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Your search keyword '"Sebastián Ramos‐Onsins"' showing total 2 results
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2 results on '"Sebastián Ramos‐Onsins"'

1. Whole genome scanning of a Mediterranean basin hotspot collection provides new insights into olive tree biodiversity and biology

Author

Christos Bazakos, Konstantinos G. Alexiou, Sebastián Ramos‐Onsins, Georgios Koubouris, Nikolaos Tourvas, Aliki Xanthopoulou, Ifigeneia Mellidou, Theodoros Moysiadis, Ioanna‐Theoni Vourlaki, Ioannis Metzidakis, Chrysi Sergentani, Ioanna Manolikaki, Michail Michailidis, Adamantia Pistikoudi, Alexios Polidoros, George Kostelenos, Filippos Aravanopoulos, Athanassios Molassiotis, Ioannis Ganopoulos, Producció Vegetal, and Genòmica i Biotecnologia

Subjects
Genetics, Cell Biology, Plant Science
Abstract

Olive tree (Olea europaea L. subsp. europaea var. europaea) is one of the most important species of the Mediterranean region and one of the most ancient species domesticated. The availability of whole genome assemblies and annotations of olive tree cultivars and oleaster (O. europaea subsp. europaea var. sylvestris) has contributed to a better understanding of genetic and genomic differences between olive tree cultivars. However, compared to other plant species there is still a lack of genomic resources for olive tree popula-tions that span the entire Mediterranean region. In the present study we developed the most complete genomic variation map and the most comprehensive catalog/resource of molecular variation to date for 89 olive tree genotypes originating from the entire Mediterranean basin, revealing the genetic diversity of this commercially significant crop tree and explaining the divergence/similarity among different variants. Addi-tionally, the monumental ancient tree ‘Throuba Naxos’ was studied to characterize the potential origin or routes of olive tree domestication. Several candidate genes known to be associated with key agronomic traits, including olive oil quality and fruit yield, were uncovered by a selective sweep scan to be under selection pressure on all olive tree chromosomes. To further exploit the genomic and phenotypic resources obtained from the current work, genome-wide association analyses were performed for 23 morphological and two agronomic traits. Significant associations were detected for eight traits that provide valuable candidates for fruit tree breeding and for deeper understanding of olive tree biology. info:eu-repo/semantics/publishedVersion

Published
2023
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2. Copy Number Variation on ABCC2-DNMBP Loci Affects the Diversity and Composition of the Fecal Microbiota in Pigs

Author

Yuliaxis Ramayo-Caldas, Daniel Crespo-Piazuelo, Jordi Morata, Olga González-Rodríguez, Cristina Sebastià, Anna Castello, Antoni Dalmau, Sebastian Ramos-Onsins, Konstantinos G. Alexiou, Josep M. Folch, Raquel Quintanilla, and Maria Ballester

Subjects
diversity, microbiota, modulators, porcine, structural variants, Microbiology, QR1-502
Abstract

ABSTRACT Genetic variation in the pig genome partially modulates the composition of porcine gut microbial communities. Previous studies have been focused on the association between single nucleotide polymorphisms (SNPs) and the gut microbiota, but little is known about the relationship between structural variants and fecal microbial traits. The main goal of this study was to explore the association between porcine genome copy number variants (CNVs) and the diversity and composition of pig fecal microbiota. For this purpose, we used whole-genome sequencing data to undertake a comprehensive identification of CNVs followed by a genome-wide association analysis between the estimated CNV status and the fecal bacterial diversity in a commercial Duroc pig population. A CNV predicted as gain (DUP) partially harboring ABCC2-DNMBP loci was associated with richness (P = 5.41 × 10−5, false discovery rate [FDR] = 0.022) and Shannon α-diversity (P = 1.42 × 10−4, FDR = 0.057). The in silico predicted gain of copies was validated by real-time quantitative PCR (qPCR), and its segregation, and positive association with the richness and Shannon α-diversity of the porcine fecal bacterial ecosystem was confirmed in an unrelated F1 (Duroc × Iberian) cross. Our results advise the relevance of considering the role of host-genome structural variants as potential modulators of microbial ecosystems and suggest the ABCC2-DNMBP CNV as a host-genetic factor for the modulation of the diversity and composition of the fecal microbiota in pigs. IMPORTANCE A better understanding of the environmental and host factors modulating gut microbiomes is a topic of greatest interest. Recent evidence suggests that genetic variation in the pig genome partially controls the composition of porcine gut microbiota. However, since previous studies have been focused on the association between single nucleotide polymorphisms and the fecal microbiota, little is known about the relationship between other sources of genetic variation, like the structural variants and microbial traits. Here, we identified, experimentally validated, and replicated in an independent population a positive link between the gain of copies of ABCC2-DNMBP loci and the diversity and composition of pig fecal microbiota. Our results advise the relevance of considering the role of host-genome structural variants as putative modulators of microbial ecosystems and open the possibility of implementing novel holobiont-based management strategies in breeding programs for the simultaneous improvement of microbial traits and host performance.

Published
2023
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