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Background: G-protein coupled receptors (GPCRs) are recognized as attractive targets for drug therapy. However, it remains poorly understood how GPCRs, except for a few chemokine receptors, regulate the progression of liver fibrosis. Here, we aimed to reveal the role of GPR65, a proton-sensing receptor, in liver fibrosis and to elucidate the underlying mechanism., Methods: The expression level of GPR65 was evaluated in both human and mouse fibrotic livers. Furthermore, Gpr65-deficient mice were treated with either bile duct ligation (BDL) for 21 d or carbon tetrachloride (CCl 4 ) for 8 weeks to investigate the role of GPR65 in liver fibrosis. A combination of experimental approaches, including Western blotting, quantitative real-time reverse transcription‑polymerase chain reaction (qRT-PCR), and enzyme-linked immunosorbent assay (ELISA), confocal microscopy and rescue studies, were used to explore the underlying mechanisms of GPR65's action in liver fibrosis. Additionally, the therapeutic potential of GPR65 inhibitor in the development of liver fibrosis was investigated., Results: We found that hepatic macrophages (HMs)-enriched GPR65 was upregulated in both human and mouse fibrotic livers. Moreover, knockout of Gpr65 significantly alleviated BDL- and CCl 4 -induced liver inflammation, injury and fibrosis in vivo, and mouse bone marrow transplantation (BMT) experiments further demonstrated that the protective effect of Gpr65 knockout is primarily mediated by bone marrow-derived macrophages (BMMs). Additionally, in vitro data demonstrated that Gpr65 silencing and GPR65 antagonist inhibited, while GPR65 overexpression and application of GPR65 endogenous and exogenous agonists enhanced the expression and release of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and transforming growth factor-β (TGF-β), all of which subsequently promoted the activation of hepatic stellate cells (HSCs) and the damage of hepatocytes (HCs). Mechanistically, GPR65 overexpression, the acidic pH and GPR65 exogenous agonist induced up-regulation of TNF-α and IL-6 via the Gαq-Ca 2+ -JNK/NF-κB pathways, while promoted the expression of TGF-β through the Gαq-Ca 2+ -MLK3-MKK7-JNK pathway. Notably, pharmacological GPR65 inhibition retarded the development of inflammation, HCs injury and fibrosis in vivo., Conclusions: GPR65 is a major regulator that modulates the progression of liver fibrosis. Thus, targeting GPR65 could be an effective therapeutic strategy for the prevention of liver fibrosis., (© 2023. The Author(s).)

A new species Rosafuningensis and its variant R.funingensisf.rosea, both collected from Yunnan Province, China, are, for the first time, documented and illustrated in this study. Morphological analysis in comparison with two related species in the wild, R.gigantea and R.rubus , presents distinguishable features through leaf surfaces, inflorescences and the shape of styles. R.funingensis leaf surfaces are abaxially villous, purple-red, pale green when mature, adaxially glabrous, dark green; inflorescences solitary or 2-5(7) in corymbose cyme; and styles connate into a column or not, exserted., Competing Interests: The authors have declared that no competing interests exist., (Ling-Na Zheng, Le Luo, Yu-Wei Tang, Chao Yu, Pei-Feng Lyu, Xue-Sen Liu, Qi-Xiang Zhang, Yu-Yong Yang.)

Objective: The aim of this study was to evaluate the application value of lung ultrasound in monitoring bronchopulmonary dysplasia (BPD) and pulmonary artery pressure in premature infants., Patients and Methods: A total of 98 preterm infants diagnosed with BPD in the Fourth Hospital in Shijiazhuang were recruited, and their disease severity was classified as mild (n=32), moderate (n=33), or severe BPD (n=33) based on gestational age and oxygen concentration. Lung ultrasonography of the children was performed. The correlation between lung ventilation scores and disease severity was statistically analyzed, and the discrete optimization results were documented. The pulmonary hypertension indexes of the three groups of children were compared., Results: Aberrant alterations of the pleural line were observed in all included children, and the B-line rose as the disease progressed. The duration of invasive ventilation, medication, and hospital stay increased with disease exacerbation (p<0.05). The three groups significantly differed in terms of ultrasound pulmonary ventilation scores and clinical severity (p<0.05). Only mild BDP was identified by lung ultrasound on the first day of birth (T1), and severe BDP was detectable during the first and second week (T2-T3) as well as the third and fourth week (T4-T5). Severe BPD was associated with significantly higher levels of pulmonary hypertension indices vs. mild and moderate BPD (p<0.05)., Conclusions: Pulmonary ultrasonography demonstrates great potential to predict pulmonary hypertension in children and assesses the disease severity. Pulmonary ultrasound allows for dynamical real-time observation of the pulmonary lesions in children with pulmonary hypertension, thereby revealing the severity of pulmonary hypertension in premature children.

Aim: To make a bibliometric analysis on post-traumatic growth (PTG) after childbirth., Methods: The topic advanced search strategy extracted the information from the Web of Science Core Collection. Descriptive statistics were performed using Excel, and bibliometric analysis was performed using VOSviewer., Results: A total of 362 publications were published in 199 journals were obtained in the WoSCC from 1999 to 2022. Postpartum post-traumatic growth is in a trend of fluctuating growth, and the United States (N = 156) and Bar-Ilan University (N = 22) were the top contributing countries and institutions, respectively. Research hotspots mainly focus on theoretical models of PTG, postpartum post-traumatic stress disorder (PTSD) as a predictor of PTG, facilitators of PTG, and the relationship between mother-infant attachment and PTG., Conclusion: This bibliometric study provides a comprehensive overview of the current state of research on PTG after childbirth, an area that has received considerable scholarly attention in recent years. However, research on post-traumatic growth after childbirth is lacking, and further research is needed., (© 2023 Japan Society of Obstetrics and Gynecology.)

Objectives: To investigate the satisfaction of the undergraduate nursing classroom teaching quality based on the Kano model and provide a reference for improving the overall quality of educational services., Methods: A total of 621 sophomores and juniors of nursing at Hubei University of Medicine were selected to conduct questionnaires based on the Kano model using convenient sampling., Results: The 16 items are all a one-dimensional factor. The satisfaction and importance are 0.77 to 0.86 and -0.58 to -0.80, respectively. In the quadrant analysis chart, there are four items in the dominant area, three items for improvement, three in the observation area, and six in the retention area., Conclusion: Analyzing the satisfaction of nursing undergraduates' classroom teaching quality can help us effectively understand the intrinsic needs of students and improve the overall quality of nursing undergraduates' curriculum teaching services., Competing Interests: The author(s) declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article., (© The Author(s) 2023.)

To reasonably design and synthesize metal-organic frameworks (MOFs) with high stability and excellent adsorption/separation performance, the pore configuration and functional sites are very important. Here, we report two structurally similar cluster-based MOFs using a pyridine-modified low-symmetry ligand [H 4 L = 2,6-bis(2',5'-dicarboxyphenyl)pyridine], [(NH 2 Me 2 ) 2 ][Co 5 (L) 2 (OCH 3 ) 2 (μ 3 -OH) 2 ·2DMF]·2DMF·2H 2 O ( 1 ) and [Co 5 (L) 2 (μ 3 -OH) 2 (H 2 O) 2 ]·2H 2 O·4DMF ( 2 ). The structures of 1 and 2 are built from Co 5 clusters, which have one-dimensional open channels, but their microporous environments are different due to the different ways in which ligands bind to the metals. Both MOFs have extremely high chemical stabilities over a wide pH range (2-12). The two MOFs have similar adsorption capacities of C 2 H 2 (144.0 cm 3 g -1 for 1 and 141.3 cm 3 g -1 for 2 ), but 1 has a higher C 2 H 2 /CO 2 selectivity of 3.5 under ambient conditions. The difference in gas adsorption and separation between the two MOFs has been compared by a breakthrough experiment and theoretical calculation, and the influence of the microporous environment on the gas adsorption and separation performance of MOFs has been further studied.

An anionic three-dimensional framework {(Me 2 NH 2 ) 2 [Zn 8 (L) 6 (ad) 4 (μ 4 -O)]·10DMF·11H 2 O}(Zn-MOF, L 2- = 4,4'-(3-aminopyridine-2,5-diyl)dibenzoic acid; ad - = adeninate) with a column-layered structure was synthesized. Structural studies show that the Zn-MOF has octahedral cages [Zn 8 (ad) 4 (μ 4 -O)], the adjacent cages are connected by O atoms to form 1D columnar SBUs, and every four SBUs are connected by L 2- to form a square channel 3D framework. Gas adsorption studies show that the BET and Langmuir surface areas of Zn-MOF are 1370.31 and 1478.04 m 2 g -1 , respectively, and the total pore volume for single-point adsorption is 0.528 cm 3 g -1 . The surface of the pores of the Zn-MOF is occupied by open metal sites and uncoordinated carboxyl groups, showing good capture ability for C 2 H 2 and good adsorption selectivity for C 2 H 2 /CH 4 . More importantly, the free (CH 3 ) 2 NH 2 + ions present in the pores of the columnar layered Zn-MOF can cation-exchange with MB, MV, and RhB ions in aqueous solution. Therefore, Zn-MOF can not only effectively adsorb the cationic dyes MB, MV, and RhB, but also exhibit particularly selective sorption towards the mixed anion and cation dyes MB/MO and MV/MO. In addition, a series of fluorescence experiments show that Zn-MOF has good fluorescence properties, exhibiting highly selective and sensitive fluorescence detection and recognition performance for Fe 3+ ions in aqueous solution with a low detection limit.

Objectives: To explore the influencing factors of the horizontal distance of bodies in the high falling scene and the feasibility of inferring the falling mode based on it., Methods: A total of 614 high falling deaths and 15 cases of corpse dumping from high altitudes were collected. The relationship between the horizontal distance and the falling height, as well as the sex, age and manner of death (suicide, accident and corpse dumping) were observed., Results: The horizontal distance increased with the increase of falling height, and the difference among the height groups was statistically significant. The horizontal distance decreased with the increase of the age of the deceased, in each height group, the difference between the group over 60 years old and other age groups was statistically significant ( P <0.05). The horizontal distance of male deceased was (1.99±0.27) m, which was greater than that of female deceased (1.88±0.19) m, and the difference was statistically significant in partial height groups ( P <0.05). Roof falls had a greater horizontal movement distance than window falls. Except for the >20-30 m group, there was no significant difference in horizontal distance between suicide high falls and accidental high falls in other height groups., Conclusions: The horizontal distance is affected by the falling height, the sex and age of the victim, and the spatial characteristics of the falling starting point.