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Maritime studies, encompassing a range of disciplines, increasingly rely on advanced data analytics, particularly in the context of navigation. As technology advances, the statistical averaging of large datasets has become a critical component of these analyses. However, recent studies have highlighted discrepancies between statistical predictions and observable realities, especially in high-stress environments like port approach procedures conducted by marine pilots. This study analyzed physiological responses recorded during simulation exercises involving experienced marine pilots. The focus was not on the specific outcomes of the simulations but on the potential faults arising from conventional statistical signal processing, particularly mean-centered approaches. A large dataset of signals was generated, including one signal with a dominant characteristic intentionally designed to introduce imbalance, mimicking the uneven distribution of real-world data. Initial analysis suggested that the average physiological response of the pilots followed an S-shaped curve, indicative of a psycho-physiological reaction to stress. However, further post hoc analysis revealed that this pattern was primarily influenced by a single participant's data. This finding raises concerns about the generalizability of the S-curve as a typical stress response in maritime pilots. The results underscore the limitations of relying solely on conventional statistical methods, such as mean-centered approaches, in interpreting complex datasets. The study calls into question the validity of standardizing data interpretations based on dominant characteristic curves, particularly in environments as intricate as maritime navigation. The research highlights the need for a re-evaluation of these methods to ensure more reliable and nuanced conclusions in maritime studies. This study contributes to the ongoing discourse on data interpretation in maritime research, emphasizing the critical need to re-assess conventional statistical signal processing techniques. By recognizing the potential pitfalls in data generalization, the study advocates for more robust analytical approaches to better capture the complexities of real-world maritime challenges., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2024 Košir, Svetina, Perkovič, Dimc, Brcko and Žagar.)

Background: In patients who require venom immunotherapy (VIT), there is a need to identify underlying mast cell (MC) disorders since these may affect the risk and severity of future sting reactions and the long-term effectiveness of VIT., Methods: 1319 individuals with Hymenoptera venom allergy (HVA) who needed VIT from referral centers in Slovenia, Austria, Croatia, and Poland underwent examination for KIT p.D816V in peripheral blood leukocytes (PBL) using a highly sensitive PCR test and tryptase genotyping by digital droplet PCR. We also included 183 control individuals with large local reactions (LLRs) to Hymenoptera stings and with asymptomatic sensitization to Hymenoptera venoms., Results: 285 of 1319 individuals recommended for VIT (21.6%) were positive for KIT p.D816V in PBL, preferably those who present with severe reaction (33.9% [n = 207 of 610] with Ring-Messmer grade 3-4 vs. 11% [n = 78 of 709] with Grade 1-2; p < .0001), whereas only 1.3% (n = 2 of 152) of controls with LLR and none with asymptomatic sensitization (n = 31) had KIT p.D816V. KIT p.D816V allelic burden was higher in those with severe reaction (median 0.018% [n = 207] in Grade 3-4 vs. 0.001% [n = 78] in Grade 1-2; p < .0001), and the majority had normal baseline serum tryptase levels (69% [n = 196 of 285]). All KIT p.D816V-positive individuals (n = 41) who underwent bone marrow (BM) biopsy were found to have underlying clonal diseases, principally BM mastocytosis. HαT was also associated with severe HVA and symptoms (p < .01), and remarkably, 31.0% (n = 31 of 100) were found to have concomitant KIT p.D816V. Concomitant HαT and KIT p.D816V showed an additive effect, and having both was associated with the highest risk for severe HVA, even higher than having either HαT or KIT p.D816V alone (OR = 3.8; p < .01)., Conclusions: By employing prospective universal tryptase genotyping and examination for KIT p.D816V in PBL in large HVA populations, we have demonstrated a high burden of clonal MC disorders and HαT in patients who require VIT., (© 2024 The Authors. Allergy published by European Academy of Allergy and Clinical Immunology and John Wiley & Sons Ltd.)

Hereditary α tryptasemia (HαT) is an autosomal dominant trait characterized by increased TPSAB1 copy number (CN) encoding α-tryptase. The determination of HαT is being discussed as an important biomarker to be included in risk assessment models and future diagnostic algorithms for patients with mastocytosis and anaphylaxis. Due to the complex genetic structure at the human tryptase locus, genetic testing for tryptase gene composition is presently notably limited and infrequently pursued. This study aimed to develop, optimise and validate a multiplex droplet digital PCR (ddPCR) assay that can reliably quantify α- and β-tryptase encoding sequences in a single reaction. To optimise the ddPCR conditions and establish an amplitude-based multiplex ddPCR assay, additional primers and probes, a thermal gradient with varying annealing temperatures, different primers/probe concentrations, and various initial DNA quantities were tested. Results obtained from all 114 samples analysed using multiplex ddPCR were identical to those obtained through the use of original duplex assays. Utilizing this multiplex ddPCR assay, in contrast to conducting distinct duplex ddPCRs, presents noteworthy benefits for tryptase genotyping. These advantages encompass a substantial threefold decrease in material costs and considerable time savings. Consequently, this approach exhibits high suitability and particularly captures interest for routine clinical implementation., (© 2024. The Author(s).)

The main objective of the present study was to investigate factors related to seafarers' mental health. A sample of seafarers from 12 countries participated in the study. A list of stressors was used to assess both perception of exposure to these stressors and their subjective significance. The Symptom Checklist (SCL-90) was used to assess seafarers' mental health on 5 of 9 subscales: Depression, Anxiety, Hostility, Interpersonal Sensitivity, and Somatisation. Three significant findings emerged from the analyses. The first was that 3 types of stressors contribute significantly to mental health problems: (1) environmental factors (eg, vibration), (2) social problems (eg, bullying, homesickness, working alone), and (3) health problems (eg, physical injuries, viruses, and the illnesses). The second finding was that both stress and mental health issues determine seafarers' motivation for their work and their consideration regarding leaving the maritime industry. The third finding was that factors contributing to seafarers' consideration of leaving the industry were mainly related to social stressors such as isolation from family and friends, cultural differences at work, demands from supervisors, and bullying. Factors such as bad weather, working shifts, length of employment contract or a ban on disembarkment in ports were found to be relatively less important for seafarers as factors toward considering leaving the industry. The implications of these findings are discussed., Competing Interests: Declaration of Conflicting InterestsThe author(s) declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.

The study examined the use of maladaptive coping strategies such as conflict, avoidance and self-blame in predicting anxiety and depression in couples undergoing IVF procedure. The target group consisted of 80 couples who started the IVF treatment at the Division of Obstetrics and Gynaecology, Ljubljana's University Medical Centre in Slovenia. The participants completed three scales: S/T Anxiety Inventory, CES Depression Scale, and Marital Coping Inventory. The results showed increased rates of depression compared to the normative population; 18% of women and 14% of men reported mild to moderate depression or the possibility of major depression. Women showed higher anxiety scores than men. Coping strategies predicted both depression and anxiety, with self-blame being the most toxic one. The study showed that self-blame is a coping strategy that should be properly addressed in the couples at the very outset of IVF treatment, to decrease emotional disorders and increase the likability of a succesful IVF medical procedures.

The purpose of this study is to analyse data from the marine pilots' bio-sensor readings to determine how experience affects their biometrical response during the port approach. The experiences play a significant role in the participant's decision-making process and correlate with the repetitions. Through the repetitions of the experimental task, the participants gain experience, which correlates with the biometrical response, e.g., heart rate, electrodermal activity, etc. After exposing the two experience-distinct groups of participants to the same simulated port-approaching task, their collected biometric data is analysed and discussed. The results show that biometrical readings of the less experienced participants typically vary compared to that of the experienced participants, who take the simulated task more seriously. The study also yields insight into the workload process, involving disturbing factors during the task.

We propose a new exercise, the abdominal rise on the ball, to replace the traditional crunch in exercise programs. The aim of this study is to compare the activity of the abdominal muscles when performing an ARB with the same activity when performing a traditional crunch. Twenty healthy adults participated in the study. Surface electromyography (EMG) was recorded from the upper and lower rectus abdominis (URA, LRA), internal oblique (IO), external oblique (EO), transversus abdominis (TrA), and erector spinae (ES). EMG values were normalized to maximal voluntary isometric contraction. A paired t -test, nonparametric Wilcoxon test and correlation coefficient were used for statistical analysis. The normalized EMG values of EO, TrA and ES, were statistically significantly higher during the abdominal rise on the ball compared to the traditional crunch, while URA, LRA and IO were significantly lower during the abdominal rise on the ball compared to the traditional crunch. TrA, EO and IO are sufficiently activated during an abdominal rise on a ball, so the exercise could be deemed effective for strengthening these muscles.