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Detection and conversion of mechanical forces into biochemical signals is known as mechanotransduction. From cells to tissues, mechanotransduction regulates migration, proliferation, and differentiation in processes such as immune responses, development, and cancer progression. Mechanosensitive structures such as integrin adhesions, the actin cortex, ion channels, caveolae, and the nucleus sense and transmit forces. In vitro approaches showed that mechanosensing is based on force-dependent protein deformations and reorganizations. However, the mechanisms in cells remained unclear since cell imaging techniques lacked molecular resolution. Thanks to recent developments in super-resolution microscopy (SRM) and molecular force sensors, it is possible to obtain molecular insight of mechanosensing in live cells. We discuss how understanding of molecular mechanotransduction was revolutionized by these innovative approaches, focusing on integrin adhesions, actin structures, and the plasma membrane. Mechanotransduction is involved in a variety of cell and tissue processes. For this, mechanosensitive structures, such as integrin adhesions, the cytoskeleton, or the plasma membrane, are key to sense, integrate, and transmit mechanical forces. Novel imaging methods and molecular sensors have unveiled how molecular mechanosensing occurs in these structures within the context of live cells. These include super-resolution imaging, single particle tracking, and molecular force sensors. Super-resolution microscopy and single protein tracking have revealed the 3D nanoscale organization and dynamics of integrin adhesion structures in various cell types, which are divided into functional nanolayers with specific paths for protein diffusion. Forces on actin regulators and F-actin trigger conformational changes controlling actin regulator function and binding, and therefore F-actin assembly and architecture. In migrating cells, mechanical plasticity emerges from global geometrical reorganization of actin networks under loads, or forces from elongating actin filaments controlling locally actin regulators dynamics and functions. Cell surface mechanics are emerging as a key parameter controlling cell motility, division, and differentiation. Variations in cortical tension and membrane-to-cortex attachment regulate the timing of differentiation while the nanoscale architecture of the actin cortex is linked to cortical tension. [ABSTRACT FROM AUTHOR]

The biomechanical regulation of the cytoskeleton and cell adhesions underlies various essential cellular functions. Studying them requires visualizing their nanostructure and molecular dynamics with evermore precise spatio-temporal resolution. In this review we will focus on the recent advances in single molecule fluorescence imaging techniques and discuss how they improve our understanding of mechanically sensitive cellular structures such as adhesions and the cytoskeleton. We will also discuss future directions for research, emphasizing on the 3D nature of cellular structures and tissues, their mechanical regulation at the molecule level, as well as how super-resolution microscopy will enhance our knowledge on protein structure and conformational changes in the cellular context. [ABSTRACT FROM AUTHOR]

As a promising method for local tumor treatment, nanosecond pulsed electric field (nsPEF) ablation elicits a potent anti-tumor immune response. However, the mechanism of the nsPEF-mediated anti-tumor immune response and its effects on the tumor microenvironment remains unclear. Here, we demonstrated that nsPEF treatment increased the level of membrane PD-L1 in liver cancer cells. Furthermore, nsPEF induced the release of PD-L1-associated extra-cellular vesicles, leading to the dysfunction of CD8+ T cells, which could potentially be reversed by PD-L1 blockade. Biological and functional assays also demonstrated that nsPEF treatment resulted in the increased PD-L1 level and dysfunction of infiltrated CD8+ T cells in tumor tissues in vivo, indicating the long term antitumor efficacy of nsPEF treatment. A combination of nsPEF treatment and PD-L1 blockade effectively inhibited tumor growth and improved the survival of the tumor-bearing mouse. In conclusion, nsPEF treatment induced the translocation and release of PD-L1 and contributed to the dysfunction of infiltrated CD8+ T cells, resulting in tumor progression at later stages. The combination of nsPEF treatment and PD-L1 blockade is a promising therapeutic strategy for liver cancer. [ABSTRACT FROM AUTHOR]

The LiNi 0.5−x Zn x Mn 1.48 Y 0.02 O 4 (x = 0.01, 0.03, 0.05) series were prepared via a sol-gel method. The mechanism of the Zn2+ and Y3+ co-doping on the structural morphology and Mn3+ ion dissolution, which led to the improved electrochemical performance of the LiNi 0.5 Mn 1.5 O 4 (LNMO) sample, were studied. Compared with the pristine LNMO, Zn-Y co-doping led to increased lattice parameters, morphological evolution, decreased Mn3+ content, and better electrochemical properties. Among all the samples, LiNi 0.47 Zn 0.03 Mn 1.48 Y 0.02 O 4 (0.03ZnY) showed optimal electrochemical properties at room temperature and elevated temperature. After 200 cycles, the 0.03ZnY sample showed excellent cycling performance, i.e., 134.529 mAh g−1 at 1 C (with a capacity retention of 94.7%), which was higher than the pristine sample (104.913 mAh g−1, with a capacity retention of 92.7%). Furthermore, when tested at 1 C, at a high temperature (55 °C), the 2.03Zn-Y sample reached a discharge capacity of 94.004 mAh g−1 and maintained a 79.6% capacity retention after 120 cycles, which was higher than the pristine sample (52.430 mAh g−1, 43.6% capacity retention). • The pristine LNMO and Zn-Y co-doping cathode materials was synthesized via sol-gel method, co-doping did not change the structure of materials, and the lattice parameter of materials increased, enhancing the electrochemical performance. • LiNi 0.47 Zn 0.03 Mn 1.48 Y 0.02 O 4 (0.03ZnY) process the best electrochemical performance, which obtain 134.529 mAh/g at 1 C after 100 cycles at 25 ℃, higher than that of pristine sample(104.913 mAh/g). • Moreover, 0.03ZnY process 94.004 mAh/g at high temperature(55 ℃) at 1 C for 120 cycles, much higher than pristine sample(52.430 mAh/g). [ABSTRACT FROM AUTHOR]

The closure of gaps is crucial to maintaining epithelium integrity during developmental and repair processes such as dorsal closure and wound healing. Depending on biochemical as well as physical properties of the microenvironment, gap closure occurs through assembly of multicellular actin-based contractile cables and/or protrusive activity of cells lining the gap. This review discusses the relative contributions of ‘purse-string’ and cell crawling mechanisms regulated by cell–substrate and cell–cell interactions, cellular mechanics and physical constraints from the environment. [ABSTRACT FROM AUTHOR]

Superhydrophobic nano Cu/Al 2 O 3 Ni–Cr composited coating with a low adhesive force was deposited onto the Q345 carbon steel via electro-brush plating. Surface morphologies of nano Cu/Al 2 O 3 Ni–Cr composited coating were investigated by scanning electron microscope (SEM). Chemical compositions were characterized by energy dispersive spectroscopy (EDS). First of all, by adjusting different process parameters such as working voltage, relative velocity, Cu particles concentration and plating time, we obtain the most optimal parameters: working voltage is 15 V, relative velocity is 4.8 m/min, Cu particles concentration is 5 g/L and plating time is 60 s. Under the best process parameters, the water contact angle reaches to 156° and a sliding angle is less than 2° on the nano Cu/Al 2 O 3 Ni–Cr coating. Then the mechanism of the superhydrophobic and low adhesion characteristic of this surface were explained by Cassie's model. Low adhesive force can be characterized by max rebound height of water droplet. As a result, to achieve low adhesive surface it is necessary to decrease the fraction of the solid/liquid interface under the water droplet. Finally the coating was proved to have an excellent self-cleaning performance. [ABSTRACT FROM AUTHOR]

[Display omitted] • Superhydrophobic PLA surface can be prepared by a one-step spraying methods. • The as-prepared PLA fabric can be used for continuous oil-water separation. • The as-prepared PLA fabric can separate oil-water mixtures under harsh environments. • The as-prepared PLA fabric exhibits outstanding environmental and mechanical stability. Various types of functional materials incorporating superhydrophobic components have been rapidly developed for efficient oil/water separation. The post-processing of these used separated materials remains a challenging problem due to their non-biodegradable characteristics in natural environments, thus easily contributing to secondary contamination. Herein, we report a biodegradable superhydrophobic PLA fabric with hierarchical coral-like micro/nano structures by a one-step spraying method. The as-prepared PLA fabric was found to be both superhydrophobic and superoleophilic with a static water contact angle of 161 ± 2° and an oil contact angle of 0°. In addition, the as-prepared PLA nonwoven fabrics also revealed efficient continuous oil separation efficiency (>97.5%) and tiny water droplet collection efficiency, as well as high oil flux ranging from 2239 L·m−2·h−1 to 59,713 L·m−2·h−1. Furthermore, these fabrics retained their inherent wetting property and oil separation ability in a variety of acidic and basic solutions, 3.5 wt% NaCl solution, hot (100 °C), and freezing (0 °C) temperatures. This study provides a novel and environmentally friendly method for preparing biodegradable oil–water separation materials with green post-treatment characteristics when compared to the non-biodegradable separation materials, indicating potential applications in the fields of green water treatment and petroleum refining. [ABSTRACT FROM AUTHOR]

The CD47–signal regulatory protein α (SIRPα) axis inhibits dendritic cell (DC) phagocytosis and contributes to immune evasion. However, the behaviour of DCs and the potential crosstalk between DCs and natural killer (NK) cells in the hepatocellular carcinoma (HCC) microenvironment after CD47 blockade remain unclear. The infiltration of CD103+ DCs and NK cells were analysed by immunohistochemistry and immunofluorescence in both human and murine HCC specimens. An orthotopic liver tumour model was used to evaluate the function of the CD103+ DC–NK cell axis after CD47 blockade in vivo in wild-type, Rag1 -/- , Batf3 -/- , and STING1 -/- mice. Phagocytosis assays were performed in CD103+ DC and HCC cell lines. CD103+ DC-derived cytokines were analysed by chemokine array. Spleen-derived NK cells in C57BL/6J mice were used to evaluate cytotoxic functions in vitro. Higher CD47 expression was associated with worse prognosis in patients with HCC. CD47 blockade enhanced antitumour efficacy by stimulating the CD103+ DC–NK cell axis. The hypoxic microenvironment promoted CD47 blockade-induced tumour DNA phagocytosis by CD103+ DCs. By releasing IL-12 and CXCL9, activated CD103+ DCs induced the recruitment of NK cells with upregulated expression of granzyme B, NKG2D, interferon-γ, and tumour necrosis factor-α and downregulated expression of NKG2A. The antitumour effects of CD47 blockade could be abolished by cyclic GMP–AMP synthase (cGAS)–STING pathway inhibition. In addition to the classical DC–T cell axis, CD47 blockade significantly enhanced the ability of CD103+ DCs to take up tumour DNA, resulting in the stimulation of the cGAS–STING pathway, which promoted the infiltration and activation of NK cells in liver cancer. Hypoxia (low oxygen levels) is prevalent in the hepatocellular carcinoma microenvironment and promotes the phagocytosis (ingestion and elimination) of tumour DNA by CD103+ dendritic cells (a type of immune cell). Blockade of the cell surface protein CD47 resulted in activation of CD103+ dendritic cells which led to the recruitment and activation of natural killer cells (a different immune cell). When activated, these cells exhibit an antitumour effect. [Display omitted] • CD47-blockade enhances antitumour efficacy by triggering the CD103+ DC–NK cell axis in HCC. • CD47 blockade promotes CD103+ DC phagocytosis in synergy with hypoxia. • CD103+ DC-derived IL-12 and CXCL9 are responsible for NK cell activation and recruitment. • The intrinsic activation of the cGAS–STING pathway of CD103+ DC facilitates NK cell activation and recruitment in CD47 blockade. [ABSTRACT FROM AUTHOR]

[Display omitted] • It established a cement-prosthesis stem debonding experiment to analyze the initial damage process of the interfacial adhesion and explored the relationship between the inter-facial adhesion stress and slip. • In terms of nondestructive testing technology detect damage of stem surface and inner bone cement, micro-morphology and micro-pores and micro-cracks and annular damage belts have been assessed and analyzed. The initial interface debonding damage behavior of cemented hip arthroplasty was simulated and investigated through the push-in experiment. A series of nondestructive testing techniques were carried out to detect damage to the stem-cement interface and cement. Results suggested that the initial stem-cement interface debonding and cement damage were induced at femur loading early period. The cement was subjected to a combination action of shear stress and bending from a sinking stem causing a pressure-arch effect resulting in compression creep and micro-crack on the cement successively. The stem-cement interfacial friction prevent stem from sinking due to the internal shear effect caused by the cement-stem interface micro-sliding. The interface debonding procession increased at first and then became stable, which was shown in cement's creep deformation-friction-fracture model. The initial micro-cracks released damage energy which was converted into stress waves. Further, the relationship of bond stress and slippage at different locations caused different degrees of damage belt for the interface and cement. It was concluded that the combination effect of pressure arching and shear lag was the primary reason for cement's initial damage and the matrix defect affected the failure progress. [ABSTRACT FROM AUTHOR]

Superhydrophobic surface is of wide application in the field of catalysis, lubrication, waterproof, biomedical materials, etc. The superhydrophobic surface based on hard metal is worth further study due to its advantages of high strength and wear resistance. This paper investigates the fabrication techniques towards superhydrophobic surface on carbon steel substrate via electric corrosion and studies the properties of as-prepared superhydrophobic surface. The hydrophobic properties were characterized by a water sliding angle (SA) and a water contact angle (CA) measured by the Surface tension instrument. A Scanning electron microscope was used to analyze the structure of the corrosion surface. The surface compositions were characterized by an Energy Dispersive Spectrum. The Electrochemical workstation was used to measure its anti-corrosion property. The anti-icing performance was characterized by a steam-freezing test in Environmental testing chamber. The SiC sandpaper and 500 g weight were used to test the friction property. The research result shows that the superhydrophobic surface can be successfully fabricated by electrocorrosion on carbon steel substrate under appropriate process; the contact angle of the as-prepared superhydrophobic surface can be up to 152 ± 0.5°, and the sliding angle is 1–2°; its anti-corrosion property, anti-icing performance and the friction property all show an excellent level. This method provides the possibility of industrialization of superhydrophobic surface based on iron substrate as it can prepare massive superhydrophobic surface quickly. [ABSTRACT FROM AUTHOR]

CD103+ DC is crucial for antitumor immune response. As a promising local therapy on cancers, nanosecond pulsed electric field (nsPEF) has been widely reported to stimulate anti-tumor immune response, but the underlying relationship between intratumoral CD103+ DC and nsPEF treatment remains enigmatic. Here, we focused on the behavior of CD103+ DC in response to nsPEF treatment and explored the underlying mechanism. We found that the nsPEF treatment led to the activation and accumulation of CD103+ DC in tumor. Depletion of CD103+ DC via Batf3−/− mice demonstrated CD103+ DC was necessary for intratumoral CD8+ T cell infiltration and activation in response to nsPEF treatment. Notably, NK cells recruited CD103+ DC into nsPEF-treated tumor through CCL5. Inflammatory array revealed CD103+ DC-derived IL-12 mediated the CCL5 secretion in NK cells. In addition, the boosted activation and infiltration of intratumoral CD103+ DC were abolished by cGAS-STING pathway inhibition, following IL-12 and CCL5 decreasing. Furthermore, nsPEF treatment promoting CD103+ DC-mediated antitumor response enhanced the effects of CD47 blockade strategy. Together, this study uncovers an unprecedented role for CD103+ DC in nsPEF treatment-elicited antitumor immune response and elucidates the underlying mechanisms. • CD103+ DC is necessary for antitumor immune response induced by nsPEF treatment. • NsPEF treatment leads to activation of intratumoral CD103+ DC. • NsPEF treatment induces the recruitment of CD103+ DC via IL12-NK-CCL5 axis. • CGAS-STING pathway controls activation and recruitment of CD103+ DC. • CD47 blockade promotes nsPEF-mediated activation and recruitment of CD103+ DC. [ABSTRACT FROM AUTHOR]

Inter-symbol interference (ISI), mode coupling (MC) and other impairments present in high baud rate mode division multiplexing (MDM) systems significantly degrade the reliability and accuracy of optical signal transmission. Therefore, performing joint multi-parameter optical performance monitoring (OPM) is crucial to ensure the efficient operation of the system. We propose a scheme that combines asynchronous single channel sampling (ASCS) images with a multi-task residual neural network (MT-ResNet) to enable cost-effective joint OPM at intermediate nodes in a 100 GBaud MDM system. The scheme requires only one photodetector to receive the signal. Based on the distinct characteristics exhibited by ASCS images for different parameters in the MDM system, the MT-ResNet is improved to capture correlations among parameters and learn residual information between inputs and outputs. This enables high-precision and rapid simultaneous implementation of modulation format identification (MFI), MC estimation, optical signal-to-noise ratio (OSNR) estimation, and chromatic dispersion identification (CDI) in the intermediate nodes of a 100 GBaud MDM system. The effectiveness of the proposed scheme is verified using a 100 GBaud QPSK/8QAM/16QAM/32QAM/64QAM MDM simulation transmission system with five spatial modes (LP 01 /LP 11a /LP 11b /LP 21a /LP 21b). The results show that under the influence of different linewidth (LW) of laser and differential mode group delay (DMGD), a 100 % identification success rate can be achieved for five commonly used MFs and eight different cumulative CD values in high baud rate MDM systems, across a wide range of OSNR. Additionally, the mean absolute error (MAE) of OSNR estimation corresponding to the five MFs is 0.22 dB, 0.26 dB, 0.30 dB, 0.32 dB, and 0.36 dB, respectively. The MAE of the eight different MC intensities fluctuate between 0.015 and 0.030. These results fully demonstrate the superior performance of the proposed scheme. Furthermore, the scheme exhibits favorable tolerance to large LWs and fiber nonlinear effects. Moreover, the complexity of the scheme is accurately analyzed through floating-point operations (FLOPs) and parameters (Params), which are much less than existing typical schemes, with specific values of 14.8849 M and 1.3798 M. • A novel joint OPM scheme by using ASCS images and MT-ResNet in 100 GBaud MDM system. • A low-cost architecture for performing OPM at intermediate nodes in MDM systems. • The scheme can realize MFI, CDI, OSNR and MC estimation at the same time. • The scheme exhibits favorable tolerance to large LWs and fiber nonlinear effects. • The scheme not only has low complexity but also exhibits excellent OPM performance. [ABSTRACT FROM AUTHOR]

• The coating cures quickly to obtain a superhydrophobic surface. • Prepared coating can be applied to a variety of complex surfaces. • The coating has good temperature resistance. • The coating has a certain degree of chemical and physical stability. Because of its significant role in the field of self-cleaning, super-hydrophobic coatings have attracted more and more attention from researchers around the world, while the properties of environmental weather resistance and structure-stability often limit the application in daily life of which. In this study, a fast curing super-hydrophobic coating with good thermal tolerance as well as structure-stability and can be applied to a variety of surfaces conveniently was fabricated with fluorocarbon resin (FEVE), methyl methacrylate (MMA) and micro/nanometer scale powders via solution method. A scanning electron microscope (SEM) and a confocal laser scanning microscope (CLSM) were used to evaluate the coating micro morphology. The influence of material content ratio of each component on wettability was analyzed by contact angle measuring instrument. The composition testing of Fourier Transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS) indicated that the coating has a high fluorine content. And the test of friction, constant temperature and immersion experiments showed appropriate physical/chemical stability and weather fastness of the coating. [ABSTRACT FROM AUTHOR]

Highlights • Synthesis of 3D dahlia-like Co 3 O 4 by hydrothermal deposition. • Co 3 O 4 coating has excellent superhydrophobicity after FAS-17 modification. • Unmodified Co 3 O 4 /nickel foam can efficiently separates various oil-water mixtures. • Superhydrophobic Co 3 O 4 /nickel foam can adsorb trichloromethane underwater. • Rapid oil-water separation and collection with different Co 3 O 4 /nickel foam. Abstract The synthesis of nanomaterial on porous materials and use of their porous structure for oil-water separation and oil absorption underwater at normal temperature and pressure are a very significant process, which has attracted wide attention. In this paper, dahlia-like Co 3 O 4 nanoparticles was synthesized on the surface of porous nickel foam by hydrothermal reaction. After modification with heptadecafluoro-1,1,2,2-tetrahydro-decyl-1-trimethoxysilane (FAS-17), the as-prepared sample surface could obtain good oleophobicity and excellent superhydrophobicity with high contact angles and low sliding angles. Furthermore, the as-prepared superhydrophobic composite had low adhesion to water and light oil, and could be subjected to simple and effective oil-water separation (including three light oil and one heavy oil) at normal temperature and pressure. Due to the magnetic properties of nickel foam, underwater oil absorption experiment was carried out by magnetic traction, which could achieve a perfect oil absorption effect. In addition, the comprehensive use of unmodified and modified Co 3 O 4 /nickel foam at the end of tube can quickly and efficiently carry heavy oil (trichloromethane)-water separation and collection. Therefore, the as-prepared dahila-like Co 3 O 4 on nickel foam not only has good application value in oil-water separation, but also has excellent potential in underwater oil absorption. [ABSTRACT FROM AUTHOR]

Abstract Tungsten trioxide (WO 3) and titanium dioxide (TiO 2) decorated commercial stainless steel meshes (SSM 304) were fabricated by chemical deposition and dip coating. WO 3 and TiO 2 particles were firmly and uniformly coated on the membrane surfaces, forming rough micro-nano double structures with underwater superoleophobicity, large contact angles and low oil-adhesion characteristic. Furthermore, the as-prepared membranes achieved effective oil-in-water (O/W) emulsions separation with high efficiency up to 98.5% or more. The separation of O/W emulsions was easily implemented under gravity with water fluxes of >200 L m−2 h−1 or low pressure with water fluxes up to 1300 L m−2 h−1 or more. In addition, the superhydrophilic membranes with WO 3 /TiO 2 coatings were demonstrated for high oil rejection, stable underwater superoleophobic properties after abrasion treatment and steady water permeation flux after several cycles. Highlights • A Method to prepare WO 3 /TiO 2 decorated steel meshes is proposed. • Membranes have a solid structure, good stability and underwater superoleophobicity. • Effectively membrane separation of stabilized oil-in-water emulsions. • Composite membranes have outstanding recyclability and potential for oil/water separation. [ABSTRACT FROM AUTHOR]

Fabrication of surfaces with capability of repelling both water and oil is badly needed. Meanwhile, the widespread use of nanomaterials has also attracted great interest in this field. In this paper, we provided novel methods to synthesize nano amorphous tungsten oxide coatings on Al-Mg metals. After modification with FAS-17, the surface could obtain excellent superamphiphobic abilities with lower sliding angles and higher contact angles to water and oil. In addition, outstanding anti-oil pollutant and low adhesion were obtained. Furthermore, the as-prepared superamphiphobic surfaces could survive 200 cm abrasion distance under 19.6 kPa pressure on 1000 grid SiC sandpaper. It should be noted that this coatings could obtain different colors in different temperature. Meanwhile, we found that the droplets with lower surface energy and low viscosity are easier to bounce than the droplets with high surface energy and high viscosity. So the simple procedure and excellent superamphiphobicity about fabricating colorful tungsten oxide coatings have great potential in protecting Al-Mg metal and have potential applied in signals which need to prevent wetting or pollution by water and oils. On one level, this method about synthesizing nano amorphous tungsten oxide also has more promising using in other fields. [ABSTRACT FROM AUTHOR]

The low-temperature mechanochemical devulcanization (LTMD) method can maintain the effectiveness of the original additives in waste rubbers after rubber reclamation. To confirm this discovery, the LTMD method and the traditional high-temperature atmospheric devulcanization (HTAD) method are used to reclaim waste rubber powders. The reclaimed rubbers are mixed with different amounts of additional additives and then revulcanized alone or revulcanized after blending with all-terrain vehicle (ATV) tread rubbers. The properties of the reclaimed rubbers and the revulcanized rubbers are characterized, and the results reveal that the original sulfur agent is inactive in both the LTMD and HTAD processes and that the original curing assistants maintain high effectiveness after the LTMD process but not after the HTAD process. The tensile strength of the revulcanized LTMD-reclaimed rubbers with no additional curing assistants reaches 97% of that of the revulcanized LTMD-reclaimed rubbers with full additional curing assistants, and 30% higher than that of the revulcanized HTAD-reclaimed rubbers with full additional curing assistants. When blended with 40% reclaimed rubber from ATV tread rubber, the tensile strength of the vulcanized LTMD blends with only the sulfur agent reaches 89% of the pristine ATV tread rubber while that of the vulcanized HTAD blends with full additives only reaches 76% of the pristine ATV tread rubber. The LTMD method not only achieves high-value recycling of waste rubbers but also avoids the failure of the original curing assistants in waste rubbers, thereby saving large amounts of materials and showing great application prospects. [Display omitted] • Low-temperature mechano-chemical devulcanization is used for rubber reclamation. • High-temperature of HTAD method leads to failure of original curing assistants. • LTMD method maintains the effectiveness of original curing assistants. • LTMD method obtains high performance of reclaimed rubbers. • LTMD method saves curing assistants for reuse of reclaimed rubbers. [ABSTRACT FROM AUTHOR]