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Your search keyword '"Węglarz‐tomczak, E"' showing total 2 results
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2 results on '"Węglarz‐tomczak, E"'

1. Fusion–fission–mitophagy cycling and metabolic reprogramming coordinate nerve growth factor (NGF)‐dependent neuronal differentiation

Author

Goglia, I, Węglarz‐tomczak, E, Gioia, C, Liu, Y, Virtuoso, A, Bonanomi, M, Gaglio, D, Salmistraro, N, De Luca, C, Papa, M, Alberghina, L, Westerhoff, H, Colangelo, A, Goglia, Ilaria, Węglarz‐Tomczak, Ewelina, Gioia, Claudio, Liu, Yanhua, Virtuoso, Assunta, Bonanomi, Marcella, Gaglio, Daniela, Salmistraro, Noemi, De Luca, Ciro, Papa, Michele, Alberghina, Lilia, Westerhoff, Hans V., Colangelo, Anna Maria, Goglia, I, Węglarz‐tomczak, E, Gioia, C, Liu, Y, Virtuoso, A, Bonanomi, M, Gaglio, D, Salmistraro, N, De Luca, C, Papa, M, Alberghina, L, Westerhoff, H, Colangelo, A, Goglia, Ilaria, Węglarz‐Tomczak, Ewelina, Gioia, Claudio, Liu, Yanhua, Virtuoso, Assunta, Bonanomi, Marcella, Gaglio, Daniela, Salmistraro, Noemi, De Luca, Ciro, Papa, Michele, Alberghina, Lilia, Westerhoff, Hans V., and Colangelo, Anna Maria

Abstract

Neuronal differentiation is regulated by nerve growth factor (NGF) and other neurotrophins. We explored the impact of NGF on mitochondrial dynamics and metabolism through time-lapse imaging, metabolomics profiling, and computer modeling studies. We show that NGF may direct differentiation by stimulating fission, thereby causing selective mitochondrial network fragmentation and mitophagy, ultimately leading to increased mitochondrial quality and respiration. Then, we reconstructed the dynamic fusion–fission–mitophagy cycling of mitochondria in a computer model, integrating these processes into a single network mechanism. Both the computational model and the simulations are able to reproduce the proposed mechanism in terms of mitochondrial dynamics, levels of reactive oxygen species (ROS), mitophagy, and mitochondrial quality, thus providing a computational tool for the interpretation of the experimental data and for future studies aiming to detail further the action of NGF on mitochondrial processes. We also show that changes in these mitochondrial processes are intertwined with a metabolic function of NGF in differentiation: NGF directs a profound metabolic rearrangement involving glycolysis, TCA cycle, and the pentose phosphate pathway, altering the redox balance. This metabolic rewiring may ensure: (a) supply of both energy and building blocks for the anabolic processes needed for morphological reorganization, as well as (b) redox homeostasis.

Published
2024

2. Fusion-fission-mitophagy cycling and metabolic reprogramming coordinate nerve growth factor (NGF)-dependent neuronal differentiation.

Author

Goglia I, Węglarz-Tomczak E, Gioia C, Liu Y, Virtuoso A, Bonanomi M, Gaglio D, Salmistraro N, De Luca C, Papa M, Alberghina L, Westerhoff HV, and Colangelo AM

Subjects
Animals, PC12 Cells, Rats, Citric Acid Cycle drug effects, Glycolysis, Computer Simulation, Metabolic Reprogramming, Nerve Growth Factor metabolism, Nerve Growth Factor pharmacology, Nerve Growth Factor genetics, Mitochondrial Dynamics drug effects, Cell Differentiation, Neurons metabolism, Neurons cytology, Neurons drug effects, Mitochondria metabolism, Reactive Oxygen Species metabolism, Mitophagy drug effects
Abstract

Neuronal differentiation is regulated by nerve growth factor (NGF) and other neurotrophins. We explored the impact of NGF on mitochondrial dynamics and metabolism through time-lapse imaging, metabolomics profiling, and computer modeling studies. We show that NGF may direct differentiation by stimulating fission, thereby causing selective mitochondrial network fragmentation and mitophagy, ultimately leading to increased mitochondrial quality and respiration. Then, we reconstructed the dynamic fusion-fission-mitophagy cycling of mitochondria in a computer model, integrating these processes into a single network mechanism. Both the computational model and the simulations are able to reproduce the proposed mechanism in terms of mitochondrial dynamics, levels of reactive oxygen species (ROS), mitophagy, and mitochondrial quality, thus providing a computational tool for the interpretation of the experimental data and for future studies aiming to detail further the action of NGF on mitochondrial processes. We also show that changes in these mitochondrial processes are intertwined with a metabolic function of NGF in differentiation: NGF directs a profound metabolic rearrangement involving glycolysis, TCA cycle, and the pentose phosphate pathway, altering the redox balance. This metabolic rewiring may ensure: (a) supply of both energy and building blocks for the anabolic processes needed for morphological reorganization, as well as (b) redox homeostasis., (© 2024 The Authors. The FEBS Journal published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies.)

Published
2024
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