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Parameters for wound age estimation are essential in forensic pathology but difficult to assess. With this retrospective study, wound age parameters in canine skin wounds were assessed with archive material of known age. Haematoxylin and eosin (HE) staining as standard, Prussian blue, Van Gieson (VG) and multiple other special stains were used as well as various immunohistochemistry (IHC) tests. Collagen fibre formation examination included HE staining but also immunolabelling for elastin and collagen I. Collagen fibre formation was further assessed by polarization in HE and VG. HE staining, erythrophagocytosis and presence of haemoglobin decay products in the Prussian blue stained-slides proved to be useful tools in wound age estimation in the early phase of wound repair of less than 5 days. The earliest detection of newly formed collagen was possible in 5-day-old wounds with HE and VG staining. Collagen I reactivity by IHC was weak at this time point, moderate to strong in the lesions older than 10 days and up to 30 days and strong in the lesions older than 3 months. Very slight polarization of collagen fibres was observed at 6 days, becoming stronger in wound lesions of 10-14 days and up to 30 days and reaching the same intensity as normal tissue in a 3-month-old lesion. The morphology of newly formed collagen fibres, utilizing HE and VG staining and polarization as well as collagen I IHC, which proved to be the most important parameters, correlated with distinguishable time points in the available cases of known wound age., Competing Interests: Declaration of competing interests The authors declared no conflicts in relation to the research, authorship and publication of this paper., (Copyright © 2024 The Author(s). Published by Elsevier Ltd.. All rights reserved.)

Rotavirus (RV) infections are the most important viral cause of diarrhea in piglets in Switzerland and are thought to cause substantial economic losses to the pig industry. However, no data are available on the occurrence and dynamics of the main porcine RV species, namely RVA, RVB, and RVC, and the diversity of the circulating strains. We therefore tested fecal samples from a cross-sectional ( n = 95) and a longitudinal ( n = 48) study for RVA, RVB, and RVC by real-time RT-PCR and compared the results of the cross-sectional study to postmortem findings. In addition, eight samples were fully genotyped by using next-generation sequencing. In the cross-sectional study, triple RV infections significantly correlated with diarrhea and wasting and were most frequent in the weaned age group. In the longitudinal study, the shedding of RV peaked one week after weaning and decreased thereafter. Here, mainly double infections were seen, and only a few animals showed diarrhea. The full-genome sequencing revealed a genotype pattern similar to other European countries and, importantly, co-infection by up to four RVA strains. Our results imply that the weaning of piglets may trigger not only RV shedding but facilitate co-infection of multiple RV species and strains in the same host.