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Your search keyword '"Wu, Chu-Tse"' showing total 19 results
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19 results on '"Wu, Chu-Tse"'

13. The Antithrombotic Effect of Recombinant Neorudin on Thrombi

Author

Liu, Yu-Bin, primary, Zhang, Lin, additional, Zhou, Xing-Chen, additional, Zhou, Ying, additional, Liu, Yun, additional, Zheng, Can, additional, Xu, Xiao, additional, Geng, Pan, additional, Hao, Chun-Hua, additional, Zhao, Zhuan-You, additional, Wu, Chu-Tse, additional, and Jin, Ji-De, additional

Published
2022
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17. Dental Pulp Mesenchymal Stem Cells Attenuate Limb Ischemia via Promoting Capillary Proliferation and Collateral Development in a Preclinical Model

Author

Li, Youfeng, primary, Zhang, Yuning, additional, Wang, Hua, additional, Sun, Chengfeng, additional, Liu, Dongmei, additional, Liu, Hongying, additional, He, Jia, additional, Chen, Furong, additional, Wang, Weiting, additional, Jiang, Xi, additional, Wu, Chu-tse, additional, and Yang, Yuefeng, additional

Published
2021
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18. Microgel-based carriers enhance skeletal stem cell reprogramming towards immunomodulatory phenotype in osteoarthritic therapy

Author

Li, Pei-Lin, Chen, Da-Fu, Li, Xiao-Tong, Hao, Rui-Cong, Zhao, Zhi-Dong, Li, Zhi-Ling, Yin, Bo-Feng, Tang, Jie, Luo, Yu-Wen, Wu, Chu-Tse, Nie, Jing-Jun, and Zhu, Heng

Abstract

Skeletal stem cells (SSC) have gained attentions as candidates for the treatment of osteoarthritis due to their osteochondrogenic capacity. However, the immunomodulatory properties of SSC, especially under delivery operations, have been largely ignored. In the study, we found that Pdpn+and Grem1+SSC subpopulations owned immunoregulatory potential, and the single-cell RNA sequencing (scRNA-seq) data suggested that the mechanical activation of microgel carriers on SSC induced the generation of Pdpn+Grem1+Ptgs2+SSC subpopulation, which was potent at suppressing macrophage inflammation. The microgel carriers promoted the YAP nuclear translocation, and the activated YAP protein was necessary for the increased expression of Ptgs2 and PGE2in microgels-delivered SSC, which further suppressed the expression of TNF-ɑ, IL-1β and promoted the expression of IL-10 in macrophages. SSC delivered with microgels yielded better preventive effects on articular lesions and macrophage activation in osteoarthritic rats than SSC without microgels. Chemically blocking the YAP and Ptgs2 in microgels-delivered SSC partially abolished the enhanced protection on articular tissues and suppression on osteoarthritic macrophages. Moreover, microgel carriers significantly prolonged SSC retention time in vivowithout increasing SSC implanting into osteoarthritic joints. Together, our study demonstrated that microgel carriers enhanced SSC reprogramming towards immunomodulatory phenotype to regulate macrophage phenotype transformation for effectively osteoarthritic therapy by promoting YAP protein translocation into nucleus. The study not only complement and perfect the immunological mechanisms of SSC-based therapy at the single-cell level, but also provide new insight for microgel carriers in stem cell-based therapy.

Published
2023
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19. The toxicity assessment of neorudin in cynomolgus monkeys.

Author

Liu YB, Ren BY, Zhou XC, Zhang L, Liu Y, Zheng C, Wei JF, Wu CT, and Jin JD

Subjects
Animals, Dose-Response Relationship, Drug, Infusions, Intravenous, Injections, Intravenous, Macaca fascicularis, Saline Solution administration & dosage, Cardiovascular System drug effects, Hirudins administration & dosage, Hirudins toxicity, Respiratory System drug effects, Toxicity Tests, Acute
Abstract

In this study, the toxicity effects on circulatory system and respiratory system, and the acute toxicity test of recombinant neorudin (EPR-hirudin, EH) in cynomolgus monkeys were evaluated to provide reference information for clinical studies. Eighteen cynomolgus monkeys were randomly divided into three groups for single intravenous administration of 3, 30 mg/kg EH and normal saline, respectively. The changes of respiratory frequency, respiratory intensity, blood pressure and electrocardiogram before and after administration were recorded. In acute toxicity test, six cynomolgus monkeys were intravenously received EH at a single dose of 171, 257, 385, 578, 867 and 1300 mg/kg respectively. The vital signs, hematology, serum biochemistry, coagulation indexes and electrocardiogram indexes of the animals were determined before administration and on the 7th and 14th day after administration. As the results showed that there were no significant abnormal changes in respiratory frequency, respiratory intensity, blood pressure or electrocardiogram in cynomolgus monkeys after receiving EH at 3 mg/kg and 30 mg/kg, and there was no statistical difference between the treated groups and normal saline group. In the acute toxicity test, no significant abnormalities were observed in vital signs, hematology, serum biochemistry, coagulation indexes and electrocardiogram indexes of six cynomolgus monkeys at day 7 and 14 after EH administration. Furthermore, autopsies of all cynomolgus monkeys showed no abnormalities. The results of toxicokinetics showed that AUClast of the drug increased in proportion to the EH dose in the range of 171-578 mg/kg, and increased in over proportion to the EH dose in the range of 578-1300 mg/kg. The variation of Cmax was basically consistent with AUClast. In a sum, A single intravenous injection of 3 and 30 mg/kg of EH did not affect the circulatory system and respiratory system in cynomolgus monkeys and the maximum tolerated dose of EH in cynomolgus monkey is over 1300 mg/kg (equivalent to 619-1300 times of the proposed clinical equivalent dose).

Published
2023
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