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School lunch plays an important role in children's healthy growth. Previous investigations revealed many problems with school lunches, including unreasonable dietary structure and insufficient micronutrients. This study aimed to assess the dietary structure and nutritional quality of lunches in Nanjing primary and middle schools. A stratified cluster random sampling method was used to select 44 schools that supply lunch in 12 districts in Nanjing, with two primary and two middle schools in each district. Twenty-four primary and twenty middle schools were selected. The Mann–Whitney U test was used to explore the influencing factors. Findings revealed a serious shortage of milk and fruit in school lunches; supply of eggs, fish, shrimp, and shellfish was less than half of the recommended quantity; livestock and poultry supply exceeded the recommended level by over four times. Energy and nutrition intake were suboptimal. Provision of energy, carbohydrates, vitamins (A, B1, B2, and C), calcium, and iron in urban primary schools was significantly higher than that in non-urban primary schools. The same pattern of significantly higher nutrients was equally seen in urban middle schools compared with non-urban middle schools, indicating that food supply was affected by regional economies. Therefore, it is urgent to improve the quality of lunches, with a particular focus on those in non-urban areas. [ABSTRACT FROM AUTHOR]

Lablab purpureus is a crop resource with huge potential as food and feed in tropical agricultural production systems. However, it has not been utilized to its full potential. In this paper, we assessed the genetic variation in the nutritional composition; crude protein (CP), ether extracts (EE), crude fiber (CF), moisture, ash, nitrogen free extracts (NFE), metabolizable energy (ME), neutral detergent fiber (NDF), acid detergent fiber (ADF) and dry matter (DM) and mineral composition of a collection of 51 Lablab accessions. Genotype variance explained 76.7% for CP and 89% for ash (total mineral elements) of the total variance. There were highly significant differences in accessions for all nutritional and mineral components. Results showed that both nutritional and mineral components are highly heritable. However, among the nutritional components, EE had the lowest heritability (68%), while for mineral components, Zn had the lowest heritability (18%). Correlations among nutritional and mineral components were high, 0.91 and 0.94, respectively. There was significant correlation between all mineral elements. Crude protein had the highest negatively significant correlation. Indirect selection for high crude protein has a trade off on NFE. Genotype T47 exhibited the highest values for proximate and mineral composition. Cluster analysis of proximate components revealed two major groups, while mineral components were divided in three major groups, guiding the trade off in breeding. The data show that accession T47 can be used for crude protein improvement of the Lablab population and thus boost the development and production of protein dense Lablab for food and feed. [ABSTRACT FROM AUTHOR]

Background: A significant impediment to the advancement of animal husbandry in India is the insufficient availability of green fodder throughout the year. The nutritive quality of forages is heavily influenced by farm-level management practices. Consequently, there is a pressing need to enhance productivity through the implementation of proper agronomic practices. The nutrient management in fodder-based cropping sequence is a key to maximize the fodder production and its quality. Methods: A field experiment was conducted using a randomized block design, consisting of twelve treatments incorporating various organic sources of nutrients. The experiment was replicated thrice during Kharif and Rabi season 2020-22 at Department of Forage Crops, Tamil Nadu Agricultural University, Coimbatore. The treatments included different proportions (100, 75, 50 and 25%) of the recommended dose of nitrogen applied through FYM, vermicompost and bio compost. Result: The experiment results of the three years study revealed that application of 50% RDN through FYM+ 50% RDN through vermicompost recorded the higher green fodder yield of 691 q/ha, dry matter yield of 130.7 q/ha and crude protein yield of 19.3 q/ha. Correlation and regression analysis also indicated that the growth and yield attributes had a positive impact on the fodder yield. Hence it can be recommended that application of FYM and vermicompost (50 and 50% RDN) found better source of organic nutrients for achieving sustainable and economical fodder yield with quality in fodder cowpea-maize cropping system. [ABSTRACT FROM AUTHOR]

Papaya (Carica papaya L.) is a climacteric fruit with a resilient and distinctive aroma. The consumption of the fruit is global due to its high nutritive and medicinal values. However, there has been low production of quality papaya fruits due to unavailability of crucial mineral elements in the soils. The current study therefore, focuses on the effect of indigenous arbuscular mycorrhizal fungi (AMF) as a biofertilizer on the quality of papaya fruits. In order to verify AMF effectiveness on papaya fruits, four treatments were used: AMF inoculum only, composted farm yard manure (FYM) only, combination of AMF inoculum and compost FYM and control where only soil and sand media were used at a ratio of 1:1. Jomo Kenyatta University of Agriculture and Technology (JKUAT) and Malkia papaya hybrids were used. The papaya seeds from JKUAT and Malkia papaya hybrids were sown in trays and transplanted at 3 leaves stage into 5 litre pots within a green house. The AMF spores were bulked using sorghum plants to obtain the AMF inoculum. The treatments were added into the soil media of the papaya plantlets at a ratio of 1:3, every 4 weeks after first transplanting until they were 20 weeks old. They were then transplanted to 100 litre containers, where completely randomized design was used and replication of six papaya plants for each treatment and hybrid. Watering, weeding and cooling the green house with water fumes was carried out when necessary; as the papaya plants grew until the fruits attained physiological maturity. The fruits were separately harvested and ripened to a predetermined stage. They were then analysed for moisture content, crude fibre, minerals (nitrogen, phosphorous, potassium, magnesium, calcium, iron and zinc), ascorbic acid, total carotenoids and total polyphenols. Data obtained was subjected to twoway ANOVA at p = 0.05 significance level; means were separated using Tukey's HSD test in Genstat's 15th edition. JKUAT hybrid with AMF inoculum treatment had 3.07%crude fibre and 8.42mg/100g phosphorous content while JKUAT hybrid with both AMF inoculum and manure treatments had 4.9 % crude fibre and 9.88 mg/100g phosphorous content. Malkia and JKUAT hybrids with compost FYM treatment had potassium content of 98.31mg/100g and 109.4 mg/100g respectively while the controls had 31.58 mg/100g and 35.32mg/100g respectively. Incorporating soil media with manure and AMF inoculum improved the nutritive quality of papaya fruits and this was contingent on papaya hybrids. [ABSTRACT FROM AUTHOR]

This study aimed to evaluate the dry matter yield, nutrient composition, in vitro and methane produced from Megathyrsus maximus (Ntchisi) and Cenchrus purpureus grazed at two grazing frequency (3 and 6-week, (GF)) with two plant spacing (0.5 m x 1 m and 1 m x 1 m). The design of this experiment was split-split plot design in 2 x 2 x 2 factorial arrangement. Milled samples were analyzed for chemical composition, In vitro dry matter digestibility (IVDMD), total gas production, methane (CH4) production, total volatile fatty acid content, content of acetate, butyrate, propionate and acetate: propionate ratio. The M. maximus grazed at 6-week GF with 1 m x 1 m spacing had the highest dry matter yield. The crude protein (CP) content of the grasses ranged from 104.24 – 181.35 g/kg DM with C. purpureus grazed at 3-week with 1 m x 1 m spacing ranked highest in CP content and protein fraction A, B1, and B2. Megathyrsus maximus at 6-week GF with 1 m x 1 m spacing produced the highest content of acid detergent fibre. Metabolizable energy (ME), short chain fatty acid and volume of gas produced at 48 h of incubation was highest for M. maximus grazed at 3-week GF with 0.5 m x 1 m spacing. Megathyrsus maximus at 3 and 6-week GF with 0.5 m x 1 m spacing displayed highest CH4 value. Acetate, propionate, and total volatile fatty acid were significantly (P

Freeze-drying is an excellent method for dehydration due to its benefits, including increased shelf-life, unique texture, and, in particular, good nutritive quality. However, the applicability of traditional freeze-drying systems in the food industry is still challenging owing to their prolonged drying duration, extraordinary energy usage, and high process cost. Therefore, the need to upgrade or develop conventional freeze-dryers for common or sophisticated food structures is ever-increasing. Enhancements to the freeze-drying process can significantly speed up drying and reduce energy consumption while maintaining phytochemicals, physical quality, and sensory attributes in final products. To overcome the downsides of conventional freeze-drying, hybrid freeze-drying methods were introduced with a great potential to provide food products at shorter drying durations, lower costs, and environmental friendliness while resulting in the same nutritive and sensory qualities as that of conventional freeze-drying in special circumstances. An overview of the most current improvements, adaptations, and applications of hybrid freeze-drying in food dehydration is given here. In this review, comparative studies are offered to characterize the drying process from the standpoint of chemical quality and sensory attributes. All the reviewed studies confirmed that the nutritional and sensory qualities of the end product can be retained using hybrid freeze-drying almost to the same extent as using single freeze-drying. It was also inferred that hybrid freeze-drying can surpass conventional freeze-drying and allow for obtaining dried products with characteristics typical of raw material if operating parameters are optimized based on product quality and energy usage. [ABSTRACT FROM AUTHOR]

Barley is an important source of b-glucans because they have a positive influence on human health and potential application in the food industry. The aim of this manuscript was to determine the variability of ten barley cultivars based on b-glucan content. The percentage of b-glucan varied in the range of 4.44% to 8.17%, with a mean value of 6.57%. Based on b-glucan content, the degree of cultivar similarity was determined by the dendrogram. Among analyzed barley cultivars, the highest concentration of b-glucan was found in the cultivar Lav and Ukras, while the lowest concentration was observed in the cultivar Vihor (4.44%). Compared with all the other cultivars mentioned, a pair of cultivars expressed the greatest distance. Statistical analysis showed that there is a significant difference between analyzed barley cultivars. The analysis of b-glucans in varieties of barley in two growing seasons aims to develop an agricultural strategy and develop barley varieties with higher b-glucans content.

This study aimed to evaluate the dry matter yield, nutrient composition, in vitro and methane produced from Megathyrsus maximus (Ntchisi) and Cenchrus purpureus grazed at two grazing frequency (3 and 6-week, (GF)) with two plant spacing (0.5 m x 1 m and 1 m x 1 m). The design of this experiment was split-split plot design in 2 x 2 x 2 factorial arrangement. Milled samples were analyzed for chemical composition, In vitro dry matter digestibility (IVDMD), total gas production, methane (CH4) production, total volatile fatty acid content, content of acetate, butyrate, propionate and acetate: propionate ratio. The M. maximus grazed at 6-week GF with 1 m x 1 m spacing had the highest dry matter yield. The crude protein (CP) content of the grasses ranged from 104.24 - 181.35 g/kg DM with C. purpureus grazed at 3-week with 1 m x 1 m spacing ranked highest in CP content and protein fraction A, B1, and B2. Megathyrsus maximus at 6-week GF with 1 m x 1 m spacing produced the highest content of acid detergent fibre. Metabolizable energy (ME), short chain fatty acid and volume of gas produced at 48 h of incubation was highest for M. maximus grazed at 3-week GF with 0.5 m x 1 m spacing. Megathyrsus maximus at 3 and 6-week GF with 0.5 m x 1 m spacing displayed highest CH4 value. Acetate, propionate, and total volatile fatty acid were significantly (P<0.05) highest for M. maximus grazed at 6-week with 0.5 m x 1 m spacing. [ABSTRACT FROM AUTHOR]

Barley is an important source of ß-glucans because they have a positive influence on human health and potential application in the food industry. The aim of this manuscript was to determine the variability of ten barley cultivars based on ß-glucan content. The percentage of ß-glucan varied in the range of 4.44% to 8.17%, with a mean value of 6.57%. Based on ß-glucan content, the degree of cultivar similarity was determined by the dendrogram. Among analyzed barley cultivars, the highest concentration of ß-glucan was found in the cultivar Lav and Ukras, while the lowest concentration was observed in the cultivar Vihor (4.44%). Compared with all the other cultivars mentioned, a pair of cultivars expressed the greatest distance. Statistical analysis showed that there is a significant difference between analyzed barley cultivars. The analysis of ß-glucans in varieties of barley in two growing seasons aims to develop an agricultural strategy and develop barley varieties with higher ß-glucans content. [ABSTRACT FROM AUTHOR]

Nanotechnology has the potential to be used in the food business and processing as novel pathogen detection instruments, disease treatment delivery methods, food packaging, and bioactive component distribution to specific areas. Nanotechnology's implementation in food systems will bring new approaches to improving food safety and nutritional value. It sums up the capability of nanoparticles for their utilization in the food business to give purchasers a safe and tainting-free food and to guarantee the customer adequacy of the food with upgraded useful properties. With the increase in shelf life and enhanced quality, the edible packages or thin-film usage can delay the deterioration of food. To regulate the nanomaterials and applications in the food industry a legal basis has been made. The Organization of Economic Co-operation and Development (OECD) recommended the standard test guidelines be used for the hazard assessment of nanomaterials for chemical safety. Finally, nanotechnology supports the change of the existing food processing systems to attest the safety of the products, nurturing a healthy food, and also the food's nutritive quality to be enhanced. The straightforwardness of security issues and natural effect should be the need while managing the advancement of nanotechnology in food frameworks and hence mandatory testing of nano food varieties is expected before they are delivered to the market. [ABSTRACT FROM AUTHOR]

Freeze-drying is an excellent method for dehydration due to its benefits, including increased shelf-life, unique texture, and, in particular, good nutritive quality. However, the applicability of traditional freeze-drying systems in the food industry is still challenging owing to their prolonged drying duration, extraordinary energy usage, and high process cost. Therefore, the need to upgrade or develop conventional freeze-dryers for common or sophisticated food structures is ever-increasing. Enhancements to the freeze-drying process can significantly speed up drying and reduce energy consumption while maintaining phytochemicals, physical quality, and sensory attributes in final products. To overcome the downsides of conventional freeze-drying, hybrid freeze-drying methods were introduced with a great potential to provide food products at shorter drying durations, lower costs, and environmental friendliness while resulting in the same nutritive and sensory qualities as that of conventional freeze-drying in special circumstances. An overview of the most current improvements, adaptations, and applications of hybrid freeze-drying in food dehydration is given here. In this review, comparative studies are offered to characterize the drying process from the standpoint of chemical quality and sensory attributes. All the reviewed studies confirmed that the nutritional and sensory qualities of the end product can be retained using hybrid freeze-drying almost to the same extent as using single freeze-drying. It was also inferred that hybrid freeze-drying can surpass conventional freeze-drying and allow for obtaining dried products with characteristics typical of raw material if operating parameters are optimized based on product quality and energy usage.

Summary: The effects of germination time on phenolic, flavonoid, anthocyanin, antioxidant capacities, inhibition capacity of α‐amylase, bioaccessibility of phenolic and digestibility of starch in sorghum were evaluated in this study. The levels of total phenolic, flavonoid and anthocyanin in germinated sorghum for 48 h increased by 39.74%, 37.28% and 52.21%, respectively. Germination also increased the composition of phenolic, flavonoid and anthocyanin in sorghum, and their antioxidant capacity and inhibitory rates of α‐amylase. Additionally, in vitro digestion results showed that phenolic bioaccessibility increased by 10.18%, and digestibility of starch and expected glycaemic index (eGI) decreased by 13.87% and 5.26 in germinated sorghum for 48 h compared with the ungerminated sorghum. These results indicate that germination might be a green method to improve the nutritional quality of sorghum and promote the development of germinated whole‐grain food. [ABSTRACT FROM AUTHOR]

Potatoes (Solanum tuberosum L.) are the fourth most economically important crop in the world. They have a short period of vegetation and are an excellent source of carbohydrates, amino acids, vitamins, organic acids, minerals and phenolics as antioxidant substances. Potato can be a major dietary source of various bioactive compounds. In this study, we applied gas chromatography coupled with mass spectrometry (GC-MS) metabolite profiling to classify eight Bulgarian potato cultivars bred in the Maritsa Vegetable Crops Research Institute (VCRI), Plovdiv, according to their metabolite contents. Altogether, we determine their flavonoids/phenolics to evaluate their nutritive quality for the breeding program with the target of determining strong health-promoting compounds. The "Kalina" cultivar is highlighted as the best one with the highest number of metabolites, containing 14 out of the 26 evaluated; it was selected as the highest-quality cultivar, compared with the other seven cultivars studied. According to the grouping of the cultivars in principal component analysis PCA, their positive distribution is explained mainly by them having the highest contents of aminobutyric and isocitric acids, methionine and alanine and lower levels of fumaric acid, pyroglutamic acid and glycine, in contrast to the cultivars distributed negatively, which had high contents of carbohydrates and relatively low contents of most of the amino acids. The highest number of amino acids was found in the cultivar "Kalina", followed by "Perun" and "Bor". The highest number of carbohydrates was found in "Pavelsko" and "Iverce", while the prominent accumulation of organic acids was found in "Kalina", "Bor" and "Rozhen". The highest number of flavonoids in the flesh of the tubers was found in the cultivars "Nadezhda" and "Pavelsko", followed by "Bor". The highest ratio of flavonoids/phenolics in the flesh was found in "Pavelsko" and in "Nadezhda", followed by "Iverce". [ABSTRACT FROM AUTHOR]

Summary: The effect of microwave treatment on phenolics, flavonoids, antioxidant capacity, and phenolic in vitro bioaccessibility of white and red sorghum was evaluated. After microwave treatment for 40 s, the contents of free, bound, and total phenolics in white and red sorghum increased by 15.6–42.6, 4.0–23.4, and 19.7–66.0 mg gallic acid equivalents 100 g−1 dry weight (DW), respectively, and the contents of free, bound, and total flavonoids increased by 9.4–11.2, 4.8–5.4, and 14.2–16.6 mg rutin equivalents 100 g−1 DW, respectively. With increasing microwave treatment time, the contents of phenolic acids and flavonoids in white and red sorghum first increased and then decreased. Additionally, the changes in antioxidant capacity indicated a similar trend with phenolic contents for white and red sorghum. In vitro gastrointestinal digestion indicated that microwave treatment had no effect on the phenolic bioaccessibility of white and red sorghum but increased the release amount of phenolic acid and antioxidant capacity. [ABSTRACT FROM AUTHOR]

Insufficient forage supply from natural rangelands during the dry season hampers ruminants' performance in the derived savanna zone of Nigeria. Therefore, to provide alternative feed resources, we evaluated silage potential of Columbus grass (Sorghum almum Parodi) at boot, dough and maturity stages for 4‐, 8‐, 12‐ and 16‐week ensiling durations, in a 3 × 4 factorial experiment. The dry matter yield (DMY) was determined at each harvest stage, and the forages were subsequently ensiled. The results revealed that the DMY of S. almum at maturity was 8.98% and 18.67% higher than at dough and boot stages, respectively (p < 0.05). The silages were ranked "good silage" based on physical characteristics. Silage pH decreased with advancing ensiling duration from 4 to 16 weeks in boot (5.25–4.63), dough (5.63–4.11) and maturity (5.42–4.91), with a corresponding increase in lactic and volatile fatty acids. Crude protein (CP) was higher in forages ensiled at maturity and increased with ensiling duration after initial decline from 4 to 8 weeks. Neutral detergent fiber (NDF) and hemicellulose increased quadratically from boot to dough and subsequently declined at maturity. Growth stage, ensiling duration and their interactions influenced the macrominerals' content (p < 0.05). Silages' dry matter intake (DMI) declined from boot to dough stage across the ensiling durations, and except for 8 weeks, the DMI increased at maturity by 6.71%, 5.54% and 7.19% in 4, 12 and 16 weeks, respectively. Therefore, owing to the lower NDF and high DMY, CP and DMI of S. almum ensiled at maturity for 16 weeks, it could guarantee a quantitative and qualitative forage supply to maintain optimum animal performance throughout the dry season. [ABSTRACT FROM AUTHOR]

This study was aimed to evaluate the effect of sugarcane molasses on the silage quality of sorghum-sudangrass hybrid (Sorghum bicolor x Sorghum sudanense). To explore this objective Sorghum-sudangrass hybrid was harvested at the milking stage and well-chopped for making silage in the laboratory silos by using sugarcane molasses at the rate of 0, 1, 2, and 3%. Silage developed so, was analyzed for nutritive quality traits (dry matter, pH, crude protein, neutral detergent fiber, acid detergent fiber, cellulose, hemicelluloses and ash). Silage quality was also assessed by calculating digestible dry matter, dry matter intake, digestible energy, metabolizable energy and relative feed value. Flieg score was deliberated by using the pH and dry matter. The results of the research revealed that application of molasses improved dry matter (26.27%), crude protein (8.14) and ash contents (7.68) as compared to the control, but had lower values of pH (3.99), neutral detergent fiber (57.86), acid detergent fiber (30.74), lignin (3.72), cellulose (27.02) and hemicelluloses (27.13) in comparison with the untreated silage (control). Flieg score of treated silage was also in the category of very good. All these findings lead us to conclude that the silage quality of sorghum-sudangrass can be considerably improved by the use of sugarcane molasses as an additive. Molasses addition at a rate of 3% can improve the nutritive value of sorghum-sudangrass silage to the maximum extent. [ABSTRACT FROM AUTHOR]

Potatoes (Solanum tuberosum L.) are the fourth most economically important crop in the world. They have a short period of vegetation and are an excellent source of carbohydrates, amino acids, vitamins, organic acids, minerals and phenolics as antioxidant substances. Potato can be a major dietary source of various bioactive compounds. In this study, we applied gas chromatography coupled with mass spectrometry (GC-MS) metabolite profiling to classify eight Bulgarian potato cultivars bred in the Maritsa Vegetable Crops Research Institute (VCRI), Plovdiv, according to their metabolite contents. Altogether, we determine their flavonoids/phenolics to evaluate their nutritive quality for the breeding program with the target of determining strong health-promoting compounds. The “Kalina” cultivar is highlighted as the best one with the highest number of metabolites, containing 14 out of the 26 evaluated; it was selected as the highest-quality cultivar, compared with the other seven cultivars studied. According to the grouping of the cultivars in principal component analysis PCA, their positive distribution is explained mainly by them having the highest contents of aminobutyric and isocitric acids, methionine and alanine and lower levels of fumaric acid, pyroglutamic acid and glycine, in contrast to the cultivars distributed negatively, which had high contents of carbohydrates and relatively low contents of most of the amino acids. The highest number of amino acids was found in the cultivar “Kalina”, followed by “Perun” and “Bor”. The highest number of carbohydrates was found in “Pavelsko” and “Iverce”, while the prominent accumulation of organic acids was found in “Kalina”, “Bor” and “Rozhen”. The highest number of flavonoids in the flesh of the tubers was found in the cultivars “Nadezhda” and “Pavelsko”, followed by “Bor”. The highest ratio of flavonoids/phenolics in the flesh was found in “Pavelsko” and in “Nadezhda”, followed by “Iverce”.

The quality of potatoes intended for consumption or food processing depends primarily on their variety and chemical composition, which can be modified by growing conditions, place of cultivation, type of soil, amount of rainfall, as well as fertilization and plant protection products. This study aims to investigate the content of biologically active compounds in new varieties of potatoes with purple and yellow flesh, harvested in different cultivation locations. The content of the basic chemical composition and biologically active compounds in the raw material depended primarily on their variety, and to a lesser extent on the place of cultivation. Higher levels of kynurenic acid (KYNA), phenolic acids, and amino acids were found in potatoes of the new variety with purple-fleshed Provita compared to potatoes with yellow-fleshed Ismena. Potatoes with purple-flesh were characterized by over 40% lower amount of total glycoalcaloids (TGA). Most of the determined biologically active compounds, i.e. anthocyanins, phenolic acids, KYNA, and TGA varied depending on the place of origin of the potatoes, at the level of 40–66%. The biggest differences were found in the content of flavonols and amino acids, in the case of these substances the differences between potatoes from different places of cultivation ranged from 88% to 99%. • Higher levels of biologically active compounds were in potatoes with purple-fleshed. • Potatoes with purple-flesh were characterized by over 40% lower amount of TGA. • Most of biologically active compounds varied depending on the place of potatoes' origin. • Potatoes grown on light and strong clay sand soils had more biologically active compounds. [ABSTRACT FROM AUTHOR]

Staple foods produced from composite flour are considered feasible to alleviate protein-energy malnutrition (PEM). However, one of the major limitations of composite flour is poor protein digestibility. The biotransformation process mediated by probiotics via solid-state fermentation (SSF) holds a promising potential to address the poor protein digestibility in composite flour. Yet, there is no report established in this regard to the best of our knowledge. Therefore, 4 strains of Lactiplantibacillus plantarum and Pediococcus pentosaceus UP2 isolated from Malaysian foods that were previously reported to produce versatile extracellular hydrolytic enzymes were employed to biotransform gluten-free composite flour derived from rice, sorghum, and soybean. The SSF process was performed under 30-60% (v/w) moisture content for 7 days, where samples were withdrawn at 24 h intervals for various analyses such as pH, total titratable acidity (TTA), extracellular protease activity, soluble protein concentration, crude protein content, and in vitro protein digestibility. The pH of the biotransformed composite flour showed a significant reduction from the initial range of pH 5.98-6.67 to the final pH of 4.36-3.65, corresponding to the increase in the percentage of TTA in the range of 0.28-0.47% to 1.07-1.65% from days 0 to 4 and remained stable till day 7 of the SSF process. The probiotics strains exhibited high extracellular proteolytic activity (0.63-1.35 U/mg to 4.21-5.13 U/mg) from days 0 to 7. In addition, the treated composite flour soluble protein increased significantly ( p ≤ 0.05) (0.58-0.60 mg/mL to 0.72-0.79 mg/mL) from days 0 to 7, crude protein content (12.00-12.18% to 13.04-14.39%) and protein digestibility (70.05-70.72% to 78.46-79.95%) from days 0 to 4 of SSF. The results of biotransformation of 50% (v/w) moisture content were mostly comparable to 60% (v/w) moisture content, implying 50% (v/w) moisture content was the most suitable moisture content for the effective biotransformation of gluten-free composite flour mediated by probiotics via SSF since flour quality is better at lower moisture content. As for the overall performance, L. plantarum RS5 was ranked the best strain, attributed to the general improvement in the physicochemical properties of composite flour., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2023 Koyum, Foo, Ramli and Loh.)

Mussels have a particular nutritional value, representing a highly valued food source and thus sought after worldwide. Their meat is a real culinary delicacy, rich in proteins, lipids, carbohydrates, trace elements, enzymes, and vitamins. The seasonal variation of mussels' biochemical composition has been studied to determine the best harvesting period to capitalize on various biologically active fractions. In this work biochemical determinations have been performed on fresh flesh samples of Mytilus galloprovincialis specimens from the Black Sea coast to study seasonal variations in mussels' biochemical compounds. An analysis of significant lipid classes and the fatty acid composition of lipid extracts obtained from mussel flesh has also been performed. Since mussels retain pollutants from the marine environment, in parallel, the concentration of heavy metals in the meat of mussels collected for the analysis of the chemical composition was investigated. The impact and risk of heavy metal poisoning due to food consumption of mussels contaminated due to pollution of the marine harvesting area was evaluated by the bio-concentration factor of metals and estimated daily intakes of heavy metals through mussel consumption. [ABSTRACT FROM AUTHOR]

• Conventional perception about benefits of light walnut kernels was challenged. • Relationships between color or texture and intrinsic qualities were relatively low. • Kernels with a light color and a simple texture have better comprehensive qualities. The intrinsic nutritive components (fatty acids, amino acids, tocopherols and mineral elements) of kernels collected from the main planting area of China with varying appearance (color and texture) were assessed. The significant (p < 0.05) differences between different color groups were primarily for γ-tocopherol, K and Na, with contents ranging from 246.48 to 333.94 mg/kg, 3403.05 to 4409.65 mg/kg and 12.62 to 22.16 mg/kg, respectively. There was a significant (p < 0.05) difference between two texture groups only for γ-tocopherol, with the content of 303.04 and 342.08 mg/kg, respectively. Further investigation revealed that kernels with a light color and a simple texture had a significant (p < 0.05) advantage in terms of comprehensive qualities, particularly in the contents of total amino acids (191.91 mg/kg) and elements (P: 4165.57 mg/kg, Mn: 75.34 mg/kg). Furthermore, the advantageous kernels' high variability in monounsaturated fatty acids (25.7 %) suggests that they could be a good resource for breeding programs. [ABSTRACT FROM AUTHOR]