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Possible use of Thioflavin T based fluorescent molecular rotors as temperature sensors was assessed in this work. Fluorescent properties of Thioflavin T and its derivative 6-Me-BTA-2C in 99% glycerol were studied using steady-state and time-resolved fluorescence spectroscopy methods. For Thioflavin T in glycerol it has been found that temperature growth from 261 K to 353 K results in ~ 3 orders of magnitude increase of non-radiative decay rate constant knr for the excited state of the molecule. Fluorescence decay studies by time-correlated single photon counting method showed that fluorescence decay kinetics of Thioflavin T in glycerol can be used for temperature measurements in the range 260-290 K. For 6-Me-BTA-2C molecule the range of the maximal sensitivity was shifted to higher temperatures (280-320 K). The obtained results about fluorescent properties and decay kinetics of Thioflavin T based dyes in highly viscous media can be used to develop nanoscale temperature sensors. Keywords: temperature sensor, fluorescent molecular rotor, nanothermometry, thioflavin T, TICT.