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A combining of MIMO signal processing with OFDM is regarded as a promising solution of enhancing the performance of next generation wireless system. Therefore, in the paper, an OFDM-based wireless system employing layered space-time architecture is considered for a high-rate transmission. With an emphasis on a preamble design for multi-channel separation, we address a channel estimation based on the time-domain windowing and its imperfectness in MIMO-OFDM system. By properly designing each preamble for multiple antennas to be orthogonal in the time domain, the channel estimation can be executed based on semi-blind processing in the case of more than two transmitting antennas. And also we evaluate PAR performance in the MIMO-OFDM system using the SLM and PTS approaches. The investigated SLM and PTS schemes for MIMO-OFDM signals select the transmitted sequence with lowest average PAR over all transmitting antennas and retrieve the side information very accurately at the expense of a slight degradation of the PAR performance. The low probability of false side information can improve the overall detection performance of the MIMO-OFDM system with erroneous side information compared to the ordinary SLM and PTS approaches, respectively. Also, we provide closed form of the average BER performance in MIMO-OFDM system using analytic approach. [ABSTRACT FROM AUTHOR]