Search

Streptomyces anulatus is a typical representative of the Streptomyces genus synthesizing a large number of biologically active compounds. In this study, the draft genome of Streptomyces anulatus, strain K-31 is presented, generated from Illumina reads by SPAdes software. The size of the assembled genome was 8.548838 Mb. Annotation of the S. anulatus genome assembly identified C. hemipterus genome 7749 genes, including 7149 protein-coding genes and 92 RNA genes. This genome will be helpful to further understand Streptomyces genetics and evolution and can be useful for obtained biological active compounds. Dataset: The raw genome sequencing data of Illumina MiSeq were submitted to NCBI SRA database in FASTQ format with BioSample: SAMN35538655, under BioProject PRJNA977889. The assembled genome available in NCBI GeneBank under CP127386.1. Dataset License: CC0 [ABSTRACT FROM AUTHOR]

Inulinase is an enzyme that catalyzes inulin to d‐fructose. This enzyme can be extracted from plants, but it is difficult to obtain it in large quantities, so its production cost is high. Therefore, microbial inulinase has great potential for industrial needs. In the last decade, there have been very few reports on actinobacterial inulinases, especially on purification and characterization of inulinase process extraction. This study aims to select actinomycetes that possess high inulinase activity from the soil. To screen inulinase‐producing bacteria, modified Czapex‐Dox agar supplemented with 1% inulin powder was used. The most effective isolate was Streptomyces sp. EFBO8, morphological and genotypic identification methods, confirmed that the strain is Streptomyces anulatus and that its nucleotide sequence has been deposited in GenBank under accession number OQ073700. To optimize inulinase production, kinetics were performed by using S. anulatus strain, which proved to be most productive with a value of 24,024 EU/mL. The enzyme was purified from the culture filtrate by precipitation with ammonium sulfate (NH4)2SO4, followed by column chromatography Sephadex (G‐50) separation. Purified protein has a molecular mass of 3331.83 Da. [ABSTRACT FROM AUTHOR]

Streptomyces anulatus is a typical representative of the Streptomyces genus synthesizing a large number of biologically active compounds. In this study, the draft genome of Streptomyces anulatus, strain K-31 is presented, generated from Illumina reads by SPAdes software. The size of the assembled genome was 8.548838 Mb. Annotation of the S. anulatus genome assembly identified C. hemipterus genome 7749 genes, including 7149 protein-coding genes and 92 RNA genes. This genome will be helpful to further understand Streptomyces genetics and evolution and can be useful for obtained biological active compounds.