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Mercury (Hg) is known to affect aquatic, terrestrial ecosystems as well as human health, through biomagnification. Mangrove wetlands are potential Hg sinks because of their low tidal velocity, fast sedimentation rate, strong reducing condition and high organic matter content. The spatial and temporal distribution of Hg has been a hot topic of recent studies in mangrove wetlands. In this study, we investigated Hg concentration, accumulation rate and isotopes to reconstruct the Hg pollution history and to differentiate its potential sources in the Gaoqiao mangrove wetland (Guangdong province), which is part of the largest mangrove area in China. We reconstructed a first, continuous, high-resolution Hg pollution history over the last 3000 years in South China. Our findings show that mangrove wetland sediments are more enriched in Hg than the adjacent grasslands. The increased Hg concentration and δ 202 Hg in recent sediments mirror the enhanced anthropogenic impacts; Hg concentrations in areas with high levels of anthropogenic disturbance are up to 5× higher than the average background value (9.9 ± 1.2 μg kg -1 ). Compared to mangroves in coastal areas of South China and around the world, the Hg concentration in Gaoqiao is much lower. The significant increase of Hg since the 1950s and the major Hg peak since the 1980s were the evidence of the human activities influences and indicated the possible start date of Anthropocene. After 2007 CE, a decline in Hg pollution occurs due to the effective implementation of the mangrove protection policy. Three potential sources were identified by the Hg isotope traces including urban gaseous Hg, industrial Hg, and regional soil and leaf litter Hg input., Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Kunshan Bao reports financial support was provided by National Natural Science Foundation of China. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Inc. All rights reserved.)

The last decades have witnessed huge efforts devoted to deciphering the pathological mechanisms underlying Alzheimer's Disease (AD) and to testing new drugs, with the recent FDA approval of two anti-amyloid monoclonal antibodies for AD treatment. Beyond these drug-based experimentations, a number of pre-clinical and clinical trials are exploring the benefits of alternative treatments, such as non-invasive stimulation techniques on AD neuropathology and symptoms. Among the different non-invasive brain stimulation approaches, transcranial alternating current stimulation (tACS) is gaining particular attention due to its ability to externally control gamma oscillations. Here, we outline the current knowledge concerning the clinical efficacy, safety, ease-of-use and cost-effectiveness of tACS on early and advanced AD, applied specifically at 40 Hz frequency, and also summarise pre-clinical results on validated models of AD and ongoing patient-centred trials., (© 2024. The Author(s).)

Climate and time are among the most important factors driving soil organic carbon (SOC) stability and accrual in mineral soils; however, their relative importance on SOC dynamics is still unclear. Therefore, understanding how these factors covary over a range of soil developmental stages is crucial to improve our knowledge of climate change impact on SOC accumulation and persistence. Two chronosequences located along a climate gradient were investigated to determine the main interactions among time (age) and climate (precipitation and temperature) on SOC stability and stock with depth. Considering a common depth (0-15 or 0-30 cm), in the drier chronosequence, the older soil showed the highest SOC stock, while the younger exhibited the lowest carbon accumulation. Considering the whole profile, the SOC stock increased with age. In the wetter chronosequence, the younger soil showed the highest SOC stock considering a common depth, whereas, when the entire profile is taken into account, the older one accumulated 2-3 times more SOC than the others. In both chronosequences, significant stocks of SOC (∼42 %) were accumulated below 30 cm. Soil organic matter stability, assessed by thermal analysis and heterotrophic respiration, increases with depth and age only in the drier chronosequence. Soils from the wetter chronosequence were instead characterized by a greater quantity of labile and/or not-stabilized SOC; here, the amorphous Fe/Al-rich secondary mineral weathering products showed an essential predictor function of SOC storage, although they do not seem to be involved in SOC stabilization mechanisms. Otherwise, the interaction of SOC with fine particles, short-range order minerals, and organo-metal complexes represent the significant stabilization mechanisms in soils from drier climate. The results highlighted how the age factor plays an unassuming role in geochemical processes influencing SOC dynamics; however, climate determines different trajectories of soil development and SOC dynamics for a given soil age. Thus, soil age shows a key role in SOC stabilization especially in drier climatic conditions, while wetter conditions determine an accumulation of a higher yet more labile amount of SOC., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)

Peatlands play a crucial role in carbon (C) sequestration and biodiversity conservation. However, these environments are highly vulnerable, and Europe has lost >60 % of its peatland habitat in recent decades. Cattle grazing and trampling contribute to peatland degradation, which generally result in a shift from moss-dominated vegetation to vascular plants and in lower C sequestration rates. Overgrazing poses also a significant threat to habitat integrity and biodiversity, especially in the Alpine area, where close-to-pristine mires with high ecological integrity are becoming extremely rare. Thus, a more in depth understanding of how cattle grazing and trampling are threatening Alpine mires is strongly needed for a sustainable management and conservation of these habitats. The objective of this study was to examine the impact of grazing on the physical, chemical, and biological characteristics of peat, with a focus on diatoms. To answer such a question, seven 50-cm deep cores were collected from mires located in the Adamello-Brenta Nature Park (North of Italy) along a grazing-induced disturbance gradient. Results indicated that grazing primarily affected at least the upper 15 cm of the peat, resulting in increased density and reduced water content, due to compaction, and lower C-to‑nitrogen ratio, possibly caused by both cow manure inputs and increased peat mineralization. Moreover, almost 200 diatom taxa were recorded across the 7 cores, with several of them falling under threat categories in the Red List for central Europe. The higher percentage of eutraphentic species in highly-grazed areas was related to the increase in nutrients caused by cattle manure. Finally, intense grazing increased the share of taxa that are more likely to survive in environments with unstable water availability (= aerial species). We showed that diatom data, supported by physical and chemical parameters, can be a refined tool to inform mire protection and rehabilitation., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024. Published by Elsevier B.V.)

Mangrove wetlands are an important component of blue carbon (C) ecosystems, although the anthropogenic impact on organic C accumulation rate (OCAR) in mangrove wetlands is not yet clear. Three sediment cores were collected from Zhanjiang Gaoqiao Mangrove Reserve in Southern China, dated by 210 Pb and 137 Cs, and physico-chemical parameters measured. Results show that the OCARs in mangroves and grasslands have significantly increased by 4.4 and 1.3 times, respectively, since 1950, which is consistent with the transformation of organic C sources and the increase of sedimentation rate. This increment is due to increased soil erosion and nutrient enrichment caused by land use change and the discharge of fertilizer runoff and aquaculture wastewater. This study provides clear evidence for understanding the changes in organic C accumulation processes during the Anthropocene and is conducive to promoting the realization of C peak and neutrality targets., Competing Interests: The authors declare no competing interests., (© 2024 The Author(s).)

Lignocellulose biomasses (LCB), including spent mushroom substrate (SMS), pose environmental challenges if not properly managed. At the same time, these renewable resources hold immense potential for biofuel and chemicals production. With the mushroom market growth expected to amplify SMS quantities, repurposing or disposal strategies are critical. This study explores the use of SMS for cultivating microbial communities to produce carbohydrate-active enzymes (CAZymes). Addressing a research gap in using anaerobic digesters for enriching microbiomes feeding on SMS, this study investigates microbial diversity and secreted CAZymes under varied temperatures (37 °C, 50 °C, and 70 °C) and substrates (SMS as well as pure carboxymethylcellulose, and xylan). Enriched microbiomes demonstrated temperature-dependent preferences for cellulose, hemicellulose, and lignin degradation, supported by thermal and elemental analyses. Enzyme assays confirmed lignocellulolytic enzyme secretion correlating with substrate degradation trends. Notably, thermogravimetric analysis (TGA), coupled with differential scanning calorimetry (TGA-DSC), emerged as a rapid approach for saccharification potential determination of LCB. Microbiomes isolated at mesophilic temperature secreted thermophilic hemicellulases exhibiting robust stability and superior enzymatic activity compared to commercial enzymes, aligning with biorefinery conditions. PCR-DGGE and metagenomic analyses showcased dynamic shifts in microbiome composition and functional potential based on environmental conditions, impacting CAZyme abundance and diversity. The meta-functional analysis emphasised the role of CAZymes in biomass transformation, indicating microbial strategies for lignocellulose degradation. Temperature and substrate specificity influenced the degradative potential, highlighting the complexity of environmental-microbial interactions. This study demonstrates a temperature-driven microbial selection for lignocellulose degradation, unveiling thermophilic xylanases with industrial promise. Insights gained contribute to optimizing enzyme production and formulating efficient biomass conversion strategies. Understanding microbial consortia responses to temperature and substrate variations elucidates bioconversion dynamics, emphasizing tailored strategies for harnessing their biotechnological potential.

Natural and human-induced environmental changes deeply affected terrestrial ecosystems throughout the Holocene. Paleoenvironmental reconstructions provide information about the past and allow us to predict/model future scenarios. Among potential records, peat bogs are widely used because they present a precise stratigraphy and act as natural archives of highly diverse organic remains. Over the decades, several techniques have been developed to identify debris occurring in peat, including their morphological description. However, this is strongly constrained by the researcher's ability to distinguish residues at the species level, which typically requires many years of experience. In addition, potential contamination hampers using these techniques to obtain information from organisms such as fungi or bacteria. Environmental DNA metabarcoding and shotgun metagenome sequencing could represent a solution to detect specific groups of organisms without any a priori knowledge of their characteristics and/or to identify organisms that have rarely been considered in previous investigations. Moreover, shotgun metagenomics may allow the identification of bacteria and fungi (including both yeast and filamentous life forms), ensuring discrimination between ancient and modern organisms through the study of deamination/damage patterns. In the present review, we aim to i) present the state-of-the-art methodologies in paleoecological and paleoclimatic studies focusing on peat core analyses, proposing alternative approaches to the classical morphological identification of plant residues, and ii) suggest biomolecular approaches that will allow the use of proxies such as invertebrates, fungi, and bacteria, which are rarely employed in paleoenvironmental reconstructions., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier B.V. All rights reserved.)

Atmospheric deposition of polycyclic aromatic hydrocarbons (PAHs) has increased in northern Alberta, Canada, due to industrial development in the Athabasca oil sands region (AOSR). However, the sources, summertime deposition fluxes and associated spatial patterns are poorly characterized, and the magnitude of contamination has not been directly contrasted with comparable measurements around large Canadian cities. PAHs were measured in Sphagnum moss collected from 30 bogs in the AOSR and compared with reference moss collected from various remote, rural and near-urban sites in Alberta and Ontario. At all 39 locations, strong correlations between depositional fluxes of PAHs and accumulation rates of ash (n = 117, r = 0.877, p < 0.001) implied that the main source of PAHs to moss was atmospheric deposition of particles. Average PAH concentrations at near-field AOSR sites (mean [SD], 62.4 [24.3] ng/g) were significantly higher than at far-field AOSR sites (44.9 [20.8] ng/g; p = 0.038) or the 7 reference sites in Alberta (20.6 [3.5] ng/g; p < 0.001). In fact, average PAH concentrations across the entire AOSR (7,850 km 2 ) were approximately twice as high as in London, Ontario, or near petroleum upgrading and major traffic corridors in Edmonton, Alberta. A chemical mass balance model estimated that both delayed petcoke (33 % of PAHs) and fine tailings (38 % of PAHs) were the major sources of PAHs in the AOSR. Over the 2015 summer growing season, we estimate that 101-110 kg of PAHs (on 14,300-17,300 tonnes of PAH-containing dusts) were deposited to the AOSR within a 50 km radius of surface mining. Given that the highest PAH deposition was to the northern quadrant of the AOSR, which includes the First Nations community of Fort MacKay, further dust control measures should be considered to protect human and environmental health in the region., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 The Author(s). Published by Elsevier Ltd.. All rights reserved.)

Lignohumates are increasing in popularity in agriculture, but their chemistry and effects on plants vary based on the source and processing. The present study evaluated the ability of two humates (H1 and H2) to boost maize plant performance under different phosphorus (P) availability (25 and 250 μM) conditions in hydroponics, while understanding the underlying mechanisms. Humates differed in chemical composition, as revealed via elemental analysis, phenol and phytohormone content, and thermal and spectroscopic analyses. H1 outperformed H2 in triggering plant responses to low phosphorus by enhancing phosphatase and phytase enzymes, P acquisition efficiency, and biomass production. It contained higher levels of endogenous auxins, cytokinins, and abscisic acid, likely acting together to stimulate plant growth. H1 also improved the plant antioxidant capacity, thus potentially increasing plant resilience to external stresses. Both humates increased the nitrogen (N) content and acted as biostimulants for P and N acquisition. Consistent with the physiological and biochemical data, H1 upregulated genes involved in growth, hormone signaling and defense in all plants, and in P recycling particularly under low-P conditions. In conclusion, H1 showed promising potential for effective plant growth and nutrient utilization, especially in low-P plants, involving hormonal modulation, antioxidant enhancement, the stimulation of P uptake and P-recycling mechanisms.

The sustainability of Mediterranean croplands is threatened by climate warming and rainfall reduction. The use of biochar as an amendment represents a tool to store organic carbon (C) in soil. The vulnerability of soil organic C (SOC) to the joint effects of climate change and biochar application needs to be better understood by investigating its main pools. Here, we evaluated the effects of partial rain exclusion (∼30%) and temperature increase (∼2 °C), combined with biochar amendment, on the distribution of soil organic matter (SOM) into particulate organic matter (POM) and the mineral-associated organic matter (MAOM). A set of indices suggested an increase in thermal stability in response to biochar addition in both POM and MAOM fractions. The MAOM fraction, compared to the POM, was particularly enriched in labile substances. Data from micro-Raman spectroscopy suggested that the POM fraction contained biochar particles with a more ordered structure, whereas the structural order decreased in the MAOM fraction, especially after climate manipulation. Crystalline Fe oxides (hematite) and a mix of ferrihydrite and hematite were detected in the POM and in the MAOM fraction, respectively, of the unamended plots under climate manipulation, but not under ambient conditions. Conversely, in the amended soil, climate manipulation did not induce changes in Fe speciation. Our work underlines the importance of discretely taking into account responses of both MAOM and POM to better understand the mechanistic drivers of SOC storage and dynamics., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier Ltd. All rights reserved.)

Volcanic eruptions generate huge amounts of material with a wide range of compositions and therefore different physicochemical properties. We present a combined Raman and calorimetric study carried out on four synthetic basaltic glasses with different alkali vs iron ratio which spans the typical compositions of basalts on Earth. Differential scanning calorimetry shows that changes of this ratio modify the glass transition interval whereas Raman spectra allow to gain insight about the structure of the glass in the microscopic and macroscopic range. Indeed, our Raman analysis is extended from the high frequency region, characterized by the molecular peaks, to the very low frequency region where glasses exhibit the boson peak. Spectra show a variation of the non-bridging oxygens number that affects the medium range order of the glass and the network interconnections. In the considered substitution interval, the boson peak shape is conserved while its position shift upwards. This means that increasing the alkali vs iron content, the elastic medium hardens but it does not change nature. This study emphasizes the importance of considering the full-range spectra when analysing multicomponent or natural systems with small chemical variations., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier B.V. All rights reserved.)

In this study, hydrochar (HC), a carbon-rich product originated from hydrothermal conversion treatment (HTC), was obtained from wastes of the wine and dairy industries. The effect of mixing secondary char and compost was tested, before and after the aerobic mixing of compost (COM) and HC at increasing doses (from 15 to 75 Mg ha -1 DM), in an effort to lower the HC phytotoxicity due to potential phytotoxic compounds of secondary char. The results indicated that, after the aerobic stabilization, the mix HC/COM was able to double the plant growth in comparison to COM alone. The presence of easily degradable organic compounds probably led to poor stability of HC, increased microbial activity and, consequently, root anoxia when used at high doses. Chemical, spectroscopic and thermal investigation confirmed this hypothesis. In particular, HC shows a high content of dissolved organic matter, characterized by the presence of small molecules, which is negatively correlated with the growth index of lettuce. Furthermore, thermal characterization suggests a higher proportion of less complex and thermally stable molecular compounds in HC in comparison to COM. Therefore, co-composting of HC allows obtaining a useful amendment to support soil organic matter and fertility., Competing Interests: Declaration of Competing Interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Daniela Bona reports financial support was provided by Fondazione Valorizzazione Ricerca Trentina., (Copyright © 2023 Elsevier Ltd. All rights reserved.)

Thauera is one of the main genera involved in polyhydroxyalkanoate (PHA) production in microbial mixed cultures (MMCs) from volatile fatty acids (VFAs). However, no Thauera strains involved in PHA accumulation have been obtained in pure culture so far. This study is the first report of the isolation and characterization of a Thauera sp. strain, namely Sel9, obtained from a sequencing batch reactor (S-SBR) set up for the selection of PHA storing biomass. The 16S rRNA gene evidenced a high sequence similarity with T. butanivorans species. Genome sequencing identified all genes involved in PHA synthesis, regulation and degradation. The strain Sel9 was able to grow with an optimum of chemical oxygen demand-to-nitrogen (COD:N) ratio ranging from 4.7 to 18.9. Acetate, propionate, butyrate and valerate were used as sole carbon and energy sources: a lag phase of 72 h was observed in presence of propionate. Final production of PHAs, achieved with a COD:N ratio of 75.5, was 60.12 ± 2.60 %, 49.31 ± 0.7 %, 37.31 ± 0.43 % and 18.06 ± 3.81 % (w/w) by using butyrate, acetate, valerate and propionate as substrates, respectively. Also, the 3-hydroxybutyrate/3-hydroxyvalerate ratio reflected the type of carbon sources used: 12.30 ± 0.82 for butyrate, 3.56 ± 0.02 for acetate, 0.93 ± 0.03 for valerate and 0.76 ± 0.02 for propionate. The results allow a better elucidation of the role of Thauera in MMCs and strongly suggest a possible exploitation of Thauera sp. Sel9 for a cost-effective and environmentally friendly synthesis of PHAs using VFAs as substrate., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 The Authors. Published by Elsevier B.V. All rights reserved.)

We report the first example of very efficient NIR Circularly Polarized Luminescence (CPL) (around 970 nm) in water, obtained thanks to the combined use of a chiral Yb complex and of poly lactic-co-glycolic acid (PLGA) nanoparticles. [YbL(tta) 2 ]CH 3 COO (L=N, N'-bis(2-pyridylmethylidene)-1,2-(R,R+S,S) cyclohexanediamine and tta=2-thenoyltrifluoroacetonate) shows good CPL in organic solvents, because the tta ligands efficiently sensitize Yb NIR luminescence and the readily prepared chiral ligand L endows the complex with the necessary dissymmetry. PLGA nanoparticles incorporate the complex and protect the metal ion from the intrusion of solvent molecules, while ensuring biocompatibility, water solubility and stability to the complex. Hydrophilic NIR-CPL optical probes can find applications in the field of NIR-CPL bio-assays., (© 2022 The Authors. Chemistry - A European Journal published by Wiley-VCH GmbH.)

A 3-m thick sediment sequence, found in a limestone mine located in the south of Italy at a depth of ca. 25-30 m from the current ground level, was investigated. Samples from 5 layers were analysed by X-ray diffraction, elemental analysis, Inductively Coupled Plasma Mass Spectrometry and micromorphology. Microbial DNA was analysed by 16S rRNA gene metabarcoding. The main mineral compounds found in the 5 layers were calcite (70-80%) and clay minerals in layers #1 and #5, goethite (75%) and hematite in layer #2, manganese (66%) and iron oxides in layer #3, and almost exclusively goethite in layer #4. Micromorphology data allowed to shed light in understanding whether these sediments formed by subsequent weathering of carbonates and silicates or by migration of soil sediments from the surface, or also by the accumulation of shallow marine sediments occurring between the middle Pliocene and the lower Pleistocene, when the extreme western sector of this area underwent strong subsidence. From the microbiological point of view, upon the 16S rRNA gene analysis, these 5 layers appear to cluster in three groups. Overall, such a distribution suggests that, both in the top (#1) and in bottom layers (#4 and #5), different communities would have undergone in situ reproduction and colonization exploiting metabolically the substrate, whereas the two mid layers would have received bacterial convection by passive transport of percolating waters. At the same time, micromorphological data show that each layer preserved its distinct features to be related to the environmental condition at the time of deposition. The chemical, mineralogical and micromorphological features of the layers and the known physiology of the microbial taxa thereby encountered highlight the possible role of the latter in elucidating the occurrence of certain mineral species as well as the biogeochemistry of elements like Mn and Fe in sediment layers., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2021 Elsevier B.V. All rights reserved.)