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A significant proportion of patients with peripheral artery disease (PAD) displays a poor response to aspirin and/or the platelet P2Y 12 receptor antagonist clopidogrel. This phenomenon is reflected by high on-treatment platelet reactivity (HTPR) in platelet function assays in vitro and is associated with an increased risk of adverse cardiovascular events. This study aimed to elucidate specific plasma protein signatures associated with HTPR to aspirin and clopidogrel in PAD patients. Based on targeted plasma proteomics, 184 proteins from two cardiovascular Olink panels were measured in 105 PAD patients. VerifyNow ASPI- and P2Y 12 -test values were transformed to a continuous variable representing HTPR as a spectrum instead of cut-off level-defined HTPR. Using the Boruta random forest algorithm, the importance of 3 plasma proteins for HTPR in the aspirin, six in clopidogrel and 10 in the pooled group (clopidogrel or aspirin) was confirmed. Network analysis demonstrated clusters with CD84, SLAMF7, IL1RN and THBD for clopidogrel and with F2R, SELPLG, HAVCR1, THBD, PECAM1 , TNFRSF10B, MERTK and ADM for the pooled group. F2R, TNFRSF10B and ADM were higher expressed in Fontaine III patients compared to Fontaine II, suggesting their relation with PAD severity. A plasma protein signature, including eight targets involved in proatherogenic dysfunction of blood cell-vasculature interaction, coagulation and cell death, is associated with HTPR (aspirin and/or clopidogrel) in PAD. This may serve as important systems-based determinants of poor platelet responsiveness to aspirin and/or clopidogrel in PAD and other cardiovascular diseases and may contribute to identify novel treatment strategies. [Display omitted] • A spectrum of high on-treatment platelet reactivity (HTPR) was established for PAD. • HTPR-associated plasma protein signatures differ between aspirin and clopidogrel. • Pooled HTPR relates to PSGL1, PECAM1, TM, PAR1, TRAILR2, ADM, KIM1 and MERTK. • Higher plasma levels of PAR1, TRAILR2 and ADM are associated with PAD severity. • Systems-based plasma protein signatures may determine antiplatelet therapy response. [ABSTRACT FROM AUTHOR]

Residual venous obstruction (RVO) after deep vein thrombosis (DVT) is considered a risk factor of recurrent venous thromboembolism (VTE), arterial events and post-thrombotic syndrome (PTS). We hypothesized thrombo-inflammatory markers might be associated with RVO and clinical outcomes. In a DVT cohort with routine RVO-assessment and 5-year follow-up, patients were invited for blood withdrawal after stopping anticoagulants. Thrombin generation potential, coagulation enzyme:inhibitor complexes, soluble platelet markers and clinical markers were measured in platelet-poor plasma. Associations were represented as odds ratio (OR) or hazard ratio (HR) per standard deviation. Patients with RVO (102/306, 33 %) had higher rates of PTS (24 vs. 12 %, p = 0.008), but similar rates of recurrence (16 vs. 15 %, p = 0.91) and arterial events (7 vs. 4 %, p = 0.26). RVO was associated with thrombin peak height (OR 1.40 [1.04–1.88]), endogenous thrombin potential (ETP, OR 1.35 [1.02–1.79]), and CRP (OR 1.74 [1.10–2.75]). Recurrent VTE was associated with ETP (HR 1.36 [1.03–1.81]), FXIa:C 1 -inhibitor (HR 1.34 [1.04–1.72]), thrombin:antithrombin (HR 1.36 [1.16–1.59]), soluble P-selectin (HR 2.30 [1.69–3.11]), soluble glycoprotein VI (sGPVI, HR 1.30 [1.01–1.69]), D-dimer (HR 1.56 [1.31–1.86]), and factor VIII (HR 1.44 [1.15–1.82]). Arterial events were associated with sGPVI (HR 1.80 [1.25–2.59]). PTS was not associated with any marker. Our findings indicate RVO was associated with thrombo-inflammation, but this did not predict clinical outcomes in this setting. Importantly, we found recurrent VTE was associated with ongoing coagulation and platelet activation in patients well beyond the acute phase of DVT. Furthermore, sGPVI indicated an increased risk of arterial events, highlighting the role of platelets in arterial thrombosis following DVT. [Display omitted] • RVO was associated with TG and CRP, but not with active thrombo-inflammation. • Activated factor XI and P-selectin levels were predictive of recurrent VTE. • GPVI levels were predictive of both venous and arterial thrombotic events. [ABSTRACT FROM AUTHOR]