Search

Your search keyword '"Zhang, Aying"' showing total 9 results
Start Over Author "Zhang, Aying" Publication Year Range Last 3 years
9 results on '"Zhang, Aying"'

9. ZmWRKY104 positively regulates salt tolerance by modulating ZmSOD4expression in maize

Author

Yan, Jingwei, Li, Jing, Zhang, Heping, Liu, Ya, and Zhang, Aying

Abstract

Salinity impairs plant growth, limiting agricultural development. It is desirable to identify genes responding to salt stress and their mechanism of action. We identified a function of the Zea maysWRKY transcription factor, ZmWRKY104, in salt stress response. ZmWRKY104 was localized in the nucleus and showed transcriptional activation activity. Phenotypic and physiological analysis showed that overexpression of ZmWRKY104in maize increased the tolerance of maize to salt stress and alleviated salt-induced increases in O2−accumulation, malondialdehyde (MDA) content, and percent of electrolyte leakage. Further investigation showed that ZmWRKY104 increased SOD activity by regulating ZmSOD4expression. Yeast one-hybrid, electrophoretic mobility shift test, and chromatin immunoprecipitation–quantitative PCR assay showed that ZmWRKY104 bound directly to the promoter of ZmSOD4by recognizing the W-box motif in vivoand in vitro. Phenotypic, physiological, and biochemical analysis showed that ZmSOD4 increased salt tolerance by alleviating salt-induced increases in O2−accumulation, MDA content, and percent of electrolyte leakage under salt stress. Taken together, our results indicate that ZmWRKY104 positively regulates ZmSOD4expression to modulate salt-induced O2−accumulation, MDA content, and percent of electrolyte leakage, thus affecting salt stress response in maize.

Published
2022
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results