1. Rev-erbα regulates circadian rhythms and StAR expression in rat granulosa cells as identified by the agonist GSK4112
- Author
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Chen, Huatao, Chu, Guiyan, Zhao, Lijia, Yamauchi, Nobuhiko, Shigeyoshi, Yasufumi, Hashimoto, Seiichi, and Hattori, Masa-aki
- Subjects
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CIRCADIAN rhythms , *GENE expression , *GENETIC regulation , *TRANSCRIPTION factors , *METABOLISM , *BIOSYNTHESIS , *LABORATORY rats - Abstract
Abstract: The Rev-erbα gene is regarded as a circadian clock gene and clock-regulated gene which regulates the circadian transcriptional/translational loop in a subtle way. Here, we first detected the circadian oscillation in mature granulosa cells from antral follicles using a real-time monitoring system of Per2 promoter activity with the addition of FSH. Then we used GSK4112, an agonist ligand of Rev-erbα, to investigate the function of Rev-erbα. GSK4112 treatment significantly reduced the Per2-dLuc amplitude and induced the Per2 oscillation phase advance shift. GSK4112 significantly inhibited Bmal1 mRNA expression, whereas it did clearly stimulate expression of StAR mRNA in a dose-dependent manner. Our data are the first to show the Rev-erbα function in the steroid biosynthesis of rat granulosa cells, and to suggest that Rev-erbα may coordinate circadian rhythm and metabolism in rat ovaries. [Copyright &y& Elsevier]
- Published
- 2012
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