1. 78-P: DIFFERENTIAL TRANSCRIPTION PROFILES FOR MHC CLASS I GENES IN ORANGUTANS: NEGLIGIBLE TRANSCRIPTION OF C AND PARTICULAR B LOCUS/LINEAGE IN PERIPHERAL BLOOD DERIVED CELL-LINES.
- Author
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de Groot, Natasja G., Heijmans, Corrine M.C., van der Wiel, Marit K., Blokhuis, Jeroen H., Mulder, Arend, Guethlein, Lisbeth A., Doxiadis, Gaby G.M., Claas, Frans H., Parham, Peter, and Bontrop, Ronald E.
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GENETIC transcription , *MAJOR histocompatibility complex , *ORANGUTANS , *LOCUS (Genetics) , *PONGO abelii , *HAPLOTYPES , *ANTISENSE DNA , *NUCLEOTIDE sequence - Abstract
Aim: Humans and orangutans shared a common ancestor about 12-16 million years ago. Two orangutan species are officially recognized; one inhabiting Borneo (Pongo pygmaeus) and the other Sumatra (Pongo abelii). Orangutans possess A, B, and C genes that are homologous to their HLA equivalents. Unlike humans where each of these is found as a single copy gene on a haplotype, orangutan haplotypes may possess a duplicated B gene whereas others lack C. In theory, orangutans may express from 3 to 8 different classical MHC class I allotypes. Conventional sequencing of cDNA derived from six different orangutan samples, using 4 different primer sets, suggested that certain alleles, in particular MHC-C, are less well transcribed. Therefor we aimed to investigate the transcription/expression profile of the orangutan MHC class I genes. Methods: cDNA, derived from five different orangutan EBV transformed B-cell-lines (two Popy and three Poab), was used as starting material. Generic primers were designed, and relevant amplicons were sequenced in both directions using massively parallel sequencing. Results: The analyses showed that the A locus is most abundantly transcribed. The B locus exhibits differential transcription patterns that seem to be dependent on the B allotype present in an animal. The present analyses show an almost negligible transcription of the B∗03-locus/lineage. In addition, the C locus, if present, appears to be minutely transcribed. Moreover, the cell-surface expression of several MHC allotypes was assessed using various monoclonal antibodies. Conclusions: The present data suggest a minor immunological role for B∗03-lineage and C-locus molecules in orangutangs as they appear to be virtually absent on peripheral blood derived cells. Besides, all animals investigated transcribe at least one variant of the B∗08-lineage that encodes the C1-epitope, the ligand for particular killer cell Ig-like receptor, and most likely compensate for the partial loss of the C-locus in orangutans. [Copyright &y& Elsevier]
- Published
- 2013
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