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In this study, fourteen types of biochar produced using seven biomasses at temperatures 300 °C and 600 °C were screened for phenolics (furfural and hydroxymethylfurfural (HMF)) removal. Eucheuma spinosum biochar (EB-BC 600) showed higher adsorption capacity to furfural (258.94±3.2 mg/g) and HMF (222.81±2.3 mg/g). Adsorption kinetics and isotherm experiments interpreted that EB-BC 600 biochar followed the pseudo-first-order kinetic and Langmuir isotherm model for both furfural and HMF adsorption. Different hydrolysates were detoxified using EB-BC 600 biochar and used as feedstock for engineered Escherichia coli. An increased polyhydroxyalkanoates (PHA) production with detoxified barley biomass hydrolysate (DBBH: 1.71±0.07 g PHA/L), detoxified miscanthus biomass hydrolysate (DMBH: 0.87±0.03 g PHA/L) and detoxified pine biomass hydrolysate (DPBH: 1.28±0.03 g PHA/L) was recorded, which was 2.8, 6.4 and 3.4 folds high as compared to undetoxified hydrolysates. This study reports the mechanism involved in furfural and HMF removal using biochar and valorization of hydrolysate into PHA.

Bacteria can evade antibiotics by acquiring resistance genes, as well as switching to a non-growing dormant state without accompanying genetic modification. Bacteria in this quiescent state are called persisters, and this non-inheritable ability to withstand multiple antibiotics is referred to as antibiotic tolerance. Although all bacteria are considered to be able to form antibiotic-tolerant persisters, the antibiotic tolerance of extremophilic bacteria is poorly understood. Previously, we identified the psychrotolerant bacterium

The present study deals with the utilization of lignocellulosic hydrolysate-based carbon source for exopolysaccharide (EPS) production using newly reported marine Echinicola sediminis BBL-M-12. This bacterium produced 7.56 g L

The existing study deals with adsorptive removal of the endocrine-disrupting chemical bisphenol-A and toxic azo dye solvent black-3 from single and binary solutions. These two chemicals are commonly used as an additive in the synthetic plastic industries. Among the tested twenty pristine and modified biochars, the pristine pinecone biochar produced at 750 °C revealed greater bisphenol-A removal. Simulation of the experimental data obtained for bisphenol-A and dye removal from the single-component solution offered a best-fit to Elovich (R

The cellular components of Akkermansia muciniphila are considered potential biotherapeutics for the improvement of obesity, diabetes, and metabolic diseases. However, the molecular-based mechanism of A. muciniphila for treatment of obesity, which can provide important evidence for human research, has rarely been explored. Here, we applied integrative multiomics approaches to investigate the underlying molecular mechanism involved in obesity treatment by A. muciniphila. First, the treatment with a cell lysate of A. muciniphila reduced lipid accumulation in 3T3-L1 cells and downregulated the mRNA expression of proteins involved in adipogenesis and lipogenesis. Our proteomic results revealed that A. muciniphila decreased the expression of proteins involved in fat cell differentiation, fatty acid metabolism, and energy metabolism in adipocytes. Moreover, A. muciniphila significantly reduced the level of metabolites related to glycolysis, the TCA cycle, and ATP in adipocytes. Interestingly, serine protease inhibitor A3 (SERPINA3) homologs were overexpressed in the 3T3-L1 cells treated with A. muciniphila. Small interfering RNA (siRNA) transfection demonstrated that A. muciniphila upregulates SERPINA3G expression and inhibits lipogenesis in adipocytes. Taken together, our multiomics-based approaches enabled to uncover the molecular mechanism of A. muciniphila for treatment of obesity and provide potent anti-lipogenic agents.