The specific root exudate, tripropylene glycol monomethyl ether (TPM), was significantly increased under the maize and peanut intercropping system. Exploring its effects on the growth and metabolic physiology of peanut plants could provide reference for exploring effective substances regulating the growth and development of peanut and promoting the quality and yield improvement of peanut. In this study, Qinghua No.6 peanut variety was used as experimental material, and the same volume of sterile water was added as control (CK). The dry matter accumulation, maximum photochemical efficiency, carbon and nitrogen metabolism enzyme activity and antioxidant properties of peanut plants under three tripropylene glycol monomethyl ether (TPM) adding gradient (T1:10.23μL/pot, T2:20.46μL/pot, T3: 40.92 μL/pot) were studied. The results showed that, compared with CK, T1 and T3 significantly reduced the above-ground dry matter weight of peanut plants. All TPM treatments significantly reduced the dry matter weight of underground part and maximum photochemical efficiency (Fv/Fm) of PSII. TPM supplementation significantly increased the activity of carbon and nitrogen metabolizing enzymes in peanut functional leaves, for detail, the activities of nitrate reductase (NR), sucrose synthetase (SS) and phosphate sucrose synthetase (SPS) in T1 treatment were increased by 6.49%-203.24% compared with CK, and Glutamine synthetase (GS) activity in T3 treatment was significantly increased by 39.81% compared with CK. The supplementation of TPM had stress effect on peanut plants. The content of malondialdehyde (MDA) was significantly increased by 17.32%-34.65% compared with CK. The activities of superoxide dismutase (SOD) and catalase (CAT) under T3 treatment were decreased by 75.82% and 75.74% compared with CK, respectively. The peroxidase (POD) activity under T1 treatment was significantly decreased by 30.43% compared with CK. According to the histochemical localization, T3 treatment significantly induced the accumulation of H2O2 and O2- in peanut plant functional leaves, and the content of O2- was significantly increased by 83.39% compared with CK. In conclusion, TPM can inhibit the growth of peanut plants, especially the underground part, reduce the potential maximum photosynthetic capacity (Fv/Fm) of leaves and inhibit the activities of antioxidant enzymes such as SOD, CAT and POD. However, peanut plants can reduce the damage caused by TPM by improving the activities of carbon and nitrogen metabolic enzymes such as NR, SS, SPS and GS. In this study, TPM is not suitable for peanut production. [ABSTRACT FROM AUTHOR]