1. Astragaloside IV Suppresses Hepatic Proliferation in Regenerating Rat Liver after 70% Partial Hepatectomy via Down-Regulation of Cell Cycle Pathway and DNA Replication.
- Author
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Lee, Gyeong-Seok, Jeong, Hee-Yeon, Yang, Hyeon-Gung, Seo, Young-Ran, Jung, Eui-Gil, Lee, Yong-Seok, Nam, Kung-Woo, Kim, Wan-Jong, Pezzani, Raffaele, and Vitalini, Sara
- Subjects
HEPATOCYTE growth factor ,CELL cycle ,ASTRAGALUS membranaceus ,LIVER regeneration ,HEPATECTOMY ,TRANSFORMING growth factors - Abstract
Astragaloside IV (AS-IV) is one of the major bio-active ingredients of huang qi which is the dried root of Astragalus membranaceus (a traditional Chinese medicinal plant). The pharmacological effects of AS-IV, including anti-oxidative, anti-cancer, and anti-diabetic effects have been actively studied, however, the effects of AS-IV on liver regeneration have not yet been fully described. Thus, the aim of this study was to explore the effects of AS-IV on regenerating liver after 70% partial hepatectomy (PHx) in rats. Differentially expressed mRNAs, proliferative marker and growth factors were analyzed. AS-IV (10 mg/kg) was administrated orally 2 h before surgery. We found 20 core genes showed effects of AS-IV, many of which were involved with functions related to DNA replication during cell division. AS-IV down-regulates MAPK signaling, PI3/Akt signaling, and cell cycle pathway. Hepatocyte growth factor (HGF) and cyclin D1 expression were also decreased by AS-IV administration. Transforming growth factor β1 (TGFβ1, growth regulation signal) was slightly increased. In short, AS-IV down-regulated proliferative signals and genes related to DNA replication. In conclusion, AS-IV showed anti-proliferative activity in regenerating liver tissue after 70% PHx. [ABSTRACT FROM AUTHOR]
- Published
- 2021
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