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A simple, specific, accurate and economic reverse phase liquid chromatographic method was developed for the estimation of Sofosbuvir in all polymorphic form. Mobile phase contains acetonitrile and 0.05M ammonium acetate (pH 7.6) in ratio of 50:50 (v/v). A logarithmic calibration curve was plotted from 10 µgmL-1 to 60 µgmL-1 (r = 0.9989) for sofosbuvir, with the limit of detection (LOD) and the limit of quantification (LOQ) of 0.245 µgmL-1 and 0.816 µgmL-1, respectively. The developed method was highly validated and practiced for the assurance of sofosbuvir raw materials with acceptable and non-objectionable accuracy and precision results (recovery 99-102%, RSD <1.2%, n = 3). Zero significant difference (t-test) was obtained between the overall results of the developed RP-HPLC method. [ABSTRACT FROM AUTHOR]

Chickpea wilt disease caused by Fusarium oxysporum f.sp. ciceris (Padwick) is a devastating disease. The best way to control chickpea wilt disease is to find resistant varieties/lines among available germplasm. Accordingly, three hundred and eighteen diverse chickpea genotypes were evaluated within sick plot conditions for disease severity against natural inoculum. The experiment was planted in an augmented design during two year investigating seasons. Disease severity was scored with a disease rating scale and area under disease progressive curve was recorded. A total of just three lines/varieties 5006, k021-10 and k035-10 were possessing high resistance in both investigation seasons. Among six biological control agents evaluated against pathogen in vitro by dual culture test showed that Pseudomonas fluorescens had more mycelial growth inhibition of pathogen (Foc) i.e., 70.94% over control. Trichoderma harzianum was proved to be second best followed by Rhizobia spp. and Bacillus subtilis with 63.95%, 60.79% and 57.68% growth reduction, respectively. In glass house assay, P. fluorescens was proved to be most effective, showed high disease reduction percentage with mean 76.78 over inoculated control. [ABSTRACT FROM AUTHOR]