the sequence analysis of the small subunit of the ribosomal 18S RNA (ssu rRNA) and the 60 kDa glycoprotein (gp60) genes of the parasite. The sequence analysis of 29 isolates at the 18S rRNA loci confirmed the presence of two species—Cryptosporidium parvum and Cryptosporidium ryanae. Out of 29 isolates, 25 were assigned to the species C. parvum, with the gp60 locus identified as genotype IIaA17G1R1. Among the individual animal groups, calves are the most common reservoirs of the C. parvum zoonotic species. This disease has significant public health implications as contact with livestock and their feces and working with barn manure are major sources of infection, not only for other animals but also for humans. [ABSTRACT FROM AUTHOR]