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580 results on '"Woodworth, Jason C."'

3. Diagnostic survey of analytical methods used to determine bone mineralization in pigs

Author

Williams, Hadley R, primary, Tokach, Mike D, additional, Woodworth, Jason C, additional, DeRouchey, Joel M, additional, Goodband, Robert D, additional, Bergstrom, Jon R, additional, Rahe, Michael C, additional, Siepker, Christopher L, additional, Sitthicharoenchai, Panchan, additional, Ensley, Steve M, additional, Radke, Scott L, additional, and Gebhardt, Jordan T, additional

Published
2024
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4. Development and optimization of sampling techniques for environmental samples from African swine fever virus-contaminated surfaces with no organic contaminants.

Author

Taeyong Kwon, Gebhardt, Jordan T., Eu Lim Lyoo, Gaudreault, Natasha N., Trujillo, Jessie D., Woodworth, Jason C., Paulk, Chad B., Jones, Cassandra K., and Richt, Juergen A.

Subjects
AFRICAN swine fever, ENVIRONMENTAL sampling, SURGICAL sponges, WILD boar, COMMUNICABLE diseases
Abstract

African swine fever (ASF) is a highly contagious diseases in domestic pigs and wild boars with up to 100% mortality. ASF virus (ASFV) is a causative agent responsible for ASF and highly resistant in environments, which creates a significant challenge for the control and eradication of the virus. Despite the geographical expansion of ASFV and internationalmovement of products to sustain the swine production system, there is limited knowledge on the use of environmental samples to perform surveillance to prevent the introduction of ASFV into ASFV-free areas and for control of transmission in affected areas. Therefore, this study aimed to develop and optimize sampling techniques for environmental samples for ASFV detection. The stainless steel surfaces were contaminated with ASFV-infected blood, swabbed using different devices, and then processed through different techniques. The environmental samples were processed and tested using qPCR analysis. The results showed that the use of pre-moistened gauze surgical sponges, sweeping pads, and sponge sticks resulted in increased sensitivity, when compared to either dry sampling devices or Dacron swab. In particular, the combination of the sponge stick and the commercial nucleic acid preservative supported the best detection of ASFV DNA on the clean stainless steel surfaces evaluated. Pre-incubation for the short period of time and centrifugation at low speed were sufficient to provide satisfactory diagnostic sensitivity of ASFV detection using qPCR for environmental samples. Our findings contribute to the development of techniques for environmental samples for ASFV surveillance to prevent the introduction and dissemination of ASFV. [ABSTRACT FROM AUTHOR]

Published
2024
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5. Skeletal Muscle Metabolism Is Dynamic during Porcine Postnatal Growth.

Author

Rimmer, Linnea A., Geisbrecht, Erika R., Chao, Michael D., O'Quinn, Travis G., Woodworth, Jason C., and Zumbaugh, Morgan D.

Subjects
MUSCLE metabolism, METABOLOMIC fingerprinting, SKELETAL muscle, SWINE nutrition, PYRUVATE carboxylase, LATISSIMUS dorsi (Muscles)
Abstract

Skeletal muscle metabolism has implications for swine feed efficiency (FE); however, it remains unclear if the metabolic profile of skeletal muscle changes during postnatal growth. To assess the metabolic changes, samples were collected from the longissimus dorsi (LD, glycolytic muscle), latissimus dorsi (LAT, mixed muscle), and masseter (MS, oxidative muscle) at 20, 53, 87, 120, and 180 days of age from barrows. Muscles were assessed to determine the abundance of several metabolic enzymes. Lactate dehydrogenase (LDHα) decreased in all muscles from 20 to 87 d (p < 0.01), which may be attributed to the muscles being more glycolytic at weaning from a milk-based diet. Pyruvate carboxylase (PC) increased in all muscles at 53 d compared to the other time points (p < 0.01), while pyruvate dehydrogenase α 1 (PDHα1) increased at 87 and 180 d in MS compared to LD (p < 0.05), indicating that potential changes occur in pyruvate entry into the tricarboxylic acid (TCA) cycle during growth. Isolated mitochondria from each muscle were incubated with 13C-labeled metabolites to assess isotopomer enrichment patterns of TCA intermediates. Citrate M + 2 and M + 4 derived from [13C3]-pyruvate increased at 87 d in LAT and MS mitochondria compared to LD mitochondria (p < 0.05). Regardless of the muscle, citrate M+3 increased at 87 d compared to 20, 53, and 120 d, while 180 d showed intermediate values (p < 0.01). These data support the notion that pyruvate metabolism is dynamic during growth. Our findings establish a metabolic fingerprint associated with postnatal muscle hypertrophy. [ABSTRACT FROM AUTHOR]

Published
2024
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6. Evaluation of Truck Cab Decontamination Procedures following Inoculation with Porcine Epidemic Diarrhea Virus and Porcine Reproductive and Respiratory Syndrome Virus

Author

Houston, Grace E., primary, Jones, Cassandra K., additional, Evans, Caitlin, additional, Otott, Haley K., additional, Stark, Charles R., additional, Bai, Jianfa, additional, Poulsen Porter, Elizabeth G., additional, de Almeida, Marcelo N., additional, Zhang, Jianqiang, additional, Gauger, Phillip C., additional, Blomme, Allison K., additional, Woodworth, Jason C., additional, Paulk, Chad B., additional, and Gebhardt, Jordan T., additional

Published
2024
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9. Effect of the pelleting process on diet formulations with varying levels of crystalline amino acids and reducing sugars on digestibility in growing pigs

Author

Dunmire, Kara M, primary, Lopez, Diego A, additional, Zhang, Yiqin, additional, Jones, Cassandra K, additional, Li, Yonghui, additional, Woodworth, Jason C, additional, Goodband, Robert D, additional, Tokach, Michael D, additional, Stark, Charles R, additional, Stein, Hans H, additional, and Paulk, Chad B, additional

Published
2024
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10. Detection of African Swine Fever Virus in Feed and Feed Mill Environment Following Extended Storage

Author

National Pork Board (US), Center of Excellence for Emerging and Zoonotic Animal Diseases (US), Houston, Grace E. [0000-0001-8113-1616], Trujillo, Jessie D. [0000-0002-4930-5839], Cool, Konner R. [0000-0003-3666-0214], Paulk, Chad B. [0000-0001-8844-6477], Gaudreault, Natasha N. [0000-0002-7195-785X], Woodworth, Jason C. [0000-0001-7268-4278], Morozov, Igor [0000-0002-9343-5446], Gallardo, Carmina [0000-0003-3293-306X], Gebhardt, Jordan T. [0000-0002-6144-6714], Richt, Jürgen A. [0000-0001-7308-5672], Houston, Grace E., Trujillo, Jessie D., Jones, Cassandra K., Kwon, Taeyong, Stark, Charles R., Cool, Konner R., Paulk, Chad B., Gaudreault, Natasha N., Woodworth, Jason C., Morozov, Igor, Gallardo, Carmina, Gebhardt, Jordan T., Richt, Jürgen A., National Pork Board (US), Center of Excellence for Emerging and Zoonotic Animal Diseases (US), Houston, Grace E. [0000-0001-8113-1616], Trujillo, Jessie D. [0000-0002-4930-5839], Cool, Konner R. [0000-0003-3666-0214], Paulk, Chad B. [0000-0001-8844-6477], Gaudreault, Natasha N. [0000-0002-7195-785X], Woodworth, Jason C. [0000-0001-7268-4278], Morozov, Igor [0000-0002-9343-5446], Gallardo, Carmina [0000-0003-3293-306X], Gebhardt, Jordan T. [0000-0002-6144-6714], Richt, Jürgen A. [0000-0001-7308-5672], Houston, Grace E., Trujillo, Jessie D., Jones, Cassandra K., Kwon, Taeyong, Stark, Charles R., Cool, Konner R., Paulk, Chad B., Gaudreault, Natasha N., Woodworth, Jason C., Morozov, Igor, Gallardo, Carmina, Gebhardt, Jordan T., and Richt, Jürgen A.

Abstract

One way to mitigate risk of feed-based pathogens for swine diets is to quarantine feed ingredients before inclusion in complete diets. Data have been generated evaluating the stability of swine viruses in ingredients, but the stability of African swine fever virus (ASFV) in feed or in a feed manufacturing environment has not been well characterized. Therefore, this study aimed to determine the stability of ASFV DNA in swine feed and on mill surfaces over time. A pilot-scale feed mill was used to manufacture six sequential batches of feed consisting of a batch of ASFV-free feed, followed by a batch inoculated with ASFV (final concentration = 5.6 × 104 TCID50/g), and then four subsequent ASFV-free batches. After each batch, 10 feed samples were aseptically collected in a double “X” pattern. During feed manufacturing, 24 steel coupons were placed on the floor of the manufacturing area and allowed to collect dust during feed manufacturing. Once feed manufacturing was completed, feed samples and steel coupons were stored at room temperature. Three of each were randomly selected from storage on 3, 7, 14, 28, 60, 90, and 180 days after feed manufacturing and analyzed for ASFV DNA. For feed samples, there was evidence of a batch × day interaction (P ¼ 0:023) for the quantification of genomic copies/g of feed, indicating that the amount of ASFV DNA present was impacted by both the batch of feed and days held at room temperature. There were no differences of genomic copies/g in early batches, but quantity of detectable ASFV decreased with increasing storage time. In Batches 4–6, the greatest quantity of ASFV DNA was detected on the day of feed manufacturing. The lowest quantity was detected on Day 7 for Batch 4, Day 60 for Batch 5, and at 28 and 180 days for Batch 6. There was no evidence of ASFV degradation on environmental discs across holding times (P ¼ 0:433). In conclusion, the quarantining of feed may help reduce but not eliminate the presence of ASFV DNA in feed over t

Published
2023

13. 245 Effects of Standardized Ileal Digestible Tryptophan to Lysine Ratio on Growth Performance of PIC Line 337-Sired Grow-Finish Pigs

Author

Royall, Rafe Q, primary, Tokach, Mike D, additional, Woodworth, Jason C, additional, DeRouchey, Joel M, additional, Goodband, Robert D, additional, Gebhardt, Jordan T, additional, Vier, Carine M, additional, Spindler, Matthew, additional, Orlando, Uislei A, additional, Zaragoza, Luis E, additional, Lu, Ning, additional, Cast, Wayne R, additional, Wilson-Wells, Danielle, additional, Holen, Julia, additional, and Betlach, Alyssa, additional

Published
2023
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18. 46 The Distribution of Porcine Epidemic Diarrhea Virus, Porcine Reproductive and Respiratory Syndrome Virus, and Seneca Valley Virus 1 Inoculated Feed After Chemical Mitigation to Flush a Feed Mill

Author

Harrison, Olivia, primary, Otott, Haley, additional, Bai, Jianfa, additional, Hamill, Vaughn, additional, Woodworth, Jason C, additional, Jones, Cassandra K, additional, Stark, Charles R, additional, Pogranichniy, Roman, additional, Gebhardt, Jordan T, additional, and Paulk, Chad B, additional

Published
2023
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25. 129 Effects of Standardized Ileal Digestible Lysine on Growth Performance of 12- to 136-kg PIC Line 800-Sired Pigs

Author

Gaffield, Katelyn, primary, Tokach, Mike D, additional, Woodworth, Jason C, additional, DeRouchey, Joel M, additional, Goodband, Robert D, additional, Gebhardt, Jordan T, additional, Vier, Carine M, additional, Spindler, Matthew, additional, Orlando, Uislei A, additional, Zaragoza, Luis E, additional, Lu, Ning, additional, Cast, Wayne R, additional, Wilson-Wells, Danielle, additional, Holen, Julia, additional, and Betlach, Alyssa, additional

Published
2023
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42. 240 Comparing Increasing Tryptophan: Lysine Ratios in Distiller Dried Grains-Based Diets with or without Distiller Dried Grains withdrawal Strategy on Growth and Carcass Performance of Grow-Finish Pigs

Author

Spinler, Mikayla, primary, Tolosa, Andres F, additional, Woodworth, Jason C, additional, Tokach, Mike D, additional, Goodband, Robert D, additional, DeRouchey, Joel M, additional, Coble, Kyle, additional, Gerhart, Amanda J, additional, Soto, Jose A, additional, and Gebhardt, Jordan T, additional

Published
2023
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44. The effect of bone and analytical methods on the assessment of bone mineralization response to dietary phosphorus, phytase, and vitamin D in nursery pigs

Author

Williams, Hadley R, primary, Chin, Taylor E, additional, Tokach, Mike D, additional, Woodworth, Jason C, additional, DeRouchey, Joel M, additional, Goodband, Robert D, additional, Bergstrom, Jon R, additional, Rahe, Michael C, additional, Siepker, Christopher L, additional, Sitthicharoenchai, Panchan, additional, Radke, Scott L, additional, Ensley, Steve M, additional, and Gebhardt, Jordan T, additional

Published
2023
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46. Detection of African Swine Fever Virus in Feed and Feed Mill Environment Following Extended Storage

Author

Houston, Grace E., primary, Trujillo, Jessie D., additional, Jones, Cassandra K., additional, Kwon, Taeyong, additional, Stark, Charles R., additional, Cool, Konner, additional, Paulk, Chad B., additional, Gaudreault, Natasha N., additional, Woodworth, Jason C., additional, Morozov, Igor, additional, Gallardo, Carmina, additional, Gebhardt, Jordan T., additional, and Richt, Jürgen A., additional

Published
2023
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47. Comparing tryptophan:lysine ratios in dried distillers grains with solubles-based diets with and without a dried distillers grains with solubles withdrawal strategy on growth, carcass characteristics, and carcass fat iodine value of growing-finishing pigs

Author

Spinler, Mikayla S, primary, Tolosa, Andres F, additional, Gebhardt, Jordan T, additional, Tokach, Mike D, additional, Goodband, Robert D, additional, DeRouchey, Joel M, additional, Coble, Kyle F, additional, and Woodworth, Jason C, additional

Published
2023
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