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A novel fluorescent BODIPY-rhodamine dyad probe that operates based on through bond energy transfer (TBET) phenomenon has been synthesized and its application in the detection of biologically relevant metal ions is explored. Among several metal ions, the probe selectively responds to the addition of trivalent metal ions (Al 3+ , Cr 3+ and Fe 3+ ) through its signature absorbance and fluorescence characteristics and permits their ratiometric detection. The trivalent metal ion detection ability of the probe is unaffected with the presence of other commonly coexistent ions. The probe is highly sensitive and it can detect the trivalent metal ions even at ppb level concentrations. The probe is nontoxic under the experimental conditions, cell permeable and useful for the imaging of Al 3+ , Cr 3+ and Fe 3+ ions present in W138 (normal lung fibroblast) cells. [ABSTRACT FROM AUTHOR]

A novel fluorescein-rhodamine dyad probe has been synthesized and its chemosensory applications are explored. Anion and cation specific responses of the probe permit ratiometric detection of Hg 2+ and fluoride ions, individually and collectively, with different fluorescence outputs. The presence of other commonly coexistent ions does not affect the Hg 2+ and fluoride ion detection ability of the probe. The probe is highly sensitive to Hg 2+ and fluoride ions and it can detect Hg 2+ and fluoride ions even at ppb level concentrations. The probe is non-toxic under the experimental conditions, cell permeable and useful for the imaging of intracellular Hg 2+ and fluoride ions. [ABSTRACT FROM AUTHOR]

A rhodamine-based optical sensor Rh–3S has been developed for selective detection of Hg 2+ in aqueous solution as well as in living cells. UV–vis and fluorescence spectroscopic studies reveal that Rh–3S shows marked sensitivity and selectivity to Hg 2+ with a chelation-induced “off–on” response in acetonitrile (ACN)/MOPS buffer (10 mM, pH 7.3, v/v, 1:1). The Rh–3S sensor binds Hg 2+ in a 1:1 stoichiometry with an apparent binding constant 3.71 × 10 6 M − 1 (log K = 6.57) and displays a distinct change in color and fluorescence upon the alteration of free Hg 2+ levels in solution with a reversible response and little interference with other biological relevant metal ions. Live cell confocal imaging studies demonstrate that the sensor is also capable of imaging the presence of Hg 2+ ions as well as its dynamic changes in live cells. [ABSTRACT FROM AUTHOR]