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2. Virological characteristics of a SARS-CoV-2-related bat coronavirus, BANAL-20-236

Author

Sawa, Hirofumi, Mizuma, Keita, Li, Jingshu, Mimura, Yume, Ohari, Yuma, Tsubo, Tomoya, Ferdous, Zannatul, Shishido, Kenji, Mohri, Hiromi, Iida, Miki, Tsujino, Shuhei, Misawa, Naoko, Usui, Kaoru, Saikruang, Wilaiporn, Lytras, Spyridon, Kawakubo, Shusuke, Nishumura, Luca, Mendoza Tolentino, Jarel Elgin, Li, Wenye, Yo, Maximilian Stanley, Horinaka, Kio, Suganami, Mai, Chiba, Mika, Yoshimura, Ryo, Yasuda, Kyoko, Iida, Keiko, Strange, Adam Patrick, Ohsumi, Naomi, Tanaka, Shiho, Ogawa, Eiko, Okumura, Kaho, Fukuda, Tsuki, Osujo, Rina, Yoshida, Isao, Nakagawa, So, Takaori-Kondo, Akifumi, Shirakawa, Kotaro, Nagata, Kayoko, Nomura, Ryosuke, Horisawa, Yoshihito, Tashiro, Yusuke, Kawai, Yugo, Nakata, Yoshitaka, Futatsusako, Hiroki, Sakamoto, Ayaka, Yasuhara, Naoko, Hashiguchi, Takao, Suzuki, Tateki, Kimura, Kanako, Sasaki, Jiei, Nakajima, Yukari, Yajima, Hisano, Irie, Takashi, Kawabata, Ryoko, Sasaki-Tabata, Kaori, Shimizu, Ryo, Monira Begum, M.S.T., Jonathan, Michael, Mugita, Yuka, Leong, Sharee, Takahashi, Otowa, Ichihara, Kimiko, Ueno, Takamasa, Motozono, Chihiro, Toyoda, Mako, Saito, Akatsuki, Kosaka, Anon, Kawano, Miki, Matsubara, Natsumi, Nishiuchi, Tomoko, Zahradnik, Jiri, Andrikopoulos, Prokopios, Padilla-Blanco, Miguel, Konar, Aditi, Fujita, Shigeru, Plianchaisuk, Arnon, Deguchi, Sayaka, Ito, Hayato, Nao, Naganori, Wang, Lei, Nasser, Hesham, Tamura, Tomokazu, Kimura, Izumi, Kashima, Yukie, Suzuki, Rigel, Suzuki, Saori, Kida, Izumi, Tsuda, Masumi, Oda, Yoshitaka, Hashimoto, Rina, Watanabe, Yukio, Uriu, Keiya, Yamasoba, Daichi, Guo, Ziyi, Hinay, Alfredo A., Jr., Kosugi, Yusuke, Chen, Luo, Pan, Lin, Kaku, Yu, Chu, Hin, Donati, Flora, Temmam, Sarah, Eloit, Marc, Yamamoto, Yuki, Nagamoto, Tetsuharu, Asakura, Hiroyuki, Nagashima, Mami, Sadamasu, Kenji, Yoshimura, Kazuhisa, Suzuki, Yutaka, Ito, Jumpei, Ikeda, Terumasa, Tanaka, Shinya, Matsuno, Keita, Fukuhara, Takasuke, Takayama, Kazuo, and Sato, Kei

Published
2024
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3. SARS‐CoV‐2‐related bat virus behavior in human‐relevant models sheds light on the origin of COVID‐19

Author

Temmam, Sarah, Montagutelli, Xavier, Herate, Cécile, Donati, Flora, Regnault, Béatrice, Attia, Mikael, Baquero Salazar, Eduard, Chretien, Delphine, Conquet, Laurine, Jouvion, Grégory, Pipoli Da Fonseca, Juliana, Cokelaer, Thomas, Amara, Faustine, Relouzat, Francis, Naninck, Thibaut, Lemaitre, Julien, Derreudre‐Bosquet, Nathalie, Pascal, Quentin, Bonomi, Massimiliano, Bigot, Thomas, Munier, Sandie, Rey, Felix A, Le Grand, Roger, van der Werf, Sylvie, and Eloit, Marc

Published
2023
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4. Bat coronaviruses related to SARS-CoV-2 and infectious for human cells

Author

Temmam, Sarah, Vongphayloth, Khamsing, Baquero, Eduard, Munier, Sandie, Bonomi, Massimiliano, Regnault, Béatrice, Douangboubpha, Bounsavane, Karami, Yasaman, Chrétien, Delphine, Sanamxay, Daosavanh, Xayaphet, Vilakhan, Paphaphanh, Phetphoumin, Lacoste, Vincent, Somlor, Somphavanh, Lakeomany, Khaithong, Phommavanh, Nothasin, Pérot, Philippe, Dehan, Océane, Amara, Faustine, Donati, Flora, Bigot, Thomas, Nilges, Michael, Rey, Félix A., van der Werf, Sylvie, Brey, Paul T., and Eloit, Marc

Published
2022
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5. Virological characteristics of a SARS-CoV-2-related bat coronavirus, BANAL-20-236

Author

Fujita, Shigeru, primary, Plianchaisuk, Arnon, additional, Deguchi, Sayaka, additional, Ito, Hayato, additional, Nao, Naganori, additional, Wang, Lei, additional, Nasser, Hesham, additional, Tamura, Tomokazu, additional, Kimura, Izumi, additional, Kashima, Yukie, additional, Suzuki, Rigel, additional, Suzuki, Saori, additional, Kida, Izumi, additional, Tsuda, Masumi, additional, Oda, Yoshitaka, additional, Hashimoto, Rina, additional, Watanabe, Yukio, additional, Uriu, Keiya, additional, Yamasoba, Daichi, additional, Guo, Ziyi, additional, Hinay, Alfredo A., additional, Kosugi, Yusuke, additional, Chen, Luo, additional, Pan, Lin, additional, Kaku, Yu, additional, Chu, Hin, additional, Donati, Flora, additional, Temmam, Sarah, additional, Eloit, Marc, additional, Yamamoto, Yuki, additional, Nagamoto, Tetsuharu, additional, Asakura, Hiroyuki, additional, Nagashima, Mami, additional, Sadamasu, Kenji, additional, Yoshimura, Kazuhisa, additional, Suzuki, Yutaka, additional, Sawa, Hirofumi, additional, Mizuma, Keita, additional, Li, Jingshu, additional, Mimura, Yume, additional, Ohari, Yuma, additional, Tsubo, Tomoya, additional, Ferdous, Zannatul, additional, Shishido, Kenji, additional, Mohri, Hiromi, additional, Iida, Miki, additional, Tsujino, Shuhei, additional, Misawa, Naoko, additional, Usui, Kaoru, additional, Saikruang, Wilaiporn, additional, Lytras, Spyridon, additional, Kawakubo, Shusuke, additional, Nishumura, Luca, additional, Mendoza Tolentino, Jarel Elgin, additional, Li, Wenye, additional, Yo, Maximilian Stanley, additional, Horinaka, Kio, additional, Suganami, Mai, additional, Chiba, Mika, additional, Yoshimura, Ryo, additional, Yasuda, Kyoko, additional, Iida, Keiko, additional, Strange, Adam Patrick, additional, Ohsumi, Naomi, additional, Tanaka, Shiho, additional, Ogawa, Eiko, additional, Okumura, Kaho, additional, Fukuda, Tsuki, additional, Osujo, Rina, additional, Yoshida, Isao, additional, Nakagawa, So, additional, Takaori-Kondo, Akifumi, additional, Shirakawa, Kotaro, additional, Nagata, Kayoko, additional, Nomura, Ryosuke, additional, Horisawa, Yoshihito, additional, Tashiro, Yusuke, additional, Kawai, Yugo, additional, Nakata, Yoshitaka, additional, Futatsusako, Hiroki, additional, Sakamoto, Ayaka, additional, Yasuhara, Naoko, additional, Hashiguchi, Takao, additional, Suzuki, Tateki, additional, Kimura, Kanako, additional, Sasaki, Jiei, additional, Nakajima, Yukari, additional, Yajima, Hisano, additional, Irie, Takashi, additional, Kawabata, Ryoko, additional, Sasaki-Tabata, Kaori, additional, Shimizu, Ryo, additional, Monira Begum, M.S.T., additional, Jonathan, Michael, additional, Mugita, Yuka, additional, Leong, Sharee, additional, Takahashi, Otowa, additional, Ichihara, Kimiko, additional, Ueno, Takamasa, additional, Motozono, Chihiro, additional, Toyoda, Mako, additional, Saito, Akatsuki, additional, Kosaka, Anon, additional, Kawano, Miki, additional, Matsubara, Natsumi, additional, Nishiuchi, Tomoko, additional, Zahradnik, Jiri, additional, Andrikopoulos, Prokopios, additional, Padilla-Blanco, Miguel, additional, Konar, Aditi, additional, Ito, Jumpei, additional, Ikeda, Terumasa, additional, Tanaka, Shinya, additional, Matsuno, Keita, additional, Fukuhara, Takasuke, additional, Takayama, Kazuo, additional, and Sato, Kei, additional

Published
2024
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6. Preschool-age children maintain a distinct memory CD4+ T cell and memory B cell response after SARS-CoV-2 infection.

Author

Manfroi, Benoît, Cuc, Bui Thi, Sokal, Aurélien, Vandenberghe, Alexis, Temmam, Sarah, Attia, Mikaël, El Behi, Mohamed, Camaglia, Francesco, Nguyen, Ngan Thu, Pohar, Jelka, Salem-Wehbe, Layale, Pottez-Jouatte, Valentine, Borzakian, Sibyline, Elenga, Narcisse, Galeotti, Caroline, Morelle, Guillaume, de truchis de Lays, Camille, Semeraro, Michaela, Romain, Anne-Sophie, and Aubart, Mélodie

Subjects
SARS-CoV-2, CYTOTOXIC T cells, IMMUNOLOGIC memory, T cells, AGE groups, CORONAVIRUS diseases
Abstract

The development of the human immune system lasts for several years after birth. The impact of this maturation phase on the quality of adaptive immunity and the acquisition of immunological memory after infection at a young age remains incompletely defined. Here, using an antigen-reactive T cell (ARTE) assay and multidimensional flow cytometry, we profiled circulating severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)–reactive CD3+CD4+CD154+ T cells in children and adults before infection, during infection, and 11 months after infection, stratifying children into separate age groups and adults according to disease severity. During SARS-CoV-2 infection, children younger than 5 years old displayed a lower antiviral CD4+ T cell response, whereas children older than 5 years and adults with mild disease had, quantitatively and phenotypically, comparable virus-reactive CD4+ T cell responses. Adults with severe disease mounted a response characterized by higher frequencies of virus-reactive proinflammatory and cytotoxic T cells. After SARS-CoV-2 infection, preschool-age children not only maintained neutralizing SARS-CoV-2–reactive antibodies postinfection comparable to adults but also had phenotypically distinct memory T cells displaying high inflammatory features and properties associated with migration toward inflamed sites. Moreover, preschool-age children had markedly fewer circulating virus-reactive memory B cells compared with the other cohorts. Collectively, our results reveal unique facets of antiviral immunity in humans at a young age and indicate that the maturation of adaptive responses against SARS-CoV-2 toward an adult-like profile occurs in a progressive manner. Editor's summary: The immune system continues to develop throughout childhood, and it is established that children have distinct immune responses to vaccines and infections as compared with adults. Here, Manfroi et al. characterized the immune response to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection in children. The authors found that preschool-age children had a different adaptive immune response, with a lower frequency of antiviral CD4+ T cells, compared with older children and adults; this younger cohort also developed phenotypically distinct memory T and B cell responses after recovery compared with the other cohorts. This study highlights that responses to SARS-CoV-2 infection in young children are unique and reflect the ongoing maturation of the immune system into adulthood. —Courtney Malo [ABSTRACT FROM AUTHOR]

Published
2024
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7. Author Correction: Bat coronaviruses related to SARS-CoV-2 and infectious for human cells

Author

Temmam, Sarah, Vongphayloth, Khamsing, Baquero, Eduard, Munier, Sandie, Bonomi, Massimiliano, Regnault, Béatrice, Douangboubpha, Bounsavane, Karami, Yasaman, Chrétien, Delphine, Sanamxay, Daosavanh, Xayaphet, Vilakhan, Paphaphanh, Phetphoumin, Lacoste, Vincent, Somlor, Somphavanh, Lakeomany, Khaithong, Phommavanh, Nothasin, Pérot, Philippe, Dehan, Océane, Amara, Faustine, Donati, Flora, Bigot, Thomas, Nilges, Michael, Rey, Félix A., van der Werf, Sylvie, Brey, Paul T., and Eloit, Marc

Published
2022
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9. Genotype and Phenotype Characterization of Rhinolophus sp. Sarbecoviruses from Vietnam: Implications for Coronavirus Emergence

Author

Temmam, Sarah, primary, Tu, Tran Cong, additional, Regnault, Béatrice, additional, Bonomi, Massimiliano, additional, Chrétien, Delphine, additional, Vendramini, Léa, additional, Duong, Tran Nhu, additional, Phong, Tran Vu, additional, Yen, Nguyen Thi, additional, Anh, Hoang Ngoc, additional, Son, Tran Hai, additional, Anh, Pham Tuan, additional, Amara, Faustine, additional, Bigot, Thomas, additional, Munier, Sandie, additional, Thong, Vu Dinh, additional, van der Werf, Sylvie, additional, Nam, Vu Sinh, additional, and Eloit, Marc, additional

Published
2023
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10. Comprehensive Characterization of Viral Diversity of Female Mosquitoes in Madagascar

Author

Bennouna, Amal, primary, Tantely, Michael Luciano, additional, Raharinosy, Vololoniaina, additional, Andriamandimby, Soa Fy, additional, Bigot, Thomas, additional, Chrétien, Delphine, additional, Jacquemet, Elise, additional, Volant, Stevenn, additional, Temmam, Sarah, additional, Dussart, Philippe, additional, Lacoste, Vincent, additional, Girod, Romain, additional, and Eloit, Marc, additional

Published
2023
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12. Acute tubulointerstitial nephritis with or without uveitis: a novel form of post-acute COVID-19 syndrome in children

Author

Avramescu, Marina, primary, Isnard, Pierre, additional, Temmam, Sarah, additional, Chevalier, Agnès, additional, Bastard, Paul, additional, Attia, Mikael, additional, Berthaud, Romain, additional, Fila, Marc, additional, Dossier, Claire, additional, Hogan, Julien, additional, Ulinski, Tim, additional, Leguevaques, Damia, additional, Louillet, Férielle, additional, Casado, Edouard Martinez, additional, Halimi, Jean-Michel, additional, Cloarec, Sylvie, additional, Zaloszyc, Ariane, additional, Faudeux, Camille, additional, Rousset-Rouvière, Caroline, additional, Clavé, Stéphanie, additional, Harambat, Jérôme, additional, Rollot, Edouard, additional, Simon, Thomas, additional, Nallet-Amate, Megan, additional, Ranchin, Bruno, additional, Bacchetta, Justine, additional, Porcheret, Florence, additional, Bernard, Josselin, additional, Ryckewaert, Amélie, additional, Jamet, Anne, additional, Fourgeaud, Jacques, additional, Da Rocha, Nicolas, additional, Pérot, Philippe, additional, Kuperwasser, Nicolas, additional, Bouazza, Naïm, additional, Rabant, Marion, additional, Duong Van Huyen, Jean-Paul, additional, Robert, Matthieu P., additional, Zuber, Julien, additional, Casanova, Jean-Laurent, additional, Eloit, Marc, additional, Sermet-Gaudelus, Isabelle, additional, and Boyer, Olivia, additional

Published
2023
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15. De nouveaux coronavirus de chauve-souris similaires à SARS-CoV-2 éclairent l'origine de la pandémie de COVID-19

Author

Temmam, Sarah, Vongphayloth, Khamsing, Baquero Salazar, Eduard, Munier, Sandie, Bonomi, Massimiliano, Regnault, Béatrice, Douangboubpha, Bounsavane, Karami, Yasaman, Chrétien, Delphine, Sanamxay, Daosavanh, Xayaphet, Vilakhan, Paphaphanh, Phetphoumin, Lacoste, Vincent, Somlor, Somphavanh, Lakeomany, Khaithong, Phommavanh, Nothasin, Pérot, Philippe, Dehan, Océane, Amara, Faustine, Donati, Flora, Bigot, Thomas, Nilges, Michael, Rey, Félix, van Der Werf, Sylvie, Brey, Paul, Eloit, Marc, Découverte de pathogènes – Pathogen discovery, Institut Pasteur [Paris] (IP)-Université Paris Cité (UPCité), Centre Collaborateur de l'OIE de Détection et identification chez l’homme des pathogènes animaux émergents et développement d’outils pour leur diagnostic / Collaborating Center for the Detection and identification in humans of emerging animal pathogens and development of tools for their diagnoses (CCOIE-OIECC), Institut Pasteur [Paris] (IP)-Organisation Mondiale de la Santé Animale / World Organisation Animal Health [Paris] (OIE)-Université Paris Cité (UPCité), Institut Pasteur du Laos, Réseau International des Instituts Pasteur (RIIP), Virologie Structurale - Structural Virology, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), Génétique Moléculaire des Virus à ARN - Molecular Genetics of RNA Viruses (GMV-ARN (UMR_3569 / U-Pasteur_2)), Bioinformatique structurale - Structural Bioinformatics, National University of Laos, Centre National de Référence des virus des infections respiratoires (dont la grippe) - National Reference Center Virus Influenzae [Paris] (CNR - laboratoire coordonnateur), Hub Bioinformatique et Biostatistique - Bioinformatics and Biostatistics HUB, and TEMMAM, Sarah

Subjects
[SDV.MP.VIR] Life Sciences [q-bio]/Microbiology and Parasitology/Virology, [SDE.BE] Environmental Sciences/Biodiversity and Ecology, pandémie, [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology, coronavirus, chauve-souris, [SDE.BE]Environmental Sciences/Biodiversity and Ecology, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2022

18. SARS-CoV-2-Related Bat Virus in Human Relevant Models Sheds Light on the Proximal Origin of COVID-19

Author

Temmam, Sarah, primary, Montagutelli, Xavier, additional, Hérate, Cécile, additional, Donati, Flora, additional, Regnault, Beatrice, additional, Attia, Mikaël, additional, Salazar, Eduard Baquero, additional, Chrétien, Delphine, additional, Conquet, Laurine, additional, Jouvion, Grégory, additional, Fonseca, Juliana Pipoli da, additional, Cokelaer, Thomas, additional, Amara, Faustine, additional, Relouzat, Francis, additional, Naninck, Thibaut, additional, Lemaitre, Julien, additional, Dereuddre-Bosquet, Nathalie, additional, Pascal, Quentin, additional, Bonomi, Max, additional, Bigot, Thomas, additional, Munier, Sandie, additional, Rey, Félix, additional, Grand, Roger Le, additional, Werf, Sylvie van der, additional, and Eloit, Marc, additional

Published
2022
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19. MO1039: 1-Year Follow-Up Data of Arterial Abnormalities Identified in Kidneys Transplanted into Children During the First Covid-19 Pandemic Wave

Author

Grapin, Mathilde, primary, Berteloot, Laureline, additional, Berthaud, Romain, additional, Temmam, Sarah, additional, Blanc, Thomas, additional, Charbit, Marina, additional, Pastural, Myriam, additional, Eloit, Marc, additional, Sermet-Gaudelus, Isabelle, additional, Dehoux, Laurène, additional, and Boyer, Olivia, additional

Published
2022
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21. The virome of Rhipicephalus, Dermacentor and Haemaphysalis ticks from Eastern Romania includes novel viruses with potential relevance for public health.

Author

Bratuleanu, Bianca Elena, Temmam, Sarah, Chrétien, Delphine, Regnault, Béatrice, Pérot, Philippe, Bouchier, Christiane, Bigot, Thomas, Savuţa, Gheorghe, and Eloit, Marc

Subjects
*TICKS, *DERMACENTOR, *RHIPICEPHALUS, *TICK-borne diseases, *BIRD migration, *VIRUS diversity
Abstract

Ticks are involved in the transmission of various pathogens and several tick-borne diseases cause significant problems for the health of humans and livestock. The composition of viral communities in ticks and their interactions with pathogens, is poorly understood, particularly in Eastern Europe, an area that represents a major hub for animal-arthropod vectors exchanges (e.g., via bird migrations). The aim of this study was to describe the virome of Dermacentor sp., Rhipicephalus sp. and Haemaphysalis sp. ticks collected from relatively little studied regions of Romania (Iasi and Tulcea counties) located at the intersection of various biotopes, countries and routes of migrations. We also focused the study on viruses that could potentially have relevance for human and animal health. In 2019, more than 500 ticks were collected from the vegetation and from small ruminants and analysed by high-throughput transcriptome sequencing. Among the viral communities infecting Romanian ticks, viruses belonging to the Flaviviridae, Phenuiviridae and Nairoviridae families were identified and full genomes were derived. Phylogenetic analyses placed them in clades where mammalian isolates are found, suggesting that these viruses could constitute novel arboviruses. The characterization of these communities increase the knowledge of the diversity of viruses in Eastern Europe and provides a basis for further studies about the interrelationship between ticks and tick-borne viruses. [ABSTRACT FROM AUTHOR]

Published
2022
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22. Preschool-age children maintain a distinct memory CD4+T cell and memory B cell response after SARS-CoV-2 infection

Author

Manfroi, Benoît, Cuc, Bui Thi, Sokal, Aurélien, Vandenberghe, Alexis, Temmam, Sarah, Attia, Mikaël, El Behi, Mohamed, Camaglia, Francesco, Nguyen, Ngan Thu, Pohar, Jelka, Salem-Wehbe, Layale, Pottez-Jouatte, Valentine, Borzakian, Sibyline, Elenga, Narcisse, Galeotti, Caroline, Morelle, Guillaume, de truchis de Lays, Camille, Semeraro, Michaela, Romain, Anne-Sophie, Aubart, Mélodie, Ouldali, Naim, Mahuteau-Betzer, Florence, Beauvineau, Claire, Amouyal, Elsa, Berthaud, Romain, Crétolle, Célia, Arnould, Marc Duval, Faye, Albert, Lorrot, Mathie, Benoist, Grégoire, Briand, Nelly, Courbebaisse, Marie, Martin, Roland, Van Endert, Peter, Hulot, Jean-Sébastien, Blanchard, Anne, Tartour, Eric, Leite-de-Moraes, Maria, Lezmi, Guillaume, Ménager, Mickael, Luka, Marine, Reynaud, Claude-Agnès, Weill, Jean-Claude, Languille, Laetitia, Michel, Marc, Chappert, Pascal, Mora, Thierry, Walczak, Aleksandra M., Eloit, Marc, Bacher, Petra, Scheffold, Alexander, Mahévas, Matthieu, Sermet-Gaudelus, Isabelle, and Fillatreau, Simon

Abstract

The development of the human immune system lasts for several years after birth. The impact of this maturation phase on the quality of adaptive immunity and the acquisition of immunological memory after infection at a young age remains incompletely defined. Here, using an antigen-reactive T cell (ARTE) assay and multidimensional flow cytometry, we profiled circulating severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)–reactive CD3+CD4+CD154+T cells in children and adults before infection, during infection, and 11 months after infection, stratifying children into separate age groups and adults according to disease severity. During SARS-CoV-2 infection, children younger than 5 years old displayed a lower antiviral CD4+T cell response, whereas children older than 5 years and adults with mild disease had, quantitatively and phenotypically, comparable virus-reactive CD4+T cell responses. Adults with severe disease mounted a response characterized by higher frequencies of virus-reactive proinflammatory and cytotoxic T cells. After SARS-CoV-2 infection, preschool-age children not only maintained neutralizing SARS-CoV-2–reactive antibodies postinfection comparable to adults but also had phenotypically distinct memory T cells displaying high inflammatory features and properties associated with migration toward inflamed sites. Moreover, preschool-age children had markedly fewer circulating virus-reactive memory B cells compared with the other cohorts. Collectively, our results reveal unique facets of antiviral immunity in humans at a young age and indicate that the maturation of adaptive responses against SARS-CoV-2 toward an adult-like profile occurs in a progressive manner.

Published
2024
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23. Preschool-age children maintain a distinct memory CD4 + T cell and memory B cell response after SARS-CoV-2 infection.

Author

Manfroi B, Cuc BT, Sokal A, Vandenberghe A, Temmam S, Attia M, El Behi M, Camaglia F, Nguyen NT, Pohar J, Salem-Wehbe L, Pottez-Jouatte V, Borzakian S, Elenga N, Galeotti C, Morelle G, de Truchis de Lays C, Semeraro M, Romain AS, Aubart M, Ouldali N, Mahuteau-Betzer F, Beauvineau C, Amouyal E, Berthaud R, Crétolle C, Arnould MD, Faye A, Lorrot M, Benoist G, Briand N, Courbebaisse M, Martin R, Van Endert P, Hulot JS, Blanchard A, Tartour E, Leite-de-Moraes M, Lezmi G, Ménager M, Luka M, Reynaud CA, Weill JC, Languille L, Michel M, Chappert P, Mora T, Walczak AM, Eloit M, Bacher P, Scheffold A, Mahévas M, Sermet-Gaudelus I, and Fillatreau S

Subjects
Humans, Child, Preschool, Adult, Child, Memory T Cells immunology, Male, Immunologic Memory, Female, Antibodies, Viral immunology, Antibodies, Viral blood, Middle Aged, Antibodies, Neutralizing immunology, Antibodies, Neutralizing blood, Young Adult, COVID-19 immunology, COVID-19 virology, SARS-CoV-2 immunology, CD4-Positive T-Lymphocytes immunology, Memory B Cells immunology
Abstract

The development of the human immune system lasts for several years after birth. The impact of this maturation phase on the quality of adaptive immunity and the acquisition of immunological memory after infection at a young age remains incompletely defined. Here, using an antigen-reactive T cell (ARTE) assay and multidimensional flow cytometry, we profiled circulating severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-reactive CD3 + CD4 + CD154 + T cells in children and adults before infection, during infection, and 11 months after infection, stratifying children into separate age groups and adults according to disease severity. During SARS-CoV-2 infection, children younger than 5 years old displayed a lower antiviral CD4 + T cell response, whereas children older than 5 years and adults with mild disease had, quantitatively and phenotypically, comparable virus-reactive CD4 + T cell responses. Adults with severe disease mounted a response characterized by higher frequencies of virus-reactive proinflammatory and cytotoxic T cells. After SARS-CoV-2 infection, preschool-age children not only maintained neutralizing SARS-CoV-2-reactive antibodies postinfection comparable to adults but also had phenotypically distinct memory T cells displaying high inflammatory features and properties associated with migration toward inflamed sites. Moreover, preschool-age children had markedly fewer circulating virus-reactive memory B cells compared with the other cohorts. Collectively, our results reveal unique facets of antiviral immunity in humans at a young age and indicate that the maturation of adaptive responses against SARS-CoV-2 toward an adult-like profile occurs in a progressive manner.

Published
2024
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24. Seroprevalence of Crimean-Congo Hemorrhagic Fever Among Small Ruminants from Southern Romania.

Author

Bratuleanu B, Anita A, Temmam S, Dascalu A, Crivei L, Cozma A, Pourquier P, Savuta G, Eloit M, and Anita D

Subjects
Animals, Antibodies, Viral, Humans, Romania epidemiology, Ruminants, Seroepidemiologic Studies, Sheep, Goat Diseases epidemiology, Hemorrhagic Fever Virus, Crimean-Congo genetics, Hemorrhagic Fever, Crimean epidemiology, Hemorrhagic Fever, Crimean veterinary, Sheep Diseases epidemiology, Ticks
Abstract

Crimean-Congo hemorrhagic fever (CCHF) is a tick-borne disease that can be contracted by direct contact with viremic animals or humans. Domestic animals are accidental hosts and contribute to the spread and amplification of the virus. The main objective of this study was to provide updated information related to CCHF virus (CCHFV) infection in Southern Romania by assessing the seroprevalence of CCHF in small ruminants (sheep and goats) using a double-antigen sandwich enzyme-linked immunosorbent assay and by detection of CCHFV in engorged ticks and serum samples using real-time RT-PCR. The overall seroprevalence of CCHF in small ruminants was 37.7% (95% CI 31.7 to 43.7). No statistical seroprevalence difference was observed between the two species of ruminants ( p  = 0.76), but a significant difference was established between the locations ( p  < 0.01). No CCHFV RNA was detected in tick pools and small ruminant's sera tested by real-time RT-PCR, although the high seroprevalence to CCHFV among ruminants indicates that CCHV or a closely related virus circulates in Southern Romania.

Published
2022
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