Search

Your search keyword '"Vera L. Lanchote"' showing total 2 results
Start Over Author "Vera L. Lanchote" Publication Year Range Last 3 years
2 results on '"Vera L. Lanchote"'

1. Potential cannabidiol (CBD) repurposing as antibacterial and promising therapy of CBD plus polymyxin B (PB) against PB-resistant gram-negative bacilli

Author

Nathália Abichabki, Luísa V. Zacharias, Natália C. Moreira, Fernando Bellissimo-Rodrigues, Fernanda L. Moreira, Jhohann R. L. Benzi, Tânia M. C. Ogasawara, Joseane C. Ferreira, Camila M. Ribeiro, Fernando R. Pavan, Leonardo R. L. Pereira, Guilherme T. P. Brancini, Gilberto Ú. L. Braga, Antonio W. Zuardi, Jaime E. C. Hallak, José A. S. Crippa, Vera L. Lanchote, Rafael Cantón, Ana Lúcia C. Darini, and Leonardo N. Andrade

Subjects
Medicine, Science
Abstract

Abstract This study aimed to assess the ultrapure cannabidiol (CBD) antibacterial activity and to investigate the antibacterial activity of the combination CBD + polymyxin B (PB) against Gram-negative (GN) bacteria, including PB-resistant Gram-negative bacilli (GNB). We used the standard broth microdilution method, checkerboard assay, and time-kill assay. CBD exhibited antibacterial activity against Gram-positive bacteria, lipooligosaccharide (LOS)-expressing GN diplococcus (GND) (Neisseria gonorrhoeae, Neisseria meningitidis, Moraxella catarrhalis), and Mycobacterium tuberculosis, but not against GNB. For most of the GNB studied, our results showed that low concentrations of PB (≤ 2 µg/mL) allow CBD (≤ 4 µg/mL) to exert antibacterial activity against GNB (e.g., Klebsiella pneumoniae, Escherichia coli, Acinetobacter baumannii), including PB-resistant GNB. CBD + PB also showed additive and/or synergistic effect against LOS-expressing GND. Time-kill assays results showed that the combination CBD + PB leads to a greater reduction in the number of colony forming units per milliliter compared to CBD and PB alone, at the same concentration used in combination, and the combination CBD + PB was synergistic for all four PB-resistant K. pneumoniae isolates evaluated. Our results show that CBD has translational potential and should be further explored as a repurposed antibacterial agent in clinical trials. The antibacterial efficacy of the combination CBD + PB against multidrug-resistant and extensively drug-resistant GNB, especially PB-resistant K. pneumoniae, is particularly promising.

Published
2022
Full Text
View/download PDF

2. Potential cannabidiol (CBD) repurposing as antibacterial and promising therapy of CBD plus polymyxin B (PB) against PB-resistant gram-negative bacilli

Author

Nathália, Abichabki, Luísa V, Zacharias, Natália C, Moreira, Fernando, Bellissimo-Rodrigues, Fernanda L, Moreira, Jhohann R L, Benzi, Tânia M C, Ogasawara, Joseane C, Ferreira, Camila M, Ribeiro, Fernando R, Pavan, Leonardo R L, Pereira, Guilherme T P, Brancini, Gilberto Ú L, Braga, Antonio W, Zuardi, Jaime E C, Hallak, José A S, Crippa, Vera L, Lanchote, Rafael, Cantón, Ana Lúcia C, Darini, and Leonardo N, Andrade

Subjects
Klebsiella pneumoniae, Drug Resistance, Multiple, Bacterial, Gram-Negative Bacteria, Drug Repositioning, Cannabidiol, Drug Synergism, Microbial Sensitivity Tests, Anti-Bacterial Agents, Polymyxin B
Abstract

This study aimed to assess the ultrapure cannabidiol (CBD) antibacterial activity and to investigate the antibacterial activity of the combination CBD + polymyxin B (PB) against Gram-negative (GN) bacteria, including PB-resistant Gram-negative bacilli (GNB). We used the standard broth microdilution method, checkerboard assay, and time-kill assay. CBD exhibited antibacterial activity against Gram-positive bacteria, lipooligosaccharide (LOS)-expressing GN diplococcus (GND) (Neisseria gonorrhoeae, Neisseria meningitidis, Moraxella catarrhalis), and Mycobacterium tuberculosis, but not against GNB. For most of the GNB studied, our results showed that low concentrations of PB (≤ 2 µg/mL) allow CBD (≤ 4 µg/mL) to exert antibacterial activity against GNB (e.g., Klebsiella pneumoniae, Escherichia coli, Acinetobacter baumannii), including PB-resistant GNB. CBD + PB also showed additive and/or synergistic effect against LOS-expressing GND. Time-kill assays results showed that the combination CBD + PB leads to a greater reduction in the number of colony forming units per milliliter compared to CBD and PB alone, at the same concentration used in combination, and the combination CBD + PB was synergistic for all four PB-resistant K. pneumoniae isolates evaluated. Our results show that CBD has translational potential and should be further explored as a repurposed antibacterial agent in clinical trials. The antibacterial efficacy of the combination CBD + PB against multidrug-resistant and extensively drug-resistant GNB, especially PB-resistant K. pneumoniae, is particularly promising.

Published
2021

Catalog

Books, media, physical & digital resources
See catalog results